Cyanophage S-RIM14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ahtivirus; unclassified Ahtivirus

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 216 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D7SJR7|A0A1D7SJR7_9CAUD Uncharacterized protein OS=Cyanophage S-RIM14 OX=1278423 GN=LIS021110_020 PE=4 SV=1
MM1 pKa = 7.26TLYY4 pKa = 10.52RR5 pKa = 11.84PNGDD9 pKa = 3.97FGPVCDD15 pKa = 4.74PFEE18 pKa = 4.47EE19 pKa = 4.57PTDD22 pKa = 3.85FSLPILADD30 pKa = 3.71PLVLAFPSPQRR41 pKa = 11.84VLVDD45 pKa = 4.17LEE47 pKa = 4.19GQTVVNVPVWNDD59 pKa = 3.3PGDD62 pKa = 3.74YY63 pKa = 10.19RR64 pKa = 11.84PNGSMGPVCDD74 pKa = 3.66PTSPYY79 pKa = 10.56GPEE82 pKa = 3.9DD83 pKa = 3.78SIGEE87 pKa = 4.03SDD89 pKa = 4.07PRR91 pKa = 11.84VVDD94 pKa = 3.9PQAPPEE100 pKa = 4.57GGGPAYY106 pKa = 10.54SGPTEE111 pKa = 4.28PYY113 pKa = 10.15RR114 pKa = 11.84PLGDD118 pKa = 3.77MGPVCDD124 pKa = 4.57PFVPVNLNPEE134 pKa = 4.34VVTPRR139 pKa = 11.84QDD141 pKa = 3.63LDD143 pKa = 3.85GPAADD148 pKa = 5.31DD149 pKa = 5.34DD150 pKa = 5.14DD151 pKa = 6.71GEE153 pKa = 4.74TYY155 pKa = 10.43PDD157 pKa = 4.65PRR159 pKa = 11.84VQDD162 pKa = 3.72PKK164 pKa = 10.56RR165 pKa = 11.84PSSVPVSIVEE175 pKa = 4.17PPDD178 pKa = 3.38YY179 pKa = 10.93RR180 pKa = 11.84PDD182 pKa = 3.67GPFGPVCDD190 pKa = 4.15PTLDD194 pKa = 3.97PLLTATAGDD203 pKa = 3.79NAIGDD208 pKa = 4.27GNPDD212 pKa = 4.39DD213 pKa = 5.8LNPDD217 pKa = 4.43DD218 pKa = 5.22IYY220 pKa = 11.07PDD222 pKa = 3.24IVNFPAALPNWTNLPDD238 pKa = 3.87SPPTIGLPSTDD249 pKa = 3.68EE250 pKa = 4.3FEE252 pKa = 5.8DD253 pKa = 5.61GIDD256 pKa = 3.69APSVAGVAVSYY267 pKa = 10.93IPFFDD272 pKa = 3.41TSNINNIPASFSFDD286 pKa = 3.8DD287 pKa = 4.29PEE289 pKa = 5.41PDD291 pKa = 3.3KK292 pKa = 11.72GDD294 pKa = 3.96WNWSHH299 pKa = 6.91YY300 pKa = 9.61YY301 pKa = 10.67FNGKK305 pKa = 8.78FFEE308 pKa = 4.27WDD310 pKa = 3.08EE311 pKa = 4.14TDD313 pKa = 3.22TFNGLTGAGLYY324 pKa = 10.9GEE326 pKa = 5.78LIDD329 pKa = 5.66AADD332 pKa = 4.17NDD334 pKa = 4.14VEE336 pKa = 4.73IPDD339 pKa = 3.62GDD341 pKa = 3.58RR342 pKa = 11.84EE343 pKa = 4.35VFQPDD348 pKa = 4.25SYY350 pKa = 11.63CEE352 pKa = 4.13EE353 pKa = 3.97PWGDD357 pKa = 3.72QVLDD361 pKa = 4.64PYY363 pKa = 11.16TDD365 pKa = 3.19TDD367 pKa = 3.82GNVVYY372 pKa = 10.33RR373 pKa = 11.84QVRR376 pKa = 11.84SFPRR380 pKa = 11.84TYY382 pKa = 10.22QVSHH386 pKa = 6.57EE387 pKa = 4.13NFPEE391 pKa = 3.93EE392 pKa = 4.24FAQKK396 pKa = 10.22SAWISQGICSFEE408 pKa = 3.98GKK410 pKa = 7.83TRR412 pKa = 11.84EE413 pKa = 4.46AGDD416 pKa = 3.25AAYY419 pKa = 7.42FTSTITIPDD428 pKa = 3.34TDD430 pKa = 3.9TYY432 pKa = 10.5KK433 pKa = 10.28IRR435 pKa = 11.84YY436 pKa = 8.7KK437 pKa = 10.27FFKK440 pKa = 10.22RR441 pKa = 11.84GEE443 pKa = 3.89IFLDD447 pKa = 3.56YY448 pKa = 10.71FDD450 pKa = 5.3PNKK453 pKa = 8.46TTIEE457 pKa = 4.12LVSEE461 pKa = 4.52AEE463 pKa = 4.42GSASFSGDD471 pKa = 2.94EE472 pKa = 3.96YY473 pKa = 11.24DD474 pKa = 3.73VVSRR478 pKa = 11.84EE479 pKa = 4.05IEE481 pKa = 3.94AGEE484 pKa = 3.91YY485 pKa = 10.88DD486 pKa = 3.74LTTFIEE492 pKa = 4.7NYY494 pKa = 10.64AIGTPQSLWKK504 pKa = 10.41DD505 pKa = 3.59SPAACAIEE513 pKa = 5.13VYY515 pKa = 10.48QGDD518 pKa = 3.95FSIVTGQIPCDD529 pKa = 3.48VGIIEE534 pKa = 4.52SNIPGGASFDD544 pKa = 4.29SNGNIIVTGQCRR556 pKa = 11.84VQFDD560 pKa = 3.75FSYY563 pKa = 11.42DD564 pKa = 4.0DD565 pKa = 4.49NPDD568 pKa = 3.32TYY570 pKa = 10.28GTALGTVSYY579 pKa = 10.43SALNISFTQDD589 pKa = 2.84TQTSTGSARR598 pKa = 11.84EE599 pKa = 4.05TRR601 pKa = 11.84RR602 pKa = 11.84VAQGTYY608 pKa = 8.95QVSLSNANSAGFVLQNNNTEE628 pKa = 3.68ICFFDD633 pKa = 5.95GDD635 pKa = 4.1AQDD638 pKa = 4.61CNALLRR644 pKa = 11.84LTTLDD649 pKa = 3.39STSAGEE655 pKa = 4.88GVTFTCGTVAEE666 pKa = 4.51VEE668 pKa = 3.92LGLRR672 pKa = 11.84YY673 pKa = 10.08DD674 pKa = 5.3DD675 pKa = 5.08NPTTQGLCLSSVTWSGDD692 pKa = 2.91EE693 pKa = 5.26DD694 pKa = 3.48ITLVRR699 pKa = 11.84DD700 pKa = 3.61TNKK703 pKa = 10.22EE704 pKa = 3.97SGSRR708 pKa = 11.84TVRR711 pKa = 11.84KK712 pKa = 9.85DD713 pKa = 3.55LLSGTYY719 pKa = 9.6TMNLVDD725 pKa = 3.76NAGGYY730 pKa = 9.44LVQNPGTTQNDD741 pKa = 4.27SYY743 pKa = 11.42IVLYY747 pKa = 10.83DD748 pKa = 3.67GGGSDD753 pKa = 3.47EE754 pKa = 4.52NGRR757 pKa = 11.84LTCEE761 pKa = 4.18QISATGSSVFSSFDD775 pKa = 3.57SNGNLVITGTRR786 pKa = 11.84GYY788 pKa = 9.29TNDD791 pKa = 3.96FIEE794 pKa = 4.57GFKK797 pKa = 10.7DD798 pKa = 4.91DD799 pKa = 3.81SSVWQQSDD807 pKa = 3.45VGTEE811 pKa = 3.69ILFNATDD818 pKa = 3.58SSKK821 pKa = 11.1GMQVQIGIIPRR832 pKa = 11.84NRR834 pKa = 11.84YY835 pKa = 9.24DD836 pKa = 3.43GNNYY840 pKa = 8.8TISTDD845 pKa = 3.08WAVKK849 pKa = 9.87QIINYY854 pKa = 8.66GVGYY858 pKa = 10.43SVDD861 pKa = 3.77DD862 pKa = 3.68TWNVSYY868 pKa = 10.77TNSATGGTTSAKK880 pKa = 10.35VKK882 pKa = 10.27IFSTRR887 pKa = 11.84KK888 pKa = 9.26GRR890 pKa = 11.84ATVGNLVWSTTNNAVGYY907 pKa = 9.52DD908 pKa = 3.58EE909 pKa = 6.03SFTPFPP915 pKa = 4.71

Molecular weight: 99.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D7SHV3|A0A1D7SHV3_9CAUD Uncharacterized protein OS=Cyanophage S-RIM14 OX=1278423 GN=LIS021110_046 PE=4 SV=1
MM1 pKa = 7.01TRR3 pKa = 11.84VRR5 pKa = 11.84NQVGTWFGTKK15 pKa = 7.43TTLDD19 pKa = 3.88DD20 pKa = 4.16KK21 pKa = 11.76SLGGLSANQKK31 pKa = 10.17RR32 pKa = 11.84EE33 pKa = 3.73VLRR36 pKa = 11.84LIDD39 pKa = 4.26RR40 pKa = 11.84AIDD43 pKa = 3.35NHH45 pKa = 5.87NRR47 pKa = 11.84TATLVSASIGGVLLFFYY64 pKa = 10.68AHH66 pKa = 6.07GVVSIVDD73 pKa = 3.65RR74 pKa = 11.84TANN77 pKa = 3.21

Molecular weight: 8.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

216

0

216

57942

34

6198

268.3

29.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.902 ± 0.246

0.951 ± 0.1

6.765 ± 0.151

6.047 ± 0.29

4.28 ± 0.1

7.956 ± 0.37

1.436 ± 0.123

6.325 ± 0.198

5.645 ± 0.388

7.042 ± 0.172

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.188 ± 0.194

5.726 ± 0.157

4.025 ± 0.15

3.862 ± 0.097

4.237 ± 0.198

7.159 ± 0.192

7.397 ± 0.462

6.615 ± 0.168

1.179 ± 0.093

4.261 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski