Shewanella woodyi (strain ATCC 51908 / MS32)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella; Shewanella woodyi

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4865 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|B1KG88|GCSH_SHEWM Glycine cleavage system H protein OS=Shewanella woodyi (strain ATCC 51908 / MS32) OX=392500 GN=gcvH PE=3 SV=1
MM1 pKa = 7.28INKK4 pKa = 7.38VTFPLNCIALILASQLTGCGSDD26 pKa = 3.62GDD28 pKa = 4.52NDD30 pKa = 3.9SSTPTDD36 pKa = 3.38SAPPVITLVGNEE48 pKa = 4.31SIEE51 pKa = 4.11VVYY54 pKa = 10.96NAVFGDD60 pKa = 4.4LGAEE64 pKa = 4.01AHH66 pKa = 7.35DD67 pKa = 4.67DD68 pKa = 3.64TDD70 pKa = 4.05GSITVTTQGTVDD82 pKa = 3.05TSMPGTYY89 pKa = 9.85EE90 pKa = 3.64LTYY93 pKa = 10.4IATDD97 pKa = 3.27ASGNSSQATRR107 pKa = 11.84TVVVLEE113 pKa = 4.63DD114 pKa = 3.71TSQSATITTPGAPFSFFYY132 pKa = 11.12DD133 pKa = 3.8GVLKK137 pKa = 10.83GADD140 pKa = 3.48YY141 pKa = 10.12WSEE144 pKa = 3.93EE145 pKa = 3.95PQILSAGMGFDD156 pKa = 4.49GIVAIDD162 pKa = 3.93APEE165 pKa = 3.87LTIDD169 pKa = 3.67SVLAAGGAWSSDD181 pKa = 2.79IDD183 pKa = 4.52CGTGAQNYY191 pKa = 7.02TATSTRR197 pKa = 11.84DD198 pKa = 3.56QVSGAYY204 pKa = 9.5IIQTPYY210 pKa = 11.55GEE212 pKa = 4.97LKK214 pKa = 10.72DD215 pKa = 4.61DD216 pKa = 5.52AIGLDD221 pKa = 3.61GLPIVLSWPIDD232 pKa = 3.45TRR234 pKa = 11.84TLSLTDD240 pKa = 3.72FQFTLNTGDD249 pKa = 3.13IVRR252 pKa = 11.84PLAIGPLPNFEE263 pKa = 4.76DD264 pKa = 3.88NEE266 pKa = 4.51RR267 pKa = 11.84NTPVAFGEE275 pKa = 4.24FGNRR279 pKa = 11.84LPSDD283 pKa = 3.65HH284 pKa = 7.63PDD286 pKa = 2.61ARR288 pKa = 11.84FPIKK292 pKa = 10.78LEE294 pKa = 3.79IVEE297 pKa = 5.24DD298 pKa = 4.07DD299 pKa = 4.1TPLMMVGPGGQVVSAVGLTWEE320 pKa = 4.79TDD322 pKa = 3.06SSPYY326 pKa = 10.52DD327 pKa = 3.71EE328 pKa = 5.54NNGPRR333 pKa = 11.84LVGAKK338 pKa = 9.94LNRR341 pKa = 11.84IEE343 pKa = 4.09GQMDD347 pKa = 3.92GEE349 pKa = 5.14GISTLQPLIPANDD362 pKa = 3.31ATVLYY367 pKa = 10.79DD368 pKa = 3.94EE369 pKa = 5.2GDD371 pKa = 3.17FMLRR375 pKa = 11.84MLTSGGFSPDD385 pKa = 3.03GVSGLKK391 pKa = 10.23PNEE394 pKa = 3.6FEE396 pKa = 4.05RR397 pKa = 11.84FFRR400 pKa = 11.84IHH402 pKa = 7.35ANGLDD407 pKa = 3.78GNTVIIDD414 pKa = 3.77KK415 pKa = 10.93VGEE418 pKa = 4.07EE419 pKa = 4.44YY420 pKa = 10.77AVQGGTLRR428 pKa = 11.84VVGLSDD434 pKa = 4.53LGQPEE439 pKa = 4.1IGEE442 pKa = 4.43VTFDD446 pKa = 2.96ACYY449 pKa = 10.82SEE451 pKa = 6.1DD452 pKa = 3.62RR453 pKa = 11.84DD454 pKa = 3.81NYY456 pKa = 10.33IDD458 pKa = 4.51IILVGDD464 pKa = 3.79EE465 pKa = 3.66EE466 pKa = 4.25AARR469 pKa = 11.84NITILEE475 pKa = 4.16IPSLEE480 pKa = 4.03EE481 pKa = 4.56GYY483 pKa = 10.55SAFYY487 pKa = 10.93NPGGPGSTPFEE498 pKa = 4.24GVSYY502 pKa = 10.15SAPGPRR508 pKa = 11.84DD509 pKa = 3.65LEE511 pKa = 4.16PVIIALDD518 pKa = 3.5DD519 pKa = 3.99PMRR522 pKa = 11.84VTYY525 pKa = 10.7VPGSEE530 pKa = 4.13NVEE533 pKa = 4.08APDD536 pKa = 4.23NLMTFQFEE544 pKa = 4.62GIEE547 pKa = 4.21RR548 pKa = 11.84EE549 pKa = 4.02YY550 pKa = 11.21LLNVSSNYY558 pKa = 9.66TGDD561 pKa = 3.49VPVPLLFDD569 pKa = 3.43FHH571 pKa = 7.57GLNSAAEE578 pKa = 4.16VQYY581 pKa = 11.35SDD583 pKa = 3.91SQFNQISEE591 pKa = 4.44TEE593 pKa = 4.02NFILVTPQAINGWNVTGFPLGGNANDD619 pKa = 4.84LGFIDD624 pKa = 5.59ALIAQLSTAYY634 pKa = 10.82NIDD637 pKa = 3.36TNRR640 pKa = 11.84IYY642 pKa = 10.99AAGFSLGGFFSFEE655 pKa = 4.08LACQYY660 pKa = 11.38SDD662 pKa = 3.16TFAAIAPVSGVMTPAMAADD681 pKa = 4.57CVPEE685 pKa = 4.19RR686 pKa = 11.84PIPILQTHH694 pKa = 5.99GTADD698 pKa = 3.56DD699 pKa = 3.78QLPYY703 pKa = 10.92AQAQTVLQWWINFNQTDD720 pKa = 4.86LEE722 pKa = 4.2PVITDD727 pKa = 3.73LEE729 pKa = 4.4DD730 pKa = 3.61RR731 pKa = 11.84FPEE734 pKa = 4.32NGTTVQRR741 pKa = 11.84YY742 pKa = 8.43VYY744 pKa = 10.68GNGDD748 pKa = 3.27NGVSVEE754 pKa = 3.96HH755 pKa = 6.33LRR757 pKa = 11.84IEE759 pKa = 4.74GGQHH763 pKa = 5.6IWPGSAGDD771 pKa = 3.7SDD773 pKa = 4.18TNIAEE778 pKa = 4.63EE779 pKa = 3.59IWSFFEE785 pKa = 5.68SYY787 pKa = 11.11DD788 pKa = 4.03LNGKK792 pKa = 9.48INDD795 pKa = 3.64

Molecular weight: 85.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1KQ77|B1KQ77_SHEWM Uncharacterized protein OS=Shewanella woodyi (strain ATCC 51908 / MS32) OX=392500 GN=Swoo_0434 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPSTLKK12 pKa = 10.45RR13 pKa = 11.84KK14 pKa = 9.34RR15 pKa = 11.84SHH17 pKa = 6.2GFRR20 pKa = 11.84ARR22 pKa = 11.84MATVSGRR29 pKa = 11.84KK30 pKa = 8.84VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.39GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4865

0

4865

1675288

32

16322

344.4

38.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.369 ± 0.042

1.026 ± 0.016

5.67 ± 0.069

6.271 ± 0.042

4.135 ± 0.032

6.973 ± 0.059

2.151 ± 0.027

6.422 ± 0.032

5.29 ± 0.059

10.582 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.542 ± 0.024

4.365 ± 0.037

3.812 ± 0.022

4.43 ± 0.036

4.162 ± 0.048

7.262 ± 0.042

5.547 ± 0.106

6.683 ± 0.046

1.217 ± 0.017

3.091 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski