Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacteraceae; Thermoanaerobacter; Thermoanaerobacter pseudethanolicus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2193 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0KD88|B0KD88_THEP3 Phosphotransferase system lactose/cellobiose-specific IIB subunit OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=Teth39_1981 PE=4 SV=1
MM1 pKa = 7.61EE2 pKa = 5.14KK3 pKa = 9.49WQCTVCGYY11 pKa = 10.27IYY13 pKa = 10.44DD14 pKa = 4.32PEE16 pKa = 5.42EE17 pKa = 4.56GDD19 pKa = 3.38PSQGIEE25 pKa = 3.79PGTPFEE31 pKa = 4.71EE32 pKa = 5.8LPDD35 pKa = 3.91DD36 pKa = 4.55WVCPDD41 pKa = 4.03CGVGKK46 pKa = 10.52DD47 pKa = 3.67MFEE50 pKa = 4.32KK51 pKa = 10.17MM52 pKa = 4.2

Molecular weight: 5.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B0K8K7|MURG_THEP3 UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=murG PE=3 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.82QPKK8 pKa = 9.4KK9 pKa = 7.87RR10 pKa = 11.84HH11 pKa = 5.46RR12 pKa = 11.84KK13 pKa = 8.28KK14 pKa = 9.71VHH16 pKa = 5.38GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTKK25 pKa = 10.09SGRR28 pKa = 11.84NVLKK32 pKa = 10.5RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.4GRR39 pKa = 11.84HH40 pKa = 5.53RR41 pKa = 11.84LTAA44 pKa = 4.06

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2193

0

2193

664459

31

2887

303.0

34.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.37 ± 0.051

0.793 ± 0.018

5.233 ± 0.037

7.966 ± 0.061

4.238 ± 0.042

6.793 ± 0.047

1.431 ± 0.02

9.555 ± 0.047

8.939 ± 0.045

9.269 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.56 ± 0.025

4.861 ± 0.041

3.451 ± 0.029

2.539 ± 0.03

3.957 ± 0.042

5.272 ± 0.04

4.762 ± 0.035

7.237 ± 0.042

0.725 ± 0.019

4.049 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski