Amino acid dipepetide frequency for Bacillus clausii (strain KSM-K16)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.245AlaAla: 8.245 ± 0.106	0.899AlaCys: 0.899 ± 0.028	4.475AlaAsp: 4.475 ± 0.149	5.852AlaGlu: 5.852 ± 0.076	4.333AlaPhe: 4.333 ± 0.073	6.377AlaGly: 6.377 ± 0.073	1.757AlaHis: 1.757 ± 0.042	6.741AlaIle: 6.741 ± 0.068	5.312AlaLys: 5.312 ± 0.071	9.474AlaLeu: 9.474 ± 0.094	2.406AlaMet: 2.406 ± 0.045	3.43AlaAsn: 3.43 ± 0.054	2.65AlaPro: 2.65 ± 0.051	2.923AlaGln: 2.923 ± 0.046	3.412AlaArg: 3.412 ± 0.064	5.002AlaSer: 5.002 ± 0.061	4.482AlaThr: 4.482 ± 0.057	6.567AlaVal: 6.567 ± 0.084	0.753AlaTrp: 0.753 ± 0.025	2.868AlaTyr: 2.868 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.633CysAla: 0.633 ± 0.024	0.104CysCys: 0.104 ± 0.01	0.398CysAsp: 0.398 ± 0.017	0.458CysGlu: 0.458 ± 0.021	0.365CysPhe: 0.365 ± 0.017	0.748CysGly: 0.748 ± 0.027	0.217CysHis: 0.217 ± 0.013	0.482CysIle: 0.482 ± 0.021	0.354CysLys: 0.354 ± 0.017	0.797CysLeu: 0.797 ± 0.024	0.202CysMet: 0.202 ± 0.014	0.247CysAsn: 0.247 ± 0.015	0.368CysPro: 0.368 ± 0.02	0.292CysGln: 0.292 ± 0.015	0.354CysArg: 0.354 ± 0.016	0.488CysSer: 0.488 ± 0.022	0.424CysThr: 0.424 ± 0.019	0.486CysVal: 0.486 ± 0.022	0.078CysTrp: 0.078 ± 0.008	0.258CysTyr: 0.258 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.926AspAla: 3.926 ± 0.151	0.375AspCys: 0.375 ± 0.02	2.508AspAsp: 2.508 ± 0.053	4.259AspGlu: 4.259 ± 0.073	2.183AspPhe: 2.183 ± 0.044	3.72AspGly: 3.72 ± 0.064	1.215AspHis: 1.215 ± 0.03	3.458AspIle: 3.458 ± 0.053	2.624AspLys: 2.624 ± 0.053	4.617AspLeu: 4.617 ± 0.066	1.339AspMet: 1.339 ± 0.033	1.619AspAsn: 1.619 ± 0.036	2.083AspPro: 2.083 ± 0.046	1.852AspGln: 1.852 ± 0.043	2.365AspArg: 2.365 ± 0.034	2.43AspSer: 2.43 ± 0.049	2.395AspThr: 2.395 ± 0.062	4.114AspVal: 4.114 ± 0.058	0.677AspTrp: 0.677 ± 0.023	2.009AspTyr: 2.009 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	7.29GluAla: 7.29 ± 0.073	0.36GluCys: 0.36 ± 0.017	3.503GluAsp: 3.503 ± 0.066	6.998GluGlu: 6.998 ± 0.107	2.103GluPhe: 2.103 ± 0.047	4.548GluGly: 4.548 ± 0.077	1.628GluHis: 1.628 ± 0.037	4.222GluIle: 4.222 ± 0.073	5.184GluLys: 5.184 ± 0.069	7.206GluLeu: 7.206 ± 0.087	2.188GluMet: 2.188 ± 0.041	3.037GluAsn: 3.037 ± 0.054	2.423GluPro: 2.423 ± 0.062	4.262GluGln: 4.262 ± 0.065	4.266GluArg: 4.266 ± 0.062	3.354GluSer: 3.354 ± 0.06	4.315GluThr: 4.315 ± 0.066	4.591GluVal: 4.591 ± 0.063	0.955GluTrp: 0.955 ± 0.033	1.841GluTyr: 1.841 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	4.036PheAla: 4.036 ± 0.064	0.402PheCys: 0.402 ± 0.019	2.172PheAsp: 2.172 ± 0.041	2.563PheGlu: 2.563 ± 0.05	2.371PhePhe: 2.371 ± 0.057	3.271PheGly: 3.271 ± 0.064	1.078PheHis: 1.078 ± 0.032	3.249PheIle: 3.249 ± 0.062	1.94PheLys: 1.94 ± 0.044	4.621PheLeu: 4.621 ± 0.076	1.15PheMet: 1.15 ± 0.032	1.456PheAsn: 1.456 ± 0.034	1.727PhePro: 1.727 ± 0.037	1.695PheGln: 1.695 ± 0.04	1.593PheArg: 1.593 ± 0.036	3.134PheSer: 3.134 ± 0.058	2.437PheThr: 2.437 ± 0.043	3.439PheVal: 3.439 ± 0.055	0.495PheTrp: 0.495 ± 0.019	1.703PheTyr: 1.703 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	5.95GlyAla: 5.95 ± 0.084	0.686GlyCys: 0.686 ± 0.025	3.384GlyAsp: 3.384 ± 0.069	4.918GlyGlu: 4.918 ± 0.078	3.41GlyPhe: 3.41 ± 0.056	5.25GlyGly: 5.25 ± 0.082	1.585GlyHis: 1.585 ± 0.041	5.531GlyIle: 5.531 ± 0.083	4.51GlyLys: 4.51 ± 0.065	7.024GlyLeu: 7.024 ± 0.087	2.17GlyMet: 2.17 ± 0.045	2.676GlyAsn: 2.676 ± 0.055	2.093GlyPro: 2.093 ± 0.113	2.576GlyGln: 2.576 ± 0.051	2.967GlyArg: 2.967 ± 0.056	4.08GlySer: 4.08 ± 0.056	4.211GlyThr: 4.211 ± 0.069	5.358GlyVal: 5.358 ± 0.076	0.863GlyTrp: 0.863 ± 0.029	2.75GlyTyr: 2.75 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.765HisAla: 1.765 ± 0.037	0.229HisCys: 0.229 ± 0.015	1.055HisAsp: 1.055 ± 0.034	1.462HisGlu: 1.462 ± 0.035	1.152HisPhe: 1.152 ± 0.028	1.599HisGly: 1.599 ± 0.036	0.719HisHis: 0.719 ± 0.026	1.49HisIle: 1.49 ± 0.033	0.984HisLys: 0.984 ± 0.028	2.395HisLeu: 2.395 ± 0.044	0.589HisMet: 0.589 ± 0.025	0.654HisAsn: 0.654 ± 0.021	1.165HisPro: 1.165 ± 0.032	0.863HisGln: 0.863 ± 0.026	0.931HisArg: 0.931 ± 0.029	1.302HisSer: 1.302 ± 0.034	1.186HisThr: 1.186 ± 0.033	1.799HisVal: 1.799 ± 0.042	0.271HisTrp: 0.271 ± 0.018	1.025HisTyr: 1.025 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.647IleAla: 6.647 ± 0.088	0.663IleCys: 0.663 ± 0.027	4.048IleAsp: 4.048 ± 0.049	5.149IleGlu: 5.149 ± 0.068	2.383IlePhe: 2.383 ± 0.053	5.948IleGly: 5.948 ± 0.085	1.557IleHis: 1.557 ± 0.041	4.093IleIle: 4.093 ± 0.067	3.234IleLys: 3.234 ± 0.056	5.433IleLeu: 5.433 ± 0.088	1.511IleMet: 1.511 ± 0.033	2.398IleAsn: 2.398 ± 0.045	2.967IlePro: 2.967 ± 0.055	2.423IleGln: 2.423 ± 0.045	3.072IleArg: 3.072 ± 0.051	4.029IleSer: 4.029 ± 0.063	3.401IleThr: 3.401 ± 0.058	5.327IleVal: 5.327 ± 0.07	0.642IleTrp: 0.642 ± 0.029	1.978IleTyr: 1.978 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	5.273LysAla: 5.273 ± 0.063	0.254LysCys: 0.254 ± 0.012	2.747LysAsp: 2.747 ± 0.054	5.612LysGlu: 5.612 ± 0.083	1.156LysPhe: 1.156 ± 0.032	4.058LysGly: 4.058 ± 0.053	1.376LysHis: 1.376 ± 0.038	2.837LysIle: 2.837 ± 0.048	4.351LysLys: 4.351 ± 0.065	4.827LysLeu: 4.827 ± 0.06	1.605LysMet: 1.605 ± 0.035	2.241LysAsn: 2.241 ± 0.043	2.329LysPro: 2.329 ± 0.053	3.732LysGln: 3.732 ± 0.063	3.736LysArg: 3.736 ± 0.058	2.625LysSer: 2.625 ± 0.048	3.438LysThr: 3.438 ± 0.056	3.396LysVal: 3.396 ± 0.064	0.762LysTrp: 0.762 ± 0.025	1.285LysTyr: 1.285 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	9.735LeuAla: 9.735 ± 0.112	0.762LeuCys: 0.762 ± 0.028	4.769LeuAsp: 4.769 ± 0.052	6.7LeuGlu: 6.7 ± 0.097	5.303LeuPhe: 5.303 ± 0.084	6.588LeuGly: 6.588 ± 0.074	2.197LeuHis: 2.197 ± 0.044	6.332LeuIle: 6.332 ± 0.081	5.425LeuLys: 5.425 ± 0.07	11.394LeuLeu: 11.394 ± 0.145	2.485LeuMet: 2.485 ± 0.05	3.609LeuAsn: 3.609 ± 0.056	4.358LeuPro: 4.358 ± 0.068	3.873LeuGln: 3.873 ± 0.049	3.942LeuArg: 3.942 ± 0.054	6.486LeuSer: 6.486 ± 0.072	5.612LeuThr: 5.612 ± 0.078	6.949LeuVal: 6.949 ± 0.089	0.861LeuTrp: 0.861 ± 0.029	3.226LeuTyr: 3.226 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	2.55MetAla: 2.55 ± 0.05	0.143MetCys: 0.143 ± 0.012	1.373MetAsp: 1.373 ± 0.035	2.063MetGlu: 2.063 ± 0.046	1.061MetPhe: 1.061 ± 0.03	1.659MetGly: 1.659 ± 0.045	0.481MetHis: 0.481 ± 0.018	1.743MetIle: 1.743 ± 0.04	2.055MetLys: 2.055 ± 0.039	2.712MetLeu: 2.712 ± 0.053	0.783MetMet: 0.783 ± 0.027	1.362MetAsn: 1.362 ± 0.031	1.13MetPro: 1.13 ± 0.033	0.855MetGln: 0.855 ± 0.029	1.242MetArg: 1.242 ± 0.03	1.56MetSer: 1.56 ± 0.036	1.542MetThr: 1.542 ± 0.041	1.819MetVal: 1.819 ± 0.038	0.199MetTrp: 0.199 ± 0.012	0.71MetTyr: 0.71 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.827AsnAla: 2.827 ± 0.055	0.262AsnCys: 0.262 ± 0.015	2.05AsnAsp: 2.05 ± 0.041	3.379AsnGlu: 3.379 ± 0.05	1.082AsnPhe: 1.082 ± 0.03	3.321AsnGly: 3.321 ± 0.059	0.944AsnHis: 0.944 ± 0.028	2.403AsnIle: 2.403 ± 0.047	2.084AsnLys: 2.084 ± 0.044	2.826AsnLeu: 2.826 ± 0.052	1.027AsnMet: 1.027 ± 0.03	1.456AsnAsn: 1.456 ± 0.04	1.774AsnPro: 1.774 ± 0.04	1.644AsnGln: 1.644 ± 0.041	1.992AsnArg: 1.992 ± 0.039	1.696AsnSer: 1.696 ± 0.036	1.829AsnThr: 1.829 ± 0.043	2.987AsnVal: 2.987 ± 0.053	0.544AsnTrp: 0.544 ± 0.023	1.139AsnTyr: 1.139 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	2.904ProAla: 2.904 ± 0.046	0.215ProCys: 0.215 ± 0.013	2.101ProAsp: 2.101 ± 0.045	2.983ProGlu: 2.983 ± 0.064	2.259ProPhe: 2.259 ± 0.044	2.599ProGly: 2.599 ± 0.053	0.94ProHis: 0.94 ± 0.029	2.651ProIle: 2.651 ± 0.051	1.934ProLys: 1.934 ± 0.044	4.209ProLeu: 4.209 ± 0.062	0.837ProMet: 0.837 ± 0.029	1.542ProAsn: 1.542 ± 0.042	1.274ProPro: 1.274 ± 0.036	1.226ProGln: 1.226 ± 0.042	1.257ProArg: 1.257 ± 0.036	2.38ProSer: 2.38 ± 0.044	2.29ProThr: 2.29 ± 0.107	3.039ProVal: 3.039 ± 0.052	0.427ProTrp: 0.427 ± 0.017	1.458ProTyr: 1.458 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	4.067GlnAla: 4.067 ± 0.067	0.203GlnCys: 0.203 ± 0.013	1.549GlnAsp: 1.549 ± 0.039	3.018GlnGlu: 3.018 ± 0.055	1.632GlnPhe: 1.632 ± 0.037	2.432GlnGly: 2.432 ± 0.047	0.864GlnHis: 0.864 ± 0.024	2.35GlnIle: 2.35 ± 0.048	2.532GlnLys: 2.532 ± 0.048	4.679GlnLeu: 4.679 ± 0.08	1.194GlnMet: 1.194 ± 0.029	1.336GlnAsn: 1.336 ± 0.029	1.459GlnPro: 1.459 ± 0.043	2.163GlnGln: 2.163 ± 0.046	1.739GlnArg: 1.739 ± 0.036	2.212GlnSer: 2.212 ± 0.048	2.578GlnThr: 2.578 ± 0.048	2.512GlnVal: 2.512 ± 0.043	0.473GlnTrp: 0.473 ± 0.019	1.124GlnTyr: 1.124 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	3.226ArgAla: 3.226 ± 0.049	0.343ArgCys: 0.343 ± 0.017	2.066ArgAsp: 2.066 ± 0.044	3.143ArgGlu: 3.143 ± 0.05	2.284ArgPhe: 2.284 ± 0.045	2.585ArgGly: 2.585 ± 0.045	1.042ArgHis: 1.042 ± 0.036	3.08ArgIle: 3.08 ± 0.051	2.982ArgLys: 2.982 ± 0.048	4.943ArgLeu: 4.943 ± 0.057	1.425ArgMet: 1.425 ± 0.03	1.654ArgAsn: 1.654 ± 0.036	1.535ArgPro: 1.535 ± 0.036	1.957ArgGln: 1.957 ± 0.042	2.129ArgArg: 2.129 ± 0.042	2.444ArgSer: 2.444 ± 0.046	2.451ArgThr: 2.451 ± 0.049	2.927ArgVal: 2.927 ± 0.052	0.455ArgTrp: 0.455 ± 0.022	1.8ArgTyr: 1.8 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	4.338SerAla: 4.338 ± 0.068	0.427SerCys: 0.427 ± 0.02	2.702SerAsp: 2.702 ± 0.047	3.725SerGlu: 3.725 ± 0.054	3.18SerPhe: 3.18 ± 0.055	4.461SerGly: 4.461 ± 0.069	1.288SerHis: 1.288 ± 0.034	4.258SerIle: 4.258 ± 0.073	3.0SerLys: 3.0 ± 0.048	6.007SerLeu: 6.007 ± 0.07	1.659SerMet: 1.659 ± 0.037	1.985SerAsn: 1.985 ± 0.047	2.175SerPro: 2.175 ± 0.038	1.944SerGln: 1.944 ± 0.039	2.265SerArg: 2.265 ± 0.042	3.409SerSer: 3.409 ± 0.062	2.964SerThr: 2.964 ± 0.05	4.059SerVal: 4.059 ± 0.051	0.674SerTrp: 0.674 ± 0.022	2.148SerTyr: 2.148 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	4.755ThrAla: 4.755 ± 0.068	0.393ThrCys: 0.393 ± 0.017	2.731ThrAsp: 2.731 ± 0.059	3.629ThrGlu: 3.629 ± 0.056	2.723ThrPhe: 2.723 ± 0.053	4.631ThrGly: 4.631 ± 0.202	1.123ThrHis: 1.123 ± 0.032	4.358ThrIle: 4.358 ± 0.076	2.979ThrLys: 2.979 ± 0.052	5.503ThrLeu: 5.503 ± 0.068	1.395ThrMet: 1.395 ± 0.037	2.189ThrAsn: 2.189 ± 0.04	2.239ThrPro: 2.239 ± 0.042	1.454ThrGln: 1.454 ± 0.036	1.99ThrArg: 1.99 ± 0.037	3.016ThrSer: 3.016 ± 0.053	3.036ThrThr: 3.036 ± 0.057	4.44ThrVal: 4.44 ± 0.071	0.536ThrTrp: 0.536 ± 0.023	1.988ThrTyr: 1.988 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	6.5ValAla: 6.5 ± 0.079	0.7ValCys: 0.7 ± 0.026	3.872ValAsp: 3.872 ± 0.067	4.975ValGlu: 4.975 ± 0.072	3.428ValPhe: 3.428 ± 0.057	4.913ValGly: 4.913 ± 0.074	1.535ValHis: 1.535 ± 0.033	4.951ValIle: 4.951 ± 0.066	3.756ValLys: 3.756 ± 0.07	7.448ValLeu: 7.448 ± 0.086	1.859ValMet: 1.859 ± 0.043	2.678ValAsn: 2.678 ± 0.049	3.033ValPro: 3.033 ± 0.051	2.476ValGln: 2.476 ± 0.046	2.988ValArg: 2.988 ± 0.051	4.53ValSer: 4.53 ± 0.06	4.119ValThr: 4.119 ± 0.071	5.611ValVal: 5.611 ± 0.081	0.764ValTrp: 0.764 ± 0.029	2.353ValTyr: 2.353 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.775TrpAla: 0.775 ± 0.027	0.069TrpCys: 0.069 ± 0.008	0.492TrpAsp: 0.492 ± 0.022	0.702TrpGlu: 0.702 ± 0.026	0.531TrpPhe: 0.531 ± 0.021	0.688TrpGly: 0.688 ± 0.022	0.298TrpHis: 0.298 ± 0.016	0.696TrpIle: 0.696 ± 0.026	0.657TrpLys: 0.657 ± 0.023	1.417TrpLeu: 1.417 ± 0.038	0.357TrpMet: 0.357 ± 0.018	0.499TrpAsn: 0.499 ± 0.021	0.364TrpPro: 0.364 ± 0.017	0.537TrpGln: 0.537 ± 0.019	0.526TrpArg: 0.526 ± 0.024	0.589TrpSer: 0.589 ± 0.021	0.588TrpThr: 0.588 ± 0.023	0.71TrpVal: 0.71 ± 0.024	0.149TrpTrp: 0.149 ± 0.011	0.34TrpTyr: 0.34 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.516TyrAla: 2.516 ± 0.051	0.264TyrCys: 0.264 ± 0.014	1.774TyrAsp: 1.774 ± 0.037	2.69TyrGlu: 2.69 ± 0.052	1.595TyrPhe: 1.595 ± 0.033	2.597TyrGly: 2.597 ± 0.046	0.788TyrHis: 0.788 ± 0.024	1.974TyrIle: 1.974 ± 0.037	1.739TyrLys: 1.739 ± 0.042	3.075TyrLeu: 3.075 ± 0.057	0.862TyrMet: 0.862 ± 0.024	1.198TyrAsn: 1.198 ± 0.032	1.379TyrPro: 1.379 ± 0.037	1.368TyrGln: 1.368 ± 0.033	1.703TyrArg: 1.703 ± 0.04	1.904TyrSer: 1.904 ± 0.044	1.812TyrThr: 1.812 ± 0.04	2.36TyrVal: 2.36 ± 0.047	0.405TyrTrp: 0.405 ± 0.017	1.261TyrTyr: 1.261 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4082 proteins (1225128 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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