Amino acid dipepetide frequency for Pig stool associated circular ssDNA virus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.13AlaAla: 2.13 ± 1.45
3.195AlaCys: 3.195 ± 1.606
9.585AlaAsp: 9.585 ± 2.224
0.0AlaGlu: 0.0 ± 0.0
2.13AlaPhe: 2.13 ± 1.834
3.195AlaGly: 3.195 ± 2.26
0.0AlaHis: 0.0 ± 0.0
2.13AlaIle: 2.13 ± 1.14
2.13AlaLys: 2.13 ± 1.174
4.26AlaLeu: 4.26 ± 1.6
4.26AlaMet: 4.26 ± 1.866
2.13AlaAsn: 2.13 ± 1.699
3.195AlaPro: 3.195 ± 1.278
1.065AlaGln: 1.065 ± 1.199
0.0AlaArg: 0.0 ± 0.0
3.195AlaSer: 3.195 ± 1.54
3.195AlaThr: 3.195 ± 1.542
4.26AlaVal: 4.26 ± 1.6
1.065AlaTrp: 1.065 ± 0.917
1.065AlaTyr: 1.065 ± 1.193
0.0AlaXaa: 0.0 ± 0.0
Cys
1.065CysAla: 1.065 ± 0.725
0.0CysCys: 0.0 ± 0.0
1.065CysAsp: 1.065 ± 0.725
1.065CysGlu: 1.065 ± 0.917
0.0CysPhe: 0.0 ± 0.0
0.0CysGly: 0.0 ± 0.0
0.0CysHis: 0.0 ± 0.0
0.0CysIle: 0.0 ± 0.0
0.0CysLys: 0.0 ± 0.0
1.065CysLeu: 1.065 ± 1.109
0.0CysMet: 0.0 ± 0.0
1.065CysAsn: 1.065 ± 0.917
0.0CysPro: 0.0 ± 0.0
0.0CysGln: 0.0 ± 0.0
1.065CysArg: 1.065 ± 1.109
4.26CysSer: 4.26 ± 2.298
2.13CysThr: 2.13 ± 2.218
1.065CysVal: 1.065 ± 0.917
0.0CysTrp: 0.0 ± 0.0
1.065CysTyr: 1.065 ± 0.725
0.0CysXaa: 0.0 ± 0.0
Asp
2.13AspAla: 2.13 ± 1.174
0.0AspCys: 0.0 ± 0.0
3.195AspAsp: 3.195 ± 1.278
2.13AspGlu: 2.13 ± 1.834
2.13AspPhe: 2.13 ± 0.843
4.26AspGly: 4.26 ± 1.611
1.065AspHis: 1.065 ± 1.193
2.13AspIle: 2.13 ± 0.843
2.13AspLys: 2.13 ± 0.843
7.455AspLeu: 7.455 ± 1.465
2.13AspMet: 2.13 ± 2.069
1.065AspAsn: 1.065 ± 0.725
2.13AspPro: 2.13 ± 1.45
1.065AspGln: 1.065 ± 1.199
3.195AspArg: 3.195 ± 2.751
1.065AspSer: 1.065 ± 0.725
5.325AspThr: 5.325 ± 2.04
7.455AspVal: 7.455 ± 3.787
4.26AspTrp: 4.26 ± 2.715
4.26AspTyr: 4.26 ± 1.6
0.0AspXaa: 0.0 ± 0.0
Glu
1.065GluAla: 1.065 ± 0.725
0.0GluCys: 0.0 ± 0.0
0.0GluAsp: 0.0 ± 0.0
2.13GluGlu: 2.13 ± 1.834
2.13GluPhe: 2.13 ± 0.843
2.13GluGly: 2.13 ± 0.843
0.0GluHis: 0.0 ± 0.0
4.26GluIle: 4.26 ± 1.899
2.13GluLys: 2.13 ± 1.834
3.195GluLeu: 3.195 ± 1.606
0.0GluMet: 0.0 ± 0.0
2.13GluAsn: 2.13 ± 1.358
1.065GluPro: 1.065 ± 0.725
3.195GluGln: 3.195 ± 1.493
3.195GluArg: 3.195 ± 1.759
2.13GluSer: 2.13 ± 1.457
3.195GluThr: 3.195 ± 1.191
2.13GluVal: 2.13 ± 1.834
1.065GluTrp: 1.065 ± 0.917
2.13GluTyr: 2.13 ± 1.834
0.0GluXaa: 0.0 ± 0.0
Phe
0.0PheAla: 0.0 ± 0.0
0.0PheCys: 0.0 ± 0.0
3.195PheAsp: 3.195 ± 1.278
3.195PheGlu: 3.195 ± 2.751
2.13PhePhe: 2.13 ± 1.45
3.195PheGly: 3.195 ± 0.857
1.065PheHis: 1.065 ± 1.193
0.0PheIle: 0.0 ± 0.0
3.195PheLys: 3.195 ± 2.252
3.195PheLeu: 3.195 ± 2.326
2.13PheMet: 2.13 ± 1.059
3.195PheAsn: 3.195 ± 1.278
2.13PhePro: 2.13 ± 1.479
2.13PheGln: 2.13 ± 1.14
6.39PheArg: 6.39 ± 3.412
4.26PheSer: 4.26 ± 1.028
4.26PheThr: 4.26 ± 2.109
3.195PheVal: 3.195 ± 1.501
1.065PheTrp: 1.065 ± 0.725
2.13PheTyr: 2.13 ± 0.843
0.0PheXaa: 0.0 ± 0.0
Gly
4.26GlyAla: 4.26 ± 2.387
1.065GlyCys: 1.065 ± 0.917
2.13GlyAsp: 2.13 ± 1.174
1.065GlyGlu: 1.065 ± 1.193
1.065GlyPhe: 1.065 ± 1.109
4.26GlyGly: 4.26 ± 2.452
1.065GlyHis: 1.065 ± 0.725
4.26GlyIle: 4.26 ± 1.635
5.325GlyLys: 5.325 ± 2.04
10.65GlyLeu: 10.65 ± 2.894
1.065GlyMet: 1.065 ± 0.725
1.065GlyAsn: 1.065 ± 0.917
1.065GlyPro: 1.065 ± 0.725
0.0GlyGln: 0.0 ± 0.0
5.325GlyArg: 5.325 ± 2.392
1.065GlySer: 1.065 ± 1.109
4.26GlyThr: 4.26 ± 2.106
2.13GlyVal: 2.13 ± 0.843
3.195GlyTrp: 3.195 ± 2.26
3.195GlyTyr: 3.195 ± 2.751
0.0GlyXaa: 0.0 ± 0.0
His
0.0HisAla: 0.0 ± 0.0
0.0HisCys: 0.0 ± 0.0
0.0HisAsp: 0.0 ± 0.0
0.0HisGlu: 0.0 ± 0.0
0.0HisPhe: 0.0 ± 0.0
0.0HisGly: 0.0 ± 0.0
0.0HisHis: 0.0 ± 0.0
1.065HisIle: 1.065 ± 0.917
0.0HisLys: 0.0 ± 0.0
1.065HisLeu: 1.065 ± 1.109
1.065HisMet: 1.065 ± 0.917
1.065HisAsn: 1.065 ± 1.199
1.065HisPro: 1.065 ± 1.109
1.065HisGln: 1.065 ± 0.725
5.325HisArg: 5.325 ± 2.794
1.065HisSer: 1.065 ± 1.193
2.13HisThr: 2.13 ± 1.173
1.065HisVal: 1.065 ± 0.917
0.0HisTrp: 0.0 ± 0.0
1.065HisTyr: 1.065 ± 0.917
0.0HisXaa: 0.0 ± 0.0
Ile
4.26IleAla: 4.26 ± 1.899
1.065IleCys: 1.065 ± 1.109
2.13IleAsp: 2.13 ± 1.358
5.325IleGlu: 5.325 ± 1.497
0.0IlePhe: 0.0 ± 0.0
5.325IleGly: 5.325 ± 2.641
2.13IleHis: 2.13 ± 1.173
3.195IleIle: 3.195 ± 2.122
0.0IleLys: 0.0 ± 0.0
7.455IleLeu: 7.455 ± 3.795
2.13IleMet: 2.13 ± 1.45
3.195IleAsn: 3.195 ± 1.501
3.195IlePro: 3.195 ± 1.191
0.0IleGln: 0.0 ± 0.0
3.195IleArg: 3.195 ± 2.447
4.26IleSer: 4.26 ± 1.611
2.13IleThr: 2.13 ± 1.834
2.13IleVal: 2.13 ± 1.173
1.065IleTrp: 1.065 ± 0.917
0.0IleTyr: 0.0 ± 0.0
0.0IleXaa: 0.0 ± 0.0
Lys
3.195LysAla: 3.195 ± 1.062
0.0LysCys: 0.0 ± 0.0
4.26LysAsp: 4.26 ± 1.21
0.0LysGlu: 0.0 ± 0.0
1.065LysPhe: 1.065 ± 0.725
1.065LysGly: 1.065 ± 0.917
2.13LysHis: 2.13 ± 1.457
1.065LysIle: 1.065 ± 0.725
1.065LysLys: 1.065 ± 0.725
6.39LysLeu: 6.39 ± 1.838
1.065LysMet: 1.065 ± 0.725
5.325LysAsn: 5.325 ± 2.327
0.0LysPro: 0.0 ± 0.0
0.0LysGln: 0.0 ± 0.0
2.13LysArg: 2.13 ± 1.834
3.195LysSer: 3.195 ± 2.252
4.26LysThr: 4.26 ± 1.329
3.195LysVal: 3.195 ± 1.191
0.0LysTrp: 0.0 ± 0.0
3.195LysTyr: 3.195 ± 2.392
0.0LysXaa: 0.0 ± 0.0
Leu
3.195LeuAla: 3.195 ± 0.857
2.13LeuCys: 2.13 ± 1.149
3.195LeuAsp: 3.195 ± 1.982
3.195LeuGlu: 3.195 ± 1.759
2.13LeuPhe: 2.13 ± 1.14
7.455LeuGly: 7.455 ± 1.413
2.13LeuHis: 2.13 ± 1.605
3.195LeuIle: 3.195 ± 1.501
4.26LeuLys: 4.26 ± 2.475
8.52LeuLeu: 8.52 ± 6.507
0.0LeuMet: 0.0 ± 0.0
3.195LeuAsn: 3.195 ± 2.175
6.39LeuPro: 6.39 ± 1.557
7.455LeuGln: 7.455 ± 2.436
3.195LeuArg: 3.195 ± 2.064
12.78LeuSer: 12.78 ± 3.196
7.455LeuThr: 7.455 ± 4.226
6.39LeuVal: 6.39 ± 1.367
3.195LeuTrp: 3.195 ± 0.857
7.455LeuTyr: 7.455 ± 3.242
0.0LeuXaa: 0.0 ± 0.0
Met
1.065MetAla: 1.065 ± 0.725
0.0MetCys: 0.0 ± 0.0
4.26MetAsp: 4.26 ± 2.387
1.065MetGlu: 1.065 ± 0.725
1.065MetPhe: 1.065 ± 0.725
2.13MetGly: 2.13 ± 1.174
0.0MetHis: 0.0 ± 0.0
0.0MetIle: 0.0 ± 0.0
1.065MetLys: 1.065 ± 0.917
1.065MetLeu: 1.065 ± 0.725
0.0MetMet: 0.0 ± 0.0
0.0MetAsn: 0.0 ± 0.0
4.26MetPro: 4.26 ± 1.329
4.26MetGln: 4.26 ± 2.901
2.13MetArg: 2.13 ± 1.45
1.065MetSer: 1.065 ± 1.109
2.13MetThr: 2.13 ± 2.218
4.26MetVal: 4.26 ± 2.901
0.0MetTrp: 0.0 ± 0.0
1.065MetTyr: 1.065 ± 0.917
0.0MetXaa: 0.0 ± 0.0
Asn
2.13AsnAla: 2.13 ± 1.358
1.065AsnCys: 1.065 ± 0.725
3.195AsnAsp: 3.195 ± 1.606
0.0AsnGlu: 0.0 ± 0.0
5.325AsnPhe: 5.325 ± 1.274
3.195AsnGly: 3.195 ± 1.775
1.065AsnHis: 1.065 ± 0.917
3.195AsnIle: 3.195 ± 1.501
2.13AsnLys: 2.13 ± 2.399
1.065AsnLeu: 1.065 ± 1.199
0.0AsnMet: 0.0 ± 0.0
1.065AsnAsn: 1.065 ± 0.725
2.13AsnPro: 2.13 ± 1.173
0.0AsnGln: 0.0 ± 0.0
4.26AsnArg: 4.26 ± 2.101
5.325AsnSer: 5.325 ± 2.04
3.195AsnThr: 3.195 ± 2.175
0.0AsnVal: 0.0 ± 0.0
0.0AsnTrp: 0.0 ± 0.0
1.065AsnTyr: 1.065 ± 0.917
0.0AsnXaa: 0.0 ± 0.0
Pro
4.26ProAla: 4.26 ± 2.101
4.26ProCys: 4.26 ± 3.197
3.195ProAsp: 3.195 ± 0.857
6.39ProGlu: 6.39 ± 2.364
4.26ProPhe: 4.26 ± 0.987
1.065ProGly: 1.065 ± 0.725
1.065ProHis: 1.065 ± 0.917
3.195ProIle: 3.195 ± 1.297
2.13ProLys: 2.13 ± 2.386
6.39ProLeu: 6.39 ± 2.369
2.13ProMet: 2.13 ± 1.14
1.065ProAsn: 1.065 ± 0.725
3.195ProPro: 3.195 ± 1.278
2.13ProGln: 2.13 ± 1.45
4.26ProArg: 4.26 ± 1.687
4.26ProSer: 4.26 ± 2.345
1.065ProThr: 1.065 ± 0.917
1.065ProVal: 1.065 ± 1.199
0.0ProTrp: 0.0 ± 0.0
0.0ProTyr: 0.0 ± 0.0
0.0ProXaa: 0.0 ± 0.0
Gln
2.13GlnAla: 2.13 ± 1.174
0.0GlnCys: 0.0 ± 0.0
3.195GlnAsp: 3.195 ± 1.278
1.065GlnGlu: 1.065 ± 1.199
4.26GlnPhe: 4.26 ± 1.329
3.195GlnGly: 3.195 ± 2.175
1.065GlnHis: 1.065 ± 1.109
3.195GlnIle: 3.195 ± 1.435
0.0GlnLys: 0.0 ± 0.0
5.325GlnLeu: 5.325 ± 1.319
1.065GlnMet: 1.065 ± 0.725
1.065GlnAsn: 1.065 ± 0.917
0.0GlnPro: 0.0 ± 0.0
1.065GlnGln: 1.065 ± 1.199
3.195GlnArg: 3.195 ± 1.493
3.195GlnSer: 3.195 ± 1.54
1.065GlnThr: 1.065 ± 0.725
4.26GlnVal: 4.26 ± 1.329
1.065GlnTrp: 1.065 ± 1.109
2.13GlnTyr: 2.13 ± 1.479
0.0GlnXaa: 0.0 ± 0.0
Arg
3.195ArgAla: 3.195 ± 1.982
0.0ArgCys: 0.0 ± 0.0
4.26ArgAsp: 4.26 ± 2.774
1.065ArgGlu: 1.065 ± 0.917
3.195ArgPhe: 3.195 ± 1.555
4.26ArgGly: 4.26 ± 1.773
2.13ArgHis: 2.13 ± 1.149
7.455ArgIle: 7.455 ± 1.706
1.065ArgLys: 1.065 ± 0.917
4.26ArgLeu: 4.26 ± 2.143
2.13ArgMet: 2.13 ± 1.14
2.13ArgAsn: 2.13 ± 1.457
4.26ArgPro: 4.26 ± 2.914
2.13ArgGln: 2.13 ± 1.699
4.26ArgArg: 4.26 ± 2.957
6.39ArgSer: 6.39 ± 3.205
3.195ArgThr: 3.195 ± 2.064
3.195ArgVal: 3.195 ± 1.759
1.065ArgTrp: 1.065 ± 1.109
2.13ArgTyr: 2.13 ± 1.834
0.0ArgXaa: 0.0 ± 0.0
Ser
6.39SerAla: 6.39 ± 2.152
1.065SerCys: 1.065 ± 1.109
5.325SerAsp: 5.325 ± 4.702
3.195SerGlu: 3.195 ± 0.857
6.39SerPhe: 6.39 ± 4.541
5.325SerGly: 5.325 ± 1.684
1.065SerHis: 1.065 ± 0.917
4.26SerIle: 4.26 ± 0.987
5.325SerLys: 5.325 ± 2.392
6.39SerLeu: 6.39 ± 2.578
3.195SerMet: 3.195 ± 2.175
5.325SerAsn: 5.325 ± 1.274
6.39SerPro: 6.39 ± 2.777
4.26SerGln: 4.26 ± 3.33
1.065SerArg: 1.065 ± 1.109
5.325SerSer: 5.325 ± 4.286
10.65SerThr: 10.65 ± 5.178
7.455SerVal: 7.455 ± 2.383
2.13SerTrp: 2.13 ± 1.149
0.0SerTyr: 0.0 ± 0.0
0.0SerXaa: 0.0 ± 0.0
Thr
3.195ThrAla: 3.195 ± 1.191
0.0ThrCys: 0.0 ± 0.0
4.26ThrAsp: 4.26 ± 2.106
2.13ThrGlu: 2.13 ± 1.45
1.065ThrPhe: 1.065 ± 1.109
1.065ThrGly: 1.065 ± 1.193
0.0ThrHis: 0.0 ± 0.0
4.26ThrIle: 4.26 ± 1.361
2.13ThrLys: 2.13 ± 1.173
6.39ThrLeu: 6.39 ± 3.05
2.13ThrMet: 2.13 ± 1.45
0.0ThrAsn: 0.0 ± 0.0
9.585ThrPro: 9.585 ± 2.115
4.26ThrGln: 4.26 ± 1.899
4.26ThrArg: 4.26 ± 3.108
11.715ThrSer: 11.715 ± 2.533
8.52ThrThr: 8.52 ± 2.985
7.455ThrVal: 7.455 ± 6.485
1.065ThrTrp: 1.065 ± 0.725
1.065ThrTyr: 1.065 ± 0.725
0.0ThrXaa: 0.0 ± 0.0
Val
4.26ValAla: 4.26 ± 1.687
0.0ValCys: 0.0 ± 0.0
1.065ValAsp: 1.065 ± 0.725
1.065ValGlu: 1.065 ± 0.725
6.39ValPhe: 6.39 ± 1.794
3.195ValGly: 3.195 ± 1.54
1.065ValHis: 1.065 ± 1.109
2.13ValIle: 2.13 ± 1.699
2.13ValLys: 2.13 ± 1.174
8.52ValLeu: 8.52 ± 1.761
4.26ValMet: 4.26 ± 1.134
4.26ValAsn: 4.26 ± 2.901
3.195ValPro: 3.195 ± 1.435
3.195ValGln: 3.195 ± 1.606
3.195ValArg: 3.195 ± 1.606
8.52ValSer: 8.52 ± 2.925
3.195ValThr: 3.195 ± 1.278
1.065ValVal: 1.065 ± 0.725
1.065ValTrp: 1.065 ± 0.917
3.195ValTyr: 3.195 ± 1.606
0.0ValXaa: 0.0 ± 0.0
Trp
2.13TrpAla: 2.13 ± 1.834
0.0TrpCys: 0.0 ± 0.0
1.065TrpAsp: 1.065 ± 0.917
1.065TrpGlu: 1.065 ± 0.917
3.195TrpPhe: 3.195 ± 2.484
2.13TrpGly: 2.13 ± 1.14
0.0TrpHis: 0.0 ± 0.0
0.0TrpIle: 0.0 ± 0.0
4.26TrpLys: 4.26 ± 1.21
1.065TrpLeu: 1.065 ± 1.109
0.0TrpMet: 0.0 ± 0.0
0.0TrpAsn: 0.0 ± 0.0
0.0TrpPro: 0.0 ± 0.0
1.065TrpGln: 1.065 ± 0.917
0.0TrpArg: 0.0 ± 0.0
2.13TrpSer: 2.13 ± 1.358
1.065TrpThr: 1.065 ± 0.725
0.0TrpVal: 0.0 ± 0.0
0.0TrpTrp: 0.0 ± 0.0
2.13TrpTyr: 2.13 ± 1.358
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.13TyrAla: 2.13 ± 0.843
1.065TyrCys: 1.065 ± 0.917
1.065TyrAsp: 1.065 ± 0.725
2.13TyrGlu: 2.13 ± 1.834
2.13TyrPhe: 2.13 ± 1.834
1.065TyrGly: 1.065 ± 0.725
0.0TyrHis: 0.0 ± 0.0
3.195TyrIle: 3.195 ± 1.982
2.13TyrLys: 2.13 ± 0.843
3.195TyrLeu: 3.195 ± 1.982
1.065TyrMet: 1.065 ± 0.975
1.065TyrAsn: 1.065 ± 1.199
2.13TyrPro: 2.13 ± 1.174
3.195TyrGln: 3.195 ± 1.606
2.13TyrArg: 2.13 ± 1.174
5.325TyrSer: 5.325 ± 1.289
2.13TyrThr: 2.13 ± 1.479
3.195TyrVal: 3.195 ± 1.606
0.0TyrTrp: 0.0 ± 0.0
2.13TyrTyr: 2.13 ± 1.45
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5 proteins (940 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski