Apis mellifera associated microvirus 59

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTR6|A0A3S8UTR6_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 59 OX=2494790 PE=4 SV=1
MM1 pKa = 7.78EE2 pKa = 4.03KK3 pKa = 10.0TIPRR7 pKa = 11.84VRR9 pKa = 11.84TMFNASQLPTDD20 pKa = 4.05NEE22 pKa = 4.47PGGGRR27 pKa = 11.84SMTVPDD33 pKa = 3.74MAMGMKK39 pKa = 10.05EE40 pKa = 3.14IMARR44 pKa = 11.84YY45 pKa = 6.69TRR47 pKa = 11.84NLPIDD52 pKa = 4.02GVKK55 pKa = 10.22SPQYY59 pKa = 10.11EE60 pKa = 3.96FRR62 pKa = 11.84EE63 pKa = 3.97GDD65 pKa = 3.22EE66 pKa = 4.6DD67 pKa = 4.61YY68 pKa = 11.59EE69 pKa = 4.83GIEE72 pKa = 4.11PEE74 pKa = 5.46KK75 pKa = 10.07MDD77 pKa = 5.45KK78 pKa = 10.93LDD80 pKa = 4.29LLIASHH86 pKa = 5.73EE87 pKa = 4.34AKK89 pKa = 10.74AQMEE93 pKa = 4.44QINEE97 pKa = 3.95EE98 pKa = 4.11LKK100 pKa = 9.82EE101 pKa = 3.8RR102 pKa = 11.84GYY104 pKa = 11.75AKK106 pKa = 10.3VKK108 pKa = 10.76KK109 pKa = 10.05SDD111 pKa = 3.56EE112 pKa = 4.16KK113 pKa = 10.93PKK115 pKa = 10.77EE116 pKa = 4.2GEE118 pKa = 3.82QQ119 pKa = 3.52

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8U5P1|A0A3Q8U5P1_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 59 OX=2494790 PE=3 SV=1
MM1 pKa = 7.72LMAEE5 pKa = 4.72CLTPFKK11 pKa = 10.36RR12 pKa = 11.84NHH14 pKa = 6.89KK15 pKa = 8.39ITGEE19 pKa = 3.99IQHH22 pKa = 6.25FPCGKK27 pKa = 9.87CPNCIKK33 pKa = 10.39RR34 pKa = 11.84RR35 pKa = 11.84VSGWSEE41 pKa = 3.77RR42 pKa = 11.84LRR44 pKa = 11.84QEE46 pKa = 4.06DD47 pKa = 3.74RR48 pKa = 11.84RR49 pKa = 11.84SEE51 pKa = 3.92TAEE54 pKa = 4.51FITLTYY60 pKa = 8.13TTDD63 pKa = 3.22TVPITKK69 pKa = 10.22NGFMSLKK76 pKa = 10.34KK77 pKa = 10.07RR78 pKa = 11.84DD79 pKa = 3.4VQLFLKK85 pKa = 10.32RR86 pKa = 11.84LRR88 pKa = 11.84KK89 pKa = 9.14SHH91 pKa = 5.85EE92 pKa = 4.32IKK94 pKa = 9.82WKK96 pKa = 10.65SGGKK100 pKa = 8.24TYY102 pKa = 10.48ISQPQYY108 pKa = 9.09PIRR111 pKa = 11.84YY112 pKa = 7.21YY113 pKa = 11.0VCGEE117 pKa = 3.96YY118 pKa = 10.55GGKK121 pKa = 7.76TNRR124 pKa = 11.84PHH126 pKa = 5.53YY127 pKa = 10.4HH128 pKa = 6.91IILFNCDD135 pKa = 3.09LRR137 pKa = 11.84KK138 pKa = 10.16VEE140 pKa = 4.81AAWQNGAIHH149 pKa = 6.67SGTVTGASVGYY160 pKa = 6.38TLKK163 pKa = 11.06YY164 pKa = 8.55MSKK167 pKa = 8.86PSKK170 pKa = 10.48IPMHH174 pKa = 6.73RR175 pKa = 11.84NDD177 pKa = 4.06DD178 pKa = 3.76RR179 pKa = 11.84VKK181 pKa = 10.57EE182 pKa = 4.05FSLMSKK188 pKa = 10.79GIGQNYY194 pKa = 5.86ITPAKK199 pKa = 9.37VAWHH203 pKa = 6.38HH204 pKa = 6.7ADD206 pKa = 3.07LTGRR210 pKa = 11.84MYY212 pKa = 10.54MPIEE216 pKa = 4.69DD217 pKa = 3.91GKK219 pKa = 10.51KK220 pKa = 8.5IAMPRR225 pKa = 11.84YY226 pKa = 8.57YY227 pKa = 10.09KK228 pKa = 10.76DD229 pKa = 3.08KK230 pKa = 10.61IYY232 pKa = 10.89TEE234 pKa = 4.27DD235 pKa = 3.16QRR237 pKa = 11.84KK238 pKa = 9.55IIAEE242 pKa = 3.89WHH244 pKa = 5.88KK245 pKa = 10.92VEE247 pKa = 4.57GDD249 pKa = 3.08IRR251 pKa = 11.84SKK253 pKa = 11.67DD254 pKa = 3.43NIQRR258 pKa = 11.84LIKK261 pKa = 10.3EE262 pKa = 4.22YY263 pKa = 10.96GDD265 pKa = 3.23DD266 pKa = 3.9YY267 pKa = 11.7YY268 pKa = 11.77RR269 pKa = 11.84MKK271 pKa = 11.21LEE273 pKa = 4.57GDD275 pKa = 3.43LKK277 pKa = 10.93LFRR280 pKa = 11.84KK281 pKa = 9.03MFADD285 pKa = 3.64SEE287 pKa = 4.31KK288 pKa = 10.64GRR290 pKa = 11.84NVVV293 pKa = 3.11

Molecular weight: 34.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1267

119

512

316.8

35.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.814 ± 1.325

0.789 ± 0.381

6.235 ± 0.223

5.919 ± 1.308

3.552 ± 1.0

6.946 ± 0.435

2.289 ± 0.465

5.998 ± 0.432

6.235 ± 1.916

7.498 ± 0.816

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.315 ± 0.73

5.367 ± 0.761

5.288 ± 0.9

4.972 ± 0.657

6.788 ± 0.318

6.156 ± 0.632

4.815 ± 0.328

4.262 ± 0.223

1.421 ± 0.492

4.341 ± 0.762
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski