Tobacco leaf curl Pusa virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3VQ44|E3VQ44_9GEMI AC4 OS=Tobacco leaf curl Pusa virus OX=905054 GN=AC4 PE=3 SV=1
MM1 pKa = 7.77PEE3 pKa = 4.68IISSLIHH10 pKa = 6.15SAHH13 pKa = 5.48SPKK16 pKa = 10.16RR17 pKa = 11.84KK18 pKa = 9.46HH19 pKa = 5.61FPNYY23 pKa = 6.37EE24 pKa = 4.32TYY26 pKa = 10.59PHH28 pKa = 6.48LQIKK32 pKa = 9.99NSSRR36 pKa = 11.84SAEE39 pKa = 4.06SFTKK43 pKa = 10.51NGSPHH48 pKa = 6.03LHH50 pKa = 5.86VLIQFEE56 pKa = 4.78GKK58 pKa = 9.96YY59 pKa = 9.72KK60 pKa = 10.07CQNNRR65 pKa = 11.84FFDD68 pKa = 4.02LVSPTRR74 pKa = 11.84SAHH77 pKa = 4.86FHH79 pKa = 6.55PNIQGAKK86 pKa = 9.25SSSDD90 pKa = 3.12VKK92 pKa = 11.22SYY94 pKa = 10.05MDD96 pKa = 3.88KK97 pKa = 11.36DD98 pKa = 3.57GDD100 pKa = 3.83TLEE103 pKa = 4.14WGEE106 pKa = 3.84FQIDD110 pKa = 3.21GRR112 pKa = 11.84SARR115 pKa = 11.84GGQQSANDD123 pKa = 4.11AYY125 pKa = 10.85AKK127 pKa = 10.5AINTGSKK134 pKa = 10.25SEE136 pKa = 3.94ALSVIKK142 pKa = 10.3EE143 pKa = 4.21LAPKK147 pKa = 10.3DD148 pKa = 3.74YY149 pKa = 11.2VLQFHH154 pKa = 6.76NLNANLDD161 pKa = 4.25RR162 pKa = 11.84IFAPPLEE169 pKa = 4.54VFVSPFLSSSFDD181 pKa = 3.44QVPEE185 pKa = 3.96EE186 pKa = 4.18LEE188 pKa = 4.15CWVSEE193 pKa = 4.2NVRR196 pKa = 11.84DD197 pKa = 3.6AAARR201 pKa = 11.84PWRR204 pKa = 11.84PVSIVIEE211 pKa = 4.47GEE213 pKa = 4.02SRR215 pKa = 11.84TGKK218 pKa = 8.58TMWARR223 pKa = 11.84SLGPHH228 pKa = 6.71NYY230 pKa = 10.18LCGHH234 pKa = 7.38LDD236 pKa = 4.18LSPKK240 pKa = 10.15VYY242 pKa = 11.07SNDD245 pKa = 2.25AWYY248 pKa = 10.92NVIDD252 pKa = 5.2DD253 pKa = 4.41VDD255 pKa = 3.61PHH257 pKa = 5.9YY258 pKa = 11.01LKK260 pKa = 10.63HH261 pKa = 6.78FKK263 pKa = 10.66DD264 pKa = 3.18SWGPKK269 pKa = 8.23GTGKK273 pKa = 7.96EE274 pKa = 4.12HH275 pKa = 7.16KK276 pKa = 10.23VRR278 pKa = 11.84EE279 pKa = 3.97ALQIKK284 pKa = 8.32WNPTISLNPGPKK296 pKa = 9.49SSIRR300 pKa = 11.84EE301 pKa = 4.09DD302 pKa = 3.6EE303 pKa = 4.48EE304 pKa = 4.48KK305 pKa = 11.15NSALKK310 pKa = 10.55DD311 pKa = 3.36WALKK315 pKa = 9.35NAEE318 pKa = 4.86FITLTEE324 pKa = 3.98PLYY327 pKa = 11.1SGTHH331 pKa = 5.03QSPAQNSQEE340 pKa = 4.24EE341 pKa = 4.63TSSQAA346 pKa = 3.13

Molecular weight: 38.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3VQ41|E3VQ41_9GEMI Replication enhancer OS=Tobacco leaf curl Pusa virus OX=905054 GN=AC3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.52IVISTPASKK16 pKa = 10.29VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 9.79VSRR30 pKa = 11.84AAAPTVLVTNKK41 pKa = 9.31RR42 pKa = 11.84RR43 pKa = 11.84TWVNRR48 pKa = 11.84PMYY51 pKa = 9.32KK52 pKa = 9.87KK53 pKa = 9.36PKK55 pKa = 8.1IYY57 pKa = 10.5RR58 pKa = 11.84MYY60 pKa = 10.61RR61 pKa = 11.84SPDD64 pKa = 3.29VPKK67 pKa = 10.81GCEE70 pKa = 4.39GPCKK74 pKa = 9.35VQSYY78 pKa = 7.02EE79 pKa = 3.75QRR81 pKa = 11.84HH82 pKa = 6.38DD83 pKa = 3.18IAHH86 pKa = 6.1TGKK89 pKa = 10.34VICVSDD95 pKa = 3.47VTRR98 pKa = 11.84GNGLTHH104 pKa = 6.89RR105 pKa = 11.84VGKK108 pKa = 9.73RR109 pKa = 11.84FCVKK113 pKa = 10.32SIYY116 pKa = 10.42VLGKK120 pKa = 9.13IWMDD124 pKa = 3.35EE125 pKa = 4.04NIKK128 pKa = 9.32TKK130 pKa = 10.61NHH132 pKa = 5.92TNTVMFYY139 pKa = 10.33LVRR142 pKa = 11.84DD143 pKa = 3.68RR144 pKa = 11.84RR145 pKa = 11.84PFGTAMDD152 pKa = 4.48FGQVFNMYY160 pKa = 10.66DD161 pKa = 3.86NEE163 pKa = 4.34PSTATVKK170 pKa = 10.85NDD172 pKa = 2.82LRR174 pKa = 11.84DD175 pKa = 3.42RR176 pKa = 11.84FQVLRR181 pKa = 11.84KK182 pKa = 8.38FTATVTGGQYY192 pKa = 10.85ASKK195 pKa = 10.11EE196 pKa = 3.81QALVRR201 pKa = 11.84KK202 pKa = 9.47FMKK205 pKa = 9.77INNYY209 pKa = 8.31VVYY212 pKa = 10.35NHH214 pKa = 6.06QEE216 pKa = 3.36AAKK219 pKa = 10.14YY220 pKa = 10.5DD221 pKa = 3.75NHH223 pKa = 6.5TEE225 pKa = 4.02NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.86ATLKK246 pKa = 9.47IRR248 pKa = 11.84IYY250 pKa = 10.65FYY252 pKa = 11.36DD253 pKa = 3.17SVQNYY258 pKa = 9.55KK259 pKa = 10.51YY260 pKa = 11.05

Molecular weight: 30.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1085

96

346

180.8

20.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.346 ± 0.711

2.212 ± 0.65

4.977 ± 0.313

4.516 ± 0.922

3.779 ± 0.283

4.516 ± 0.352

3.871 ± 0.511

5.253 ± 0.65

6.175 ± 0.837

7.373 ± 0.747

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.12 ± 0.656

5.714 ± 0.284

5.991 ± 0.632

5.161 ± 0.856

6.728 ± 1.046

8.94 ± 1.461

5.714 ± 0.845

5.899 ± 0.925

1.567 ± 0.313

4.147 ± 0.818
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski