Amino acid dipepetide frequency for Flavobacteriaceae bacterium PLHSN227

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.708AlaAla: 4.708 ± 0.094	0.619AlaCys: 0.619 ± 0.03	3.239AlaAsp: 3.239 ± 0.06	4.779AlaGlu: 4.779 ± 0.082	3.548AlaPhe: 3.548 ± 0.066	4.381AlaGly: 4.381 ± 0.089	1.145AlaHis: 1.145 ± 0.04	5.483AlaIle: 5.483 ± 0.085	5.576AlaLys: 5.576 ± 0.088	6.628AlaLeu: 6.628 ± 0.102	1.576AlaMet: 1.576 ± 0.047	3.737AlaAsn: 3.737 ± 0.082	1.934AlaPro: 1.934 ± 0.049	2.858AlaGln: 2.858 ± 0.063	2.02AlaArg: 2.02 ± 0.051	3.976AlaSer: 3.976 ± 0.071	3.682AlaThr: 3.682 ± 0.088	4.44AlaVal: 4.44 ± 0.072	0.605AlaTrp: 0.605 ± 0.026	2.75AlaTyr: 2.75 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.44CysAla: 0.44 ± 0.026	0.081CysCys: 0.081 ± 0.011	0.397CysAsp: 0.397 ± 0.023	0.39CysGlu: 0.39 ± 0.024	0.378CysPhe: 0.378 ± 0.022	0.582CysGly: 0.582 ± 0.027	0.157CysHis: 0.157 ± 0.017	0.519CysIle: 0.519 ± 0.028	0.488CysLys: 0.488 ± 0.026	0.598CysLeu: 0.598 ± 0.027	0.121CysMet: 0.121 ± 0.012	0.375CysAsn: 0.375 ± 0.021	0.315CysPro: 0.315 ± 0.025	0.259CysGln: 0.259 ± 0.02	0.169CysArg: 0.169 ± 0.015	0.496CysSer: 0.496 ± 0.029	0.364CysThr: 0.364 ± 0.021	0.436CysVal: 0.436 ± 0.025	0.065CysTrp: 0.065 ± 0.011	0.265CysTyr: 0.265 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	3.851AspAla: 3.851 ± 0.077	0.392AspCys: 0.392 ± 0.023	2.531AspAsp: 2.531 ± 0.076	3.343AspGlu: 3.343 ± 0.065	3.802AspPhe: 3.802 ± 0.066	3.209AspGly: 3.209 ± 0.085	0.738AspHis: 0.738 ± 0.026	3.843AspIle: 3.843 ± 0.06	4.428AspLys: 4.428 ± 0.082	5.334AspLeu: 5.334 ± 0.081	0.947AspMet: 0.947 ± 0.035	3.036AspAsn: 3.036 ± 0.078	1.606AspPro: 1.606 ± 0.048	1.512AspGln: 1.512 ± 0.04	1.742AspArg: 1.742 ± 0.046	2.891AspSer: 2.891 ± 0.071	2.605AspThr: 2.605 ± 0.059	3.299AspVal: 3.299 ± 0.072	0.696AspTrp: 0.696 ± 0.032	2.903AspTyr: 2.903 ± 0.07	0.0AspXaa: 0.0 ± 0.0
Glu	4.824GluAla: 4.824 ± 0.078	0.299GluCys: 0.299 ± 0.02	4.113GluAsp: 4.113 ± 0.082	6.447GluGlu: 6.447 ± 0.113	2.968GluPhe: 2.968 ± 0.064	3.689GluGly: 3.689 ± 0.079	1.19GluHis: 1.19 ± 0.042	5.995GluIle: 5.995 ± 0.088	7.351GluLys: 7.351 ± 0.111	5.906GluLeu: 5.906 ± 0.096	1.654GluMet: 1.654 ± 0.044	5.604GluAsn: 5.604 ± 0.102	1.73GluPro: 1.73 ± 0.053	2.556GluGln: 2.556 ± 0.058	2.551GluArg: 2.551 ± 0.06	3.251GluSer: 3.251 ± 0.064	3.789GluThr: 3.789 ± 0.074	4.723GluVal: 4.723 ± 0.082	0.616GluTrp: 0.616 ± 0.029	2.332GluTyr: 2.332 ± 0.053	0.0GluXaa: 0.0 ± 0.0
Phe	3.179PheAla: 3.179 ± 0.067	0.466PheCys: 0.466 ± 0.028	2.975PheAsp: 2.975 ± 0.062	3.431PheGlu: 3.431 ± 0.066	2.736PhePhe: 2.736 ± 0.071	3.51PheGly: 3.51 ± 0.073	0.758PheHis: 0.758 ± 0.032	3.886PheIle: 3.886 ± 0.069	4.028PheLys: 4.028 ± 0.082	5.216PheLeu: 5.216 ± 0.103	1.064PheMet: 1.064 ± 0.036	2.906PheAsn: 2.906 ± 0.073	1.8PhePro: 1.8 ± 0.05	1.618PheGln: 1.618 ± 0.044	1.697PheArg: 1.697 ± 0.051	4.022PheSer: 4.022 ± 0.074	3.229PheThr: 3.229 ± 0.066	2.945PheVal: 2.945 ± 0.053	0.616PheTrp: 0.616 ± 0.028	2.336PheTyr: 2.336 ± 0.06	0.0PheXaa: 0.0 ± 0.0
Gly	4.05GlyAla: 4.05 ± 0.092	0.512GlyCys: 0.512 ± 0.023	3.168GlyAsp: 3.168 ± 0.069	3.884GlyGlu: 3.884 ± 0.073	3.622GlyPhe: 3.622 ± 0.072	4.174GlyGly: 4.174 ± 0.089	1.022GlyHis: 1.022 ± 0.03	5.049GlyIle: 5.049 ± 0.087	5.261GlyLys: 5.261 ± 0.095	5.65GlyLeu: 5.65 ± 0.092	1.528GlyMet: 1.528 ± 0.045	3.511GlyAsn: 3.511 ± 0.073	1.323GlyPro: 1.323 ± 0.048	1.898GlyGln: 1.898 ± 0.048	1.999GlyArg: 1.999 ± 0.057	3.841GlySer: 3.841 ± 0.084	3.364GlyThr: 3.364 ± 0.102	3.973GlyVal: 3.973 ± 0.083	0.651GlyTrp: 0.651 ± 0.027	2.608GlyTyr: 2.608 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	0.96HisAla: 0.96 ± 0.037	0.175HisCys: 0.175 ± 0.015	0.718HisAsp: 0.718 ± 0.032	0.961HisGlu: 0.961 ± 0.035	1.134HisPhe: 1.134 ± 0.041	0.925HisGly: 0.925 ± 0.036	0.407HisHis: 0.407 ± 0.026	1.304HisIle: 1.304 ± 0.042	1.339HisLys: 1.339 ± 0.045	1.898HisLeu: 1.898 ± 0.052	0.256HisMet: 0.256 ± 0.016	0.892HisAsn: 0.892 ± 0.036	0.899HisPro: 0.899 ± 0.036	0.771HisGln: 0.771 ± 0.037	0.637HisArg: 0.637 ± 0.025	0.979HisSer: 0.979 ± 0.038	0.929HisThr: 0.929 ± 0.032	0.818HisVal: 0.818 ± 0.034	0.182HisTrp: 0.182 ± 0.013	0.903HisTyr: 0.903 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.706IleAla: 5.706 ± 0.095	0.557IleCys: 0.557 ± 0.027	4.441IleAsp: 4.441 ± 0.071	5.572IleGlu: 5.572 ± 0.086	3.635IlePhe: 3.635 ± 0.069	4.991IleGly: 4.991 ± 0.096	1.09IleHis: 1.09 ± 0.037	5.413IleIle: 5.413 ± 0.098	5.876IleLys: 5.876 ± 0.077	7.035IleLeu: 7.035 ± 0.11	1.256IleMet: 1.256 ± 0.042	4.449IleAsn: 4.449 ± 0.073	3.139IlePro: 3.139 ± 0.061	2.483IleGln: 2.483 ± 0.052	2.556IleArg: 2.556 ± 0.047	4.914IleSer: 4.914 ± 0.076	4.281IleThr: 4.281 ± 0.09	4.222IleVal: 4.222 ± 0.09	0.667IleTrp: 0.667 ± 0.03	2.77IleTyr: 2.77 ± 0.06	0.0IleXaa: 0.0 ± 0.0
Lys	5.667LysAla: 5.667 ± 0.099	0.311LysCys: 0.311 ± 0.021	4.817LysAsp: 4.817 ± 0.082	7.47LysGlu: 7.47 ± 0.122	3.098LysPhe: 3.098 ± 0.062	4.048LysGly: 4.048 ± 0.085	1.605LysHis: 1.605 ± 0.055	6.587LysIle: 6.587 ± 0.096	8.369LysLys: 8.369 ± 0.124	7.23LysLeu: 7.23 ± 0.097	2.093LysMet: 2.093 ± 0.056	6.099LysAsn: 6.099 ± 0.102	2.775LysPro: 2.775 ± 0.055	3.355LysGln: 3.355 ± 0.076	3.32LysArg: 3.32 ± 0.07	4.342LysSer: 4.342 ± 0.083	5.102LysThr: 5.102 ± 0.095	4.731LysVal: 4.731 ± 0.078	0.725LysTrp: 0.725 ± 0.03	2.892LysTyr: 2.892 ± 0.061	0.0LysXaa: 0.0 ± 0.0
Leu	6.433LeuAla: 6.433 ± 0.107	0.609LeuCys: 0.609 ± 0.027	5.136LeuAsp: 5.136 ± 0.083	7.039LeuGlu: 7.039 ± 0.093	4.838LeuPhe: 4.838 ± 0.089	5.72LeuGly: 5.72 ± 0.098	1.735LeuHis: 1.735 ± 0.044	6.578LeuIle: 6.578 ± 0.099	7.894LeuLys: 7.894 ± 0.118	9.443LeuLeu: 9.443 ± 0.157	1.929LeuMet: 1.929 ± 0.054	5.489LeuAsn: 5.489 ± 0.089	3.83LeuPro: 3.83 ± 0.058	4.416LeuGln: 4.416 ± 0.083	3.459LeuArg: 3.459 ± 0.07	6.761LeuSer: 6.761 ± 0.095	4.801LeuThr: 4.801 ± 0.083	5.549LeuVal: 5.549 ± 0.093	0.777LeuTrp: 0.777 ± 0.033	3.361LeuTyr: 3.361 ± 0.071	0.0LeuXaa: 0.0 ± 0.0
Met	1.675MetAla: 1.675 ± 0.046	0.117MetCys: 0.117 ± 0.011	0.876MetAsp: 0.876 ± 0.036	1.273MetGlu: 1.273 ± 0.04	0.818MetPhe: 0.818 ± 0.039	1.371MetGly: 1.371 ± 0.043	0.449MetHis: 0.449 ± 0.026	1.173MetIle: 1.173 ± 0.043	1.894MetLys: 1.894 ± 0.048	2.131MetLeu: 2.131 ± 0.057	0.508MetMet: 0.508 ± 0.027	1.062MetAsn: 1.062 ± 0.037	0.826MetPro: 0.826 ± 0.034	1.231MetGln: 1.231 ± 0.04	1.034MetArg: 1.034 ± 0.035	1.175MetSer: 1.175 ± 0.038	0.939MetThr: 0.939 ± 0.034	1.321MetVal: 1.321 ± 0.038	0.148MetTrp: 0.148 ± 0.013	0.599MetTyr: 0.599 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	4.148AsnAla: 4.148 ± 0.071	0.471AsnCys: 0.471 ± 0.027	2.68AsnAsp: 2.68 ± 0.06	3.298AsnGlu: 3.298 ± 0.07	3.657AsnPhe: 3.657 ± 0.084	3.578AsnGly: 3.578 ± 0.088	0.988AsnHis: 0.988 ± 0.036	4.502AsnIle: 4.502 ± 0.076	4.727AsnLys: 4.727 ± 0.083	6.425AsnLeu: 6.425 ± 0.126	1.124AsnMet: 1.124 ± 0.039	3.775AsnAsn: 3.775 ± 0.083	2.886AsnPro: 2.886 ± 0.062	2.704AsnGln: 2.704 ± 0.06	2.226AsnArg: 2.226 ± 0.047	3.465AsnSer: 3.465 ± 0.072	3.493AsnThr: 3.493 ± 0.076	3.078AsnVal: 3.078 ± 0.075	0.854AsnTrp: 0.854 ± 0.041	3.069AsnTyr: 3.069 ± 0.076	0.0AsnXaa: 0.0 ± 0.0
Pro	2.054ProAla: 2.054 ± 0.055	0.207ProCys: 0.207 ± 0.016	1.975ProAsp: 1.975 ± 0.051	3.002ProGlu: 3.002 ± 0.064	1.791ProPhe: 1.791 ± 0.05	2.036ProGly: 2.036 ± 0.045	0.614ProHis: 0.614 ± 0.028	2.632ProIle: 2.632 ± 0.057	2.878ProLys: 2.878 ± 0.074	2.864ProLeu: 2.864 ± 0.072	0.633ProMet: 0.633 ± 0.03	2.165ProAsn: 2.165 ± 0.058	0.913ProPro: 0.913 ± 0.038	1.481ProGln: 1.481 ± 0.046	0.944ProArg: 0.944 ± 0.036	1.971ProSer: 1.971 ± 0.057	1.937ProThr: 1.937 ± 0.055	2.426ProVal: 2.426 ± 0.058	0.301ProTrp: 0.301 ± 0.02	1.401ProTyr: 1.401 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	2.703GlnAla: 2.703 ± 0.052	0.16GlnCys: 0.16 ± 0.013	2.001GlnAsp: 2.001 ± 0.05	3.48GlnGlu: 3.48 ± 0.068	1.639GlnPhe: 1.639 ± 0.052	1.814GlnGly: 1.814 ± 0.054	0.666GlnHis: 0.666 ± 0.03	2.775GlnIle: 2.775 ± 0.056	3.705GlnLys: 3.705 ± 0.08	3.82GlnLeu: 3.82 ± 0.067	0.835GlnMet: 0.835 ± 0.032	2.697GlnAsn: 2.697 ± 0.06	1.287GlnPro: 1.287 ± 0.04	1.934GlnGln: 1.934 ± 0.058	1.373GlnArg: 1.373 ± 0.04	1.895GlnSer: 1.895 ± 0.053	2.112GlnThr: 2.112 ± 0.051	2.249GlnVal: 2.249 ± 0.044	0.35GlnTrp: 0.35 ± 0.02	1.368GlnTyr: 1.368 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	2.087ArgAla: 2.087 ± 0.056	0.179ArgCys: 0.179 ± 0.014	1.797ArgAsp: 1.797 ± 0.049	2.533ArgGlu: 2.533 ± 0.063	1.924ArgPhe: 1.924 ± 0.056	1.922ArgGly: 1.922 ± 0.053	0.602ArgHis: 0.602 ± 0.031	2.743ArgIle: 2.743 ± 0.054	3.344ArgLys: 3.344 ± 0.074	3.523ArgLeu: 3.523 ± 0.069	0.835ArgMet: 0.835 ± 0.031	2.097ArgAsn: 2.097 ± 0.056	1.026ArgPro: 1.026 ± 0.034	1.277ArgGln: 1.277 ± 0.042	1.328ArgArg: 1.328 ± 0.05	1.799ArgSer: 1.799 ± 0.051	1.571ArgThr: 1.571 ± 0.047	2.1ArgVal: 2.1 ± 0.049	0.347ArgTrp: 0.347 ± 0.021	1.572ArgTyr: 1.572 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	3.914SerAla: 3.914 ± 0.069	0.5SerCys: 0.5 ± 0.024	2.729SerAsp: 2.729 ± 0.067	3.535SerGlu: 3.535 ± 0.072	3.717SerPhe: 3.717 ± 0.075	4.282SerGly: 4.282 ± 0.086	0.898SerHis: 0.898 ± 0.032	4.866SerIle: 4.866 ± 0.081	4.723SerLys: 4.723 ± 0.084	6.142SerLeu: 6.142 ± 0.106	1.17SerMet: 1.17 ± 0.037	3.354SerAsn: 3.354 ± 0.084	1.943SerPro: 1.943 ± 0.051	2.026SerGln: 2.026 ± 0.047	1.901SerArg: 1.901 ± 0.048	3.739SerSer: 3.739 ± 0.075	3.261SerThr: 3.261 ± 0.076	3.986SerVal: 3.986 ± 0.077	0.632SerTrp: 0.632 ± 0.028	2.62SerTyr: 2.62 ± 0.065	0.0SerXaa: 0.0 ± 0.0
Thr	4.062ThrAla: 4.062 ± 0.085	0.371ThrCys: 0.371 ± 0.025	2.996ThrAsp: 2.996 ± 0.075	3.76ThrGlu: 3.76 ± 0.085	2.624ThrPhe: 2.624 ± 0.058	3.926ThrGly: 3.926 ± 0.091	1.006ThrHis: 1.006 ± 0.037	3.989ThrIle: 3.989 ± 0.073	4.014ThrLys: 4.014 ± 0.071	4.843ThrLeu: 4.843 ± 0.082	0.89ThrMet: 0.89 ± 0.032	3.231ThrAsn: 3.231 ± 0.084	2.288ThrPro: 2.288 ± 0.059	2.2ThrGln: 2.2 ± 0.051	1.616ThrArg: 1.616 ± 0.045	3.412ThrSer: 3.412 ± 0.069	3.272ThrThr: 3.272 ± 0.104	3.246ThrVal: 3.246 ± 0.064	0.516ThrTrp: 0.516 ± 0.026	2.394ThrTyr: 2.394 ± 0.065	0.001ThrXaa: 0.001 ± 0.001
Val	4.014ValAla: 4.014 ± 0.074	0.5ValCys: 0.5 ± 0.026	3.37ValAsp: 3.37 ± 0.075	4.277ValGlu: 4.277 ± 0.08	3.419ValPhe: 3.419 ± 0.073	3.881ValGly: 3.881 ± 0.076	0.984ValHis: 0.984 ± 0.033	4.454ValIle: 4.454 ± 0.083	4.732ValLys: 4.732 ± 0.084	6.01ValLeu: 6.01 ± 0.113	1.229ValMet: 1.229 ± 0.041	3.583ValAsn: 3.583 ± 0.07	2.001ValPro: 2.001 ± 0.051	1.933ValGln: 1.933 ± 0.045	1.922ValArg: 1.922 ± 0.049	3.976ValSer: 3.976 ± 0.071	3.049ValThr: 3.049 ± 0.073	4.015ValVal: 4.015 ± 0.1	0.536ValTrp: 0.536 ± 0.026	2.371ValTyr: 2.371 ± 0.065	0.0ValXaa: 0.0 ± 0.0
Trp	0.565TrpAla: 0.565 ± 0.026	0.076TrpCys: 0.076 ± 0.01	0.555TrpAsp: 0.555 ± 0.027	0.702TrpGlu: 0.702 ± 0.028	0.581TrpPhe: 0.581 ± 0.027	0.534TrpGly: 0.534 ± 0.026	0.232TrpHis: 0.232 ± 0.018	0.623TrpIle: 0.623 ± 0.029	0.716TrpLys: 0.716 ± 0.03	1.064TrpLeu: 1.064 ± 0.037	0.296TrpMet: 0.296 ± 0.019	0.617TrpAsn: 0.617 ± 0.03	0.245TrpPro: 0.245 ± 0.019	0.533TrpGln: 0.533 ± 0.026	0.368TrpArg: 0.368 ± 0.022	0.555TrpSer: 0.555 ± 0.026	0.458TrpThr: 0.458 ± 0.028	0.6TrpVal: 0.6 ± 0.032	0.136TrpTrp: 0.136 ± 0.016	0.403TrpTyr: 0.403 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.642TyrAla: 2.642 ± 0.063	0.297TyrCys: 0.297 ± 0.02	2.194TyrAsp: 2.194 ± 0.053	2.412TyrGlu: 2.412 ± 0.054	2.488TyrPhe: 2.488 ± 0.056	2.516TyrGly: 2.516 ± 0.062	0.803TyrHis: 0.803 ± 0.032	2.64TyrIle: 2.64 ± 0.058	3.293TyrLys: 3.293 ± 0.068	4.037TyrLeu: 4.037 ± 0.068	0.674TyrMet: 0.674 ± 0.026	2.469TyrAsn: 2.469 ± 0.066	1.455TyrPro: 1.455 ± 0.042	1.819TyrGln: 1.819 ± 0.052	1.746TyrArg: 1.746 ± 0.047	2.478TyrSer: 2.478 ± 0.062	2.418TyrThr: 2.418 ± 0.07	2.098TyrVal: 2.098 ± 0.048	0.437TyrTrp: 0.437 ± 0.021	1.952TyrTyr: 1.952 ± 0.055	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2578 proteins (856065 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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