Amino acid dipepetide frequency for Malonomonas rubra DSM 5091

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.336AlaAla: 9.336 ± 0.129	1.084AlaCys: 1.084 ± 0.039	5.333AlaAsp: 5.333 ± 0.079	7.341AlaGlu: 7.341 ± 0.093	3.287AlaPhe: 3.287 ± 0.062	7.254AlaGly: 7.254 ± 0.097	1.509AlaHis: 1.509 ± 0.039	5.337AlaIle: 5.337 ± 0.069	4.393AlaLys: 4.393 ± 0.065	9.391AlaLeu: 9.391 ± 0.104	2.324AlaMet: 2.324 ± 0.048	2.677AlaAsn: 2.677 ± 0.052	2.924AlaPro: 2.924 ± 0.059	3.171AlaGln: 3.171 ± 0.058	4.227AlaArg: 4.227 ± 0.064	4.614AlaSer: 4.614 ± 0.086	4.482AlaThr: 4.482 ± 0.074	6.334AlaVal: 6.334 ± 0.089	0.78AlaTrp: 0.78 ± 0.028	2.135AlaTyr: 2.135 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.937CysAla: 0.937 ± 0.028	0.235CysCys: 0.235 ± 0.014	0.662CysAsp: 0.662 ± 0.026	0.699CysGlu: 0.699 ± 0.024	0.519CysPhe: 0.519 ± 0.021	1.235CysGly: 1.235 ± 0.034	0.795CysHis: 0.795 ± 0.083	0.545CysIle: 0.545 ± 0.023	0.475CysLys: 0.475 ± 0.017	1.243CysLeu: 1.243 ± 0.036	0.261CysMet: 0.261 ± 0.015	0.429CysAsn: 0.429 ± 0.02	0.685CysPro: 0.685 ± 0.029	0.478CysGln: 0.478 ± 0.023	0.814CysArg: 0.814 ± 0.029	0.895CysSer: 0.895 ± 0.033	0.54CysThr: 0.54 ± 0.023	0.683CysVal: 0.683 ± 0.026	0.146CysTrp: 0.146 ± 0.012	0.353CysTyr: 0.353 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.482AspAla: 4.482 ± 0.075	0.847AspCys: 0.847 ± 0.036	3.124AspAsp: 3.124 ± 0.066	3.99AspGlu: 3.99 ± 0.059	2.68AspPhe: 2.68 ± 0.055	4.204AspGly: 4.204 ± 0.117	1.066AspHis: 1.066 ± 0.03	3.445AspIle: 3.445 ± 0.058	2.644AspLys: 2.644 ± 0.054	6.324AspLeu: 6.324 ± 0.08	1.347AspMet: 1.347 ± 0.033	1.977AspAsn: 1.977 ± 0.039	2.666AspPro: 2.666 ± 0.059	2.281AspGln: 2.281 ± 0.052	3.056AspArg: 3.056 ± 0.049	3.292AspSer: 3.292 ± 0.076	2.173AspThr: 2.173 ± 0.051	3.593AspVal: 3.593 ± 0.061	0.769AspTrp: 0.769 ± 0.027	2.008AspTyr: 2.008 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	5.691GluAla: 5.691 ± 0.083	0.621GluCys: 0.621 ± 0.026	3.308GluAsp: 3.308 ± 0.066	5.486GluGlu: 5.486 ± 0.097	2.26GluPhe: 2.26 ± 0.044	4.189GluGly: 4.189 ± 0.054	1.31GluHis: 1.31 ± 0.034	4.617GluIle: 4.617 ± 0.076	4.989GluLys: 4.989 ± 0.078	8.016GluLeu: 8.016 ± 0.11	2.038GluMet: 2.038 ± 0.043	2.67GluAsn: 2.67 ± 0.047	2.3GluPro: 2.3 ± 0.052	4.143GluGln: 4.143 ± 0.074	4.007GluArg: 4.007 ± 0.061	3.37GluSer: 3.37 ± 0.051	3.452GluThr: 3.452 ± 0.064	4.364GluVal: 4.364 ± 0.061	0.57GluTrp: 0.57 ± 0.023	1.686GluTyr: 1.686 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	3.674PheAla: 3.674 ± 0.058	0.611PheCys: 0.611 ± 0.025	2.626PheAsp: 2.626 ± 0.049	2.296PheGlu: 2.296 ± 0.04	2.03PhePhe: 2.03 ± 0.055	3.373PheGly: 3.373 ± 0.065	0.825PheHis: 0.825 ± 0.026	2.302PheIle: 2.302 ± 0.046	1.697PheLys: 1.697 ± 0.043	4.211PheLeu: 4.211 ± 0.075	0.882PheMet: 0.882 ± 0.03	1.541PheAsn: 1.541 ± 0.034	1.697PhePro: 1.697 ± 0.042	1.302PheGln: 1.302 ± 0.035	2.074PheArg: 2.074 ± 0.046	3.298PheSer: 3.298 ± 0.06	2.051PheThr: 2.051 ± 0.046	2.766PheVal: 2.766 ± 0.05	0.547PheTrp: 0.547 ± 0.022	1.253PheTyr: 1.253 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	5.642GlyAla: 5.642 ± 0.079	1.181GlyCys: 1.181 ± 0.037	4.051GlyAsp: 4.051 ± 0.079	4.882GlyGlu: 4.882 ± 0.065	3.184GlyPhe: 3.184 ± 0.062	5.644GlyGly: 5.644 ± 0.095	1.608GlyHis: 1.608 ± 0.037	4.991GlyIle: 4.991 ± 0.075	4.436GlyLys: 4.436 ± 0.072	7.8GlyLeu: 7.8 ± 0.104	2.279GlyMet: 2.279 ± 0.043	2.499GlyAsn: 2.499 ± 0.055	2.148GlyPro: 2.148 ± 0.045	2.812GlyGln: 2.812 ± 0.057	4.045GlyArg: 4.045 ± 0.06	4.406GlySer: 4.406 ± 0.096	3.775GlyThr: 3.775 ± 0.074	5.379GlyVal: 5.379 ± 0.077	0.936GlyTrp: 0.936 ± 0.029	2.571GlyTyr: 2.571 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	1.542HisAla: 1.542 ± 0.046	0.31HisCys: 0.31 ± 0.016	1.076HisAsp: 1.076 ± 0.037	1.139HisGlu: 1.139 ± 0.03	0.948HisPhe: 0.948 ± 0.029	1.582HisGly: 1.582 ± 0.037	0.576HisHis: 0.576 ± 0.03	1.05HisIle: 1.05 ± 0.032	0.897HisLys: 0.897 ± 0.027	2.245HisLeu: 2.245 ± 0.045	0.415HisMet: 0.415 ± 0.019	0.749HisAsn: 0.749 ± 0.024	1.245HisPro: 1.245 ± 0.042	0.985HisGln: 0.985 ± 0.03	1.167HisArg: 1.167 ± 0.037	1.252HisSer: 1.252 ± 0.036	0.811HisThr: 0.811 ± 0.027	1.109HisVal: 1.109 ± 0.03	0.274HisTrp: 0.274 ± 0.015	0.662HisTyr: 0.662 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.617IleAla: 5.617 ± 0.079	0.897IleCys: 0.897 ± 0.029	4.411IleAsp: 4.411 ± 0.066	4.421IleGlu: 4.421 ± 0.07	2.495IlePhe: 2.495 ± 0.046	4.795IleGly: 4.795 ± 0.074	1.109IleHis: 1.109 ± 0.027	3.527IleIle: 3.527 ± 0.067	2.866IleLys: 2.866 ± 0.049	5.78IleLeu: 5.78 ± 0.077	1.209IleMet: 1.209 ± 0.037	2.349IleAsn: 2.349 ± 0.05	2.747IlePro: 2.747 ± 0.055	1.666IleGln: 1.666 ± 0.04	3.033IleArg: 3.033 ± 0.055	4.267IleSer: 4.267 ± 0.063	2.755IleThr: 2.755 ± 0.052	3.893IleVal: 3.893 ± 0.063	0.528IleTrp: 0.528 ± 0.022	1.675IleTyr: 1.675 ± 0.04	0.0IleXaa: 0.0 ± 0.0
Lys	4.323LysAla: 4.323 ± 0.066	0.442LysCys: 0.442 ± 0.023	2.585LysAsp: 2.585 ± 0.053	3.784LysGlu: 3.784 ± 0.062	1.542LysPhe: 1.542 ± 0.038	3.367LysGly: 3.367 ± 0.062	0.98LysHis: 0.98 ± 0.032	3.558LysIle: 3.558 ± 0.057	3.654LysLys: 3.654 ± 0.075	5.396LysLeu: 5.396 ± 0.08	1.469LysMet: 1.469 ± 0.031	2.041LysAsn: 2.041 ± 0.042	2.256LysPro: 2.256 ± 0.052	2.693LysGln: 2.693 ± 0.058	2.82LysArg: 2.82 ± 0.054	2.704LysSer: 2.704 ± 0.052	2.774LysThr: 2.774 ± 0.049	3.744LysVal: 3.744 ± 0.061	0.42LysTrp: 0.42 ± 0.024	1.295LysTyr: 1.295 ± 0.038	0.0LysXaa: 0.0 ± 0.0
Leu	10.402LeuAla: 10.402 ± 0.12	1.323LeuCys: 1.323 ± 0.035	5.97LeuAsp: 5.97 ± 0.072	7.063LeuGlu: 7.063 ± 0.1	4.656LeuPhe: 4.656 ± 0.085	7.699LeuGly: 7.699 ± 0.094	2.092LeuHis: 2.092 ± 0.044	6.171LeuIle: 6.171 ± 0.094	5.665LeuLys: 5.665 ± 0.078	13.889LeuLeu: 13.889 ± 0.195	2.339LeuMet: 2.339 ± 0.047	3.72LeuAsn: 3.72 ± 0.061	5.487LeuPro: 5.487 ± 0.082	5.583LeuGln: 5.583 ± 0.095	6.083LeuArg: 6.083 ± 0.078	7.08LeuSer: 7.08 ± 0.082	5.463LeuThr: 5.463 ± 0.073	7.384LeuVal: 7.384 ± 0.097	0.962LeuTrp: 0.962 ± 0.031	2.518LeuTyr: 2.518 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	2.493MetAla: 2.493 ± 0.051	0.171MetCys: 0.171 ± 0.011	1.149MetAsp: 1.149 ± 0.035	1.485MetGlu: 1.485 ± 0.037	0.825MetPhe: 0.825 ± 0.029	1.629MetGly: 1.629 ± 0.045	0.404MetHis: 0.404 ± 0.017	1.507MetIle: 1.507 ± 0.039	1.51MetLys: 1.51 ± 0.037	2.817MetLeu: 2.817 ± 0.053	0.667MetMet: 0.667 ± 0.024	0.922MetAsn: 0.922 ± 0.029	1.199MetPro: 1.199 ± 0.031	1.112MetGln: 1.112 ± 0.028	1.338MetArg: 1.338 ± 0.035	1.609MetSer: 1.609 ± 0.038	1.491MetThr: 1.491 ± 0.036	1.711MetVal: 1.711 ± 0.04	0.123MetTrp: 0.123 ± 0.01	0.435MetTyr: 0.435 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.75AsnAla: 2.75 ± 0.055	0.502AsnCys: 0.502 ± 0.023	1.836AsnAsp: 1.836 ± 0.052	1.965AsnGlu: 1.965 ± 0.04	1.452AsnPhe: 1.452 ± 0.036	2.553AsnGly: 2.553 ± 0.061	0.729AsnHis: 0.729 ± 0.025	2.198AsnIle: 2.198 ± 0.044	1.661AsnLys: 1.661 ± 0.036	4.09AsnLeu: 4.09 ± 0.052	0.834AsnMet: 0.834 ± 0.027	1.328AsnAsn: 1.328 ± 0.03	2.035AsnPro: 2.035 ± 0.043	1.455AsnGln: 1.455 ± 0.034	2.146AsnArg: 2.146 ± 0.044	2.09AsnSer: 2.09 ± 0.055	1.421AsnThr: 1.421 ± 0.039	2.132AsnVal: 2.132 ± 0.049	0.449AsnTrp: 0.449 ± 0.019	1.22AsnTyr: 1.22 ± 0.039	0.0AsnXaa: 0.0 ± 0.0
Pro	3.783ProAla: 3.783 ± 0.064	0.411ProCys: 0.411 ± 0.017	2.633ProAsp: 2.633 ± 0.047	3.914ProGlu: 3.914 ± 0.064	1.884ProPhe: 1.884 ± 0.042	3.224ProGly: 3.224 ± 0.054	0.875ProHis: 0.875 ± 0.031	2.265ProIle: 2.265 ± 0.037	1.997ProLys: 1.997 ± 0.045	4.714ProLeu: 4.714 ± 0.065	0.902ProMet: 0.902 ± 0.026	1.24ProAsn: 1.24 ± 0.035	1.603ProPro: 1.603 ± 0.039	2.143ProGln: 2.143 ± 0.05	1.688ProArg: 1.688 ± 0.036	2.198ProSer: 2.198 ± 0.044	1.975ProThr: 1.975 ± 0.042	3.444ProVal: 3.444 ± 0.056	0.472ProTrp: 0.472 ± 0.024	1.174ProTyr: 1.174 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	4.003GlnAla: 4.003 ± 0.072	0.35GlnCys: 0.35 ± 0.018	1.865GlnAsp: 1.865 ± 0.044	2.755GlnGlu: 2.755 ± 0.059	1.411GlnPhe: 1.411 ± 0.036	2.771GlnGly: 2.771 ± 0.05	0.944GlnHis: 0.944 ± 0.029	2.777GlnIle: 2.777 ± 0.054	2.453GlnLys: 2.453 ± 0.054	5.548GlnLeu: 5.548 ± 0.082	1.218GlnMet: 1.218 ± 0.032	1.48GlnAsn: 1.48 ± 0.037	1.912GlnPro: 1.912 ± 0.049	3.436GlnGln: 3.436 ± 0.092	2.738GlnArg: 2.738 ± 0.059	2.133GlnSer: 2.133 ± 0.054	1.98GlnThr: 1.98 ± 0.039	3.403GlnVal: 3.403 ± 0.06	0.42GlnTrp: 0.42 ± 0.022	0.907GlnTyr: 0.907 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	3.81ArgAla: 3.81 ± 0.068	0.682ArgCys: 0.682 ± 0.027	2.841ArgAsp: 2.841 ± 0.051	3.992ArgGlu: 3.992 ± 0.059	2.513ArgPhe: 2.513 ± 0.053	3.296ArgGly: 3.296 ± 0.058	1.124ArgHis: 1.124 ± 0.036	3.49ArgIle: 3.49 ± 0.055	3.189ArgLys: 3.189 ± 0.055	6.169ArgLeu: 6.169 ± 0.094	1.467ArgMet: 1.467 ± 0.041	2.053ArgAsn: 2.053 ± 0.041	2.002ArgPro: 2.002 ± 0.042	2.944ArgGln: 2.944 ± 0.059	3.259ArgArg: 3.259 ± 0.069	3.136ArgSer: 3.136 ± 0.053	2.35ArgThr: 2.35 ± 0.047	3.52ArgVal: 3.52 ± 0.063	0.695ArgTrp: 0.695 ± 0.026	1.8ArgTyr: 1.8 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	5.274SerAla: 5.274 ± 0.082	0.907SerCys: 0.907 ± 0.046	3.28SerAsp: 3.28 ± 0.063	3.679SerGlu: 3.679 ± 0.064	2.844SerPhe: 2.844 ± 0.055	5.273SerGly: 5.273 ± 0.079	1.18SerHis: 1.18 ± 0.032	3.442SerIle: 3.442 ± 0.062	2.636SerLys: 2.636 ± 0.051	6.808SerLeu: 6.808 ± 0.086	1.46SerMet: 1.46 ± 0.034	1.931SerAsn: 1.931 ± 0.047	2.519SerPro: 2.519 ± 0.048	2.33SerGln: 2.33 ± 0.048	3.219SerArg: 3.219 ± 0.062	3.961SerSer: 3.961 ± 0.082	2.811SerThr: 2.811 ± 0.058	3.91SerVal: 3.91 ± 0.081	0.746SerTrp: 0.746 ± 0.027	1.779SerTyr: 1.779 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	4.484ThrAla: 4.484 ± 0.075	0.646ThrCys: 0.646 ± 0.023	2.831ThrAsp: 2.831 ± 0.062	3.215ThrGlu: 3.215 ± 0.051	2.017ThrPhe: 2.017 ± 0.051	4.362ThrGly: 4.362 ± 0.075	0.883ThrHis: 0.883 ± 0.026	2.928ThrIle: 2.928 ± 0.056	1.8ThrLys: 1.8 ± 0.041	5.447ThrLeu: 5.447 ± 0.072	1.058ThrMet: 1.058 ± 0.031	1.542ThrAsn: 1.542 ± 0.043	2.442ThrPro: 2.442 ± 0.053	1.45ThrGln: 1.45 ± 0.037	2.284ThrArg: 2.284 ± 0.036	2.904ThrSer: 2.904 ± 0.068	2.584ThrThr: 2.584 ± 0.056	3.598ThrVal: 3.598 ± 0.069	0.404ThrTrp: 0.404 ± 0.019	1.296ThrTyr: 1.296 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	6.762ValAla: 6.762 ± 0.089	0.88ValCys: 0.88 ± 0.026	4.318ValAsp: 4.318 ± 0.072	5.03ValGlu: 5.03 ± 0.082	2.69ValPhe: 2.69 ± 0.055	4.938ValGly: 4.938 ± 0.071	1.151ValHis: 1.151 ± 0.033	4.051ValIle: 4.051 ± 0.061	3.325ValLys: 3.325 ± 0.053	7.017ValLeu: 7.017 ± 0.08	1.688ValMet: 1.688 ± 0.043	2.327ValAsn: 2.327 ± 0.055	2.946ValPro: 2.946 ± 0.053	2.277ValGln: 2.277 ± 0.043	3.554ValArg: 3.554 ± 0.064	4.313ValSer: 4.313 ± 0.07	3.631ValThr: 3.631 ± 0.071	5.367ValVal: 5.367 ± 0.076	0.63ValTrp: 0.63 ± 0.024	1.651ValTyr: 1.651 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.77TrpAla: 0.77 ± 0.027	0.138TrpCys: 0.138 ± 0.011	0.503TrpAsp: 0.503 ± 0.022	0.54TrpGlu: 0.54 ± 0.024	0.447TrpPhe: 0.447 ± 0.021	0.78TrpGly: 0.78 ± 0.029	0.238TrpHis: 0.238 ± 0.013	0.543TrpIle: 0.543 ± 0.021	0.48TrpLys: 0.48 ± 0.021	1.334TrpLeu: 1.334 ± 0.038	0.253TrpMet: 0.253 ± 0.014	0.404TrpAsn: 0.404 ± 0.02	0.408TrpPro: 0.408 ± 0.019	0.739TrpGln: 0.739 ± 0.027	0.671TrpArg: 0.671 ± 0.027	0.588TrpSer: 0.588 ± 0.024	0.439TrpThr: 0.439 ± 0.02	0.643TrpVal: 0.643 ± 0.023	0.159TrpTrp: 0.159 ± 0.013	0.329TrpTyr: 0.329 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.157TyrAla: 2.157 ± 0.05	0.392TyrCys: 0.392 ± 0.019	1.566TyrAsp: 1.566 ± 0.044	1.409TyrGlu: 1.409 ± 0.034	1.372TyrPhe: 1.372 ± 0.036	2.102TyrGly: 2.102 ± 0.045	0.634TyrHis: 0.634 ± 0.026	1.295TyrIle: 1.295 ± 0.036	1.081TyrLys: 1.081 ± 0.032	3.311TyrLeu: 3.311 ± 0.072	0.488TyrMet: 0.488 ± 0.022	0.971TyrAsn: 0.971 ± 0.027	1.317TyrPro: 1.317 ± 0.036	1.501TyrGln: 1.501 ± 0.034	2.108TyrArg: 2.108 ± 0.046	1.883TyrSer: 1.883 ± 0.049	1.217TyrThr: 1.217 ± 0.043	1.567TyrVal: 1.567 ± 0.041	0.375TyrTrp: 0.375 ± 0.017	0.909TyrTyr: 0.909 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3654 proteins (1195547 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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