Tepidanaerobacter syntrophicus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermosediminibacterales; Tepidanaerobacteraceae; Tepidanaerobacter

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2299 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U9HH48|A0A0U9HH48_9FIRM LexA repressor OS=Tepidanaerobacter syntrophicus OX=224999 GN=lexA PE=3 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84DD3 pKa = 2.97VSLKK7 pKa = 10.09VSYY10 pKa = 10.92LKK12 pKa = 11.17GLVDD16 pKa = 4.38GLDD19 pKa = 3.3IEE21 pKa = 4.52DD22 pKa = 4.56AKK24 pKa = 10.1TKK26 pKa = 10.49RR27 pKa = 11.84AITEE31 pKa = 4.33IINVMSDD38 pKa = 2.45IAEE41 pKa = 4.27AVDD44 pKa = 3.69DD45 pKa = 4.93LEE47 pKa = 5.93VMMEE51 pKa = 3.91DD52 pKa = 3.08TQEE55 pKa = 3.93YY56 pKa = 9.03MEE58 pKa = 6.24SIDD61 pKa = 3.79EE62 pKa = 4.4DD63 pKa = 3.84LGEE66 pKa = 4.85LEE68 pKa = 4.37SEE70 pKa = 4.71VYY72 pKa = 10.89GDD74 pKa = 5.24DD75 pKa = 4.17EE76 pKa = 7.09DD77 pKa = 5.62YY78 pKa = 11.6DD79 pKa = 4.97DD80 pKa = 5.91YY81 pKa = 11.93EE82 pKa = 6.24DD83 pKa = 5.54DD84 pKa = 4.03EE85 pKa = 6.46DD86 pKa = 5.72YY87 pKa = 11.57EE88 pKa = 5.11FDD90 pKa = 3.98DD91 pKa = 4.07EE92 pKa = 5.68GFIEE96 pKa = 4.66VDD98 pKa = 3.6CPNCHH103 pKa = 5.28EE104 pKa = 4.39VVYY107 pKa = 10.3IDD109 pKa = 5.04KK110 pKa = 11.13DD111 pKa = 4.04FIEE114 pKa = 4.91DD115 pKa = 3.86NKK117 pKa = 11.26AEE119 pKa = 4.39CPNCNAVIDD128 pKa = 4.11IEE130 pKa = 4.25QQ131 pKa = 3.28

Molecular weight: 15.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U9HDQ0|A0A0U9HDQ0_9FIRM Murein DD-endopeptidase MepM and murein hydrolase activator NlpD OS=Tepidanaerobacter syntrophicus OX=224999 GN=TSYNT_6259 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.25QPNRR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 9.84RR12 pKa = 11.84KK13 pKa = 7.67KK14 pKa = 8.0THH16 pKa = 5.43GFRR19 pKa = 11.84ARR21 pKa = 11.84MLKK24 pKa = 10.24KK25 pKa = 10.23SGRR28 pKa = 11.84NVIKK32 pKa = 10.48RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.53GRR39 pKa = 11.84KK40 pKa = 8.99ALTAA44 pKa = 4.17

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2299

0

2299

722306

29

2082

314.2

35.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.472 ± 0.051

1.129 ± 0.022

5.523 ± 0.04

7.492 ± 0.058

4.081 ± 0.032

7.049 ± 0.046

1.436 ± 0.019

9.044 ± 0.047

7.954 ± 0.047

9.166 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.665 ± 0.024

4.637 ± 0.035

3.813 ± 0.032

2.726 ± 0.025

3.978 ± 0.036

5.786 ± 0.037

5.0 ± 0.031

6.785 ± 0.038

0.723 ± 0.018

3.543 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski