Amino acid dipepetide frequency for Pseudomonad phage gh-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.75AlaAla: 10.75 ± 1.289
0.762AlaCys: 0.762 ± 0.309
6.687AlaAsp: 6.687 ± 1.029
5.671AlaGlu: 5.671 ± 0.689
2.37AlaPhe: 2.37 ± 0.501
8.126AlaGly: 8.126 ± 0.825
1.185AlaHis: 1.185 ± 0.318
4.909AlaIle: 4.909 ± 0.715
5.587AlaLys: 5.587 ± 0.648
8.888AlaLeu: 8.888 ± 1.136
2.709AlaMet: 2.709 ± 0.566
4.571AlaAsn: 4.571 ± 0.663
3.555AlaPro: 3.555 ± 0.529
5.079AlaGln: 5.079 ± 0.622
5.587AlaArg: 5.587 ± 0.665
6.264AlaSer: 6.264 ± 1.495
6.941AlaThr: 6.941 ± 0.815
5.671AlaVal: 5.671 ± 0.686
1.354AlaTrp: 1.354 ± 0.344
2.539AlaTyr: 2.539 ± 0.396
0.0AlaXaa: 0.0 ± 0.0
Cys
1.27CysAla: 1.27 ± 0.338
0.0CysCys: 0.0 ± 0.0
0.508CysAsp: 0.508 ± 0.328
0.423CysGlu: 0.423 ± 0.179
0.508CysPhe: 0.508 ± 0.227
0.508CysGly: 0.508 ± 0.243
0.169CysHis: 0.169 ± 0.116
0.254CysIle: 0.254 ± 0.151
0.677CysLys: 0.677 ± 0.317
0.762CysLeu: 0.762 ± 0.297
0.169CysMet: 0.169 ± 0.129
0.423CysAsn: 0.423 ± 0.179
0.339CysPro: 0.339 ± 0.22
0.508CysGln: 0.508 ± 0.204
0.846CysArg: 0.846 ± 0.449
0.339CysSer: 0.339 ± 0.188
0.085CysThr: 0.085 ± 0.091
0.677CysVal: 0.677 ± 0.286
0.0CysTrp: 0.0 ± 0.0
0.254CysTyr: 0.254 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
5.756AspAla: 5.756 ± 0.661
0.593AspCys: 0.593 ± 0.338
4.486AspAsp: 4.486 ± 0.599
3.978AspGlu: 3.978 ± 0.89
3.047AspPhe: 3.047 ± 0.564
6.01AspGly: 6.01 ± 0.59
1.016AspHis: 1.016 ± 0.308
4.148AspIle: 4.148 ± 0.521
3.809AspLys: 3.809 ± 0.571
4.994AspLeu: 4.994 ± 0.685
1.947AspMet: 1.947 ± 0.353
2.455AspAsn: 2.455 ± 0.316
2.709AspPro: 2.709 ± 0.829
2.116AspGln: 2.116 ± 0.436
2.793AspArg: 2.793 ± 0.459
4.063AspSer: 4.063 ± 0.635
3.132AspThr: 3.132 ± 0.38
4.571AspVal: 4.571 ± 0.691
1.524AspTrp: 1.524 ± 0.334
1.524AspTyr: 1.524 ± 0.335
0.0AspXaa: 0.0 ± 0.0
Glu
8.126GluAla: 8.126 ± 0.886
0.593GluCys: 0.593 ± 0.222
4.402GluAsp: 4.402 ± 0.412
3.724GluGlu: 3.724 ± 0.908
2.963GluPhe: 2.963 ± 0.466
4.825GluGly: 4.825 ± 0.934
1.524GluHis: 1.524 ± 0.409
3.217GluIle: 3.217 ± 0.423
2.793GluLys: 2.793 ± 0.429
4.909GluLeu: 4.909 ± 0.894
2.201GluMet: 2.201 ± 0.413
2.455GluAsn: 2.455 ± 0.396
1.608GluPro: 1.608 ± 0.29
3.47GluGln: 3.47 ± 0.575
4.74GluArg: 4.74 ± 0.671
4.063GluSer: 4.063 ± 0.686
3.978GluThr: 3.978 ± 0.552
3.724GluVal: 3.724 ± 0.673
1.354GluTrp: 1.354 ± 0.376
2.709GluTyr: 2.709 ± 0.487
0.0GluXaa: 0.0 ± 0.0
Phe
2.878PheAla: 2.878 ± 0.518
0.508PheCys: 0.508 ± 0.214
2.539PheAsp: 2.539 ± 0.528
1.778PheGlu: 1.778 ± 0.434
1.185PhePhe: 1.185 ± 0.321
3.047PheGly: 3.047 ± 0.451
0.931PheHis: 0.931 ± 0.261
1.524PheIle: 1.524 ± 0.372
1.524PheLys: 1.524 ± 0.415
2.116PheLeu: 2.116 ± 0.441
1.185PheMet: 1.185 ± 0.347
1.608PheAsn: 1.608 ± 0.404
2.201PhePro: 2.201 ± 0.41
1.185PheGln: 1.185 ± 0.389
2.37PheArg: 2.37 ± 0.484
2.37PheSer: 2.37 ± 0.542
2.709PheThr: 2.709 ± 0.381
2.878PheVal: 2.878 ± 0.572
0.593PheTrp: 0.593 ± 0.232
0.846PheTyr: 0.846 ± 0.334
0.0PheXaa: 0.0 ± 0.0
Gly
7.11GlyAla: 7.11 ± 0.885
0.593GlyCys: 0.593 ± 0.246
5.502GlyAsp: 5.502 ± 0.508
5.417GlyGlu: 5.417 ± 0.548
3.301GlyPhe: 3.301 ± 0.62
6.348GlyGly: 6.348 ± 0.757
1.608GlyHis: 1.608 ± 0.46
4.825GlyIle: 4.825 ± 0.588
4.148GlyLys: 4.148 ± 0.56
6.264GlyLeu: 6.264 ± 0.76
1.947GlyMet: 1.947 ± 0.295
2.37GlyAsn: 2.37 ± 0.468
2.455GlyPro: 2.455 ± 0.49
2.624GlyGln: 2.624 ± 0.474
5.079GlyArg: 5.079 ± 0.641
6.518GlySer: 6.518 ± 0.942
4.74GlyThr: 4.74 ± 0.687
4.825GlyVal: 4.825 ± 0.557
1.862GlyTrp: 1.862 ± 0.333
3.132GlyTyr: 3.132 ± 0.568
0.0GlyXaa: 0.0 ± 0.0
His
1.524HisAla: 1.524 ± 0.339
0.423HisCys: 0.423 ± 0.189
1.27HisAsp: 1.27 ± 0.367
0.846HisGlu: 0.846 ± 0.238
0.762HisPhe: 0.762 ± 0.262
1.693HisGly: 1.693 ± 0.348
0.169HisHis: 0.169 ± 0.13
1.608HisIle: 1.608 ± 0.454
0.677HisLys: 0.677 ± 0.264
1.693HisLeu: 1.693 ± 0.474
1.185HisMet: 1.185 ± 0.28
0.846HisAsn: 0.846 ± 0.269
0.508HisPro: 0.508 ± 0.216
0.762HisGln: 0.762 ± 0.318
0.846HisArg: 0.846 ± 0.322
1.1HisSer: 1.1 ± 0.334
0.677HisThr: 0.677 ± 0.234
1.524HisVal: 1.524 ± 0.407
0.339HisTrp: 0.339 ± 0.162
0.677HisTyr: 0.677 ± 0.212
0.0HisXaa: 0.0 ± 0.0
Ile
5.333IleAla: 5.333 ± 0.479
0.762IleCys: 0.762 ± 0.255
3.555IleAsp: 3.555 ± 0.594
4.74IleGlu: 4.74 ± 0.757
1.1IlePhe: 1.1 ± 0.283
3.64IleGly: 3.64 ± 0.538
0.846IleHis: 0.846 ± 0.306
2.031IleIle: 2.031 ± 0.415
3.555IleLys: 3.555 ± 0.575
3.64IleLeu: 3.64 ± 0.604
0.593IleMet: 0.593 ± 0.197
2.201IleAsn: 2.201 ± 0.593
1.947IlePro: 1.947 ± 0.542
2.285IleGln: 2.285 ± 0.43
3.217IleArg: 3.217 ± 0.476
2.201IleSer: 2.201 ± 0.391
3.301IleThr: 3.301 ± 0.588
3.386IleVal: 3.386 ± 0.593
0.762IleTrp: 0.762 ± 0.276
1.693IleTyr: 1.693 ± 0.313
0.0IleXaa: 0.0 ± 0.0
Lys
5.841LysAla: 5.841 ± 0.827
0.339LysCys: 0.339 ± 0.158
3.894LysAsp: 3.894 ± 0.514
3.132LysGlu: 3.132 ± 0.593
2.455LysPhe: 2.455 ± 0.416
4.994LysGly: 4.994 ± 0.777
1.354LysHis: 1.354 ± 0.518
2.539LysIle: 2.539 ± 0.481
3.301LysLys: 3.301 ± 0.632
5.841LysLeu: 5.841 ± 0.646
1.1LysMet: 1.1 ± 0.264
1.947LysAsn: 1.947 ± 0.441
2.624LysPro: 2.624 ± 0.523
2.37LysGln: 2.37 ± 0.44
3.217LysArg: 3.217 ± 0.587
2.878LysSer: 2.878 ± 0.501
3.386LysThr: 3.386 ± 0.488
4.571LysVal: 4.571 ± 0.595
0.593LysTrp: 0.593 ± 0.264
1.439LysTyr: 1.439 ± 0.261
0.0LysXaa: 0.0 ± 0.0
Leu
7.449LeuAla: 7.449 ± 0.779
0.593LeuCys: 0.593 ± 0.262
4.148LeuAsp: 4.148 ± 0.518
5.417LeuGlu: 5.417 ± 0.836
2.031LeuPhe: 2.031 ± 0.529
5.248LeuGly: 5.248 ± 0.666
1.947LeuHis: 1.947 ± 0.353
5.248LeuIle: 5.248 ± 0.648
6.518LeuLys: 6.518 ± 0.695
4.655LeuLeu: 4.655 ± 0.568
2.539LeuMet: 2.539 ± 0.423
4.148LeuAsn: 4.148 ± 0.695
2.624LeuPro: 2.624 ± 0.478
4.402LeuGln: 4.402 ± 0.585
5.587LeuArg: 5.587 ± 0.656
5.163LeuSer: 5.163 ± 0.692
3.978LeuThr: 3.978 ± 0.585
4.909LeuVal: 4.909 ± 0.829
0.931LeuTrp: 0.931 ± 0.294
1.693LeuTyr: 1.693 ± 0.413
0.0LeuXaa: 0.0 ± 0.0
Met
2.963MetAla: 2.963 ± 0.5
0.169MetCys: 0.169 ± 0.12
2.116MetAsp: 2.116 ± 0.403
2.031MetGlu: 2.031 ± 0.508
0.593MetPhe: 0.593 ± 0.312
1.693MetGly: 1.693 ± 0.352
0.593MetHis: 0.593 ± 0.192
1.27MetIle: 1.27 ± 0.314
1.185MetLys: 1.185 ± 0.38
2.624MetLeu: 2.624 ± 0.47
0.169MetMet: 0.169 ± 0.107
1.27MetAsn: 1.27 ± 0.274
1.354MetPro: 1.354 ± 0.29
1.1MetGln: 1.1 ± 0.385
0.846MetArg: 0.846 ± 0.268
2.201MetSer: 2.201 ± 0.438
2.285MetThr: 2.285 ± 0.383
1.354MetVal: 1.354 ± 0.271
0.085MetTrp: 0.085 ± 0.06
0.339MetTyr: 0.339 ± 0.185
0.0MetXaa: 0.0 ± 0.0
Asn
3.978AsnAla: 3.978 ± 0.571
0.593AsnCys: 0.593 ± 0.203
1.778AsnAsp: 1.778 ± 0.45
3.301AsnGlu: 3.301 ± 0.722
1.439AsnPhe: 1.439 ± 0.301
4.74AsnGly: 4.74 ± 0.811
0.423AsnHis: 0.423 ± 0.19
1.862AsnIle: 1.862 ± 0.334
1.016AsnLys: 1.016 ± 0.274
2.878AsnLeu: 2.878 ± 0.462
1.016AsnMet: 1.016 ± 0.351
2.285AsnAsn: 2.285 ± 0.482
2.793AsnPro: 2.793 ± 0.459
1.947AsnGln: 1.947 ± 0.438
2.201AsnArg: 2.201 ± 0.494
2.963AsnSer: 2.963 ± 0.75
1.862AsnThr: 1.862 ± 0.495
2.37AsnVal: 2.37 ± 0.532
0.423AsnTrp: 0.423 ± 0.16
1.524AsnTyr: 1.524 ± 0.432
0.0AsnXaa: 0.0 ± 0.0
Pro
3.386ProAla: 3.386 ± 0.502
0.254ProCys: 0.254 ± 0.185
3.555ProAsp: 3.555 ± 0.576
4.486ProGlu: 4.486 ± 0.568
1.439ProPhe: 1.439 ± 0.283
2.201ProGly: 2.201 ± 0.371
1.27ProHis: 1.27 ± 0.414
1.524ProIle: 1.524 ± 0.364
2.37ProLys: 2.37 ± 0.475
2.624ProLeu: 2.624 ± 0.437
0.931ProMet: 0.931 ± 0.265
1.693ProAsn: 1.693 ± 0.42
0.762ProPro: 0.762 ± 0.22
1.608ProGln: 1.608 ± 0.331
1.862ProArg: 1.862 ± 0.423
1.947ProSer: 1.947 ± 0.347
1.439ProThr: 1.439 ± 0.375
2.709ProVal: 2.709 ± 0.453
0.508ProTrp: 0.508 ± 0.268
1.185ProTyr: 1.185 ± 0.387
0.0ProXaa: 0.0 ± 0.0
Gln
6.348GlnAla: 6.348 ± 0.807
0.423GlnCys: 0.423 ± 0.237
2.285GlnAsp: 2.285 ± 0.389
2.963GlnGlu: 2.963 ± 0.641
2.201GlnPhe: 2.201 ± 0.338
4.063GlnGly: 4.063 ± 0.57
0.423GlnHis: 0.423 ± 0.194
1.608GlnIle: 1.608 ± 0.383
2.285GlnLys: 2.285 ± 0.735
3.978GlnLeu: 3.978 ± 0.651
0.931GlnMet: 0.931 ± 0.253
1.439GlnAsn: 1.439 ± 0.336
1.185GlnPro: 1.185 ± 0.178
2.878GlnGln: 2.878 ± 0.701
2.624GlnArg: 2.624 ± 0.68
2.624GlnSer: 2.624 ± 0.659
1.862GlnThr: 1.862 ± 0.396
2.539GlnVal: 2.539 ± 0.362
0.677GlnTrp: 0.677 ± 0.281
2.031GlnTyr: 2.031 ± 0.388
0.0GlnXaa: 0.0 ± 0.0
Arg
5.756ArgAla: 5.756 ± 0.622
0.423ArgCys: 0.423 ± 0.205
3.47ArgAsp: 3.47 ± 0.395
4.74ArgGlu: 4.74 ± 0.8
2.37ArgPhe: 2.37 ± 0.507
4.825ArgGly: 4.825 ± 0.587
1.185ArgHis: 1.185 ± 0.365
2.455ArgIle: 2.455 ± 0.424
3.724ArgLys: 3.724 ± 0.623
4.655ArgLeu: 4.655 ± 0.539
1.693ArgMet: 1.693 ± 0.286
2.201ArgAsn: 2.201 ± 0.361
1.693ArgPro: 1.693 ± 0.384
3.217ArgGln: 3.217 ± 0.585
3.217ArgArg: 3.217 ± 0.354
4.655ArgSer: 4.655 ± 0.592
1.862ArgThr: 1.862 ± 0.497
3.978ArgVal: 3.978 ± 0.567
0.593ArgTrp: 0.593 ± 0.191
1.862ArgTyr: 1.862 ± 0.329
0.0ArgXaa: 0.0 ± 0.0
Ser
6.264SerAla: 6.264 ± 1.492
0.593SerCys: 0.593 ± 0.257
4.825SerAsp: 4.825 ± 0.537
3.555SerGlu: 3.555 ± 0.465
2.793SerPhe: 2.793 ± 0.503
6.941SerGly: 6.941 ± 1.087
1.862SerHis: 1.862 ± 0.32
3.047SerIle: 3.047 ± 0.431
3.132SerLys: 3.132 ± 0.523
4.909SerLeu: 4.909 ± 0.607
0.931SerMet: 0.931 ± 0.322
2.709SerAsn: 2.709 ± 0.423
2.116SerPro: 2.116 ± 0.317
2.624SerGln: 2.624 ± 0.619
2.878SerArg: 2.878 ± 0.466
4.063SerSer: 4.063 ± 0.831
2.285SerThr: 2.285 ± 0.455
3.978SerVal: 3.978 ± 0.597
0.931SerTrp: 0.931 ± 0.194
2.455SerTyr: 2.455 ± 0.583
0.0SerXaa: 0.0 ± 0.0
Thr
3.978ThrAla: 3.978 ± 0.663
0.254ThrCys: 0.254 ± 0.13
3.555ThrAsp: 3.555 ± 0.601
3.724ThrGlu: 3.724 ± 0.531
2.37ThrPhe: 2.37 ± 0.534
4.148ThrGly: 4.148 ± 0.592
0.677ThrHis: 0.677 ± 0.209
4.063ThrIle: 4.063 ± 0.686
4.909ThrLys: 4.909 ± 0.568
3.301ThrLeu: 3.301 ± 0.495
1.185ThrMet: 1.185 ± 0.308
2.539ThrAsn: 2.539 ± 0.613
2.539ThrPro: 2.539 ± 0.387
1.862ThrGln: 1.862 ± 0.431
2.539ThrArg: 2.539 ± 0.447
3.47ThrSer: 3.47 ± 0.517
2.709ThrThr: 2.709 ± 0.589
4.571ThrVal: 4.571 ± 0.472
0.508ThrTrp: 0.508 ± 0.192
1.27ThrTyr: 1.27 ± 0.248
0.0ThrXaa: 0.0 ± 0.0
Val
6.179ValAla: 6.179 ± 0.809
0.508ValCys: 0.508 ± 0.181
2.793ValAsp: 2.793 ± 0.428
4.74ValGlu: 4.74 ± 0.641
1.608ValPhe: 1.608 ± 0.384
4.402ValGly: 4.402 ± 0.559
0.846ValHis: 0.846 ± 0.323
2.963ValIle: 2.963 ± 0.663
3.724ValLys: 3.724 ± 0.482
5.587ValLeu: 5.587 ± 0.644
2.285ValMet: 2.285 ± 0.402
2.37ValAsn: 2.37 ± 0.531
3.386ValPro: 3.386 ± 0.719
2.963ValGln: 2.963 ± 0.406
5.587ValArg: 5.587 ± 0.555
3.809ValSer: 3.809 ± 0.509
4.063ValThr: 4.063 ± 0.608
4.232ValVal: 4.232 ± 0.804
1.016ValTrp: 1.016 ± 0.27
2.201ValTyr: 2.201 ± 0.497
0.0ValXaa: 0.0 ± 0.0
Trp
1.354TrpAla: 1.354 ± 0.324
0.0TrpCys: 0.0 ± 0.0
1.27TrpAsp: 1.27 ± 0.29
0.762TrpGlu: 0.762 ± 0.236
0.508TrpPhe: 0.508 ± 0.187
0.677TrpGly: 0.677 ± 0.23
0.508TrpHis: 0.508 ± 0.21
0.677TrpIle: 0.677 ± 0.213
0.846TrpLys: 0.846 ± 0.207
1.947TrpLeu: 1.947 ± 0.443
0.423TrpMet: 0.423 ± 0.159
0.508TrpAsn: 0.508 ± 0.15
0.339TrpPro: 0.339 ± 0.17
0.593TrpGln: 0.593 ± 0.225
1.1TrpArg: 1.1 ± 0.307
0.931TrpSer: 0.931 ± 0.304
0.846TrpThr: 0.846 ± 0.295
0.931TrpVal: 0.931 ± 0.243
0.254TrpTrp: 0.254 ± 0.137
0.254TrpTyr: 0.254 ± 0.174
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.047TyrAla: 3.047 ± 0.516
0.339TyrCys: 0.339 ± 0.142
2.031TyrAsp: 2.031 ± 0.446
1.947TyrGlu: 1.947 ± 0.424
0.931TyrPhe: 0.931 ± 0.274
2.539TyrGly: 2.539 ± 0.485
0.593TyrHis: 0.593 ± 0.224
1.016TyrIle: 1.016 ± 0.317
2.031TyrLys: 2.031 ± 0.363
2.963TyrLeu: 2.963 ± 0.514
1.016TyrMet: 1.016 ± 0.287
1.354TyrAsn: 1.354 ± 0.236
1.016TyrPro: 1.016 ± 0.251
1.778TyrGln: 1.778 ± 0.401
1.439TyrArg: 1.439 ± 0.249
1.27TyrSer: 1.27 ± 0.336
2.031TyrThr: 2.031 ± 0.475
1.862TyrVal: 1.862 ± 0.42
0.423TyrTrp: 0.423 ± 0.187
0.423TyrTyr: 0.423 ± 0.171
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 42 proteins (11815 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski