Sida yellow vein Madurai virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q17ZR2|Q17ZR2_9GEMI V2 protein OS=Sida yellow vein Madurai virus OX=420255 GN=v2 PE=3 SV=1
MM1 pKa = 7.98WDD3 pKa = 3.34PLEE6 pKa = 4.57NEE8 pKa = 4.32FPEE11 pKa = 4.69TVHH14 pKa = 6.36GFRR17 pKa = 11.84YY18 pKa = 8.53MLAVKK23 pKa = 9.92YY24 pKa = 7.84MQEE27 pKa = 3.89IAKK30 pKa = 8.53STSLVHH36 pKa = 5.96WFRR39 pKa = 11.84VRR41 pKa = 11.84SGSHH45 pKa = 5.26FRR47 pKa = 11.84VEE49 pKa = 4.29VQGLCSSVLQVWRR62 pKa = 11.84FPLQFLPRR70 pKa = 11.84VEE72 pKa = 4.77GSTSTVLAHH81 pKa = 6.97QLLLPLLPQAQGEE94 pKa = 4.38EE95 pKa = 4.27DD96 pKa = 4.85GPTGPCIEE104 pKa = 4.73SPDD107 pKa = 3.46FTEE110 pKa = 4.92CIEE113 pKa = 4.12RR114 pKa = 11.84LMSLRR119 pKa = 11.84AVKK122 pKa = 10.48AHH124 pKa = 5.13VRR126 pKa = 11.84YY127 pKa = 9.69SPSMLKK133 pKa = 9.86TILVTWVRR141 pKa = 11.84LSVYY145 pKa = 10.9LMLLEE150 pKa = 4.13VLGG153 pKa = 4.19

Molecular weight: 17.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q17ZQ9|Q17ZQ9_9GEMI Transcriptional activator protein OS=Sida yellow vein Madurai virus OX=420255 GN=c2 PE=3 SV=1
MM1 pKa = 7.43APSKK5 pKa = 10.57RR6 pKa = 11.84FNIYY10 pKa = 9.69CKK12 pKa = 10.48NYY14 pKa = 8.63FLTYY18 pKa = 9.03PKK20 pKa = 10.53CSLTKK25 pKa = 10.66EE26 pKa = 4.11EE27 pKa = 5.14ALSQIQNLQTPTNKK41 pKa = 10.17KK42 pKa = 9.08YY43 pKa = 10.8IKK45 pKa = 9.86VCKK48 pKa = 9.17EE49 pKa = 3.37LHH51 pKa = 6.47EE52 pKa = 4.83NGEE55 pKa = 4.16PHH57 pKa = 6.66LHH59 pKa = 6.03VLIQFEE65 pKa = 4.78GKK67 pKa = 9.95YY68 pKa = 9.77KK69 pKa = 10.46CQNQRR74 pKa = 11.84FFDD77 pKa = 3.9LVSPNRR83 pKa = 11.84SAHH86 pKa = 5.06FHH88 pKa = 6.56PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.7KK106 pKa = 11.33DD107 pKa = 3.49GDD109 pKa = 3.65TLEE112 pKa = 4.14WGEE115 pKa = 3.84IQIDD119 pKa = 3.71GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 4.41AYY134 pKa = 9.38PPQRR138 pKa = 11.84FTQAVSQRR146 pKa = 11.84LLQYY150 pKa = 10.83LEE152 pKa = 4.55NYY154 pKa = 8.24PLRR157 pKa = 11.84IMFYY161 pKa = 10.62NFIIYY166 pKa = 8.54MLIYY170 pKa = 10.23IGFSHH175 pKa = 7.13LLWRR179 pKa = 11.84FIFLLFLLLLSIKK192 pKa = 10.35FPTNLKK198 pKa = 9.39SGRR201 pKa = 11.84VRR203 pKa = 11.84MSWRR207 pKa = 11.84PLRR210 pKa = 11.84GLGGPSVLWLKK221 pKa = 9.1VTVEE225 pKa = 3.97RR226 pKa = 11.84GRR228 pKa = 11.84RR229 pKa = 11.84CGPGHH234 pKa = 6.43WVHH237 pKa = 7.9IIISVGIWTSAQRR250 pKa = 11.84YY251 pKa = 5.06TVTTLGTTSLMTLIPIISNTLKK273 pKa = 10.86SLWGPRR279 pKa = 11.84EE280 pKa = 3.81TGKK283 pKa = 10.62AIPSTASQFKK293 pKa = 10.44LKK295 pKa = 10.77AGFQQSSFAIQGPIPAIKK313 pKa = 10.58NSWTRR318 pKa = 11.84KK319 pKa = 9.19RR320 pKa = 11.84IPLSRR325 pKa = 11.84PGHH328 pKa = 5.5FKK330 pKa = 10.77CVVRR334 pKa = 11.84YY335 pKa = 7.95PRR337 pKa = 11.84RR338 pKa = 11.84GSLLRR343 pKa = 11.84CPSKK347 pKa = 10.69SSIRR351 pKa = 11.84RR352 pKa = 11.84RR353 pKa = 11.84RR354 pKa = 11.84GKK356 pKa = 10.06YY357 pKa = 9.64AGRR360 pKa = 11.84GG361 pKa = 3.46

Molecular weight: 41.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1167

98

361

194.5

22.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.027 ± 0.436

2.399 ± 0.216

3.085 ± 0.598

3.77 ± 0.736

4.456 ± 0.509

5.998 ± 0.625

3.171 ± 0.781

5.827 ± 0.924

4.542 ± 0.967

8.74 ± 1.007

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.742 ± 0.724

3.599 ± 0.425

5.656 ± 0.391

5.056 ± 0.677

9.169 ± 0.936

9.854 ± 1.366

7.626 ± 1.072

5.398 ± 1.095

1.885 ± 0.252

2.999 ± 0.498
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski