Amino acid dipepetide frequency for Gordonia phage Anon

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.89AlaAla: 9.89 ± 1.166
0.737AlaCys: 0.737 ± 0.258
6.389AlaAsp: 6.389 ± 0.7
7.556AlaGlu: 7.556 ± 0.863
3.01AlaPhe: 3.01 ± 0.583
8.293AlaGly: 8.293 ± 0.832
1.597AlaHis: 1.597 ± 0.272
4.976AlaIle: 4.976 ± 0.543
4.546AlaLys: 4.546 ± 0.739
8.539AlaLeu: 8.539 ± 0.991
2.641AlaMet: 2.641 ± 0.382
2.211AlaAsn: 2.211 ± 0.325
4.73AlaPro: 4.73 ± 0.807
3.624AlaGln: 3.624 ± 0.539
5.467AlaArg: 5.467 ± 0.549
4.669AlaSer: 4.669 ± 0.557
4.853AlaThr: 4.853 ± 0.587
6.204AlaVal: 6.204 ± 0.598
1.904AlaTrp: 1.904 ± 0.33
2.58AlaTyr: 2.58 ± 0.493
0.0AlaXaa: 0.0 ± 0.0
Cys
0.676CysAla: 0.676 ± 0.193
0.246CysCys: 0.246 ± 0.157
0.676CysAsp: 0.676 ± 0.277
0.369CysGlu: 0.369 ± 0.149
0.307CysPhe: 0.307 ± 0.17
0.983CysGly: 0.983 ± 0.262
0.246CysHis: 0.246 ± 0.125
0.246CysIle: 0.246 ± 0.111
0.307CysLys: 0.307 ± 0.13
0.614CysLeu: 0.614 ± 0.209
0.369CysMet: 0.369 ± 0.182
0.614CysAsn: 0.614 ± 0.183
0.676CysPro: 0.676 ± 0.264
0.184CysGln: 0.184 ± 0.113
0.491CysArg: 0.491 ± 0.189
0.307CysSer: 0.307 ± 0.132
0.491CysThr: 0.491 ± 0.191
0.553CysVal: 0.553 ± 0.147
0.246CysTrp: 0.246 ± 0.11
0.307CysTyr: 0.307 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
4.914AspAla: 4.914 ± 0.548
0.676AspCys: 0.676 ± 0.271
4.546AspAsp: 4.546 ± 0.567
4.914AspGlu: 4.914 ± 0.663
2.089AspPhe: 2.089 ± 0.316
5.651AspGly: 5.651 ± 0.469
1.781AspHis: 1.781 ± 0.396
3.071AspIle: 3.071 ± 0.475
2.457AspLys: 2.457 ± 0.369
5.467AspLeu: 5.467 ± 0.614
1.229AspMet: 1.229 ± 0.247
2.58AspAsn: 2.58 ± 0.445
4.73AspPro: 4.73 ± 0.591
2.211AspGln: 2.211 ± 0.34
3.256AspArg: 3.256 ± 0.404
3.44AspSer: 3.44 ± 0.562
4.791AspThr: 4.791 ± 0.559
4.607AspVal: 4.607 ± 0.444
1.229AspTrp: 1.229 ± 0.317
3.01AspTyr: 3.01 ± 0.424
0.0AspXaa: 0.0 ± 0.0
Glu
8.969GluAla: 8.969 ± 0.776
0.123GluCys: 0.123 ± 0.087
4.73GluAsp: 4.73 ± 0.689
4.116GluGlu: 4.116 ± 0.504
2.641GluPhe: 2.641 ± 0.369
5.099GluGly: 5.099 ± 0.511
1.843GluHis: 1.843 ± 0.447
3.809GluIle: 3.809 ± 0.488
2.826GluLys: 2.826 ± 0.396
7.064GluLeu: 7.064 ± 0.583
2.949GluMet: 2.949 ± 0.502
1.781GluAsn: 1.781 ± 0.341
2.519GluPro: 2.519 ± 0.494
2.211GluGln: 2.211 ± 0.423
3.931GluArg: 3.931 ± 0.562
2.826GluSer: 2.826 ± 0.393
5.099GluThr: 5.099 ± 0.512
5.713GluVal: 5.713 ± 0.528
0.86GluTrp: 0.86 ± 0.201
1.474GluTyr: 1.474 ± 0.312
0.0GluXaa: 0.0 ± 0.0
Phe
3.194PheAla: 3.194 ± 0.443
0.184PheCys: 0.184 ± 0.098
2.641PheAsp: 2.641 ± 0.448
3.133PheGlu: 3.133 ± 0.358
0.614PhePhe: 0.614 ± 0.188
2.519PheGly: 2.519 ± 0.356
1.044PheHis: 1.044 ± 0.258
1.351PheIle: 1.351 ± 0.315
1.536PheLys: 1.536 ± 0.332
2.089PheLeu: 2.089 ± 0.409
0.614PheMet: 0.614 ± 0.171
2.027PheAsn: 2.027 ± 0.293
2.027PhePro: 2.027 ± 0.287
0.799PheGln: 0.799 ± 0.206
1.72PheArg: 1.72 ± 0.297
2.089PheSer: 2.089 ± 0.369
2.027PheThr: 2.027 ± 0.386
2.027PheVal: 2.027 ± 0.33
0.553PheTrp: 0.553 ± 0.171
1.044PheTyr: 1.044 ± 0.286
0.0PheXaa: 0.0 ± 0.0
Gly
5.836GlyAla: 5.836 ± 0.691
0.737GlyCys: 0.737 ± 0.221
5.959GlyAsp: 5.959 ± 0.636
4.791GlyGlu: 4.791 ± 0.562
3.563GlyPhe: 3.563 ± 0.496
8.416GlyGly: 8.416 ± 1.394
2.089GlyHis: 2.089 ± 0.326
3.624GlyIle: 3.624 ± 0.524
3.809GlyLys: 3.809 ± 0.575
5.836GlyLeu: 5.836 ± 0.579
2.273GlyMet: 2.273 ± 0.52
2.396GlyAsn: 2.396 ± 0.341
3.379GlyPro: 3.379 ± 0.433
2.641GlyGln: 2.641 ± 0.352
4.116GlyArg: 4.116 ± 0.542
4.853GlySer: 4.853 ± 0.71
5.344GlyThr: 5.344 ± 0.693
5.651GlyVal: 5.651 ± 0.619
2.334GlyTrp: 2.334 ± 0.373
3.071GlyTyr: 3.071 ± 0.397
0.0GlyXaa: 0.0 ± 0.0
His
1.843HisAla: 1.843 ± 0.317
0.246HisCys: 0.246 ± 0.115
1.229HisAsp: 1.229 ± 0.271
1.781HisGlu: 1.781 ± 0.359
0.676HisPhe: 0.676 ± 0.197
1.781HisGly: 1.781 ± 0.381
0.553HisHis: 0.553 ± 0.184
1.044HisIle: 1.044 ± 0.218
0.737HisLys: 0.737 ± 0.219
1.536HisLeu: 1.536 ± 0.319
0.614HisMet: 0.614 ± 0.231
0.491HisAsn: 0.491 ± 0.206
1.474HisPro: 1.474 ± 0.295
0.737HisGln: 0.737 ± 0.235
1.659HisArg: 1.659 ± 0.295
1.106HisSer: 1.106 ± 0.221
1.29HisThr: 1.29 ± 0.261
1.536HisVal: 1.536 ± 0.266
0.184HisTrp: 0.184 ± 0.129
0.799HisTyr: 0.799 ± 0.269
0.0HisXaa: 0.0 ± 0.0
Ile
5.16IleAla: 5.16 ± 0.425
0.307IleCys: 0.307 ± 0.134
3.44IleAsp: 3.44 ± 0.48
4.239IleGlu: 4.239 ± 0.55
1.474IlePhe: 1.474 ± 0.245
4.546IleGly: 4.546 ± 0.601
1.106IleHis: 1.106 ± 0.263
1.966IleIle: 1.966 ± 0.377
2.396IleLys: 2.396 ± 0.329
3.563IleLeu: 3.563 ± 0.466
0.676IleMet: 0.676 ± 0.182
1.843IleAsn: 1.843 ± 0.351
3.256IlePro: 3.256 ± 0.395
1.72IleGln: 1.72 ± 0.303
3.563IleArg: 3.563 ± 0.539
3.317IleSer: 3.317 ± 0.353
3.256IleThr: 3.256 ± 0.431
3.501IleVal: 3.501 ± 0.47
0.86IleTrp: 0.86 ± 0.186
0.86IleTyr: 0.86 ± 0.193
0.0IleXaa: 0.0 ± 0.0
Lys
4.669LysAla: 4.669 ± 0.646
0.43LysCys: 0.43 ± 0.154
2.396LysAsp: 2.396 ± 0.331
2.641LysGlu: 2.641 ± 0.423
1.106LysPhe: 1.106 ± 0.273
3.563LysGly: 3.563 ± 0.405
1.044LysHis: 1.044 ± 0.227
2.273LysIle: 2.273 ± 0.391
2.58LysLys: 2.58 ± 0.5
4.669LysLeu: 4.669 ± 0.561
0.983LysMet: 0.983 ± 0.239
0.921LysAsn: 0.921 ± 0.266
3.194LysPro: 3.194 ± 0.458
2.15LysGln: 2.15 ± 0.39
2.58LysArg: 2.58 ± 0.436
2.334LysSer: 2.334 ± 0.361
2.764LysThr: 2.764 ± 0.39
3.501LysVal: 3.501 ± 0.504
0.676LysTrp: 0.676 ± 0.212
1.474LysTyr: 1.474 ± 0.316
0.0LysXaa: 0.0 ± 0.0
Leu
8.354LeuAla: 8.354 ± 0.715
0.799LeuCys: 0.799 ± 0.207
5.037LeuAsp: 5.037 ± 0.528
7.003LeuGlu: 7.003 ± 0.601
2.58LeuPhe: 2.58 ± 0.44
6.511LeuGly: 6.511 ± 0.573
1.536LeuHis: 1.536 ± 0.368
4.177LeuIle: 4.177 ± 0.549
3.194LeuLys: 3.194 ± 0.477
5.651LeuLeu: 5.651 ± 0.72
2.703LeuMet: 2.703 ± 0.487
2.396LeuAsn: 2.396 ± 0.396
4.423LeuPro: 4.423 ± 0.511
2.027LeuGln: 2.027 ± 0.329
5.529LeuArg: 5.529 ± 0.515
4.914LeuSer: 4.914 ± 0.448
4.73LeuThr: 4.73 ± 0.529
4.484LeuVal: 4.484 ± 0.534
1.781LeuTrp: 1.781 ± 0.342
2.089LeuTyr: 2.089 ± 0.379
0.0LeuXaa: 0.0 ± 0.0
Met
2.887MetAla: 2.887 ± 0.506
0.246MetCys: 0.246 ± 0.148
1.044MetAsp: 1.044 ± 0.223
0.737MetGlu: 0.737 ± 0.201
0.86MetPhe: 0.86 ± 0.255
1.106MetGly: 1.106 ± 0.288
0.614MetHis: 0.614 ± 0.176
1.72MetIle: 1.72 ± 0.344
1.72MetLys: 1.72 ± 0.367
1.659MetLeu: 1.659 ± 0.322
0.307MetMet: 0.307 ± 0.141
0.921MetAsn: 0.921 ± 0.246
1.966MetPro: 1.966 ± 0.368
0.983MetGln: 0.983 ± 0.239
1.044MetArg: 1.044 ± 0.206
2.334MetSer: 2.334 ± 0.318
2.519MetThr: 2.519 ± 0.395
1.106MetVal: 1.106 ± 0.2
0.184MetTrp: 0.184 ± 0.087
0.921MetTyr: 0.921 ± 0.215
0.0MetXaa: 0.0 ± 0.0
Asn
2.519AsnAla: 2.519 ± 0.375
0.491AsnCys: 0.491 ± 0.211
1.966AsnAsp: 1.966 ± 0.39
2.334AsnGlu: 2.334 ± 0.301
0.921AsnPhe: 0.921 ± 0.183
2.764AsnGly: 2.764 ± 0.453
0.43AsnHis: 0.43 ± 0.154
1.167AsnIle: 1.167 ± 0.264
1.474AsnLys: 1.474 ± 0.283
3.256AsnLeu: 3.256 ± 0.412
0.614AsnMet: 0.614 ± 0.225
1.044AsnAsn: 1.044 ± 0.254
2.519AsnPro: 2.519 ± 0.285
1.843AsnGln: 1.843 ± 0.331
2.273AsnArg: 2.273 ± 0.375
1.474AsnSer: 1.474 ± 0.313
2.273AsnThr: 2.273 ± 0.385
1.781AsnVal: 1.781 ± 0.316
0.43AsnTrp: 0.43 ± 0.158
1.474AsnTyr: 1.474 ± 0.304
0.0AsnXaa: 0.0 ± 0.0
Pro
5.283ProAla: 5.283 ± 0.558
0.369ProCys: 0.369 ± 0.155
3.809ProAsp: 3.809 ± 0.391
4.3ProGlu: 4.3 ± 0.502
1.659ProPhe: 1.659 ± 0.287
5.099ProGly: 5.099 ± 0.586
0.86ProHis: 0.86 ± 0.207
2.887ProIle: 2.887 ± 0.384
2.396ProLys: 2.396 ± 0.391
2.764ProLeu: 2.764 ± 0.447
1.536ProMet: 1.536 ± 0.284
2.396ProAsn: 2.396 ± 0.391
2.334ProPro: 2.334 ± 0.42
1.536ProGln: 1.536 ± 0.3
2.396ProArg: 2.396 ± 0.428
3.01ProSer: 3.01 ± 0.446
4.054ProThr: 4.054 ± 0.43
4.361ProVal: 4.361 ± 0.516
0.676ProTrp: 0.676 ± 0.196
1.72ProTyr: 1.72 ± 0.375
0.0ProXaa: 0.0 ± 0.0
Gln
3.747GlnAla: 3.747 ± 0.471
0.184GlnCys: 0.184 ± 0.096
1.659GlnAsp: 1.659 ± 0.345
2.15GlnGlu: 2.15 ± 0.398
1.229GlnPhe: 1.229 ± 0.304
2.089GlnGly: 2.089 ± 0.345
0.86GlnHis: 0.86 ± 0.198
2.519GlnIle: 2.519 ± 0.38
1.413GlnLys: 1.413 ± 0.282
3.379GlnLeu: 3.379 ± 0.482
0.676GlnMet: 0.676 ± 0.21
1.044GlnAsn: 1.044 ± 0.237
1.044GlnPro: 1.044 ± 0.272
1.351GlnGln: 1.351 ± 0.446
2.027GlnArg: 2.027 ± 0.332
1.904GlnSer: 1.904 ± 0.441
2.15GlnThr: 2.15 ± 0.403
2.764GlnVal: 2.764 ± 0.457
0.737GlnTrp: 0.737 ± 0.245
1.29GlnTyr: 1.29 ± 0.248
0.0GlnXaa: 0.0 ± 0.0
Arg
4.914ArgAla: 4.914 ± 0.535
0.676ArgCys: 0.676 ± 0.261
3.747ArgAsp: 3.747 ± 0.52
4.73ArgGlu: 4.73 ± 0.541
2.334ArgPhe: 2.334 ± 0.447
4.3ArgGly: 4.3 ± 0.581
2.15ArgHis: 2.15 ± 0.427
3.133ArgIle: 3.133 ± 0.381
2.826ArgLys: 2.826 ± 0.555
5.099ArgLeu: 5.099 ± 0.591
1.781ArgMet: 1.781 ± 0.34
2.15ArgAsn: 2.15 ± 0.415
2.457ArgPro: 2.457 ± 0.42
1.597ArgGln: 1.597 ± 0.325
4.791ArgArg: 4.791 ± 0.616
3.194ArgSer: 3.194 ± 0.453
2.826ArgThr: 2.826 ± 0.415
4.607ArgVal: 4.607 ± 0.418
1.044ArgTrp: 1.044 ± 0.278
2.334ArgTyr: 2.334 ± 0.472
0.0ArgXaa: 0.0 ± 0.0
Ser
5.651SerAla: 5.651 ± 0.582
0.491SerCys: 0.491 ± 0.224
3.563SerAsp: 3.563 ± 0.481
3.256SerGlu: 3.256 ± 0.428
2.273SerPhe: 2.273 ± 0.455
5.099SerGly: 5.099 ± 0.735
0.799SerHis: 0.799 ± 0.231
3.501SerIle: 3.501 ± 0.412
2.58SerLys: 2.58 ± 0.391
4.239SerLeu: 4.239 ± 0.619
1.106SerMet: 1.106 ± 0.25
1.966SerAsn: 1.966 ± 0.364
2.396SerPro: 2.396 ± 0.373
1.781SerGln: 1.781 ± 0.328
4.3SerArg: 4.3 ± 0.49
3.563SerSer: 3.563 ± 0.486
3.256SerThr: 3.256 ± 0.417
4.239SerVal: 4.239 ± 0.471
1.474SerTrp: 1.474 ± 0.308
1.29SerTyr: 1.29 ± 0.247
0.0SerXaa: 0.0 ± 0.0
Thr
5.651ThrAla: 5.651 ± 0.633
0.799ThrCys: 0.799 ± 0.233
3.501ThrAsp: 3.501 ± 0.451
4.73ThrGlu: 4.73 ± 0.471
2.15ThrPhe: 2.15 ± 0.366
5.099ThrGly: 5.099 ± 0.52
0.921ThrHis: 0.921 ± 0.234
3.379ThrIle: 3.379 ± 0.346
3.747ThrLys: 3.747 ± 0.553
5.529ThrLeu: 5.529 ± 0.529
1.597ThrMet: 1.597 ± 0.29
1.413ThrAsn: 1.413 ± 0.359
3.993ThrPro: 3.993 ± 0.507
2.396ThrGln: 2.396 ± 0.368
3.87ThrArg: 3.87 ± 0.602
3.563ThrSer: 3.563 ± 0.452
3.993ThrThr: 3.993 ± 0.577
3.747ThrVal: 3.747 ± 0.412
1.351ThrTrp: 1.351 ± 0.252
2.15ThrTyr: 2.15 ± 0.331
0.0ThrXaa: 0.0 ± 0.0
Val
5.836ValAla: 5.836 ± 0.641
0.86ValCys: 0.86 ± 0.253
6.02ValAsp: 6.02 ± 0.623
4.73ValGlu: 4.73 ± 0.585
1.966ValPhe: 1.966 ± 0.38
4.239ValGly: 4.239 ± 0.559
0.921ValHis: 0.921 ± 0.245
3.87ValIle: 3.87 ± 0.58
3.256ValLys: 3.256 ± 0.413
4.914ValLeu: 4.914 ± 0.695
0.983ValMet: 0.983 ± 0.279
3.071ValAsn: 3.071 ± 0.371
3.993ValPro: 3.993 ± 0.535
2.15ValGln: 2.15 ± 0.419
3.993ValArg: 3.993 ± 0.522
4.853ValSer: 4.853 ± 0.634
4.423ValThr: 4.423 ± 0.486
5.221ValVal: 5.221 ± 0.564
1.659ValTrp: 1.659 ± 0.319
2.396ValTyr: 2.396 ± 0.471
0.0ValXaa: 0.0 ± 0.0
Trp
1.843TrpAla: 1.843 ± 0.314
0.246TrpCys: 0.246 ± 0.114
1.843TrpAsp: 1.843 ± 0.309
1.536TrpGlu: 1.536 ± 0.263
0.676TrpPhe: 0.676 ± 0.175
0.983TrpGly: 0.983 ± 0.268
0.43TrpHis: 0.43 ± 0.145
1.413TrpIle: 1.413 ± 0.294
0.737TrpLys: 0.737 ± 0.261
0.799TrpLeu: 0.799 ± 0.216
0.43TrpMet: 0.43 ± 0.152
0.676TrpAsn: 0.676 ± 0.218
0.799TrpPro: 0.799 ± 0.231
1.167TrpGln: 1.167 ± 0.304
1.29TrpArg: 1.29 ± 0.259
1.29TrpSer: 1.29 ± 0.265
1.229TrpThr: 1.229 ± 0.271
0.983TrpVal: 0.983 ± 0.278
0.553TrpTrp: 0.553 ± 0.248
0.491TrpTyr: 0.491 ± 0.178
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.58TyrAla: 2.58 ± 0.497
0.061TyrCys: 0.061 ± 0.062
2.457TyrAsp: 2.457 ± 0.394
1.536TyrGlu: 1.536 ± 0.266
1.106TyrPhe: 1.106 ± 0.297
2.641TyrGly: 2.641 ± 0.353
0.491TyrHis: 0.491 ± 0.246
0.921TyrIle: 0.921 ± 0.209
1.413TyrLys: 1.413 ± 0.327
3.256TyrLeu: 3.256 ± 0.358
0.737TyrMet: 0.737 ± 0.214
1.106TyrAsn: 1.106 ± 0.318
1.536TyrPro: 1.536 ± 0.313
1.106TyrGln: 1.106 ± 0.263
2.396TyrArg: 2.396 ± 0.377
1.781TyrSer: 1.781 ± 0.373
2.211TyrThr: 2.211 ± 0.353
2.764TyrVal: 2.764 ± 0.498
0.676TyrTrp: 0.676 ± 0.2
1.044TyrTyr: 1.044 ± 0.241
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 101 proteins (16280 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski