Amino acid dipepetide frequency for Gordonia phage Bantam

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.174AlaAla: 14.174 ± 1.366
0.589AlaCys: 0.589 ± 0.15
8.525AlaAsp: 8.525 ± 0.532
7.728AlaGlu: 7.728 ± 0.736
2.946AlaPhe: 2.946 ± 0.305
8.941AlaGly: 8.941 ± 0.757
1.698AlaHis: 1.698 ± 0.26
5.302AlaIle: 5.302 ± 0.417
4.367AlaLys: 4.367 ± 0.455
8.699AlaLeu: 8.699 ± 0.72
3.466AlaMet: 3.466 ± 0.282
3.119AlaAsn: 3.119 ± 0.418
4.159AlaPro: 4.159 ± 0.352
4.471AlaGln: 4.471 ± 0.609
6.758AlaArg: 6.758 ± 0.581
6.03AlaSer: 6.03 ± 0.518
6.134AlaThr: 6.134 ± 0.632
6.862AlaVal: 6.862 ± 0.452
1.629AlaTrp: 1.629 ± 0.225
2.495AlaTyr: 2.495 ± 0.239
0.0AlaXaa: 0.0 ± 0.0
Cys
1.109CysAla: 1.109 ± 0.26
0.173CysCys: 0.173 ± 0.087
0.589CysAsp: 0.589 ± 0.158
0.416CysGlu: 0.416 ± 0.137
0.208CysPhe: 0.208 ± 0.092
1.109CysGly: 1.109 ± 0.207
0.381CysHis: 0.381 ± 0.143
0.416CysIle: 0.416 ± 0.129
0.139CysLys: 0.139 ± 0.069
0.554CysLeu: 0.554 ± 0.159
0.243CysMet: 0.243 ± 0.084
0.243CysAsn: 0.243 ± 0.1
0.589CysPro: 0.589 ± 0.153
0.277CysGln: 0.277 ± 0.11
0.762CysArg: 0.762 ± 0.195
0.347CysSer: 0.347 ± 0.115
0.416CysThr: 0.416 ± 0.14
0.416CysVal: 0.416 ± 0.111
0.069CysTrp: 0.069 ± 0.045
0.104CysTyr: 0.104 ± 0.063
0.0CysXaa: 0.0 ± 0.0
Asp
7.001AspAla: 7.001 ± 0.575
0.693AspCys: 0.693 ± 0.18
4.644AspAsp: 4.644 ± 0.579
6.169AspGlu: 6.169 ± 0.671
2.599AspPhe: 2.599 ± 0.326
6.203AspGly: 6.203 ± 0.464
1.594AspHis: 1.594 ± 0.233
4.02AspIle: 4.02 ± 0.393
2.183AspLys: 2.183 ± 0.293
6.134AspLeu: 6.134 ± 0.424
1.594AspMet: 1.594 ± 0.214
2.53AspAsn: 2.53 ± 0.282
4.228AspPro: 4.228 ± 0.389
1.871AspGln: 1.871 ± 0.25
5.337AspArg: 5.337 ± 0.47
3.362AspSer: 3.362 ± 0.313
3.466AspThr: 3.466 ± 0.325
5.337AspVal: 5.337 ± 0.529
2.079AspTrp: 2.079 ± 0.318
1.941AspTyr: 1.941 ± 0.291
0.0AspXaa: 0.0 ± 0.0
Glu
7.867GluAla: 7.867 ± 0.7
0.451GluCys: 0.451 ± 0.154
4.505GluAsp: 4.505 ± 0.426
3.985GluGlu: 3.985 ± 0.392
2.946GluPhe: 2.946 ± 0.372
5.164GluGly: 5.164 ± 0.565
1.663GluHis: 1.663 ± 0.283
4.193GluIle: 4.193 ± 0.491
2.634GluLys: 2.634 ± 0.32
5.857GluLeu: 5.857 ± 0.523
1.767GluMet: 1.767 ± 0.251
2.079GluAsn: 2.079 ± 0.295
2.426GluPro: 2.426 ± 0.334
2.807GluGln: 2.807 ± 0.33
4.436GluArg: 4.436 ± 0.438
3.188GluSer: 3.188 ± 0.344
3.847GluThr: 3.847 ± 0.452
5.268GluVal: 5.268 ± 0.469
1.941GluTrp: 1.941 ± 0.289
2.183GluTyr: 2.183 ± 0.355
0.0GluXaa: 0.0 ± 0.0
Phe
3.223PheAla: 3.223 ± 0.296
0.485PheCys: 0.485 ± 0.133
3.015PheAsp: 3.015 ± 0.371
2.114PheGlu: 2.114 ± 0.26
0.797PhePhe: 0.797 ± 0.174
2.669PheGly: 2.669 ± 0.329
0.832PheHis: 0.832 ± 0.226
1.386PheIle: 1.386 ± 0.267
0.97PheLys: 0.97 ± 0.184
1.941PheLeu: 1.941 ± 0.326
1.213PheMet: 1.213 ± 0.202
1.144PheAsn: 1.144 ± 0.183
1.248PhePro: 1.248 ± 0.212
0.797PheGln: 0.797 ± 0.163
2.01PheArg: 2.01 ± 0.259
1.629PheSer: 1.629 ± 0.266
2.495PheThr: 2.495 ± 0.324
1.56PheVal: 1.56 ± 0.27
0.624PheTrp: 0.624 ± 0.144
0.728PheTyr: 0.728 ± 0.137
0.0PheXaa: 0.0 ± 0.0
Gly
6.862GlyAla: 6.862 ± 0.613
0.658GlyCys: 0.658 ± 0.159
5.684GlyAsp: 5.684 ± 0.539
6.169GlyGlu: 6.169 ± 0.503
2.738GlyPhe: 2.738 ± 0.332
7.555GlyGly: 7.555 ± 0.745
1.837GlyHis: 1.837 ± 0.216
3.847GlyIle: 3.847 ± 0.409
3.466GlyLys: 3.466 ± 0.368
6.723GlyLeu: 6.723 ± 0.515
1.906GlyMet: 1.906 ± 0.222
2.842GlyAsn: 2.842 ± 0.423
3.258GlyPro: 3.258 ± 0.333
2.842GlyGln: 2.842 ± 0.359
6.169GlyArg: 6.169 ± 0.44
4.436GlySer: 4.436 ± 0.396
4.401GlyThr: 4.401 ± 0.42
5.372GlyVal: 5.372 ± 0.441
1.525GlyTrp: 1.525 ± 0.192
2.946GlyTyr: 2.946 ± 0.309
0.0GlyXaa: 0.0 ± 0.0
His
1.525HisAla: 1.525 ± 0.291
0.381HisCys: 0.381 ± 0.112
1.456HisAsp: 1.456 ± 0.262
1.456HisGlu: 1.456 ± 0.268
0.624HisPhe: 0.624 ± 0.153
1.282HisGly: 1.282 ± 0.191
0.589HisHis: 0.589 ± 0.143
1.421HisIle: 1.421 ± 0.244
0.381HisLys: 0.381 ± 0.113
2.53HisLeu: 2.53 ± 0.258
0.52HisMet: 0.52 ± 0.129
0.416HisAsn: 0.416 ± 0.131
1.317HisPro: 1.317 ± 0.224
0.728HisGln: 0.728 ± 0.127
1.248HisArg: 1.248 ± 0.222
1.248HisSer: 1.248 ± 0.225
1.248HisThr: 1.248 ± 0.221
1.525HisVal: 1.525 ± 0.271
0.589HisTrp: 0.589 ± 0.15
0.866HisTyr: 0.866 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
6.238IleAla: 6.238 ± 0.473
0.485IleCys: 0.485 ± 0.13
4.54IleAsp: 4.54 ± 0.376
4.817IleGlu: 4.817 ± 0.504
1.525IlePhe: 1.525 ± 0.225
3.985IleGly: 3.985 ± 0.518
1.04IleHis: 1.04 ± 0.217
2.322IleIle: 2.322 ± 0.326
1.906IleLys: 1.906 ± 0.259
3.154IleLeu: 3.154 ± 0.334
1.144IleMet: 1.144 ± 0.182
1.56IleAsn: 1.56 ± 0.244
2.218IlePro: 2.218 ± 0.264
1.594IleGln: 1.594 ± 0.215
4.575IleArg: 4.575 ± 0.348
2.599IleSer: 2.599 ± 0.314
3.119IleThr: 3.119 ± 0.343
3.327IleVal: 3.327 ± 0.46
1.04IleTrp: 1.04 ± 0.196
1.802IleTyr: 1.802 ± 0.254
0.0IleXaa: 0.0 ± 0.0
Lys
4.367LysAla: 4.367 ± 0.598
0.139LysCys: 0.139 ± 0.086
2.218LysAsp: 2.218 ± 0.353
1.56LysGlu: 1.56 ± 0.248
1.317LysPhe: 1.317 ± 0.197
2.807LysGly: 2.807 ± 0.412
0.589LysHis: 0.589 ± 0.168
2.565LysIle: 2.565 ± 0.269
1.767LysLys: 1.767 ± 0.286
3.154LysLeu: 3.154 ± 0.394
0.901LysMet: 0.901 ± 0.173
1.144LysAsn: 1.144 ± 0.18
2.322LysPro: 2.322 ± 0.318
0.797LysGln: 0.797 ± 0.147
3.084LysArg: 3.084 ± 0.349
2.426LysSer: 2.426 ± 0.326
2.114LysThr: 2.114 ± 0.296
2.53LysVal: 2.53 ± 0.314
0.589LysTrp: 0.589 ± 0.125
1.074LysTyr: 1.074 ± 0.168
0.0LysXaa: 0.0 ± 0.0
Leu
8.491LeuAla: 8.491 ± 0.648
0.832LeuCys: 0.832 ± 0.196
6.411LeuAsp: 6.411 ± 0.351
4.748LeuGlu: 4.748 ± 0.403
2.01LeuPhe: 2.01 ± 0.336
6.065LeuGly: 6.065 ± 0.483
1.698LeuHis: 1.698 ± 0.265
4.263LeuIle: 4.263 ± 0.307
2.599LeuLys: 2.599 ± 0.291
5.926LeuLeu: 5.926 ± 0.529
1.248LeuMet: 1.248 ± 0.182
2.634LeuAsn: 2.634 ± 0.263
4.471LeuPro: 4.471 ± 0.318
2.876LeuGln: 2.876 ± 0.323
5.961LeuArg: 5.961 ± 0.452
4.817LeuSer: 4.817 ± 0.418
5.164LeuThr: 5.164 ± 0.5
4.609LeuVal: 4.609 ± 0.433
1.248LeuTrp: 1.248 ± 0.227
1.975LeuTyr: 1.975 ± 0.23
0.0LeuXaa: 0.0 ± 0.0
Met
3.292MetAla: 3.292 ± 0.33
0.347MetCys: 0.347 ± 0.11
1.317MetAsp: 1.317 ± 0.207
1.317MetGlu: 1.317 ± 0.207
0.866MetPhe: 0.866 ± 0.2
1.49MetGly: 1.49 ± 0.224
0.693MetHis: 0.693 ± 0.156
0.936MetIle: 0.936 ± 0.21
1.04MetLys: 1.04 ± 0.192
1.56MetLeu: 1.56 ± 0.243
0.312MetMet: 0.312 ± 0.094
0.866MetAsn: 0.866 ± 0.222
1.386MetPro: 1.386 ± 0.188
0.624MetGln: 0.624 ± 0.16
1.386MetArg: 1.386 ± 0.233
2.079MetSer: 2.079 ± 0.274
2.426MetThr: 2.426 ± 0.27
1.698MetVal: 1.698 ± 0.238
0.243MetTrp: 0.243 ± 0.088
0.381MetTyr: 0.381 ± 0.125
0.0MetXaa: 0.0 ± 0.0
Asn
3.674AsnAla: 3.674 ± 0.499
0.139AsnCys: 0.139 ± 0.062
1.837AsnAsp: 1.837 ± 0.252
1.767AsnGlu: 1.767 ± 0.198
0.832AsnPhe: 0.832 ± 0.157
2.599AsnGly: 2.599 ± 0.369
0.728AsnHis: 0.728 ± 0.177
1.629AsnIle: 1.629 ± 0.202
1.213AsnLys: 1.213 ± 0.205
2.738AsnLeu: 2.738 ± 0.3
0.762AsnMet: 0.762 ± 0.173
1.074AsnAsn: 1.074 ± 0.199
2.426AsnPro: 2.426 ± 0.318
1.005AsnGln: 1.005 ± 0.195
2.772AsnArg: 2.772 ± 0.288
1.594AsnSer: 1.594 ± 0.293
1.56AsnThr: 1.56 ± 0.236
2.183AsnVal: 2.183 ± 0.325
0.554AsnTrp: 0.554 ± 0.112
0.728AsnTyr: 0.728 ± 0.151
0.0AsnXaa: 0.0 ± 0.0
Pro
5.614ProAla: 5.614 ± 0.434
0.52ProCys: 0.52 ± 0.159
4.193ProAsp: 4.193 ± 0.439
3.812ProGlu: 3.812 ± 0.413
1.56ProPhe: 1.56 ± 0.262
4.644ProGly: 4.644 ± 0.399
1.213ProHis: 1.213 ± 0.236
2.287ProIle: 2.287 ± 0.281
2.01ProLys: 2.01 ± 0.286
2.634ProLeu: 2.634 ± 0.312
1.04ProMet: 1.04 ± 0.173
1.663ProAsn: 1.663 ± 0.235
2.461ProPro: 2.461 ± 0.306
0.866ProGln: 0.866 ± 0.164
2.599ProArg: 2.599 ± 0.35
2.98ProSer: 2.98 ± 0.336
3.188ProThr: 3.188 ± 0.341
3.5ProVal: 3.5 ± 0.35
0.901ProTrp: 0.901 ± 0.178
1.282ProTyr: 1.282 ± 0.229
0.0ProXaa: 0.0 ± 0.0
Gln
3.674GlnAla: 3.674 ± 0.613
0.208GlnCys: 0.208 ± 0.085
1.698GlnAsp: 1.698 ± 0.242
2.114GlnGlu: 2.114 ± 0.477
1.213GlnPhe: 1.213 ± 0.249
2.322GlnGly: 2.322 ± 0.321
0.485GlnHis: 0.485 ± 0.13
2.426GlnIle: 2.426 ± 0.299
1.248GlnLys: 1.248 ± 0.196
3.258GlnLeu: 3.258 ± 0.572
0.832GlnMet: 0.832 ± 0.17
0.693GlnAsn: 0.693 ± 0.157
1.282GlnPro: 1.282 ± 0.216
1.386GlnGln: 1.386 ± 0.261
2.495GlnArg: 2.495 ± 0.282
1.49GlnSer: 1.49 ± 0.202
1.594GlnThr: 1.594 ± 0.23
2.461GlnVal: 2.461 ± 0.282
0.797GlnTrp: 0.797 ± 0.166
0.762GlnTyr: 0.762 ± 0.192
0.0GlnXaa: 0.0 ± 0.0
Arg
6.723ArgAla: 6.723 ± 0.509
0.554ArgCys: 0.554 ± 0.171
4.748ArgAsp: 4.748 ± 0.439
5.51ArgGlu: 5.51 ± 0.466
2.669ArgPhe: 2.669 ± 0.327
4.817ArgGly: 4.817 ± 0.366
1.698ArgHis: 1.698 ± 0.275
2.98ArgIle: 2.98 ± 0.329
3.674ArgLys: 3.674 ± 0.391
5.372ArgLeu: 5.372 ± 0.421
2.01ArgMet: 2.01 ± 0.213
2.669ArgAsn: 2.669 ± 0.291
3.154ArgPro: 3.154 ± 0.396
3.154ArgGln: 3.154 ± 0.41
5.614ArgArg: 5.614 ± 0.494
3.847ArgSer: 3.847 ± 0.51
3.881ArgThr: 3.881 ± 0.375
4.99ArgVal: 4.99 ± 0.434
1.629ArgTrp: 1.629 ± 0.253
2.01ArgTyr: 2.01 ± 0.254
0.0ArgXaa: 0.0 ± 0.0
Ser
5.164SerAla: 5.164 ± 0.472
0.485SerCys: 0.485 ± 0.146
4.02SerAsp: 4.02 ± 0.332
3.188SerGlu: 3.188 ± 0.377
1.248SerPhe: 1.248 ± 0.229
4.99SerGly: 4.99 ± 0.533
0.693SerHis: 0.693 ± 0.177
3.015SerIle: 3.015 ± 0.311
2.045SerLys: 2.045 ± 0.213
4.54SerLeu: 4.54 ± 0.468
1.109SerMet: 1.109 ± 0.224
1.594SerAsn: 1.594 ± 0.262
3.396SerPro: 3.396 ± 0.3
1.594SerGln: 1.594 ± 0.287
4.02SerArg: 4.02 ± 0.391
3.188SerSer: 3.188 ± 0.348
3.743SerThr: 3.743 ± 0.425
4.055SerVal: 4.055 ± 0.365
1.04SerTrp: 1.04 ± 0.177
1.767SerTyr: 1.767 ± 0.221
0.0SerXaa: 0.0 ± 0.0
Thr
6.723ThrAla: 6.723 ± 0.629
0.347ThrCys: 0.347 ± 0.129
3.951ThrAsp: 3.951 ± 0.333
3.362ThrGlu: 3.362 ± 0.353
1.733ThrPhe: 1.733 ± 0.238
5.961ThrGly: 5.961 ± 0.475
1.421ThrHis: 1.421 ± 0.256
3.847ThrIle: 3.847 ± 0.415
2.218ThrLys: 2.218 ± 0.235
4.609ThrLeu: 4.609 ± 0.47
1.282ThrMet: 1.282 ± 0.219
1.629ThrAsn: 1.629 ± 0.273
3.847ThrPro: 3.847 ± 0.399
2.01ThrGln: 2.01 ± 0.337
3.57ThrArg: 3.57 ± 0.364
2.703ThrSer: 2.703 ± 0.319
4.159ThrThr: 4.159 ± 0.362
3.708ThrVal: 3.708 ± 0.379
1.317ThrTrp: 1.317 ± 0.232
1.386ThrTyr: 1.386 ± 0.219
0.0ThrXaa: 0.0 ± 0.0
Val
7.798ValAla: 7.798 ± 0.518
0.589ValCys: 0.589 ± 0.156
5.58ValAsp: 5.58 ± 0.471
5.995ValGlu: 5.995 ± 0.537
1.837ValPhe: 1.837 ± 0.287
4.852ValGly: 4.852 ± 0.362
1.352ValHis: 1.352 ± 0.218
4.055ValIle: 4.055 ± 0.476
2.114ValLys: 2.114 ± 0.27
5.198ValLeu: 5.198 ± 0.471
1.352ValMet: 1.352 ± 0.226
2.218ValAsn: 2.218 ± 0.267
2.807ValPro: 2.807 ± 0.351
1.248ValGln: 1.248 ± 0.24
4.783ValArg: 4.783 ± 0.421
3.985ValSer: 3.985 ± 0.361
4.297ValThr: 4.297 ± 0.39
5.233ValVal: 5.233 ± 0.483
1.213ValTrp: 1.213 ± 0.243
1.733ValTyr: 1.733 ± 0.263
0.0ValXaa: 0.0 ± 0.0
Trp
1.629TrpAla: 1.629 ± 0.235
0.277TrpCys: 0.277 ± 0.102
1.906TrpAsp: 1.906 ± 0.291
1.144TrpGlu: 1.144 ± 0.21
0.451TrpPhe: 0.451 ± 0.111
1.317TrpGly: 1.317 ± 0.239
0.589TrpHis: 0.589 ± 0.161
1.178TrpIle: 1.178 ± 0.209
0.589TrpLys: 0.589 ± 0.151
1.525TrpLeu: 1.525 ± 0.264
0.797TrpMet: 0.797 ± 0.2
0.693TrpAsn: 0.693 ± 0.148
0.693TrpPro: 0.693 ± 0.179
0.589TrpGln: 0.589 ± 0.125
1.767TrpArg: 1.767 ± 0.309
1.386TrpSer: 1.386 ± 0.219
1.421TrpThr: 1.421 ± 0.193
1.248TrpVal: 1.248 ± 0.168
0.312TrpTrp: 0.312 ± 0.11
0.347TrpTyr: 0.347 ± 0.095
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.946TyrAla: 2.946 ± 0.306
0.208TyrCys: 0.208 ± 0.084
2.149TyrAsp: 2.149 ± 0.358
1.837TyrGlu: 1.837 ± 0.342
0.762TyrPhe: 0.762 ± 0.159
2.461TyrGly: 2.461 ± 0.305
0.554TyrHis: 0.554 ± 0.12
0.936TyrIle: 0.936 ± 0.187
0.901TyrLys: 0.901 ± 0.151
1.941TyrLeu: 1.941 ± 0.282
0.728TyrMet: 0.728 ± 0.17
1.005TyrAsn: 1.005 ± 0.204
1.282TyrPro: 1.282 ± 0.245
0.901TyrGln: 0.901 ± 0.151
2.357TyrArg: 2.357 ± 0.266
1.56TyrSer: 1.56 ± 0.259
1.109TyrThr: 1.109 ± 0.215
2.357TyrVal: 2.357 ± 0.348
0.52TyrTrp: 0.52 ± 0.13
0.728TyrTyr: 0.728 ± 0.167
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 168 proteins (28856 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski