Geotrypetes seraphini (Gaboon caecilian) (Caecilia seraphini)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Gymnophiona; Dermophiidae;

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38703 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P8QGM0|A0A6P8QGM0_GEOSA SH3 domain-binding protein 5 OS=Geotrypetes seraphini OX=260995 GN=SH3BP5 PE=3 SV=1
MM1 pKa = 8.24DD2 pKa = 4.68FQQLADD8 pKa = 5.58AADD11 pKa = 3.92KK12 pKa = 10.18WCSNTPFEE20 pKa = 5.73LIATEE25 pKa = 3.96EE26 pKa = 4.32TEE28 pKa = 4.33RR29 pKa = 11.84RR30 pKa = 11.84MDD32 pKa = 4.29FYY34 pKa = 11.47ADD36 pKa = 3.74PGVSFYY42 pKa = 10.98VLCPDD47 pKa = 4.64NGCGDD52 pKa = 3.96NFHH55 pKa = 6.3VWSEE59 pKa = 4.48SEE61 pKa = 3.97DD62 pKa = 3.63CLPFLQLAQDD72 pKa = 4.04YY73 pKa = 10.88LSSCGKK79 pKa = 8.56KK80 pKa = 7.85TLHH83 pKa = 6.85EE84 pKa = 4.23ILEE87 pKa = 4.44KK88 pKa = 10.42VLKK91 pKa = 10.3SFRR94 pKa = 11.84PLLGLPDD101 pKa = 3.71VDD103 pKa = 4.94DD104 pKa = 5.38DD105 pKa = 5.61AFEE108 pKa = 4.61EE109 pKa = 4.49YY110 pKa = 10.57NADD113 pKa = 3.67VEE115 pKa = 4.48EE116 pKa = 4.71EE117 pKa = 4.21EE118 pKa = 5.26PEE120 pKa = 4.6ADD122 pKa = 3.57HH123 pKa = 6.41QQMGVSQQQ131 pKa = 2.96

Molecular weight: 14.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P8RM29|A0A6P8RM29_GEOSA Reverse transcriptase OS=Geotrypetes seraphini OX=260995 GN=LOC117363128 PE=3 SV=1
MM1 pKa = 7.57SSHH4 pKa = 6.01KK5 pKa = 9.07TFKK8 pKa = 10.28IKK10 pKa = 10.6RR11 pKa = 11.84FLAKK15 pKa = 9.68KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19038

19665

38703

28253243

31

38067

730.0

81.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.075 ± 0.012

2.192 ± 0.011

5.025 ± 0.009

7.444 ± 0.021

3.615 ± 0.01

5.68 ± 0.017

2.607 ± 0.008

5.089 ± 0.013

6.591 ± 0.019

9.379 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.297 ± 0.007

4.192 ± 0.01

5.406 ± 0.016

4.931 ± 0.02

5.142 ± 0.01

8.769 ± 0.02

5.779 ± 0.023

5.926 ± 0.016

1.095 ± 0.004

2.765 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski