Amino acid dipepetide frequency for Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.003AlaAla: 5.003 ± 0.123	0.634AlaCys: 0.634 ± 0.031	2.882AlaAsp: 2.882 ± 0.07	4.224AlaGlu: 4.224 ± 0.096	2.671AlaPhe: 2.671 ± 0.068	4.443AlaGly: 4.443 ± 0.09	0.951AlaHis: 0.951 ± 0.041	6.345AlaIle: 6.345 ± 0.112	5.532AlaLys: 5.532 ± 0.106	6.631AlaLeu: 6.631 ± 0.113	1.72AlaMet: 1.72 ± 0.054	2.593AlaAsn: 2.593 ± 0.059	1.646AlaPro: 1.646 ± 0.057	1.768AlaGln: 1.768 ± 0.058	2.459AlaArg: 2.459 ± 0.062	3.222AlaSer: 3.222 ± 0.065	2.927AlaThr: 2.927 ± 0.064	5.203AlaVal: 5.203 ± 0.107	0.436AlaTrp: 0.436 ± 0.027	2.406AlaTyr: 2.406 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.486CysAla: 0.486 ± 0.03	0.062CysCys: 0.062 ± 0.011	0.524CysAsp: 0.524 ± 0.031	0.557CysGlu: 0.557 ± 0.03	0.266CysPhe: 0.266 ± 0.024	0.862CysGly: 0.862 ± 0.044	0.147CysHis: 0.147 ± 0.013	0.628CysIle: 0.628 ± 0.029	0.576CysLys: 0.576 ± 0.034	0.527CysLeu: 0.527 ± 0.03	0.16CysMet: 0.16 ± 0.014	0.39CysAsn: 0.39 ± 0.025	0.488CysPro: 0.488 ± 0.031	0.202CysGln: 0.202 ± 0.02	0.342CysArg: 0.342 ± 0.02	0.43CysSer: 0.43 ± 0.027	0.421CysThr: 0.421 ± 0.023	0.482CysVal: 0.482 ± 0.027	0.063CysTrp: 0.063 ± 0.01	0.313CysTyr: 0.313 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	2.859AspAla: 2.859 ± 0.063	0.385AspCys: 0.385 ± 0.021	2.139AspAsp: 2.139 ± 0.063	4.297AspGlu: 4.297 ± 0.082	2.778AspPhe: 2.778 ± 0.075	3.048AspGly: 3.048 ± 0.095	0.57AspHis: 0.57 ± 0.031	6.113AspIle: 6.113 ± 0.106	5.146AspLys: 5.146 ± 0.087	4.972AspLeu: 4.972 ± 0.086	1.345AspMet: 1.345 ± 0.043	2.37AspAsn: 2.37 ± 0.066	1.758AspPro: 1.758 ± 0.057	0.769AspGln: 0.769 ± 0.032	2.115AspArg: 2.115 ± 0.062	2.313AspSer: 2.313 ± 0.066	2.37AspThr: 2.37 ± 0.064	4.235AspVal: 4.235 ± 0.079	0.403AspTrp: 0.403 ± 0.028	2.339AspTyr: 2.339 ± 0.073	0.0AspXaa: 0.0 ± 0.0
Glu	4.954GluAla: 4.954 ± 0.111	0.439GluCys: 0.439 ± 0.03	4.143GluAsp: 4.143 ± 0.085	8.873GluGlu: 8.873 ± 0.152	2.909GluPhe: 2.909 ± 0.067	5.124GluGly: 5.124 ± 0.103	0.974GluHis: 0.974 ± 0.042	8.398GluIle: 8.398 ± 0.13	8.581GluLys: 8.581 ± 0.16	6.83GluLeu: 6.83 ± 0.112	2.2GluMet: 2.2 ± 0.057	4.163GluAsn: 4.163 ± 0.072	1.6GluPro: 1.6 ± 0.048	1.734GluGln: 1.734 ± 0.055	3.4GluArg: 3.4 ± 0.088	2.926GluSer: 2.926 ± 0.061	2.793GluThr: 2.793 ± 0.068	5.988GluVal: 5.988 ± 0.094	0.531GluTrp: 0.531 ± 0.029	3.103GluTyr: 3.103 ± 0.075	0.0GluXaa: 0.0 ± 0.0
Phe	2.707PheAla: 2.707 ± 0.074	0.379PheCys: 0.379 ± 0.023	2.563PheAsp: 2.563 ± 0.068	3.151PheGlu: 3.151 ± 0.077	1.898PhePhe: 1.898 ± 0.062	2.987PheGly: 2.987 ± 0.065	0.6PheHis: 0.6 ± 0.029	3.939PheIle: 3.939 ± 0.084	3.34PheLys: 3.34 ± 0.081	4.224PheLeu: 4.224 ± 0.086	1.127PheMet: 1.127 ± 0.045	2.069PheAsn: 2.069 ± 0.061	1.315PhePro: 1.315 ± 0.043	0.843PheGln: 0.843 ± 0.043	1.361PheArg: 1.361 ± 0.043	2.704PheSer: 2.704 ± 0.07	2.101PheThr: 2.101 ± 0.052	2.954PheVal: 2.954 ± 0.071	0.295PheTrp: 0.295 ± 0.027	1.823PheTyr: 1.823 ± 0.052	0.0PheXaa: 0.0 ± 0.0
Gly	4.428GlyAla: 4.428 ± 0.1	0.721GlyCys: 0.721 ± 0.034	3.382GlyAsp: 3.382 ± 0.081	4.431GlyGlu: 4.431 ± 0.087	3.072GlyPhe: 3.072 ± 0.078	4.769GlyGly: 4.769 ± 0.09	1.142GlyHis: 1.142 ± 0.044	7.445GlyIle: 7.445 ± 0.116	5.928GlyLys: 5.928 ± 0.102	6.046GlyLeu: 6.046 ± 0.103	1.806GlyMet: 1.806 ± 0.065	2.856GlyAsn: 2.856 ± 0.072	1.815GlyPro: 1.815 ± 0.056	1.567GlyGln: 1.567 ± 0.05	2.837GlyArg: 2.837 ± 0.067	3.245GlySer: 3.245 ± 0.077	3.49GlyThr: 3.49 ± 0.081	5.161GlyVal: 5.161 ± 0.092	0.549GlyTrp: 0.549 ± 0.032	3.246GlyTyr: 3.246 ± 0.074	0.0GlyXaa: 0.0 ± 0.0
His	0.865HisAla: 0.865 ± 0.035	0.163HisCys: 0.163 ± 0.016	0.697HisAsp: 0.697 ± 0.036	0.868HisGlu: 0.868 ± 0.035	0.659HisPhe: 0.659 ± 0.03	1.118HisGly: 1.118 ± 0.039	0.327HisHis: 0.327 ± 0.025	1.365HisIle: 1.365 ± 0.043	1.043HisLys: 1.043 ± 0.039	1.272HisLeu: 1.272 ± 0.042	0.301HisMet: 0.301 ± 0.022	0.691HisAsn: 0.691 ± 0.028	0.84HisPro: 0.84 ± 0.039	0.382HisGln: 0.382 ± 0.026	0.593HisArg: 0.593 ± 0.03	0.784HisSer: 0.784 ± 0.033	0.751HisThr: 0.751 ± 0.033	0.918HisVal: 0.918 ± 0.04	0.129HisTrp: 0.129 ± 0.015	0.548HisTyr: 0.548 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	6.952IleAla: 6.952 ± 0.115	0.703IleCys: 0.703 ± 0.036	5.591IleAsp: 5.591 ± 0.11	7.689IleGlu: 7.689 ± 0.128	3.881IlePhe: 3.881 ± 0.089	6.512IleGly: 6.512 ± 0.112	1.272IleHis: 1.272 ± 0.04	8.128IleIle: 8.128 ± 0.127	8.733IleLys: 8.733 ± 0.117	8.821IleLeu: 8.821 ± 0.131	2.199IleMet: 2.199 ± 0.058	4.658IleAsn: 4.658 ± 0.089	3.972IlePro: 3.972 ± 0.073	2.116IleGln: 2.116 ± 0.057	3.281IleArg: 3.281 ± 0.075	5.748IleSer: 5.748 ± 0.105	4.709IleThr: 4.709 ± 0.084	6.854IleVal: 6.854 ± 0.116	0.563IleTrp: 0.563 ± 0.029	3.174IleTyr: 3.174 ± 0.069	0.0IleXaa: 0.0 ± 0.0
Lys	5.525LysAla: 5.525 ± 0.098	0.539LysCys: 0.539 ± 0.037	5.2LysAsp: 5.2 ± 0.095	9.518LysGlu: 9.518 ± 0.153	3.118LysPhe: 3.118 ± 0.077	5.859LysGly: 5.859 ± 0.101	1.044LysHis: 1.044 ± 0.034	8.186LysIle: 8.186 ± 0.118	7.994LysLys: 7.994 ± 0.121	7.295LysLeu: 7.295 ± 0.115	2.303LysMet: 2.303 ± 0.053	5.121LysAsn: 5.121 ± 0.098	2.65LysPro: 2.65 ± 0.066	2.024LysGln: 2.024 ± 0.059	3.892LysArg: 3.892 ± 0.086	4.265LysSer: 4.265 ± 0.082	4.009LysThr: 4.009 ± 0.078	6.386LysVal: 6.386 ± 0.119	0.638LysTrp: 0.638 ± 0.035	3.827LysTyr: 3.827 ± 0.087	0.0LysXaa: 0.0 ± 0.0
Leu	5.717LeuAla: 5.717 ± 0.102	0.671LeuCys: 0.671 ± 0.034	4.685LeuAsp: 4.685 ± 0.097	6.852LeuGlu: 6.852 ± 0.113	3.653LeuPhe: 3.653 ± 0.097	6.149LeuGly: 6.149 ± 0.105	1.204LeuHis: 1.204 ± 0.044	8.157LeuIle: 8.157 ± 0.125	9.736LeuLys: 9.736 ± 0.124	8.539LeuLeu: 8.539 ± 0.169	2.321LeuMet: 2.321 ± 0.064	4.917LeuAsn: 4.917 ± 0.094	3.571LeuPro: 3.571 ± 0.081	2.528LeuGln: 2.528 ± 0.072	3.659LeuArg: 3.659 ± 0.08	6.085LeuSer: 6.085 ± 0.113	4.507LeuThr: 4.507 ± 0.078	5.653LeuVal: 5.653 ± 0.093	0.667LeuTrp: 0.667 ± 0.038	3.418LeuTyr: 3.418 ± 0.077	0.0LeuXaa: 0.0 ± 0.0
Met	1.975MetAla: 1.975 ± 0.052	0.185MetCys: 0.185 ± 0.016	1.359MetAsp: 1.359 ± 0.044	1.994MetGlu: 1.994 ± 0.069	0.918MetPhe: 0.918 ± 0.04	1.857MetGly: 1.857 ± 0.056	0.36MetHis: 0.36 ± 0.024	2.104MetIle: 2.104 ± 0.053	2.276MetLys: 2.276 ± 0.055	2.318MetLeu: 2.318 ± 0.057	0.754MetMet: 0.754 ± 0.04	1.066MetAsn: 1.066 ± 0.038	1.064MetPro: 1.064 ± 0.038	0.683MetGln: 0.683 ± 0.03	1.195MetArg: 1.195 ± 0.046	1.397MetSer: 1.397 ± 0.045	1.261MetThr: 1.261 ± 0.044	1.764MetVal: 1.764 ± 0.056	0.202MetTrp: 0.202 ± 0.017	0.868MetTyr: 0.868 ± 0.037	0.0MetXaa: 0.0 ± 0.0
Asn	2.796AsnAla: 2.796 ± 0.067	0.473AsnCys: 0.473 ± 0.029	2.099AsnAsp: 2.099 ± 0.073	3.275AsnGlu: 3.275 ± 0.082	2.241AsnPhe: 2.241 ± 0.067	2.942AsnGly: 2.942 ± 0.076	0.62AsnHis: 0.62 ± 0.036	5.219AsnIle: 5.219 ± 0.102	4.024AsnLys: 4.024 ± 0.086	4.906AsnLeu: 4.906 ± 0.085	1.308AsnMet: 1.308 ± 0.049	2.412AsnAsn: 2.412 ± 0.071	2.218AsnPro: 2.218 ± 0.064	1.145AsnGln: 1.145 ± 0.039	1.818AsnArg: 1.818 ± 0.051	2.54AsnSer: 2.54 ± 0.072	2.437AsnThr: 2.437 ± 0.062	3.534AsnVal: 3.534 ± 0.07	0.447AsnTrp: 0.447 ± 0.026	2.152AsnTyr: 2.152 ± 0.057	0.0AsnXaa: 0.0 ± 0.0
Pro	1.929ProAla: 1.929 ± 0.057	0.263ProCys: 0.263 ± 0.022	1.99ProAsp: 1.99 ± 0.054	2.902ProGlu: 2.902 ± 0.07	1.571ProPhe: 1.571 ± 0.054	2.367ProGly: 2.367 ± 0.066	0.727ProHis: 0.727 ± 0.031	2.847ProIle: 2.847 ± 0.06	2.431ProLys: 2.431 ± 0.059	3.225ProLeu: 3.225 ± 0.075	0.784ProMet: 0.784 ± 0.034	1.448ProAsn: 1.448 ± 0.045	1.141ProPro: 1.141 ± 0.05	1.269ProGln: 1.269 ± 0.046	1.055ProArg: 1.055 ± 0.04	1.845ProSer: 1.845 ± 0.05	1.801ProThr: 1.801 ± 0.058	2.897ProVal: 2.897 ± 0.08	0.299ProTrp: 0.299 ± 0.026	1.716ProTyr: 1.716 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	1.615GlnAla: 1.615 ± 0.055	0.152GlnCys: 0.152 ± 0.015	1.172GlnAsp: 1.172 ± 0.04	2.029GlnGlu: 2.029 ± 0.061	0.915GlnPhe: 0.915 ± 0.035	1.467GlnGly: 1.467 ± 0.053	0.355GlnHis: 0.355 ± 0.023	2.358GlnIle: 2.358 ± 0.069	2.664GlnLys: 2.664 ± 0.065	2.235GlnLeu: 2.235 ± 0.062	0.742GlnMet: 0.742 ± 0.037	1.451GlnAsn: 1.451 ± 0.055	0.813GlnPro: 0.813 ± 0.042	1.148GlnGln: 1.148 ± 0.05	1.231GlnArg: 1.231 ± 0.047	1.166GlnSer: 1.166 ± 0.041	1.078GlnThr: 1.078 ± 0.042	1.616GlnVal: 1.616 ± 0.057	0.193GlnTrp: 0.193 ± 0.017	0.986GlnTyr: 0.986 ± 0.048	0.0GlnXaa: 0.0 ± 0.0
Arg	2.22ArgAla: 2.22 ± 0.059	0.334ArgCys: 0.334 ± 0.025	2.021ArgAsp: 2.021 ± 0.055	3.735ArgGlu: 3.735 ± 0.081	1.702ArgPhe: 1.702 ± 0.05	2.694ArgGly: 2.694 ± 0.067	0.579ArgHis: 0.579 ± 0.027	3.749ArgIle: 3.749 ± 0.075	3.329ArgLys: 3.329 ± 0.071	3.702ArgLeu: 3.702 ± 0.084	1.172ArgMet: 1.172 ± 0.043	1.892ArgAsn: 1.892 ± 0.047	1.285ArgPro: 1.285 ± 0.045	1.169ArgGln: 1.169 ± 0.048	1.886ArgArg: 1.886 ± 0.06	1.678ArgSer: 1.678 ± 0.044	1.64ArgThr: 1.64 ± 0.05	2.721ArgVal: 2.721 ± 0.066	0.278ArgTrp: 0.278 ± 0.022	1.782ArgTyr: 1.782 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	2.972SerAla: 2.972 ± 0.067	0.445SerCys: 0.445 ± 0.026	2.546SerAsp: 2.546 ± 0.076	3.325SerGlu: 3.325 ± 0.076	2.697SerPhe: 2.697 ± 0.071	3.662SerGly: 3.662 ± 0.086	0.92SerHis: 0.92 ± 0.04	4.911SerIle: 4.911 ± 0.084	4.447SerLys: 4.447 ± 0.087	5.364SerLeu: 5.364 ± 0.106	1.344SerMet: 1.344 ± 0.047	2.504SerAsn: 2.504 ± 0.065	1.916SerPro: 1.916 ± 0.058	1.788SerGln: 1.788 ± 0.061	2.142SerArg: 2.142 ± 0.064	3.022SerSer: 3.022 ± 0.074	2.62SerThr: 2.62 ± 0.073	3.508SerVal: 3.508 ± 0.091	0.399SerTrp: 0.399 ± 0.025	2.188SerTyr: 2.188 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	3.203ThrAla: 3.203 ± 0.07	0.325ThrCys: 0.325 ± 0.023	2.346ThrAsp: 2.346 ± 0.066	2.996ThrGlu: 2.996 ± 0.063	2.107ThrPhe: 2.107 ± 0.067	3.911ThrGly: 3.911 ± 0.082	0.778ThrHis: 0.778 ± 0.034	4.307ThrIle: 4.307 ± 0.095	3.418ThrLys: 3.418 ± 0.082	4.61ThrLeu: 4.61 ± 0.083	1.052ThrMet: 1.052 ± 0.038	2.121ThrAsn: 2.121 ± 0.064	2.175ThrPro: 2.175 ± 0.068	1.35ThrGln: 1.35 ± 0.045	1.675ThrArg: 1.675 ± 0.054	2.716ThrSer: 2.716 ± 0.07	2.647ThrThr: 2.647 ± 0.09	3.802ThrVal: 3.802 ± 0.084	0.299ThrTrp: 0.299 ± 0.023	1.777ThrTyr: 1.777 ± 0.055	0.0ThrXaa: 0.0 ± 0.0
Val	4.727ValAla: 4.727 ± 0.096	0.652ValCys: 0.652 ± 0.033	4.166ValAsp: 4.166 ± 0.078	5.579ValGlu: 5.579 ± 0.103	3.073ValPhe: 3.073 ± 0.087	4.702ValGly: 4.702 ± 0.095	0.96ValHis: 0.96 ± 0.037	6.967ValIle: 6.967 ± 0.119	6.265ValLys: 6.265 ± 0.108	6.699ValLeu: 6.699 ± 0.12	1.82ValMet: 1.82 ± 0.045	3.377ValAsn: 3.377 ± 0.076	2.531ValPro: 2.531 ± 0.068	1.537ValGln: 1.537 ± 0.052	2.715ValArg: 2.715 ± 0.058	4.107ValSer: 4.107 ± 0.077	3.669ValThr: 3.669 ± 0.084	5.553ValVal: 5.553 ± 0.103	0.43ValTrp: 0.43 ± 0.027	2.843ValTyr: 2.843 ± 0.077	0.0ValXaa: 0.0 ± 0.0
Trp	0.486TrpAla: 0.486 ± 0.028	0.066TrpCys: 0.066 ± 0.01	0.461TrpAsp: 0.461 ± 0.023	0.563TrpGlu: 0.563 ± 0.031	0.307TrpPhe: 0.307 ± 0.023	0.543TrpGly: 0.543 ± 0.032	0.126TrpHis: 0.126 ± 0.013	0.59TrpIle: 0.59 ± 0.033	0.518TrpLys: 0.518 ± 0.027	0.656TrpLeu: 0.656 ± 0.033	0.224TrpMet: 0.224 ± 0.019	0.336TrpAsn: 0.336 ± 0.021	0.238TrpPro: 0.238 ± 0.021	0.271TrpGln: 0.271 ± 0.021	0.351TrpArg: 0.351 ± 0.023	0.352TrpSer: 0.352 ± 0.02	0.315TrpThr: 0.315 ± 0.021	0.462TrpVal: 0.462 ± 0.025	0.098TrpTrp: 0.098 ± 0.012	0.284TrpTyr: 0.284 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.279TyrAla: 2.279 ± 0.057	0.334TyrCys: 0.334 ± 0.024	2.36TyrAsp: 2.36 ± 0.064	2.807TyrGlu: 2.807 ± 0.062	1.944TyrPhe: 1.944 ± 0.055	2.917TyrGly: 2.917 ± 0.071	0.656TyrHis: 0.656 ± 0.029	3.799TyrIle: 3.799 ± 0.088	3.412TyrLys: 3.412 ± 0.07	3.818TyrLeu: 3.818 ± 0.078	0.915TyrMet: 0.915 ± 0.035	2.173TyrAsn: 2.173 ± 0.062	1.472TyrPro: 1.472 ± 0.047	1.093TyrGln: 1.093 ± 0.044	1.567TyrArg: 1.567 ± 0.048	2.173TyrSer: 2.173 ± 0.07	2.068TyrThr: 2.068 ± 0.06	2.682TyrVal: 2.682 ± 0.075	0.325TyrTrp: 0.325 ± 0.02	1.701TyrTyr: 1.701 ± 0.064	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2193 proteins (664459 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---