candidate division MSBL1 archaeon SCGC-AAA259A05

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133U380|A0A133U380_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA259A05 OX=1698259 GN=AKJ57_06550 PE=4 SV=1
MM1 pKa = 8.12DD2 pKa = 4.01ISGNISDD9 pKa = 3.97STEE12 pKa = 3.87QYY14 pKa = 11.1SGVPASNLSLAEE26 pKa = 4.1NKK28 pKa = 10.29SVTASSNEE36 pKa = 3.74NHH38 pKa = 6.03TEE40 pKa = 3.74WYY42 pKa = 9.62SGYY45 pKa = 9.75PSYY48 pKa = 11.32FNDD51 pKa = 3.67GKK53 pKa = 11.23LSTRR57 pKa = 11.84WSSKK61 pKa = 9.39YY62 pKa = 10.58SSPQWIYY69 pKa = 11.33VDD71 pKa = 4.16LGSPRR76 pKa = 11.84TFDD79 pKa = 2.8QVVIHH84 pKa = 6.44WEE86 pKa = 3.43AAYY89 pKa = 10.49GEE91 pKa = 4.3NYY93 pKa = 9.55EE94 pKa = 4.23IQVSDD99 pKa = 4.62GASEE103 pKa = 4.15WTTVTAVNNGDD114 pKa = 3.73GGTDD118 pKa = 2.77ILTFPATEE126 pKa = 3.46ARR128 pKa = 11.84YY129 pKa = 9.76IRR131 pKa = 11.84MYY133 pKa = 11.1GEE135 pKa = 4.17ARR137 pKa = 11.84GTQYY141 pKa = 11.18GYY143 pKa = 10.37SIKK146 pKa = 10.42EE147 pKa = 3.78FEE149 pKa = 4.44VYY151 pKa = 10.67LINEE155 pKa = 4.7PPTADD160 pKa = 3.15ADD162 pKa = 3.87GPYY165 pKa = 10.14EE166 pKa = 4.06VEE168 pKa = 3.66EE169 pKa = 4.68GEE171 pKa = 4.36NIKK174 pKa = 10.84LDD176 pKa = 3.84GTGSSDD182 pKa = 3.69PDD184 pKa = 3.99GDD186 pKa = 3.99SLSYY190 pKa = 10.8SWTITNDD197 pKa = 3.34PTGEE201 pKa = 3.93ASLADD206 pKa = 4.02AEE208 pKa = 4.79TEE210 pKa = 4.14NPTLQAPSVGSGTDD224 pKa = 2.94IEE226 pKa = 4.6VQLTVDD232 pKa = 4.38DD233 pKa = 4.27GHH235 pKa = 7.04GNTDD239 pKa = 3.01TDD241 pKa = 3.93TATVTVQNVPEE252 pKa = 4.69EE253 pKa = 4.37GFPWTTVAVVVIVIIVILIVGIWLRR278 pKa = 11.84QSGQLASEE286 pKa = 4.69GSGKK290 pKa = 10.55SSS292 pKa = 2.98

Molecular weight: 31.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133U460|A0A133U460_9EURY DNA ligase (Fragment) OS=candidate division MSBL1 archaeon SCGC-AAA259A05 OX=1698259 GN=AKJ57_06015 PE=3 SV=1
MM1 pKa = 7.36ARR3 pKa = 11.84KK4 pKa = 9.78SSLKK8 pKa = 10.35KK9 pKa = 9.32IALEE13 pKa = 3.89RR14 pKa = 11.84VEE16 pKa = 4.85RR17 pKa = 11.84LLDD20 pKa = 3.66FASSIFKK27 pKa = 10.35RR28 pKa = 11.84RR29 pKa = 11.84PDD31 pKa = 3.28LAHH34 pKa = 7.48RR35 pKa = 11.84YY36 pKa = 9.84AEE38 pKa = 4.3LAWKK42 pKa = 10.23IKK44 pKa = 9.89IRR46 pKa = 11.84YY47 pKa = 7.54NLRR50 pKa = 11.84LPRR53 pKa = 11.84RR54 pKa = 11.84LKK56 pKa = 10.6RR57 pKa = 11.84KK58 pKa = 9.21FCRR61 pKa = 11.84KK62 pKa = 9.14CQSFWVPGEE71 pKa = 4.0TCRR74 pKa = 11.84VRR76 pKa = 11.84LRR78 pKa = 11.84PSSPPHH84 pKa = 6.23IAITCLKK91 pKa = 8.88CGHH94 pKa = 5.76VQRR97 pKa = 11.84IPYY100 pKa = 9.85EE101 pKa = 4.0EE102 pKa = 3.97

Molecular weight: 12.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1212

0

1212

270658

29

1259

223.3

25.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.333 ± 0.078

1.04 ± 0.03

5.593 ± 0.051

10.234 ± 0.103

3.891 ± 0.055

7.698 ± 0.066

1.708 ± 0.031

6.632 ± 0.067

7.211 ± 0.086

9.312 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.241 ± 0.034

3.568 ± 0.046

4.159 ± 0.049

2.16 ± 0.032

5.974 ± 0.074

6.558 ± 0.061

4.59 ± 0.046

6.999 ± 0.067

1.214 ± 0.032

2.886 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski