Tremella mesenterica (Jelly fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Tremellales; Tremellaceae; Tremella

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8061 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q1BRF8|A0A4Q1BRF8_TREME Uncharacterized protein OS=Tremella mesenterica OX=5217 GN=M231_02218 PE=4 SV=1
MM1 pKa = 8.28DD2 pKa = 6.76DD3 pKa = 5.06DD4 pKa = 3.95TTGWLRR10 pKa = 11.84YY11 pKa = 9.12SVQPLPEE18 pKa = 5.1DD19 pKa = 3.62SNNTEE24 pKa = 4.48GCPSAYY30 pKa = 10.3YY31 pKa = 10.1VIQPLADD38 pKa = 4.97DD39 pKa = 4.48GTHH42 pKa = 5.67LTSTVHH48 pKa = 7.11LKK50 pKa = 10.4QEE52 pKa = 4.63DD53 pKa = 3.79KK54 pKa = 11.33DD55 pKa = 4.0GTTMTTVKK63 pKa = 10.71GGAFTLNSFGPEE75 pKa = 4.06FEE77 pKa = 6.34AIDD80 pKa = 3.73PTSIRR85 pKa = 11.84DD86 pKa = 3.64GSGLGEE92 pKa = 4.59NILFQYY98 pKa = 10.09RR99 pKa = 11.84PSSFIDD105 pKa = 4.66FYY107 pKa = 11.71DD108 pKa = 4.18DD109 pKa = 3.52NNVIADD115 pKa = 4.51AVACLQDD122 pKa = 3.16IPMPSSFSLGVVDD135 pKa = 3.67WTVCSTPATTLGNQGQWLRR154 pKa = 11.84CTEE157 pKa = 4.0SMTVCAITPEE167 pKa = 4.13VGGGGEE173 pKa = 4.2RR174 pKa = 11.84GQEE177 pKa = 4.09SLVGAVEE184 pKa = 4.06VMEE187 pKa = 4.33II188 pKa = 3.78

Molecular weight: 20.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q1BIT3|A0A4Q1BIT3_TREME Uncharacterized protein OS=Tremella mesenterica OX=5217 GN=M231_05171 PE=4 SV=1
MM1 pKa = 7.62CIVYY5 pKa = 9.6IRR7 pKa = 11.84KK8 pKa = 9.48VSTTSRR14 pKa = 11.84SLPVARR20 pKa = 11.84SPLPPAARR28 pKa = 11.84RR29 pKa = 11.84SVFATEE35 pKa = 4.54SPSPSDD41 pKa = 4.23EE42 pKa = 4.62LPGLPPPLPPSVSFRR57 pKa = 11.84AARR60 pKa = 11.84PGAPLWEE67 pKa = 4.95LSPSPPPRR75 pKa = 11.84GPRR78 pKa = 11.84PPRR81 pKa = 11.84GPRR84 pKa = 11.84PPRR87 pKa = 11.84APPVVNLCSPSPIVPGAFVVPAPPSPPRR115 pKa = 11.84PSPAPPLSSSSSSEE129 pKa = 3.72EE130 pKa = 3.85VPPRR134 pKa = 11.84TQEE137 pKa = 4.09EE138 pKa = 4.5IIADD142 pKa = 3.74LRR144 pKa = 11.84EE145 pKa = 4.16LVEE148 pKa = 5.53VLTLDD153 pKa = 3.43NLKK156 pKa = 9.32TEE158 pKa = 4.09KK159 pKa = 10.35RR160 pKa = 11.84LAAMTVVALRR170 pKa = 11.84AADD173 pKa = 3.56MMDD176 pKa = 3.22VQGVIIRR183 pKa = 11.84RR184 pKa = 11.84FLNQIRR190 pKa = 11.84LSTRR194 pKa = 11.84NLSKK198 pKa = 10.52KK199 pKa = 9.81SSHH202 pKa = 6.62IGSATHH208 pKa = 7.0SLLLQWNEE216 pKa = 3.39LLLRR220 pKa = 11.84TGCHH224 pKa = 6.78AIFGHH229 pKa = 6.0KK230 pKa = 9.61FWGEE234 pKa = 3.59INDD237 pKa = 4.36TIRR240 pKa = 11.84QKK242 pKa = 10.91EE243 pKa = 3.74RR244 pKa = 11.84DD245 pKa = 3.61LTGVAVAGPSTQGLRR260 pKa = 11.84DD261 pKa = 3.58EE262 pKa = 4.51VRR264 pKa = 11.84AASEE268 pKa = 4.45TFWDD272 pKa = 4.02ANWRR276 pKa = 11.84MRR278 pKa = 11.84NIRR281 pKa = 11.84GVDD284 pKa = 3.37VSLGQGTRR292 pKa = 11.84RR293 pKa = 11.84GTTANYY299 pKa = 9.7AVVISRR305 pKa = 11.84VCVEE309 pKa = 4.28LLGGSSPP316 pKa = 4.02

Molecular weight: 34.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8061

0

8061

3984402

45

4952

494.3

54.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.996 ± 0.029

0.986 ± 0.009

5.561 ± 0.02

6.5 ± 0.024

3.204 ± 0.015

6.865 ± 0.027

2.483 ± 0.013

4.9 ± 0.017

4.849 ± 0.024

9.053 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.203 ± 0.009

3.495 ± 0.016

7.048 ± 0.033

3.94 ± 0.018

6.252 ± 0.022

9.583 ± 0.039

6.324 ± 0.017

6.022 ± 0.019

1.346 ± 0.01

2.391 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski