Amino acid dipepetide frequency for Planctomycetes bacterium MalM25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.933AlaAla: 14.933 ± 0.157	1.268AlaCys: 1.268 ± 0.035	7.273AlaAsp: 7.273 ± 0.078	8.434AlaGlu: 8.434 ± 0.102	3.669AlaPhe: 3.669 ± 0.054	10.027AlaGly: 10.027 ± 0.102	1.723AlaHis: 1.723 ± 0.038	5.483AlaIle: 5.483 ± 0.069	3.65AlaLys: 3.65 ± 0.067	10.25AlaLeu: 10.25 ± 0.101	2.553AlaMet: 2.553 ± 0.053	2.878AlaAsn: 2.878 ± 0.054	5.975AlaPro: 5.975 ± 0.083	3.202AlaGln: 3.202 ± 0.052	6.266AlaArg: 6.266 ± 0.087	6.953AlaSer: 6.953 ± 0.079	6.855AlaThr: 6.855 ± 0.082	7.946AlaVal: 7.946 ± 0.082	1.809AlaTrp: 1.809 ± 0.041	2.523AlaTyr: 2.523 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.75CysAla: 0.75 ± 0.024	0.244CysCys: 0.244 ± 0.022	0.691CysAsp: 0.691 ± 0.023	0.696CysGlu: 0.696 ± 0.028	0.321CysPhe: 0.321 ± 0.019	1.068CysGly: 1.068 ± 0.041	0.34CysHis: 0.34 ± 0.02	0.231CysIle: 0.231 ± 0.012	0.236CysLys: 0.236 ± 0.017	1.103CysLeu: 1.103 ± 0.028	0.128CysMet: 0.128 ± 0.01	0.24CysAsn: 0.24 ± 0.013	0.601CysPro: 0.601 ± 0.025	0.345CysGln: 0.345 ± 0.017	0.783CysArg: 0.783 ± 0.026	0.495CysSer: 0.495 ± 0.02	0.432CysThr: 0.432 ± 0.019	0.793CysVal: 0.793 ± 0.027	0.146CysTrp: 0.146 ± 0.011	0.269CysTyr: 0.269 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	7.321AspAla: 7.321 ± 0.081	0.573AspCys: 0.573 ± 0.022	4.269AspAsp: 4.269 ± 0.073	4.582AspGlu: 4.582 ± 0.065	2.419AspPhe: 2.419 ± 0.056	6.055AspGly: 6.055 ± 0.108	1.378AspHis: 1.378 ± 0.035	1.917AspIle: 1.917 ± 0.041	1.581AspLys: 1.581 ± 0.035	6.67AspLeu: 6.67 ± 0.075	0.845AspMet: 0.845 ± 0.024	1.708AspAsn: 1.708 ± 0.045	4.216AspPro: 4.216 ± 0.067	2.175AspGln: 2.175 ± 0.037	4.825AspArg: 4.825 ± 0.062	3.292AspSer: 3.292 ± 0.066	2.994AspThr: 2.994 ± 0.051	3.591AspVal: 3.591 ± 0.058	1.229AspTrp: 1.229 ± 0.03	1.971AspTyr: 1.971 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	8.268GluAla: 8.268 ± 0.088	0.506GluCys: 0.506 ± 0.022	3.095GluAsp: 3.095 ± 0.05	4.679GluGlu: 4.679 ± 0.084	2.074GluPhe: 2.074 ± 0.042	5.437GluGly: 5.437 ± 0.066	1.471GluHis: 1.471 ± 0.038	2.877GluIle: 2.877 ± 0.047	2.14GluLys: 2.14 ± 0.05	6.988GluLeu: 6.988 ± 0.077	1.38GluMet: 1.38 ± 0.035	1.616GluAsn: 1.616 ± 0.035	4.108GluPro: 4.108 ± 0.063	2.701GluGln: 2.701 ± 0.049	5.292GluArg: 5.292 ± 0.064	4.091GluSer: 4.091 ± 0.066	3.683GluThr: 3.683 ± 0.056	4.703GluVal: 4.703 ± 0.056	0.98GluTrp: 0.98 ± 0.03	1.491GluTyr: 1.491 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	3.753PheAla: 3.753 ± 0.054	0.388PheCys: 0.388 ± 0.017	2.977PheAsp: 2.977 ± 0.057	2.338PheGlu: 2.338 ± 0.04	1.265PhePhe: 1.265 ± 0.038	3.305PheGly: 3.305 ± 0.056	0.622PheHis: 0.622 ± 0.023	1.116PheIle: 1.116 ± 0.032	0.842PheLys: 0.842 ± 0.024	2.896PheLeu: 2.896 ± 0.056	0.536PheMet: 0.536 ± 0.02	1.166PheAsn: 1.166 ± 0.035	1.397PhePro: 1.397 ± 0.034	0.963PheGln: 0.963 ± 0.029	2.097PheArg: 2.097 ± 0.035	1.935PheSer: 1.935 ± 0.043	2.012PheThr: 2.012 ± 0.047	2.502PheVal: 2.502 ± 0.047	0.519PheTrp: 0.519 ± 0.02	0.918PheTyr: 0.918 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	8.769GlyAla: 8.769 ± 0.094	1.021GlyCys: 1.021 ± 0.038	6.084GlyAsp: 6.084 ± 0.097	6.548GlyGlu: 6.548 ± 0.084	3.22GlyPhe: 3.22 ± 0.046	8.429GlyGly: 8.429 ± 0.141	1.612GlyHis: 1.612 ± 0.034	2.757GlyIle: 2.757 ± 0.054	3.039GlyLys: 3.039 ± 0.052	8.164GlyLeu: 8.164 ± 0.083	1.899GlyMet: 1.899 ± 0.041	2.001GlyAsn: 2.001 ± 0.05	3.987GlyPro: 3.987 ± 0.058	2.934GlyGln: 2.934 ± 0.057	5.742GlyArg: 5.742 ± 0.068	5.373GlySer: 5.373 ± 0.09	3.815GlyThr: 3.815 ± 0.064	8.082GlyVal: 8.082 ± 0.082	1.617GlyTrp: 1.617 ± 0.037	2.361GlyTyr: 2.361 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.225HisAla: 2.225 ± 0.048	0.24HisCys: 0.24 ± 0.013	1.244HisAsp: 1.244 ± 0.034	1.301HisGlu: 1.301 ± 0.032	0.791HisPhe: 0.791 ± 0.022	1.832HisGly: 1.832 ± 0.046	0.609HisHis: 0.609 ± 0.024	0.53HisIle: 0.53 ± 0.022	0.488HisLys: 0.488 ± 0.019	1.98HisLeu: 1.98 ± 0.043	0.26HisMet: 0.26 ± 0.014	0.587HisAsn: 0.587 ± 0.023	1.279HisPro: 1.279 ± 0.037	0.681HisGln: 0.681 ± 0.021	1.525HisArg: 1.525 ± 0.037	1.018HisSer: 1.018 ± 0.032	1.062HisThr: 1.062 ± 0.028	1.374HisVal: 1.374 ± 0.03	0.341HisTrp: 0.341 ± 0.018	0.607HisTyr: 0.607 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	5.298IleAla: 5.298 ± 0.07	0.369IleCys: 0.369 ± 0.018	4.181IleAsp: 4.181 ± 0.061	3.597IleGlu: 3.597 ± 0.052	0.937IlePhe: 0.937 ± 0.031	3.962IleGly: 3.962 ± 0.061	0.776IleHis: 0.776 ± 0.027	1.088IleIle: 1.088 ± 0.031	1.032IleLys: 1.032 ± 0.03	2.833IleLeu: 2.833 ± 0.044	0.46IleMet: 0.46 ± 0.019	1.327IleAsn: 1.327 ± 0.036	1.831IlePro: 1.831 ± 0.041	1.108IleGln: 1.108 ± 0.025	2.498IleArg: 2.498 ± 0.046	2.064IleSer: 2.064 ± 0.042	2.318IleThr: 2.318 ± 0.051	3.005IleVal: 3.005 ± 0.049	0.392IleTrp: 0.392 ± 0.016	0.867IleTyr: 0.867 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	2.885LysAla: 2.885 ± 0.059	0.209LysCys: 0.209 ± 0.014	1.231LysAsp: 1.231 ± 0.034	1.759LysGlu: 1.759 ± 0.046	0.783LysPhe: 0.783 ± 0.024	1.956LysGly: 1.956 ± 0.04	0.728LysHis: 0.728 ± 0.027	1.252LysIle: 1.252 ± 0.03	1.485LysLys: 1.485 ± 0.066	3.253LysLeu: 3.253 ± 0.054	0.612LysMet: 0.612 ± 0.023	0.842LysAsn: 0.842 ± 0.022	2.227LysPro: 2.227 ± 0.049	1.316LysGln: 1.316 ± 0.033	2.457LysArg: 2.457 ± 0.044	1.637LysSer: 1.637 ± 0.038	1.753LysThr: 1.753 ± 0.039	1.949LysVal: 1.949 ± 0.041	0.411LysTrp: 0.411 ± 0.016	0.719LysTyr: 0.719 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	13.55LeuAla: 13.55 ± 0.122	1.01LeuCys: 1.01 ± 0.03	6.309LeuAsp: 6.309 ± 0.067	5.798LeuGlu: 5.798 ± 0.06	3.152LeuPhe: 3.152 ± 0.063	8.623LeuGly: 8.623 ± 0.089	1.774LeuHis: 1.774 ± 0.036	4.123LeuIle: 4.123 ± 0.061	2.9LeuLys: 2.9 ± 0.053	10.543LeuLeu: 10.543 ± 0.145	1.929LeuMet: 1.929 ± 0.039	2.604LeuAsn: 2.604 ± 0.046	5.507LeuPro: 5.507 ± 0.076	2.766LeuGln: 2.766 ± 0.046	7.102LeuArg: 7.102 ± 0.091	5.968LeuSer: 5.968 ± 0.068	5.943LeuThr: 5.943 ± 0.066	7.682LeuVal: 7.682 ± 0.092	1.347LeuTrp: 1.347 ± 0.037	2.048LeuTyr: 2.048 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	2.122MetAla: 2.122 ± 0.036	0.131MetCys: 0.131 ± 0.01	0.792MetAsp: 0.792 ± 0.023	0.846MetGlu: 0.846 ± 0.028	0.499MetPhe: 0.499 ± 0.021	1.325MetGly: 1.325 ± 0.036	0.469MetHis: 0.469 ± 0.021	0.932MetIle: 0.932 ± 0.026	0.775MetLys: 0.775 ± 0.024	2.088MetLeu: 2.088 ± 0.045	0.386MetMet: 0.386 ± 0.019	0.63MetAsn: 0.63 ± 0.021	1.307MetPro: 1.307 ± 0.035	0.732MetGln: 0.732 ± 0.023	1.432MetArg: 1.432 ± 0.035	1.313MetSer: 1.313 ± 0.034	1.286MetThr: 1.286 ± 0.031	1.21MetVal: 1.21 ± 0.038	0.198MetTrp: 0.198 ± 0.014	0.34MetTyr: 0.34 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.938AsnAla: 2.938 ± 0.046	0.267AsnCys: 0.267 ± 0.013	1.859AsnAsp: 1.859 ± 0.05	1.685AsnGlu: 1.685 ± 0.037	0.922AsnPhe: 0.922 ± 0.032	2.711AsnGly: 2.711 ± 0.066	0.595AsnHis: 0.595 ± 0.022	0.81AsnIle: 0.81 ± 0.028	0.627AsnLys: 0.627 ± 0.02	2.674AsnLeu: 2.674 ± 0.047	0.367AsnMet: 0.367 ± 0.017	1.024AsnAsn: 1.024 ± 0.04	1.909AsnPro: 1.909 ± 0.037	1.023AsnGln: 1.023 ± 0.035	2.006AsnArg: 2.006 ± 0.038	1.49AsnSer: 1.49 ± 0.039	1.465AsnThr: 1.465 ± 0.038	1.863AsnVal: 1.863 ± 0.04	0.453AsnTrp: 0.453 ± 0.019	0.861AsnTyr: 0.861 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	6.697ProAla: 6.697 ± 0.086	0.414ProCys: 0.414 ± 0.018	3.538ProAsp: 3.538 ± 0.054	4.594ProGlu: 4.594 ± 0.066	1.816ProPhe: 1.816 ± 0.04	4.918ProGly: 4.918 ± 0.067	1.132ProHis: 1.132 ± 0.029	2.617ProIle: 2.617 ± 0.049	1.666ProLys: 1.666 ± 0.037	5.047ProLeu: 5.047 ± 0.074	1.039ProMet: 1.039 ± 0.026	1.77ProAsn: 1.77 ± 0.047	3.402ProPro: 3.402 ± 0.068	1.759ProGln: 1.759 ± 0.043	3.286ProArg: 3.286 ± 0.056	3.57ProSer: 3.57 ± 0.059	3.678ProThr: 3.678 ± 0.053	3.812ProVal: 3.812 ± 0.064	0.784ProTrp: 0.784 ± 0.028	1.234ProTyr: 1.234 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	4.009GlnAla: 4.009 ± 0.064	0.261GlnCys: 0.261 ± 0.014	1.402GlnAsp: 1.402 ± 0.033	1.777GlnGlu: 1.777 ± 0.036	1.039GlnPhe: 1.039 ± 0.027	2.327GlnGly: 2.327 ± 0.045	0.74GlnHis: 0.74 ± 0.026	1.66GlnIle: 1.66 ± 0.031	0.945GlnLys: 0.945 ± 0.025	3.613GlnLeu: 3.613 ± 0.056	0.743GlnMet: 0.743 ± 0.024	0.824GlnAsn: 0.824 ± 0.028	2.083GlnPro: 2.083 ± 0.045	1.602GlnGln: 1.602 ± 0.044	2.656GlnArg: 2.656 ± 0.06	1.901GlnSer: 1.901 ± 0.04	1.969GlnThr: 1.969 ± 0.035	2.33GlnVal: 2.33 ± 0.045	0.487GlnTrp: 0.487 ± 0.021	0.707GlnTyr: 0.707 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	5.996ArgAla: 5.996 ± 0.066	0.695ArgCys: 0.695 ± 0.025	4.115ArgAsp: 4.115 ± 0.056	4.45ArgGlu: 4.45 ± 0.064	2.818ArgPhe: 2.818 ± 0.046	5.142ArgGly: 5.142 ± 0.07	1.428ArgHis: 1.428 ± 0.032	2.9ArgIle: 2.9 ± 0.045	1.936ArgLys: 1.936 ± 0.046	8.717ArgLeu: 8.717 ± 0.106	1.646ArgMet: 1.646 ± 0.033	1.678ArgAsn: 1.678 ± 0.034	3.647ArgPro: 3.647 ± 0.061	2.582ArgGln: 2.582 ± 0.053	6.252ArgArg: 6.252 ± 0.073	3.765ArgSer: 3.765 ± 0.057	3.394ArgThr: 3.394 ± 0.052	5.693ArgVal: 5.693 ± 0.065	1.278ArgTrp: 1.278 ± 0.033	1.97ArgTyr: 1.97 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	5.734SerAla: 5.734 ± 0.068	0.522SerCys: 0.522 ± 0.02	3.668SerAsp: 3.668 ± 0.058	3.622SerGlu: 3.622 ± 0.057	1.987SerPhe: 1.987 ± 0.044	5.712SerGly: 5.712 ± 0.082	1.226SerHis: 1.226 ± 0.033	2.473SerIle: 2.473 ± 0.044	1.638SerLys: 1.638 ± 0.041	6.122SerLeu: 6.122 ± 0.075	1.047SerMet: 1.047 ± 0.029	1.652SerAsn: 1.652 ± 0.044	3.607SerPro: 3.607 ± 0.058	2.038SerGln: 2.038 ± 0.044	3.844SerArg: 3.844 ± 0.053	3.499SerSer: 3.499 ± 0.071	3.269SerThr: 3.269 ± 0.06	4.102SerVal: 4.102 ± 0.058	0.861SerTrp: 0.861 ± 0.025	1.361SerTyr: 1.361 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.936ThrAla: 5.936 ± 0.068	0.49ThrCys: 0.49 ± 0.022	3.382ThrAsp: 3.382 ± 0.058	3.168ThrGlu: 3.168 ± 0.054	1.87ThrPhe: 1.87 ± 0.038	5.188ThrGly: 5.188 ± 0.075	1.116ThrHis: 1.116 ± 0.032	2.683ThrIle: 2.683 ± 0.053	1.483ThrLys: 1.483 ± 0.035	6.208ThrLeu: 6.208 ± 0.076	0.92ThrMet: 0.92 ± 0.028	1.572ThrAsn: 1.572 ± 0.041	4.493ThrPro: 4.493 ± 0.063	1.627ThrGln: 1.627 ± 0.035	3.291ThrArg: 3.291 ± 0.048	2.994ThrSer: 2.994 ± 0.051	3.405ThrThr: 3.405 ± 0.061	4.272ThrVal: 4.272 ± 0.061	0.738ThrTrp: 0.738 ± 0.021	1.265ThrTyr: 1.265 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	8.646ValAla: 8.646 ± 0.089	0.818ValCys: 0.818 ± 0.031	4.726ValAsp: 4.726 ± 0.069	5.29ValGlu: 5.29 ± 0.069	2.464ValPhe: 2.464 ± 0.047	6.04ValGly: 6.04 ± 0.068	1.317ValHis: 1.317 ± 0.031	3.212ValIle: 3.212 ± 0.056	1.893ValLys: 1.893 ± 0.045	7.214ValLeu: 7.214 ± 0.081	1.407ValMet: 1.407 ± 0.036	2.112ValAsn: 2.112 ± 0.045	3.293ValPro: 3.293 ± 0.052	2.054ValGln: 2.054 ± 0.04	5.11ValArg: 5.11 ± 0.068	4.285ValSer: 4.285 ± 0.052	4.599ValThr: 4.599 ± 0.067	6.232ValVal: 6.232 ± 0.075	1.225ValTrp: 1.225 ± 0.032	1.809ValTyr: 1.809 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.372TrpAla: 1.372 ± 0.032	0.179TrpCys: 0.179 ± 0.012	0.917TrpAsp: 0.917 ± 0.025	0.768TrpGlu: 0.768 ± 0.027	0.596TrpPhe: 0.596 ± 0.023	1.075TrpGly: 1.075 ± 0.029	0.334TrpHis: 0.334 ± 0.014	0.687TrpIle: 0.687 ± 0.024	0.518TrpLys: 0.518 ± 0.019	1.84TrpLeu: 1.84 ± 0.047	0.35TrpMet: 0.35 ± 0.018	0.519TrpAsn: 0.519 ± 0.023	0.787TrpPro: 0.787 ± 0.031	0.515TrpGln: 0.515 ± 0.022	1.637TrpArg: 1.637 ± 0.035	1.033TrpSer: 1.033 ± 0.029	0.801TrpThr: 0.801 ± 0.023	0.969TrpVal: 0.969 ± 0.023	0.339TrpTrp: 0.339 ± 0.018	0.339TrpTyr: 0.339 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.469TyrAla: 2.469 ± 0.043	0.309TyrCys: 0.309 ± 0.015	1.859TyrAsp: 1.859 ± 0.042	1.648TyrGlu: 1.648 ± 0.039	0.907TyrPhe: 0.907 ± 0.029	2.323TyrGly: 2.323 ± 0.043	0.585TyrHis: 0.585 ± 0.019	0.595TyrIle: 0.595 ± 0.024	0.59TyrLys: 0.59 ± 0.025	2.478TyrLeu: 2.478 ± 0.048	0.304TyrMet: 0.304 ± 0.014	0.798TyrAsn: 0.798 ± 0.026	1.113TyrPro: 1.113 ± 0.026	0.954TyrGln: 0.954 ± 0.026	2.001TyrArg: 2.001 ± 0.042	1.305TyrSer: 1.305 ± 0.033	1.367TyrThr: 1.367 ± 0.037	1.636TyrVal: 1.636 ± 0.041	0.42TyrTrp: 0.42 ± 0.019	0.775TyrTyr: 0.775 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3738 proteins (1380535 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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