Amino acid dipepetide frequency for Synechococcus phage S-CBS4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.023AlaAla: 10.023 ± 1.173
1.56AlaCys: 1.56 ± 0.29
5.579AlaAsp: 5.579 ± 0.471
5.2AlaGlu: 5.2 ± 0.554
3.782AlaPhe: 3.782 ± 0.463
8.321AlaGly: 8.321 ± 0.775
1.229AlaHis: 1.229 ± 0.231
6.004AlaIle: 6.004 ± 0.546
4.633AlaLys: 4.633 ± 0.671
8.699AlaLeu: 8.699 ± 0.776
2.08AlaMet: 2.08 ± 0.374
4.822AlaAsn: 4.822 ± 0.604
3.735AlaPro: 3.735 ± 0.309
4.633AlaGln: 4.633 ± 0.628
4.586AlaArg: 4.586 ± 0.418
7.47AlaSer: 7.47 ± 0.645
7.233AlaThr: 7.233 ± 1.178
6.004AlaVal: 6.004 ± 0.633
1.135AlaTrp: 1.135 ± 0.215
2.553AlaTyr: 2.553 ± 0.403
0.0AlaXaa: 0.0 ± 0.0
Cys
0.709CysAla: 0.709 ± 0.214
0.425CysCys: 0.425 ± 0.116
0.946CysAsp: 0.946 ± 0.215
0.615CysGlu: 0.615 ± 0.189
0.473CysPhe: 0.473 ± 0.175
0.898CysGly: 0.898 ± 0.191
0.473CysHis: 0.473 ± 0.175
0.662CysIle: 0.662 ± 0.186
0.756CysLys: 0.756 ± 0.191
0.756CysLeu: 0.756 ± 0.219
0.189CysMet: 0.189 ± 0.097
0.378CysAsn: 0.378 ± 0.12
0.52CysPro: 0.52 ± 0.197
0.236CysGln: 0.236 ± 0.122
0.709CysArg: 0.709 ± 0.224
0.52CysSer: 0.52 ± 0.147
0.473CysThr: 0.473 ± 0.173
0.52CysVal: 0.52 ± 0.221
0.331CysTrp: 0.331 ± 0.115
0.189CysTyr: 0.189 ± 0.084
0.0CysXaa: 0.0 ± 0.0
Asp
6.335AspAla: 6.335 ± 0.606
0.709AspCys: 0.709 ± 0.205
3.64AspAsp: 3.64 ± 0.391
3.451AspGlu: 3.451 ± 0.338
2.931AspPhe: 2.931 ± 0.418
6.713AspGly: 6.713 ± 0.558
0.756AspHis: 0.756 ± 0.2
3.498AspIle: 3.498 ± 0.393
2.364AspLys: 2.364 ± 0.326
4.775AspLeu: 4.775 ± 0.466
1.324AspMet: 1.324 ± 0.249
2.6AspAsn: 2.6 ± 0.319
2.931AspPro: 2.931 ± 0.343
2.222AspGln: 2.222 ± 0.301
3.404AspArg: 3.404 ± 0.477
4.113AspSer: 4.113 ± 0.508
3.026AspThr: 3.026 ± 0.375
3.593AspVal: 3.593 ± 0.446
0.567AspTrp: 0.567 ± 0.148
1.986AspTyr: 1.986 ± 0.327
0.0AspXaa: 0.0 ± 0.0
Glu
7.186GluAla: 7.186 ± 0.611
0.567GluCys: 0.567 ± 0.153
3.404GluAsp: 3.404 ± 0.393
4.397GluGlu: 4.397 ± 0.551
2.317GluPhe: 2.317 ± 0.277
4.775GluGly: 4.775 ± 0.435
1.276GluHis: 1.276 ± 0.296
3.64GluIle: 3.64 ± 0.406
3.64GluLys: 3.64 ± 0.447
6.241GluLeu: 6.241 ± 0.547
1.655GluMet: 1.655 ± 0.34
1.938GluAsn: 1.938 ± 0.362
1.749GluPro: 1.749 ± 0.303
2.742GluGln: 2.742 ± 0.398
2.978GluArg: 2.978 ± 0.391
3.451GluSer: 3.451 ± 0.339
2.884GluThr: 2.884 ± 0.334
3.215GluVal: 3.215 ± 0.425
0.946GluTrp: 0.946 ± 0.224
2.458GluTyr: 2.458 ± 0.308
0.0GluXaa: 0.0 ± 0.0
Phe
3.12PheAla: 3.12 ± 0.473
0.425PheCys: 0.425 ± 0.163
2.695PheAsp: 2.695 ± 0.33
2.6PheGlu: 2.6 ± 0.351
1.466PhePhe: 1.466 ± 0.285
2.884PheGly: 2.884 ± 0.391
0.331PheHis: 0.331 ± 0.119
1.229PheIle: 1.229 ± 0.238
2.458PheLys: 2.458 ± 0.374
2.127PheLeu: 2.127 ± 0.365
1.182PheMet: 1.182 ± 0.223
2.364PheAsn: 2.364 ± 0.335
1.938PhePro: 1.938 ± 0.29
0.993PheGln: 0.993 ± 0.165
1.797PheArg: 1.797 ± 0.312
2.6PheSer: 2.6 ± 0.357
2.269PheThr: 2.269 ± 0.348
2.978PheVal: 2.978 ± 0.413
0.331PheTrp: 0.331 ± 0.133
1.418PheTyr: 1.418 ± 0.266
0.0PheXaa: 0.0 ± 0.0
Gly
8.557GlyAla: 8.557 ± 0.855
0.615GlyCys: 0.615 ± 0.181
5.579GlyAsp: 5.579 ± 0.504
3.924GlyGlu: 3.924 ± 0.455
3.168GlyPhe: 3.168 ± 0.431
9.361GlyGly: 9.361 ± 2.265
0.804GlyHis: 0.804 ± 0.202
4.586GlyIle: 4.586 ± 0.379
4.87GlyLys: 4.87 ± 0.477
7.233GlyLeu: 7.233 ± 0.615
1.891GlyMet: 1.891 ± 0.291
4.775GlyAsn: 4.775 ± 0.953
3.026GlyPro: 3.026 ± 0.463
3.404GlyGln: 3.404 ± 0.437
4.444GlyArg: 4.444 ± 0.509
6.524GlySer: 6.524 ± 0.88
6.571GlyThr: 6.571 ± 0.922
4.68GlyVal: 4.68 ± 0.442
1.087GlyTrp: 1.087 ± 0.219
2.506GlyTyr: 2.506 ± 0.402
0.0GlyXaa: 0.0 ± 0.0
His
1.276HisAla: 1.276 ± 0.265
0.378HisCys: 0.378 ± 0.145
0.898HisAsp: 0.898 ± 0.215
1.182HisGlu: 1.182 ± 0.263
0.709HisPhe: 0.709 ± 0.198
2.127HisGly: 2.127 ± 0.346
0.567HisHis: 0.567 ± 0.178
0.756HisIle: 0.756 ± 0.188
0.567HisLys: 0.567 ± 0.229
1.276HisLeu: 1.276 ± 0.227
0.142HisMet: 0.142 ± 0.087
0.615HisAsn: 0.615 ± 0.179
0.946HisPro: 0.946 ± 0.234
0.425HisGln: 0.425 ± 0.152
0.709HisArg: 0.709 ± 0.157
0.52HisSer: 0.52 ± 0.185
0.898HisThr: 0.898 ± 0.185
0.662HisVal: 0.662 ± 0.169
0.425HisTrp: 0.425 ± 0.147
0.756HisTyr: 0.756 ± 0.204
0.0HisXaa: 0.0 ± 0.0
Ile
6.241IleAla: 6.241 ± 0.509
0.331IleCys: 0.331 ± 0.138
3.498IleAsp: 3.498 ± 0.425
4.066IleGlu: 4.066 ± 0.51
1.087IlePhe: 1.087 ± 0.216
4.349IleGly: 4.349 ± 0.483
0.851IleHis: 0.851 ± 0.212
2.269IleIle: 2.269 ± 0.367
2.931IleLys: 2.931 ± 0.458
3.971IleLeu: 3.971 ± 0.472
0.804IleMet: 0.804 ± 0.198
2.175IleAsn: 2.175 ± 0.345
2.317IlePro: 2.317 ± 0.372
1.844IleGln: 1.844 ± 0.349
3.215IleArg: 3.215 ± 0.433
5.248IleSer: 5.248 ± 0.46
4.066IleThr: 4.066 ± 0.503
3.309IleVal: 3.309 ± 0.392
0.425IleTrp: 0.425 ± 0.124
1.607IleTyr: 1.607 ± 0.306
0.0IleXaa: 0.0 ± 0.0
Lys
5.579LysAla: 5.579 ± 0.589
0.284LysCys: 0.284 ± 0.126
2.931LysAsp: 2.931 ± 0.469
4.16LysGlu: 4.16 ± 0.56
0.898LysPhe: 0.898 ± 0.217
3.829LysGly: 3.829 ± 0.472
0.993LysHis: 0.993 ± 0.218
2.269LysIle: 2.269 ± 0.345
2.222LysLys: 2.222 ± 0.328
4.302LysLeu: 4.302 ± 0.519
0.946LysMet: 0.946 ± 0.215
2.08LysAsn: 2.08 ± 0.311
1.749LysPro: 1.749 ± 0.279
1.986LysGln: 1.986 ± 0.298
2.931LysArg: 2.931 ± 0.366
3.309LysSer: 3.309 ± 0.412
2.175LysThr: 2.175 ± 0.346
2.837LysVal: 2.837 ± 0.333
0.804LysTrp: 0.804 ± 0.201
1.371LysTyr: 1.371 ± 0.216
0.0LysXaa: 0.0 ± 0.0
Leu
7.47LeuAla: 7.47 ± 0.461
0.473LeuCys: 0.473 ± 0.158
5.579LeuAsp: 5.579 ± 0.456
5.106LeuGlu: 5.106 ± 0.594
2.978LeuPhe: 2.978 ± 0.359
5.248LeuGly: 5.248 ± 0.645
1.229LeuHis: 1.229 ± 0.277
3.782LeuIle: 3.782 ± 0.378
3.404LeuLys: 3.404 ± 0.541
5.673LeuLeu: 5.673 ± 0.556
1.702LeuMet: 1.702 ± 0.259
3.688LeuAsn: 3.688 ± 0.461
5.342LeuPro: 5.342 ± 0.557
3.546LeuGln: 3.546 ± 0.493
4.964LeuArg: 4.964 ± 0.442
6.335LeuSer: 6.335 ± 0.546
4.586LeuThr: 4.586 ± 0.557
4.728LeuVal: 4.728 ± 0.455
1.182LeuTrp: 1.182 ± 0.301
2.695LeuTyr: 2.695 ± 0.393
0.0LeuXaa: 0.0 ± 0.0
Met
3.12MetAla: 3.12 ± 0.331
0.142MetCys: 0.142 ± 0.08
1.607MetAsp: 1.607 ± 0.266
1.324MetGlu: 1.324 ± 0.29
0.756MetPhe: 0.756 ± 0.182
1.418MetGly: 1.418 ± 0.271
0.425MetHis: 0.425 ± 0.144
0.898MetIle: 0.898 ± 0.225
1.466MetLys: 1.466 ± 0.326
1.749MetLeu: 1.749 ± 0.287
0.236MetMet: 0.236 ± 0.119
0.946MetAsn: 0.946 ± 0.222
0.709MetPro: 0.709 ± 0.171
0.756MetGln: 0.756 ± 0.203
1.087MetArg: 1.087 ± 0.232
1.513MetSer: 1.513 ± 0.284
1.607MetThr: 1.607 ± 0.3
1.466MetVal: 1.466 ± 0.244
0.095MetTrp: 0.095 ± 0.071
0.567MetTyr: 0.567 ± 0.153
0.0MetXaa: 0.0 ± 0.0
Asn
5.39AsnAla: 5.39 ± 0.69
0.473AsnCys: 0.473 ± 0.142
3.309AsnAsp: 3.309 ± 0.468
2.364AsnGlu: 2.364 ± 0.341
1.087AsnPhe: 1.087 ± 0.252
6.051AsnGly: 6.051 ± 0.797
0.946AsnHis: 0.946 ± 0.195
2.648AsnIle: 2.648 ± 0.436
1.04AsnLys: 1.04 ± 0.199
2.837AsnLeu: 2.837 ± 0.353
0.946AsnMet: 0.946 ± 0.241
2.364AsnAsn: 2.364 ± 0.381
2.269AsnPro: 2.269 ± 0.365
1.229AsnGln: 1.229 ± 0.238
2.222AsnArg: 2.222 ± 0.407
2.742AsnSer: 2.742 ± 0.502
2.789AsnThr: 2.789 ± 0.495
2.789AsnVal: 2.789 ± 0.345
0.473AsnTrp: 0.473 ± 0.182
1.607AsnTyr: 1.607 ± 0.198
0.0AsnXaa: 0.0 ± 0.0
Pro
3.593ProAla: 3.593 ± 0.409
0.473ProCys: 0.473 ± 0.159
2.742ProAsp: 2.742 ± 0.378
3.168ProGlu: 3.168 ± 0.388
1.655ProPhe: 1.655 ± 0.239
3.168ProGly: 3.168 ± 0.449
0.756ProHis: 0.756 ± 0.177
2.458ProIle: 2.458 ± 0.328
1.513ProLys: 1.513 ± 0.246
3.451ProLeu: 3.451 ± 0.485
0.851ProMet: 0.851 ± 0.197
1.371ProAsn: 1.371 ± 0.191
1.56ProPro: 1.56 ± 0.3
1.466ProGln: 1.466 ± 0.283
1.607ProArg: 1.607 ± 0.296
3.971ProSer: 3.971 ± 0.435
2.648ProThr: 2.648 ± 0.243
3.546ProVal: 3.546 ± 0.385
0.52ProTrp: 0.52 ± 0.138
1.466ProTyr: 1.466 ± 0.239
0.0ProXaa: 0.0 ± 0.0
Gln
4.397GlnAla: 4.397 ± 0.605
0.425GlnCys: 0.425 ± 0.14
2.175GlnAsp: 2.175 ± 0.277
2.411GlnGlu: 2.411 ± 0.276
1.276GlnPhe: 1.276 ± 0.249
3.357GlnGly: 3.357 ± 0.335
0.709GlnHis: 0.709 ± 0.194
2.317GlnIle: 2.317 ± 0.322
1.324GlnLys: 1.324 ± 0.292
2.884GlnLeu: 2.884 ± 0.372
0.804GlnMet: 0.804 ± 0.291
1.324GlnAsn: 1.324 ± 0.254
1.371GlnPro: 1.371 ± 0.331
1.56GlnGln: 1.56 ± 0.337
2.411GlnArg: 2.411 ± 0.327
2.553GlnSer: 2.553 ± 0.365
1.938GlnThr: 1.938 ± 0.292
2.364GlnVal: 2.364 ± 0.419
1.229GlnTrp: 1.229 ± 0.247
1.182GlnTyr: 1.182 ± 0.276
0.0GlnXaa: 0.0 ± 0.0
Arg
4.113ArgAla: 4.113 ± 0.44
0.804ArgCys: 0.804 ± 0.221
2.884ArgAsp: 2.884 ± 0.378
3.451ArgGlu: 3.451 ± 0.369
1.938ArgPhe: 1.938 ± 0.324
3.735ArgGly: 3.735 ± 0.384
1.276ArgHis: 1.276 ± 0.242
3.262ArgIle: 3.262 ± 0.407
3.215ArgLys: 3.215 ± 0.429
4.444ArgLeu: 4.444 ± 0.493
1.466ArgMet: 1.466 ± 0.268
2.175ArgAsn: 2.175 ± 0.255
1.938ArgPro: 1.938 ± 0.227
2.789ArgGln: 2.789 ± 0.33
3.546ArgArg: 3.546 ± 0.492
2.978ArgSer: 2.978 ± 0.378
3.073ArgThr: 3.073 ± 0.34
3.546ArgVal: 3.546 ± 0.409
1.135ArgTrp: 1.135 ± 0.248
1.938ArgTyr: 1.938 ± 0.365
0.0ArgXaa: 0.0 ± 0.0
Ser
6.335SerAla: 6.335 ± 0.837
0.709SerCys: 0.709 ± 0.223
3.593SerAsp: 3.593 ± 0.358
3.451SerGlu: 3.451 ± 0.444
4.019SerPhe: 4.019 ± 0.463
7.99SerGly: 7.99 ± 0.98
0.946SerHis: 0.946 ± 0.219
3.877SerIle: 3.877 ± 0.452
3.026SerLys: 3.026 ± 0.301
6.43SerLeu: 6.43 ± 0.658
1.749SerMet: 1.749 ± 0.302
2.837SerAsn: 2.837 ± 0.436
3.026SerPro: 3.026 ± 0.353
2.364SerGln: 2.364 ± 0.256
3.073SerArg: 3.073 ± 0.383
5.531SerSer: 5.531 ± 0.504
5.342SerThr: 5.342 ± 0.751
4.302SerVal: 4.302 ± 0.417
1.324SerTrp: 1.324 ± 0.265
2.033SerTyr: 2.033 ± 0.313
0.0SerXaa: 0.0 ± 0.0
Thr
6.288ThrAla: 6.288 ± 1.229
0.662ThrCys: 0.662 ± 0.186
2.222ThrAsp: 2.222 ± 0.341
2.931ThrGlu: 2.931 ± 0.43
2.6ThrPhe: 2.6 ± 0.287
5.72ThrGly: 5.72 ± 0.682
0.756ThrHis: 0.756 ± 0.257
5.153ThrIle: 5.153 ± 0.677
2.742ThrLys: 2.742 ± 0.351
4.728ThrLeu: 4.728 ± 0.59
1.229ThrMet: 1.229 ± 0.237
3.357ThrAsn: 3.357 ± 0.553
2.458ThrPro: 2.458 ± 0.307
1.986ThrGln: 1.986 ± 0.281
2.742ThrArg: 2.742 ± 0.423
4.349ThrSer: 4.349 ± 0.651
4.539ThrThr: 4.539 ± 0.735
4.87ThrVal: 4.87 ± 0.581
0.851ThrTrp: 0.851 ± 0.273
1.938ThrTyr: 1.938 ± 0.339
0.0ThrXaa: 0.0 ± 0.0
Val
6.051ValAla: 6.051 ± 0.498
0.615ValCys: 0.615 ± 0.193
3.688ValAsp: 3.688 ± 0.434
4.68ValGlu: 4.68 ± 0.437
2.411ValPhe: 2.411 ± 0.314
4.491ValGly: 4.491 ± 0.559
0.804ValHis: 0.804 ± 0.231
2.978ValIle: 2.978 ± 0.339
2.695ValLys: 2.695 ± 0.315
4.397ValLeu: 4.397 ± 0.476
1.844ValMet: 1.844 ± 0.307
3.168ValAsn: 3.168 ± 0.446
2.789ValPro: 2.789 ± 0.321
2.364ValGln: 2.364 ± 0.379
3.782ValArg: 3.782 ± 0.362
5.295ValSer: 5.295 ± 0.502
4.019ValThr: 4.019 ± 0.524
5.059ValVal: 5.059 ± 0.498
0.425ValTrp: 0.425 ± 0.121
1.938ValTyr: 1.938 ± 0.313
0.0ValXaa: 0.0 ± 0.0
Trp
0.946TrpAla: 0.946 ± 0.169
0.331TrpCys: 0.331 ± 0.122
1.04TrpAsp: 1.04 ± 0.247
0.993TrpGlu: 0.993 ± 0.217
0.284TrpPhe: 0.284 ± 0.109
0.662TrpGly: 0.662 ± 0.179
0.189TrpHis: 0.189 ± 0.106
0.425TrpIle: 0.425 ± 0.151
1.04TrpLys: 1.04 ± 0.244
1.182TrpLeu: 1.182 ± 0.254
0.331TrpMet: 0.331 ± 0.098
0.946TrpAsn: 0.946 ± 0.22
0.52TrpPro: 0.52 ± 0.154
0.425TrpGln: 0.425 ± 0.137
1.229TrpArg: 1.229 ± 0.236
0.662TrpSer: 0.662 ± 0.186
0.946TrpThr: 0.946 ± 0.189
0.851TrpVal: 0.851 ± 0.191
0.189TrpTrp: 0.189 ± 0.105
0.615TrpTyr: 0.615 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.553TyrAla: 2.553 ± 0.314
0.378TyrCys: 0.378 ± 0.152
2.931TyrAsp: 2.931 ± 0.416
1.986TyrGlu: 1.986 ± 0.293
1.371TyrPhe: 1.371 ± 0.26
2.506TyrGly: 2.506 ± 0.382
0.52TyrHis: 0.52 ± 0.176
1.891TyrIle: 1.891 ± 0.298
1.749TyrLys: 1.749 ± 0.259
2.553TyrLeu: 2.553 ± 0.286
0.473TyrMet: 0.473 ± 0.146
1.655TyrAsn: 1.655 ± 0.26
1.087TyrPro: 1.087 ± 0.229
1.04TyrGln: 1.04 ± 0.208
2.269TyrArg: 2.269 ± 0.297
2.317TyrSer: 2.317 ± 0.354
1.04TyrThr: 1.04 ± 0.216
2.175TyrVal: 2.175 ± 0.304
0.331TyrTrp: 0.331 ± 0.141
1.56TyrTyr: 1.56 ± 0.285
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 105 proteins (21153 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski