Kazachstania saulgeensis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kazachstania

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5290 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7R5X9|A0A1X7R5X9_9SACH Mediator complex subunit 12 OS=Kazachstania saulgeensis OX=1789683 GN=KASA_0M00275G PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 10.5LSTVVVLLGSSSLALAALSAEE23 pKa = 4.48DD24 pKa = 3.9LAEE27 pKa = 3.99LTVIFSDD34 pKa = 3.38INKK37 pKa = 10.14NGDD40 pKa = 3.47SYY42 pKa = 11.22TNWILDD48 pKa = 3.57SGIDD52 pKa = 3.49MPMDD56 pKa = 3.6VLPILMGVEE65 pKa = 4.13TATDD69 pKa = 3.42SSYY72 pKa = 8.1TTYY75 pKa = 11.35LDD77 pKa = 3.46QLNDD81 pKa = 3.56ADD83 pKa = 3.83VSAFEE88 pKa = 4.61GVATSVPWYY97 pKa = 9.09TSYY100 pKa = 11.23LSSEE104 pKa = 4.06IAVAVANANAEE115 pKa = 4.28AEE117 pKa = 4.52TTAATSAATSSAVDD131 pKa = 3.53SSSATSSVVEE141 pKa = 4.25SSAASAAASSNAATSSAVEE160 pKa = 4.11SSAAASSNAATSSVVEE176 pKa = 4.26SSAAASSNAATAEE189 pKa = 4.13SSAATSSVASSAVAEE204 pKa = 4.38SSAASAAASSNAATSASAATDD225 pKa = 3.19ASTASSAAATDD236 pKa = 3.95ASAATSATDD245 pKa = 3.42ATSVAQASSSAASNQVSSVASSVIASSAASSNITASQEE283 pKa = 4.33TVTNTLTDD291 pKa = 3.37ISSEE295 pKa = 4.17TTTFCPEE302 pKa = 4.18SGDD305 pKa = 3.78VTATAYY311 pKa = 10.56ANSTKK316 pKa = 10.2TVTVCDD322 pKa = 4.3DD323 pKa = 3.21ACKK326 pKa = 10.28SRR328 pKa = 11.84KK329 pKa = 9.2AAEE332 pKa = 3.89ATTTINSLTTATVTKK347 pKa = 10.44CDD349 pKa = 4.03EE350 pKa = 4.23ACHH353 pKa = 5.96ASKK356 pKa = 10.67SAASAASAAAATVTNTQITTATITSCDD383 pKa = 3.87GGCHH387 pKa = 6.11ASKK390 pKa = 10.72SSASAASVASAASAASAASVAKK412 pKa = 9.94EE413 pKa = 3.73NGVAGSASTTVQVQVQTSTTSTQSIAVQNANNAIKK448 pKa = 10.44QSAGLAGLLGAVAIFLLL465 pKa = 4.37

Molecular weight: 44.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7QYF1|A0A1X7QYF1_9SACH Cytoplasmic tRNA 2-thiolation protein 2 OS=Kazachstania saulgeensis OX=1789683 GN=NCS2 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.11WRR6 pKa = 11.84KK7 pKa = 9.07KK8 pKa = 6.93RR9 pKa = 11.84TRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.48RR15 pKa = 11.84KK16 pKa = 8.41RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.19VRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 10.98

Molecular weight: 3.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5290

0

5290

2691636

25

4952

508.8

57.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.913 ± 0.034

1.134 ± 0.012

6.172 ± 0.023

6.499 ± 0.034

4.268 ± 0.023

4.817 ± 0.032

2.027 ± 0.013

7.215 ± 0.031

7.29 ± 0.029

9.242 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.189 ± 0.011

7.067 ± 0.039

4.103 ± 0.022

3.996 ± 0.029

4.117 ± 0.02

8.794 ± 0.041

6.355 ± 0.033

5.418 ± 0.023

0.967 ± 0.01

3.419 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski