Bacillus clausii (strain KSM-K16)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus; Alkalihalobacillus clausii

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4082 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5WDP8|Q5WDP8_BACSK ABC transporter ATP-binding protein OS=Bacillus clausii (strain KSM-K16) OX=66692 GN=ABC2978 PE=3 SV=1
MM1 pKa = 7.62KK2 pKa = 10.55AFLKK6 pKa = 10.07TLGVTTAAIALVACGQGGNEE26 pKa = 3.75QGGANGDD33 pKa = 3.68GGNADD38 pKa = 4.04EE39 pKa = 4.8PTTIKK44 pKa = 10.38ISASNVPHH52 pKa = 7.87AEE54 pKa = 3.8ILEE57 pKa = 4.22EE58 pKa = 4.63AEE60 pKa = 4.47PLLKK64 pKa = 10.57EE65 pKa = 3.85QGVEE69 pKa = 3.97LEE71 pKa = 4.34IEE73 pKa = 4.02IAQDD77 pKa = 3.5YY78 pKa = 9.14VLPNIALNDD87 pKa = 3.64GDD89 pKa = 4.14VDD91 pKa = 3.93ANYY94 pKa = 9.59FQHH97 pKa = 6.97RR98 pKa = 11.84PYY100 pKa = 10.94LEE102 pKa = 4.2EE103 pKa = 4.01QLEE106 pKa = 4.09QNPDD110 pKa = 3.19YY111 pKa = 11.58DD112 pKa = 3.96FVEE115 pKa = 4.76AGAIHH120 pKa = 7.0LEE122 pKa = 3.88PMGLYY127 pKa = 9.24SQEE130 pKa = 3.92YY131 pKa = 10.48DD132 pKa = 4.36SVDD135 pKa = 3.95DD136 pKa = 4.91LPDD139 pKa = 3.83GATVMMSDD147 pKa = 3.44SVADD151 pKa = 3.56HH152 pKa = 6.0GRR154 pKa = 11.84VYY156 pKa = 11.2SLLQEE161 pKa = 4.73AGLITLKK168 pKa = 10.84EE169 pKa = 4.28GVSVDD174 pKa = 3.09ATEE177 pKa = 4.42QDD179 pKa = 3.51IVDD182 pKa = 3.76NPKK185 pKa = 10.49NLTFVNSGVAAEE197 pKa = 5.19LLPQAYY203 pKa = 8.02TNNEE207 pKa = 3.66ADD209 pKa = 4.92LIAINSNYY217 pKa = 10.45AIDD220 pKa = 4.8AGLSPTEE227 pKa = 4.07DD228 pKa = 3.0SVILEE233 pKa = 4.62EE234 pKa = 5.48GDD236 pKa = 3.83AEE238 pKa = 4.11NPYY241 pKa = 10.96VNLIVVRR248 pKa = 11.84SEE250 pKa = 4.24DD251 pKa = 3.73EE252 pKa = 4.0NNEE255 pKa = 4.2AIQTLVEE262 pKa = 4.14VLQSEE267 pKa = 4.63EE268 pKa = 3.54IKK270 pKa = 10.23TFIEE274 pKa = 4.13EE275 pKa = 4.29NYY277 pKa = 9.85GGAVIPAEE285 pKa = 4.05

Molecular weight: 30.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5WAJ5|Q5WAJ5_BACSK HTH merR-type domain-containing protein OS=Bacillus clausii (strain KSM-K16) OX=66692 GN=ABC4084 PE=4 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.34RR13 pKa = 11.84KK14 pKa = 8.16KK15 pKa = 9.1NHH17 pKa = 4.73GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37NGRR29 pKa = 11.84KK30 pKa = 8.82VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84QKK38 pKa = 10.39GRR40 pKa = 11.84KK41 pKa = 8.52VLSAA45 pKa = 4.05

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4082

0

4082

1225128

26

2870

300.1

33.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.796 ± 0.042

0.775 ± 0.011

4.977 ± 0.033

7.428 ± 0.046

4.453 ± 0.034

7.199 ± 0.038

2.263 ± 0.022

6.69 ± 0.032

5.764 ± 0.036

10.061 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.704 ± 0.018

3.727 ± 0.026

3.852 ± 0.021

4.012 ± 0.029

4.447 ± 0.028

5.667 ± 0.027

5.511 ± 0.031

7.249 ± 0.03

1.098 ± 0.015

3.327 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski