Danaus plexippus plexippus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia;

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15140 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A212ELK4|A0A212ELK4_DANPL Putative accessory gland protein (Fragment) OS=Danaus plexippus plexippus OX=278856 GN=KGM_207799B PE=4 SV=1
MM1 pKa = 7.99ADD3 pKa = 3.45IDD5 pKa = 3.98EE6 pKa = 4.51MNIGEE11 pKa = 4.51DD12 pKa = 2.95GGYY15 pKa = 10.55EE16 pKa = 4.08YY17 pKa = 9.21DD18 pKa = 3.38TTKK21 pKa = 10.22IPEE24 pKa = 4.8IITDD28 pKa = 3.87DD29 pKa = 3.94MNEE32 pKa = 3.74SGYY35 pKa = 10.31QEE37 pKa = 4.59GSEE40 pKa = 4.01KK41 pKa = 10.83SYY43 pKa = 11.51GDD45 pKa = 3.31RR46 pKa = 11.84RR47 pKa = 11.84DD48 pKa = 3.39SKK50 pKa = 10.75EE51 pKa = 4.13GYY53 pKa = 9.87GGEE56 pKa = 4.26GDD58 pKa = 3.78SDD60 pKa = 4.03SGVDD64 pKa = 4.17GVSSGCSTVDD74 pKa = 3.14DD75 pKa = 4.71CPVVSINDD83 pKa = 3.11VVAYY87 pKa = 10.11YY88 pKa = 10.24DD89 pKa = 3.08IRR91 pKa = 11.84PRR93 pKa = 11.84RR94 pKa = 11.84RR95 pKa = 11.84YY96 pKa = 9.21VPQLNFYY103 pKa = 9.87HH104 pKa = 7.51LDD106 pKa = 3.55IEE108 pKa = 4.55RR109 pKa = 11.84MAMDD113 pKa = 4.69AVVQKK118 pKa = 11.25YY119 pKa = 10.2DD120 pKa = 3.5FSDD123 pKa = 3.85LLSVAQSAPMRR134 pKa = 11.84IDD136 pKa = 3.69CDD138 pKa = 4.18CNTT141 pKa = 4.06

Molecular weight: 15.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A212EQ46|A0A212EQ46_DANPL Uncharacterized protein OS=Danaus plexippus plexippus OX=278856 GN=KGM_201592 PE=4 SV=1
MM1 pKa = 7.07VAGARR6 pKa = 11.84AGVRR10 pKa = 11.84VWVRR14 pKa = 11.84VRR16 pKa = 11.84VRR18 pKa = 11.84LRR20 pKa = 11.84LRR22 pKa = 11.84PWRR25 pKa = 11.84ADD27 pKa = 2.95GSRR30 pKa = 11.84LVVSVAVGIAAAAAPRR46 pKa = 11.84PAALPLSTTLL56 pKa = 4.2

Molecular weight: 5.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15140

0

15140

6413201

8

19648

423.6

47.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.742 ± 0.03

2.062 ± 0.037

5.619 ± 0.016

6.64 ± 0.03

3.542 ± 0.017

5.582 ± 0.025

2.506 ± 0.012

5.407 ± 0.02

6.361 ± 0.032

9.095 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.241 ± 0.012

4.884 ± 0.025

5.384 ± 0.028

3.827 ± 0.018

5.813 ± 0.028

7.833 ± 0.028

5.691 ± 0.022

6.383 ± 0.019

1.106 ± 0.009

3.282 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski