Amino acid dipepetide frequency for Paenibacillus sp. TCA20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.941AlaAla: 7.941 ± 0.108
0.636AlaCys: 0.636 ± 0.023
4.119AlaAsp: 4.119 ± 0.063
5.828AlaGlu: 5.828 ± 0.068
2.98AlaPhe: 2.98 ± 0.045
6.329AlaGly: 6.329 ± 0.076
1.447AlaHis: 1.447 ± 0.031
5.182AlaIle: 5.182 ± 0.062
3.889AlaLys: 3.889 ± 0.058
7.393AlaLeu: 7.393 ± 0.075
2.234AlaMet: 2.234 ± 0.037
2.568AlaAsn: 2.568 ± 0.043
2.566AlaPro: 2.566 ± 0.043
2.576AlaGln: 2.576 ± 0.042
3.231AlaArg: 3.231 ± 0.054
5.052AlaSer: 5.052 ± 0.06
3.313AlaThr: 3.313 ± 0.053
6.029AlaVal: 6.029 ± 0.065
0.885AlaTrp: 0.885 ± 0.024
2.693AlaTyr: 2.693 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.456CysAla: 0.456 ± 0.018
0.094CysCys: 0.094 ± 0.008
0.353CysAsp: 0.353 ± 0.015
0.436CysGlu: 0.436 ± 0.016
0.261CysPhe: 0.261 ± 0.013
0.673CysGly: 0.673 ± 0.02
0.163CysHis: 0.163 ± 0.01
0.531CysIle: 0.531 ± 0.018
0.288CysLys: 0.288 ± 0.014
0.639CysLeu: 0.639 ± 0.022
0.205CysMet: 0.205 ± 0.011
0.227CysAsn: 0.227 ± 0.013
0.306CysPro: 0.306 ± 0.014
0.205CysGln: 0.205 ± 0.013
0.366CysArg: 0.366 ± 0.015
0.523CysSer: 0.523 ± 0.016
0.39CysThr: 0.39 ± 0.017
0.407CysVal: 0.407 ± 0.017
0.076CysTrp: 0.076 ± 0.007
0.246CysTyr: 0.246 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.725AspAla: 3.725 ± 0.055
0.332AspCys: 0.332 ± 0.016
2.397AspAsp: 2.397 ± 0.059
4.249AspGlu: 4.249 ± 0.051
2.054AspPhe: 2.054 ± 0.036
3.869AspGly: 3.869 ± 0.068
1.289AspHis: 1.289 ± 0.03
3.852AspIle: 3.852 ± 0.053
2.687AspLys: 2.687 ± 0.046
5.058AspLeu: 5.058 ± 0.061
1.543AspMet: 1.543 ± 0.032
1.827AspAsn: 1.827 ± 0.04
2.414AspPro: 2.414 ± 0.05
2.299AspGln: 2.299 ± 0.039
2.709AspArg: 2.709 ± 0.043
2.99AspSer: 2.99 ± 0.047
2.813AspThr: 2.813 ± 0.043
3.522AspVal: 3.522 ± 0.041
0.767AspTrp: 0.767 ± 0.023
2.295AspTyr: 2.295 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
6.196GluAla: 6.196 ± 0.071
0.368GluCys: 0.368 ± 0.016
3.796GluAsp: 3.796 ± 0.051
6.561GluGlu: 6.561 ± 0.074
2.382GluPhe: 2.382 ± 0.044
4.864GluGly: 4.864 ± 0.062
1.83GluHis: 1.83 ± 0.034
4.689GluIle: 4.689 ± 0.062
3.926GluLys: 3.926 ± 0.05
7.752GluLeu: 7.752 ± 0.091
2.23GluMet: 2.23 ± 0.04
2.645GluAsn: 2.645 ± 0.039
2.354GluPro: 2.354 ± 0.044
4.056GluGln: 4.056 ± 0.059
3.858GluArg: 3.858 ± 0.054
3.756GluSer: 3.756 ± 0.05
3.446GluThr: 3.446 ± 0.043
5.006GluVal: 5.006 ± 0.06
1.014GluTrp: 1.014 ± 0.027
2.453GluTyr: 2.453 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
2.903PheAla: 2.903 ± 0.047
0.315PheCys: 0.315 ± 0.015
2.24PheAsp: 2.24 ± 0.039
2.564PheGlu: 2.564 ± 0.039
1.693PhePhe: 1.693 ± 0.047
3.018PheGly: 3.018 ± 0.048
0.879PheHis: 0.879 ± 0.021
3.03PheIle: 3.03 ± 0.047
2.008PheLys: 2.008 ± 0.035
3.517PheLeu: 3.517 ± 0.06
1.203PheMet: 1.203 ± 0.029
1.732PheAsn: 1.732 ± 0.041
1.487PhePro: 1.487 ± 0.031
1.349PheGln: 1.349 ± 0.028
1.835PheArg: 1.835 ± 0.034
2.727PheSer: 2.727 ± 0.043
2.477PheThr: 2.477 ± 0.046
2.824PheVal: 2.824 ± 0.045
0.473PheTrp: 0.473 ± 0.017
1.5PheTyr: 1.5 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
5.302GlyAla: 5.302 ± 0.078
0.607GlyCys: 0.607 ± 0.018
3.434GlyAsp: 3.434 ± 0.047
4.772GlyGlu: 4.772 ± 0.054
3.098GlyPhe: 3.098 ± 0.052
5.216GlyGly: 5.216 ± 0.081
1.465GlyHis: 1.465 ± 0.028
5.773GlyIle: 5.773 ± 0.064
4.142GlyLys: 4.142 ± 0.053
6.752GlyLeu: 6.752 ± 0.076
2.384GlyMet: 2.384 ± 0.043
2.771GlyAsn: 2.771 ± 0.056
1.908GlyPro: 1.908 ± 0.037
2.584GlyGln: 2.584 ± 0.048
3.278GlyArg: 3.278 ± 0.049
4.784GlySer: 4.784 ± 0.066
4.484GlyThr: 4.484 ± 0.074
5.163GlyVal: 5.163 ± 0.057
0.961GlyTrp: 0.961 ± 0.024
3.156GlyTyr: 3.156 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
1.57HisAla: 1.57 ± 0.03
0.184HisCys: 0.184 ± 0.012
1.059HisAsp: 1.059 ± 0.028
1.444HisGlu: 1.444 ± 0.029
1.059HisPhe: 1.059 ± 0.025
1.439HisGly: 1.439 ± 0.031
0.706HisHis: 0.706 ± 0.023
1.617HisIle: 1.617 ± 0.031
0.991HisLys: 0.991 ± 0.026
2.152HisLeu: 2.152 ± 0.039
0.659HisMet: 0.659 ± 0.019
0.741HisAsn: 0.741 ± 0.023
1.25HisPro: 1.25 ± 0.027
0.921HisGln: 0.921 ± 0.027
1.133HisArg: 1.133 ± 0.026
1.297HisSer: 1.297 ± 0.032
1.191HisThr: 1.191 ± 0.03
1.462HisVal: 1.462 ± 0.032
0.297HisTrp: 0.297 ± 0.013
0.968HisTyr: 0.968 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
5.718IleAla: 5.718 ± 0.069
0.56IleCys: 0.56 ± 0.017
3.818IleAsp: 3.818 ± 0.052
4.901IleGlu: 4.901 ± 0.063
2.456IlePhe: 2.456 ± 0.043
5.287IleGly: 5.287 ± 0.072
1.695IleHis: 1.695 ± 0.035
4.701IleIle: 4.701 ± 0.063
3.328IleLys: 3.328 ± 0.047
6.227IleLeu: 6.227 ± 0.086
1.849IleMet: 1.849 ± 0.043
2.595IleAsn: 2.595 ± 0.045
3.3IlePro: 3.3 ± 0.046
2.943IleGln: 2.943 ± 0.043
3.699IleArg: 3.699 ± 0.043
4.838IleSer: 4.838 ± 0.06
4.24IleThr: 4.24 ± 0.054
5.105IleVal: 5.105 ± 0.056
0.723IleTrp: 0.723 ± 0.022
2.356IleTyr: 2.356 ± 0.039
0.0IleXaa: 0.0 ± 0.0
Lys
3.811LysAla: 3.811 ± 0.052
0.253LysCys: 0.253 ± 0.013
3.044LysAsp: 3.044 ± 0.046
4.673LysGlu: 4.673 ± 0.06
1.55LysPhe: 1.55 ± 0.034
3.433LysGly: 3.433 ± 0.055
1.243LysHis: 1.243 ± 0.03
3.077LysIle: 3.077 ± 0.047
3.419LysLys: 3.419 ± 0.052
5.252LysLeu: 5.252 ± 0.054
1.664LysMet: 1.664 ± 0.035
2.111LysAsn: 2.111 ± 0.042
2.148LysPro: 2.148 ± 0.037
2.531LysGln: 2.531 ± 0.049
2.747LysArg: 2.747 ± 0.044
3.069LysSer: 3.069 ± 0.045
2.683LysThr: 2.683 ± 0.04
3.572LysVal: 3.572 ± 0.053
0.725LysTrp: 0.725 ± 0.024
1.956LysTyr: 1.956 ± 0.04
0.0LysXaa: 0.0 ± 0.0
Leu
7.46LeuAla: 7.46 ± 0.08
0.715LeuCys: 0.715 ± 0.023
5.172LeuAsp: 5.172 ± 0.061
6.299LeuGlu: 6.299 ± 0.069
4.284LeuPhe: 4.284 ± 0.069
6.65LeuGly: 6.65 ± 0.076
2.17LeuHis: 2.17 ± 0.04
6.872LeuIle: 6.872 ± 0.083
5.125LeuLys: 5.125 ± 0.056
10.168LeuLeu: 10.168 ± 0.121
2.813LeuMet: 2.813 ± 0.043
4.159LeuAsn: 4.159 ± 0.052
4.222LeuPro: 4.222 ± 0.054
3.801LeuGln: 3.801 ± 0.062
4.54LeuArg: 4.54 ± 0.055
7.223LeuSer: 7.223 ± 0.077
5.874LeuThr: 5.874 ± 0.062
5.982LeuVal: 5.982 ± 0.065
0.951LeuTrp: 0.951 ± 0.026
3.26LeuTyr: 3.26 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
2.121MetAla: 2.121 ± 0.033
0.157MetCys: 0.157 ± 0.01
1.666MetAsp: 1.666 ± 0.034
2.132MetGlu: 2.132 ± 0.035
1.055MetPhe: 1.055 ± 0.026
1.832MetGly: 1.832 ± 0.041
0.519MetHis: 0.519 ± 0.019
2.136MetIle: 2.136 ± 0.044
2.142MetLys: 2.142 ± 0.04
3.088MetLeu: 3.088 ± 0.051
0.979MetMet: 0.979 ± 0.03
1.795MetAsn: 1.795 ± 0.034
1.12MetPro: 1.12 ± 0.028
1.098MetGln: 1.098 ± 0.028
1.286MetArg: 1.286 ± 0.028
2.004MetSer: 2.004 ± 0.034
1.827MetThr: 1.827 ± 0.035
1.778MetVal: 1.778 ± 0.033
0.254MetTrp: 0.254 ± 0.012
0.879MetTyr: 0.879 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
2.812AsnAla: 2.812 ± 0.047
0.239AsnCys: 0.239 ± 0.013
1.996AsnAsp: 1.996 ± 0.041
3.07AsnGlu: 3.07 ± 0.042
1.314AsnPhe: 1.314 ± 0.034
3.151AsnGly: 3.151 ± 0.061
0.912AsnHis: 0.912 ± 0.025
2.605AsnIle: 2.605 ± 0.038
2.221AsnLys: 2.221 ± 0.037
3.487AsnLeu: 3.487 ± 0.047
1.179AsnMet: 1.179 ± 0.026
1.788AsnAsn: 1.788 ± 0.042
2.126AsnPro: 2.126 ± 0.041
1.696AsnGln: 1.696 ± 0.031
2.089AsnArg: 2.089 ± 0.034
2.361AsnSer: 2.361 ± 0.04
2.322AsnThr: 2.322 ± 0.039
2.689AsnVal: 2.689 ± 0.046
0.528AsnTrp: 0.528 ± 0.019
1.524AsnTyr: 1.524 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
3.113ProAla: 3.113 ± 0.047
0.199ProCys: 0.199 ± 0.01
2.605ProAsp: 2.605 ± 0.043
3.603ProGlu: 3.603 ± 0.051
1.788ProPhe: 1.788 ± 0.033
2.895ProGly: 2.895 ± 0.047
0.899ProHis: 0.899 ± 0.02
2.483ProIle: 2.483 ± 0.042
1.743ProLys: 1.743 ± 0.034
3.64ProLeu: 3.64 ± 0.051
0.993ProMet: 0.993 ± 0.022
1.574ProAsn: 1.574 ± 0.033
1.18ProPro: 1.18 ± 0.031
1.359ProGln: 1.359 ± 0.026
1.308ProArg: 1.308 ± 0.028
2.588ProSer: 2.588 ± 0.044
1.838ProThr: 1.838 ± 0.037
3.26ProVal: 3.26 ± 0.045
0.462ProTrp: 0.462 ± 0.018
1.627ProTyr: 1.627 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
3.188GlnAla: 3.188 ± 0.057
0.187GlnCys: 0.187 ± 0.012
2.005GlnAsp: 2.005 ± 0.037
3.025GlnGlu: 3.025 ± 0.041
1.562GlnPhe: 1.562 ± 0.03
2.684GlnGly: 2.684 ± 0.041
0.919GlnHis: 0.919 ± 0.025
2.78GlnIle: 2.78 ± 0.04
2.02GlnLys: 2.02 ± 0.043
4.349GlnLeu: 4.349 ± 0.061
1.323GlnMet: 1.323 ± 0.033
1.518GlnAsn: 1.518 ± 0.036
1.39GlnPro: 1.39 ± 0.03
1.95GlnGln: 1.95 ± 0.042
1.819GlnArg: 1.819 ± 0.031
2.332GlnSer: 2.332 ± 0.04
1.982GlnThr: 1.982 ± 0.038
2.645GlnVal: 2.645 ± 0.044
0.452GlnTrp: 0.452 ± 0.017
1.507GlnTyr: 1.507 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
3.094ArgAla: 3.094 ± 0.042
0.299ArgCys: 0.299 ± 0.012
2.333ArgAsp: 2.333 ± 0.038
3.606ArgGlu: 3.606 ± 0.053
2.075ArgPhe: 2.075 ± 0.037
2.823ArgGly: 2.823 ± 0.039
0.984ArgHis: 0.984 ± 0.023
3.674ArgIle: 3.674 ± 0.047
2.827ArgLys: 2.827 ± 0.045
4.824ArgLeu: 4.824 ± 0.065
1.658ArgMet: 1.658 ± 0.038
2.048ArgAsn: 2.048 ± 0.034
1.54ArgPro: 1.54 ± 0.034
1.84ArgGln: 1.84 ± 0.034
2.396ArgArg: 2.396 ± 0.045
3.084ArgSer: 3.084 ± 0.045
2.625ArgThr: 2.625 ± 0.036
3.093ArgVal: 3.093 ± 0.043
0.56ArgTrp: 0.56 ± 0.02
1.884ArgTyr: 1.884 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
4.698SerAla: 4.698 ± 0.07
0.454SerCys: 0.454 ± 0.017
3.236SerAsp: 3.236 ± 0.051
4.384SerGlu: 4.384 ± 0.055
2.932SerPhe: 2.932 ± 0.047
5.352SerGly: 5.352 ± 0.068
1.268SerHis: 1.268 ± 0.032
4.83SerIle: 4.83 ± 0.063
3.4SerLys: 3.4 ± 0.048
6.357SerLeu: 6.357 ± 0.07
1.941SerMet: 1.941 ± 0.034
2.529SerAsn: 2.529 ± 0.052
2.387SerPro: 2.387 ± 0.038
2.187SerGln: 2.187 ± 0.037
3.011SerArg: 3.011 ± 0.05
4.933SerSer: 4.933 ± 0.07
3.498SerThr: 3.498 ± 0.057
4.505SerVal: 4.505 ± 0.057
0.795SerTrp: 0.795 ± 0.023
2.573SerTyr: 2.573 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
4.748ThrAla: 4.748 ± 0.06
0.309ThrCys: 0.309 ± 0.016
3.048ThrAsp: 3.048 ± 0.05
3.705ThrGlu: 3.705 ± 0.051
2.265ThrPhe: 2.265 ± 0.042
4.733ThrGly: 4.733 ± 0.084
1.082ThrHis: 1.082 ± 0.026
3.881ThrIle: 3.881 ± 0.054
2.531ThrLys: 2.531 ± 0.044
5.398ThrLeu: 5.398 ± 0.055
1.428ThrMet: 1.428 ± 0.03
2.106ThrAsn: 2.106 ± 0.038
2.546ThrPro: 2.546 ± 0.048
1.693ThrGln: 1.693 ± 0.031
2.19ThrArg: 2.19 ± 0.035
3.655ThrSer: 3.655 ± 0.052
3.003ThrThr: 3.003 ± 0.05
4.286ThrVal: 4.286 ± 0.068
0.663ThrTrp: 0.663 ± 0.02
2.055ThrTyr: 2.055 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
4.642ValAla: 4.642 ± 0.054
0.554ValCys: 0.554 ± 0.02
3.603ValAsp: 3.603 ± 0.054
4.475ValGlu: 4.475 ± 0.058
2.828ValPhe: 2.828 ± 0.042
4.29ValGly: 4.29 ± 0.062
1.571ValHis: 1.571 ± 0.034
5.253ValIle: 5.253 ± 0.059
3.76ValLys: 3.76 ± 0.056
7.021ValLeu: 7.021 ± 0.075
2.109ValMet: 2.109 ± 0.036
3.045ValAsn: 3.045 ± 0.049
2.95ValPro: 2.95 ± 0.044
2.735ValGln: 2.735 ± 0.046
3.188ValArg: 3.188 ± 0.041
4.824ValSer: 4.824 ± 0.067
4.477ValThr: 4.477 ± 0.065
4.871ValVal: 4.871 ± 0.072
0.76ValTrp: 0.76 ± 0.024
2.501ValTyr: 2.501 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
0.749TrpAla: 0.749 ± 0.02
0.096TrpCys: 0.096 ± 0.007
0.641TrpAsp: 0.641 ± 0.021
0.776TrpGlu: 0.776 ± 0.022
0.514TrpPhe: 0.514 ± 0.018
0.781TrpGly: 0.781 ± 0.024
0.253TrpHis: 0.253 ± 0.013
0.936TrpIle: 0.936 ± 0.024
0.702TrpLys: 0.702 ± 0.02
1.246TrpLeu: 1.246 ± 0.032
0.424TrpMet: 0.424 ± 0.016
0.718TrpAsn: 0.718 ± 0.021
0.313TrpPro: 0.313 ± 0.016
0.449TrpGln: 0.449 ± 0.017
0.506TrpArg: 0.506 ± 0.019
0.831TrpSer: 0.831 ± 0.023
0.678TrpThr: 0.678 ± 0.021
0.747TrpVal: 0.747 ± 0.022
0.158TrpTrp: 0.158 ± 0.01
0.42TrpTyr: 0.42 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.646TyrAla: 2.646 ± 0.036
0.29TyrCys: 0.29 ± 0.014
2.126TyrAsp: 2.126 ± 0.038
2.68TyrGlu: 2.68 ± 0.04
1.624TyrPhe: 1.624 ± 0.034
2.714TyrGly: 2.714 ± 0.041
0.844TyrHis: 0.844 ± 0.023
2.437TyrIle: 2.437 ± 0.047
1.812TyrLys: 1.812 ± 0.037
3.418TyrLeu: 3.418 ± 0.052
1.048TyrMet: 1.048 ± 0.027
1.612TyrAsn: 1.612 ± 0.031
1.648TyrPro: 1.648 ± 0.034
1.365TyrGln: 1.365 ± 0.028
2.079TyrArg: 2.079 ± 0.038
2.403TyrSer: 2.403 ± 0.046
2.165TyrThr: 2.165 ± 0.049
2.506TyrVal: 2.506 ± 0.041
0.438TyrTrp: 0.438 ± 0.015
1.518TyrTyr: 1.518 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5226 proteins (1621612 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski