Halolamina sp. CBA1230

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halolamina; unclassified Halolamina

Average proteome isoelectric point is 4.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3470 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5C3D1|A0A7D5C3D1_9EURY Sugar-specific transcriptional regulator TrmB OS=Halolamina sp. CBA1230 OX=1853690 GN=B4589_000410 PE=4 SV=1
MM1 pKa = 7.05TGPWAEE7 pKa = 3.87WDD9 pKa = 3.64HH10 pKa = 5.5VLKK13 pKa = 10.83VDD15 pKa = 3.53PDD17 pKa = 3.49KK18 pKa = 11.0EE19 pKa = 4.13LRR21 pKa = 11.84EE22 pKa = 4.37GEE24 pKa = 4.31TFEE27 pKa = 5.77DD28 pKa = 4.25VAATGTDD35 pKa = 3.09ALEE38 pKa = 4.31IGGTMDD44 pKa = 3.2VTSEE48 pKa = 3.53KK49 pKa = 9.03MQRR52 pKa = 11.84VVDD55 pKa = 3.98ACAAHH60 pKa = 6.99DD61 pKa = 3.74VPIYY65 pKa = 10.11QEE67 pKa = 3.88PSNPGVVIEE76 pKa = 4.73HH77 pKa = 7.36DD78 pKa = 3.79ALDD81 pKa = 4.75GYY83 pKa = 10.59LVPTVLNAGDD93 pKa = 4.26PFWITGAHH101 pKa = 6.22KK102 pKa = 9.33EE103 pKa = 3.86WARR106 pKa = 11.84IDD108 pKa = 5.9DD109 pKa = 4.46DD110 pKa = 5.56LDD112 pKa = 3.36WPNTHH117 pKa = 6.24TEE119 pKa = 4.2AYY121 pKa = 10.13VVLNPEE127 pKa = 4.24ASVAAYY133 pKa = 9.09TDD135 pKa = 3.78ADD137 pKa = 4.04CDD139 pKa = 3.74LTAEE143 pKa = 4.58DD144 pKa = 3.58VAAYY148 pKa = 10.27AGVAEE153 pKa = 4.72RR154 pKa = 11.84MFGQDD159 pKa = 2.44IVYY162 pKa = 10.51LEE164 pKa = 4.01YY165 pKa = 10.88SGTYY169 pKa = 10.14GDD171 pKa = 4.77PEE173 pKa = 4.34TVAAAAEE180 pKa = 4.1EE181 pKa = 4.54LDD183 pKa = 4.71DD184 pKa = 3.92ATLFYY189 pKa = 11.24GGGIHH194 pKa = 7.63DD195 pKa = 4.39YY196 pKa = 10.84EE197 pKa = 4.72SARR200 pKa = 11.84GMGEE204 pKa = 3.69HH205 pKa = 7.33ADD207 pKa = 3.84TVVVGDD213 pKa = 4.37LLHH216 pKa = 7.44DD217 pKa = 4.24EE218 pKa = 4.95GVDD221 pKa = 3.71AVAEE225 pKa = 4.35TVDD228 pKa = 3.69GAKK231 pKa = 10.12EE232 pKa = 3.64AA233 pKa = 4.21

Molecular weight: 25.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D4Y5G0|A0A7D4Y5G0_9EURY DNA-directed RNA polymerase subunit H OS=Halolamina sp. CBA1230 OX=1853690 GN=rpoH PE=3 SV=1
MM1 pKa = 7.36PTLEE5 pKa = 4.2TWSLWLHH12 pKa = 6.13IAAGTVAVLAGVGALVTKK30 pKa = 10.59KK31 pKa = 10.14GGRR34 pKa = 11.84RR35 pKa = 11.84HH36 pKa = 6.32RR37 pKa = 11.84GAGKK41 pKa = 10.43LFLAAMAVTVATVFVLLAAAVTTFRR66 pKa = 11.84IVLTLVAVFSGYY78 pKa = 10.61LAFSGYY84 pKa = 10.05RR85 pKa = 11.84VLSRR89 pKa = 11.84KK90 pKa = 9.72RR91 pKa = 11.84PSEE94 pKa = 4.1TPATVDD100 pKa = 2.74WLAAGTVVVACLVLGSWGVAWFLDD124 pKa = 4.17GSSFGVVMAVFGGIGVAFGGQEE146 pKa = 3.58LRR148 pKa = 11.84SFRR151 pKa = 11.84RR152 pKa = 11.84GEE154 pKa = 3.92SGEE157 pKa = 3.99WLVGHH162 pKa = 6.64LQRR165 pKa = 11.84MIAAFIATISAVSAVNLTPVIGVSAWLWPTVVGTPLIAYY204 pKa = 7.72WSRR207 pKa = 11.84KK208 pKa = 8.58YY209 pKa = 10.8EE210 pKa = 3.93SDD212 pKa = 3.13RR213 pKa = 4.13

Molecular weight: 22.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3470

0

3470

987246

39

2112

284.5

30.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.252 ± 0.069

0.69 ± 0.013

8.345 ± 0.048

8.996 ± 0.067

3.27 ± 0.028

8.666 ± 0.038

1.982 ± 0.019

3.743 ± 0.03

1.779 ± 0.028

8.993 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.676 ± 0.017

2.295 ± 0.027

4.744 ± 0.027

2.502 ± 0.024

6.517 ± 0.053

5.413 ± 0.031

6.234 ± 0.039

9.035 ± 0.047

1.174 ± 0.015

2.694 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski