Amino acid dipepetide frequency for Proteus phage PM 85

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.128AlaAla: 3.128 ± 0.926
0.552AlaCys: 0.552 ± 0.256
4.875AlaAsp: 4.875 ± 0.743
5.151AlaGlu: 5.151 ± 0.61
3.128AlaPhe: 3.128 ± 0.547
5.887AlaGly: 5.887 ± 1.124
1.564AlaHis: 1.564 ± 0.348
5.427AlaIle: 5.427 ± 0.7
5.979AlaLys: 5.979 ± 0.897
6.071AlaLeu: 6.071 ± 0.614
3.772AlaMet: 3.772 ± 0.807
3.312AlaAsn: 3.312 ± 0.474
2.484AlaPro: 2.484 ± 0.622
4.691AlaGln: 4.691 ± 0.89
4.599AlaArg: 4.599 ± 0.692
6.163AlaSer: 6.163 ± 0.813
3.68AlaThr: 3.68 ± 0.612
4.599AlaVal: 4.599 ± 0.632
0.736AlaTrp: 0.736 ± 0.213
2.76AlaTyr: 2.76 ± 0.469
0.0AlaXaa: 0.0 ± 0.0
Cys
0.644CysAla: 0.644 ± 0.204
0.184CysCys: 0.184 ± 0.148
0.46CysAsp: 0.46 ± 0.231
0.46CysGlu: 0.46 ± 0.175
0.092CysPhe: 0.092 ± 0.084
0.552CysGly: 0.552 ± 0.243
0.092CysHis: 0.092 ± 0.098
0.368CysIle: 0.368 ± 0.216
0.736CysLys: 0.736 ± 0.348
1.196CysLeu: 1.196 ± 0.27
0.552CysMet: 0.552 ± 0.229
0.276CysAsn: 0.276 ± 0.197
0.46CysPro: 0.46 ± 0.18
0.092CysGln: 0.092 ± 0.079
0.552CysArg: 0.552 ± 0.245
0.644CysSer: 0.644 ± 0.292
0.276CysThr: 0.276 ± 0.148
1.012CysVal: 1.012 ± 0.4
0.184CysTrp: 0.184 ± 0.124
0.368CysTyr: 0.368 ± 0.223
0.0CysXaa: 0.0 ± 0.0
Asp
5.611AspAla: 5.611 ± 0.613
0.46AspCys: 0.46 ± 0.205
3.404AspAsp: 3.404 ± 0.602
5.703AspGlu: 5.703 ± 0.799
2.116AspPhe: 2.116 ± 0.384
4.967AspGly: 4.967 ± 0.755
1.104AspHis: 1.104 ± 0.248
4.139AspIle: 4.139 ± 0.555
4.967AspLys: 4.967 ± 0.616
4.783AspLeu: 4.783 ± 0.645
2.3AspMet: 2.3 ± 0.343
3.772AspAsn: 3.772 ± 0.675
1.748AspPro: 1.748 ± 0.354
0.644AspGln: 0.644 ± 0.272
2.392AspArg: 2.392 ± 0.266
3.955AspSer: 3.955 ± 0.511
4.231AspThr: 4.231 ± 0.484
4.967AspVal: 4.967 ± 0.688
1.012AspTrp: 1.012 ± 0.388
2.024AspTyr: 2.024 ± 0.335
0.0AspXaa: 0.0 ± 0.0
Glu
5.979GluAla: 5.979 ± 0.854
1.104GluCys: 1.104 ± 0.443
4.967GluAsp: 4.967 ± 0.545
5.795GluGlu: 5.795 ± 0.97
2.576GluPhe: 2.576 ± 0.424
4.783GluGly: 4.783 ± 0.704
1.564GluHis: 1.564 ± 0.307
3.955GluIle: 3.955 ± 0.466
4.323GluLys: 4.323 ± 0.631
6.071GluLeu: 6.071 ± 1.002
2.392GluMet: 2.392 ± 0.477
2.944GluAsn: 2.944 ± 0.589
1.932GluPro: 1.932 ± 0.408
3.955GluGln: 3.955 ± 0.588
3.68GluArg: 3.68 ± 0.651
2.3GluSer: 2.3 ± 0.398
1.656GluThr: 1.656 ± 0.311
6.807GluVal: 6.807 ± 0.804
1.472GluTrp: 1.472 ± 0.283
1.84GluTyr: 1.84 ± 0.439
0.0GluXaa: 0.0 ± 0.0
Phe
2.116PheAla: 2.116 ± 0.385
0.46PheCys: 0.46 ± 0.197
3.588PheAsp: 3.588 ± 0.654
2.668PheGlu: 2.668 ± 0.285
1.564PhePhe: 1.564 ± 0.404
3.036PheGly: 3.036 ± 0.633
0.552PheHis: 0.552 ± 0.2
3.128PheIle: 3.128 ± 0.349
2.944PheLys: 2.944 ± 0.503
3.772PheLeu: 3.772 ± 0.475
1.564PheMet: 1.564 ± 0.249
2.668PheAsn: 2.668 ± 0.498
1.104PhePro: 1.104 ± 0.284
0.92PheGln: 0.92 ± 0.337
1.84PheArg: 1.84 ± 0.344
2.208PheSer: 2.208 ± 0.431
2.208PheThr: 2.208 ± 0.556
1.656PheVal: 1.656 ± 0.391
0.092PheTrp: 0.092 ± 0.086
1.012PheTyr: 1.012 ± 0.309
0.0PheXaa: 0.0 ± 0.0
Gly
5.979GlyAla: 5.979 ± 0.653
0.736GlyCys: 0.736 ± 0.312
6.899GlyAsp: 6.899 ± 0.81
4.691GlyGlu: 4.691 ± 0.706
3.772GlyPhe: 3.772 ± 0.621
5.519GlyGly: 5.519 ± 0.889
1.748GlyHis: 1.748 ± 0.265
4.507GlyIle: 4.507 ± 0.462
6.991GlyLys: 6.991 ± 0.987
5.243GlyLeu: 5.243 ± 0.825
1.472GlyMet: 1.472 ± 0.339
3.22GlyAsn: 3.22 ± 0.469
0.0GlyPro: 0.0 ± 0.0
2.76GlyGln: 2.76 ± 0.387
3.588GlyArg: 3.588 ± 0.451
5.151GlySer: 5.151 ± 0.635
5.795GlyThr: 5.795 ± 0.666
5.059GlyVal: 5.059 ± 0.842
1.564GlyTrp: 1.564 ± 0.355
3.22GlyTyr: 3.22 ± 0.407
0.0GlyXaa: 0.0 ± 0.0
His
1.196HisAla: 1.196 ± 0.406
0.092HisCys: 0.092 ± 0.079
1.288HisAsp: 1.288 ± 0.311
1.104HisGlu: 1.104 ± 0.391
1.012HisPhe: 1.012 ± 0.198
1.932HisGly: 1.932 ± 0.533
0.276HisHis: 0.276 ± 0.173
1.012HisIle: 1.012 ± 0.241
1.288HisLys: 1.288 ± 0.319
2.392HisLeu: 2.392 ± 0.55
0.828HisMet: 0.828 ± 0.394
1.196HisAsn: 1.196 ± 0.419
0.276HisPro: 0.276 ± 0.141
0.644HisGln: 0.644 ± 0.188
1.012HisArg: 1.012 ± 0.298
0.828HisSer: 0.828 ± 0.287
1.104HisThr: 1.104 ± 0.274
0.828HisVal: 0.828 ± 0.314
0.184HisTrp: 0.184 ± 0.116
0.552HisTyr: 0.552 ± 0.219
0.0HisXaa: 0.0 ± 0.0
Ile
4.323IleAla: 4.323 ± 0.529
0.46IleCys: 0.46 ± 0.165
4.415IleAsp: 4.415 ± 0.574
3.772IleGlu: 3.772 ± 0.462
1.748IlePhe: 1.748 ± 0.452
4.875IleGly: 4.875 ± 0.538
1.196IleHis: 1.196 ± 0.283
4.139IleIle: 4.139 ± 0.919
4.507IleLys: 4.507 ± 0.571
3.496IleLeu: 3.496 ± 0.691
2.3IleMet: 2.3 ± 0.571
3.404IleAsn: 3.404 ± 0.519
2.76IlePro: 2.76 ± 0.471
1.932IleGln: 1.932 ± 0.373
2.944IleArg: 2.944 ± 0.578
3.036IleSer: 3.036 ± 0.532
3.312IleThr: 3.312 ± 0.721
3.496IleVal: 3.496 ± 0.655
0.828IleTrp: 0.828 ± 0.248
1.564IleTyr: 1.564 ± 0.529
0.0IleXaa: 0.0 ± 0.0
Lys
6.347LysAla: 6.347 ± 0.785
0.368LysCys: 0.368 ± 0.144
4.139LysAsp: 4.139 ± 0.662
5.335LysGlu: 5.335 ± 0.691
1.564LysPhe: 1.564 ± 0.413
6.255LysGly: 6.255 ± 0.805
1.288LysHis: 1.288 ± 0.461
3.312LysIle: 3.312 ± 0.42
3.22LysLys: 3.22 ± 0.481
5.519LysLeu: 5.519 ± 0.773
1.196LysMet: 1.196 ± 0.339
2.576LysAsn: 2.576 ± 0.493
3.404LysPro: 3.404 ± 0.448
2.852LysGln: 2.852 ± 0.462
3.772LysArg: 3.772 ± 0.537
4.415LysSer: 4.415 ± 0.469
2.76LysThr: 2.76 ± 0.444
5.427LysVal: 5.427 ± 0.885
0.828LysTrp: 0.828 ± 0.237
2.576LysTyr: 2.576 ± 0.347
0.0LysXaa: 0.0 ± 0.0
Leu
5.887LeuAla: 5.887 ± 1.124
0.552LeuCys: 0.552 ± 0.24
5.151LeuAsp: 5.151 ± 0.631
5.611LeuGlu: 5.611 ± 0.772
2.852LeuPhe: 2.852 ± 0.664
5.427LeuGly: 5.427 ± 0.65
0.92LeuHis: 0.92 ± 0.375
4.231LeuIle: 4.231 ± 0.549
5.795LeuLys: 5.795 ± 0.841
5.519LeuLeu: 5.519 ± 0.549
1.564LeuMet: 1.564 ± 0.326
3.68LeuAsn: 3.68 ± 0.477
4.323LeuPro: 4.323 ± 0.595
4.691LeuGln: 4.691 ± 1.012
4.415LeuArg: 4.415 ± 0.761
4.691LeuSer: 4.691 ± 0.75
4.231LeuThr: 4.231 ± 0.471
4.507LeuVal: 4.507 ± 0.733
0.828LeuTrp: 0.828 ± 0.225
2.576LeuTyr: 2.576 ± 0.4
0.0LeuXaa: 0.0 ± 0.0
Met
3.496MetAla: 3.496 ± 0.434
0.276MetCys: 0.276 ± 0.175
1.104MetAsp: 1.104 ± 0.409
2.208MetGlu: 2.208 ± 0.414
1.196MetPhe: 1.196 ± 0.274
2.3MetGly: 2.3 ± 0.472
0.46MetHis: 0.46 ± 0.136
1.38MetIle: 1.38 ± 0.365
2.024MetLys: 2.024 ± 0.42
2.852MetLeu: 2.852 ± 0.464
0.644MetMet: 0.644 ± 0.238
1.196MetAsn: 1.196 ± 0.294
1.012MetPro: 1.012 ± 0.212
1.564MetGln: 1.564 ± 0.415
1.84MetArg: 1.84 ± 0.508
3.128MetSer: 3.128 ± 0.436
1.84MetThr: 1.84 ± 0.253
1.656MetVal: 1.656 ± 0.375
0.46MetTrp: 0.46 ± 0.276
1.012MetTyr: 1.012 ± 0.249
0.0MetXaa: 0.0 ± 0.0
Asn
3.128AsnAla: 3.128 ± 0.38
0.92AsnCys: 0.92 ± 0.314
1.932AsnAsp: 1.932 ± 0.384
2.484AsnGlu: 2.484 ± 0.659
1.748AsnPhe: 1.748 ± 0.437
3.955AsnGly: 3.955 ± 0.621
0.92AsnHis: 0.92 ± 0.326
2.76AsnIle: 2.76 ± 0.524
3.404AsnLys: 3.404 ± 0.616
3.772AsnLeu: 3.772 ± 0.445
1.288AsnMet: 1.288 ± 0.346
2.668AsnAsn: 2.668 ± 0.58
2.024AsnPro: 2.024 ± 0.348
2.484AsnGln: 2.484 ± 0.473
3.22AsnArg: 3.22 ± 0.461
2.024AsnSer: 2.024 ± 0.518
3.128AsnThr: 3.128 ± 0.603
3.496AsnVal: 3.496 ± 0.623
0.46AsnTrp: 0.46 ± 0.171
1.656AsnTyr: 1.656 ± 0.394
0.0AsnXaa: 0.0 ± 0.0
Pro
3.128ProAla: 3.128 ± 0.445
0.552ProCys: 0.552 ± 0.184
2.852ProAsp: 2.852 ± 0.511
3.312ProGlu: 3.312 ± 0.442
1.288ProPhe: 1.288 ± 0.323
0.276ProGly: 0.276 ± 0.131
0.828ProHis: 0.828 ± 0.25
1.196ProIle: 1.196 ± 0.309
1.932ProLys: 1.932 ± 0.426
1.84ProLeu: 1.84 ± 0.374
1.012ProMet: 1.012 ± 0.264
1.932ProAsn: 1.932 ± 0.549
0.828ProPro: 0.828 ± 0.244
1.196ProGln: 1.196 ± 0.256
1.288ProArg: 1.288 ± 0.282
2.208ProSer: 2.208 ± 0.474
2.392ProThr: 2.392 ± 0.38
2.576ProVal: 2.576 ± 0.583
0.736ProTrp: 0.736 ± 0.259
2.024ProTyr: 2.024 ± 0.407
0.0ProXaa: 0.0 ± 0.0
Gln
3.588GlnAla: 3.588 ± 0.712
0.276GlnCys: 0.276 ± 0.161
2.392GlnAsp: 2.392 ± 0.397
3.036GlnGlu: 3.036 ± 0.474
2.024GlnPhe: 2.024 ± 0.432
4.783GlnGly: 4.783 ± 0.597
0.828GlnHis: 0.828 ± 0.237
2.024GlnIle: 2.024 ± 0.322
1.472GlnLys: 1.472 ± 0.354
3.772GlnLeu: 3.772 ± 0.541
1.84GlnMet: 1.84 ± 0.406
1.196GlnAsn: 1.196 ± 0.241
1.196GlnPro: 1.196 ± 0.316
2.76GlnGln: 2.76 ± 0.617
2.668GlnArg: 2.668 ± 0.611
1.932GlnSer: 1.932 ± 0.431
2.484GlnThr: 2.484 ± 0.36
2.392GlnVal: 2.392 ± 0.373
0.92GlnTrp: 0.92 ± 0.325
1.38GlnTyr: 1.38 ± 0.363
0.0GlnXaa: 0.0 ± 0.0
Arg
5.519ArgAla: 5.519 ± 0.859
0.368ArgCys: 0.368 ± 0.228
3.312ArgAsp: 3.312 ± 0.51
3.68ArgGlu: 3.68 ± 0.497
2.024ArgPhe: 2.024 ± 0.264
4.323ArgGly: 4.323 ± 0.833
1.656ArgHis: 1.656 ± 0.358
3.496ArgIle: 3.496 ± 0.635
2.668ArgLys: 2.668 ± 0.474
3.955ArgLeu: 3.955 ± 0.73
2.3ArgMet: 2.3 ± 0.424
2.392ArgAsn: 2.392 ± 0.408
1.472ArgPro: 1.472 ± 0.344
1.38ArgGln: 1.38 ± 0.304
3.128ArgArg: 3.128 ± 0.516
3.036ArgSer: 3.036 ± 0.512
2.392ArgThr: 2.392 ± 0.505
3.863ArgVal: 3.863 ± 0.45
0.644ArgTrp: 0.644 ± 0.253
2.024ArgTyr: 2.024 ± 0.39
0.0ArgXaa: 0.0 ± 0.0
Ser
5.151SerAla: 5.151 ± 0.762
0.368SerCys: 0.368 ± 0.18
3.863SerAsp: 3.863 ± 0.519
3.588SerGlu: 3.588 ± 0.512
2.852SerPhe: 2.852 ± 0.46
5.151SerGly: 5.151 ± 0.697
0.828SerHis: 0.828 ± 0.27
4.323SerIle: 4.323 ± 0.513
3.496SerLys: 3.496 ± 0.469
4.875SerLeu: 4.875 ± 0.59
1.196SerMet: 1.196 ± 0.215
3.036SerAsn: 3.036 ± 0.446
1.748SerPro: 1.748 ± 0.402
2.852SerGln: 2.852 ± 0.54
3.588SerArg: 3.588 ± 0.507
2.944SerSer: 2.944 ± 0.776
3.312SerThr: 3.312 ± 0.548
4.231SerVal: 4.231 ± 0.477
1.104SerTrp: 1.104 ± 0.203
1.748SerTyr: 1.748 ± 0.416
0.0SerXaa: 0.0 ± 0.0
Thr
3.68ThrAla: 3.68 ± 0.531
0.552ThrCys: 0.552 ± 0.18
2.944ThrAsp: 2.944 ± 0.725
4.047ThrGlu: 4.047 ± 0.519
2.76ThrPhe: 2.76 ± 0.506
4.691ThrGly: 4.691 ± 0.704
1.104ThrHis: 1.104 ± 0.304
3.036ThrIle: 3.036 ± 0.475
3.955ThrLys: 3.955 ± 0.714
5.151ThrLeu: 5.151 ± 0.556
1.932ThrMet: 1.932 ± 0.341
2.024ThrAsn: 2.024 ± 0.456
1.932ThrPro: 1.932 ± 0.348
2.484ThrGln: 2.484 ± 0.461
2.76ThrArg: 2.76 ± 0.424
3.496ThrSer: 3.496 ± 0.703
3.036ThrThr: 3.036 ± 0.587
2.668ThrVal: 2.668 ± 0.69
0.46ThrTrp: 0.46 ± 0.209
1.564ThrTyr: 1.564 ± 0.384
0.0ThrXaa: 0.0 ± 0.0
Val
6.163ValAla: 6.163 ± 0.491
0.46ValCys: 0.46 ± 0.176
4.323ValAsp: 4.323 ± 0.602
4.047ValGlu: 4.047 ± 0.626
3.036ValPhe: 3.036 ± 0.541
5.059ValGly: 5.059 ± 0.681
1.196ValHis: 1.196 ± 0.435
3.68ValIle: 3.68 ± 0.55
4.047ValLys: 4.047 ± 0.496
3.312ValLeu: 3.312 ± 0.72
2.484ValMet: 2.484 ± 0.345
3.404ValAsn: 3.404 ± 0.53
2.576ValPro: 2.576 ± 0.364
3.128ValGln: 3.128 ± 0.669
4.231ValArg: 4.231 ± 0.657
5.335ValSer: 5.335 ± 0.869
3.036ValThr: 3.036 ± 0.586
4.599ValVal: 4.599 ± 0.889
0.736ValTrp: 0.736 ± 0.245
2.668ValTyr: 2.668 ± 0.613
0.0ValXaa: 0.0 ± 0.0
Trp
0.92TrpAla: 0.92 ± 0.226
0.092TrpCys: 0.092 ± 0.079
0.368TrpAsp: 0.368 ± 0.202
1.288TrpGlu: 1.288 ± 0.321
0.368TrpPhe: 0.368 ± 0.212
1.104TrpGly: 1.104 ± 0.256
0.46TrpHis: 0.46 ± 0.243
0.552TrpIle: 0.552 ± 0.21
1.288TrpLys: 1.288 ± 0.329
1.472TrpLeu: 1.472 ± 0.334
0.184TrpMet: 0.184 ± 0.108
0.46TrpAsn: 0.46 ± 0.205
1.012TrpPro: 1.012 ± 0.295
0.552TrpGln: 0.552 ± 0.262
0.552TrpArg: 0.552 ± 0.217
0.92TrpSer: 0.92 ± 0.376
0.552TrpThr: 0.552 ± 0.214
1.196TrpVal: 1.196 ± 0.387
0.184TrpTrp: 0.184 ± 0.129
0.276TrpTyr: 0.276 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.668TyrAla: 2.668 ± 0.495
0.368TyrCys: 0.368 ± 0.163
2.024TyrAsp: 2.024 ± 0.337
2.116TyrGlu: 2.116 ± 0.442
1.38TyrPhe: 1.38 ± 0.422
3.036TyrGly: 3.036 ± 0.6
0.46TyrHis: 0.46 ± 0.211
1.84TyrIle: 1.84 ± 0.303
2.116TyrLys: 2.116 ± 0.482
2.392TyrLeu: 2.392 ± 0.505
0.736TyrMet: 0.736 ± 0.209
2.116TyrAsn: 2.116 ± 0.476
1.104TyrPro: 1.104 ± 0.331
1.38TyrGln: 1.38 ± 0.298
1.564TyrArg: 1.564 ± 0.435
1.84TyrSer: 1.84 ± 0.415
2.852TyrThr: 2.852 ± 0.486
2.484TyrVal: 2.484 ± 0.422
0.368TyrTrp: 0.368 ± 0.233
0.92TyrTyr: 0.92 ± 0.406
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 23 proteins (10872 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski