Amino acid dipepetide frequency for Cyanophage S-RIM14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.213AlaAla: 6.213 ± 0.489
0.397AlaCys: 0.397 ± 0.103
4.021AlaAsp: 4.021 ± 0.317
3.538AlaGlu: 3.538 ± 0.281
2.917AlaPhe: 2.917 ± 0.23
6.472AlaGly: 6.472 ± 0.541
0.932AlaHis: 0.932 ± 0.138
4.228AlaIle: 4.228 ± 0.368
3.883AlaLys: 3.883 ± 0.378
4.66AlaLeu: 4.66 ± 0.304
1.484AlaMet: 1.484 ± 0.169
4.09AlaAsn: 4.09 ± 0.335
2.71AlaPro: 2.71 ± 0.22
2.468AlaGln: 2.468 ± 0.234
2.71AlaArg: 2.71 ± 0.273
4.815AlaSer: 4.815 ± 0.414
5.695AlaThr: 5.695 ± 0.528
4.763AlaVal: 4.763 ± 0.342
0.621AlaTrp: 0.621 ± 0.133
2.399AlaTyr: 2.399 ± 0.236
0.0AlaXaa: 0.0 ± 0.0
Cys
0.587CysAla: 0.587 ± 0.105
0.138CysCys: 0.138 ± 0.047
0.811CysAsp: 0.811 ± 0.173
0.501CysGlu: 0.501 ± 0.117
0.501CysPhe: 0.501 ± 0.123
0.621CysGly: 0.621 ± 0.135
0.293CysHis: 0.293 ± 0.08
0.69CysIle: 0.69 ± 0.131
0.587CysLys: 0.587 ± 0.108
0.466CysLeu: 0.466 ± 0.096
0.276CysMet: 0.276 ± 0.071
0.501CysAsn: 0.501 ± 0.112
0.345CysPro: 0.345 ± 0.086
0.345CysGln: 0.345 ± 0.085
0.466CysArg: 0.466 ± 0.103
0.742CysSer: 0.742 ± 0.133
0.431CysThr: 0.431 ± 0.081
0.501CysVal: 0.501 ± 0.111
0.155CysTrp: 0.155 ± 0.072
0.552CysTyr: 0.552 ± 0.098
0.0CysXaa: 0.0 ± 0.0
Asp
5.454AspAla: 5.454 ± 0.392
0.811AspCys: 0.811 ± 0.144
4.694AspAsp: 4.694 ± 0.411
3.97AspGlu: 3.97 ± 0.34
2.641AspPhe: 2.641 ± 0.241
5.73AspGly: 5.73 ± 0.489
1.053AspHis: 1.053 ± 0.141
4.108AspIle: 4.108 ± 0.265
3.4AspLys: 3.4 ± 0.354
4.798AspLeu: 4.798 ± 0.305
1.709AspMet: 1.709 ± 0.199
3.607AspAsn: 3.607 ± 0.277
3.262AspPro: 3.262 ± 0.304
2.434AspGln: 2.434 ± 0.212
2.761AspArg: 2.761 ± 0.264
4.453AspSer: 4.453 ± 0.315
4.591AspThr: 4.591 ± 0.399
4.056AspVal: 4.056 ± 0.289
0.897AspTrp: 0.897 ± 0.122
3.21AspTyr: 3.21 ± 0.248
0.0AspXaa: 0.0 ± 0.0
Glu
3.435GluAla: 3.435 ± 0.228
0.915GluCys: 0.915 ± 0.149
3.659GluAsp: 3.659 ± 0.257
4.729GluGlu: 4.729 ± 0.55
3.072GluPhe: 3.072 ± 0.264
3.97GluGly: 3.97 ± 0.32
0.794GluHis: 0.794 ± 0.143
3.883GluIle: 3.883 ± 0.308
3.538GluLys: 3.538 ± 0.357
4.902GluLeu: 4.902 ± 0.272
1.881GluMet: 1.881 ± 0.272
3.141GluAsn: 3.141 ± 0.249
2.019GluPro: 2.019 ± 0.175
2.485GluGln: 2.485 ± 0.251
2.692GluArg: 2.692 ± 0.316
3.814GluSer: 3.814 ± 0.29
3.883GluThr: 3.883 ± 0.28
4.332GluVal: 4.332 ± 0.281
0.811GluTrp: 0.811 ± 0.161
2.52GluTyr: 2.52 ± 0.243
0.0GluXaa: 0.0 ± 0.0
Phe
2.744PheAla: 2.744 ± 0.223
0.604PheCys: 0.604 ± 0.111
3.573PheAsp: 3.573 ± 0.242
2.399PheGlu: 2.399 ± 0.188
1.847PhePhe: 1.847 ± 0.14
3.365PheGly: 3.365 ± 0.237
0.621PheHis: 0.621 ± 0.127
2.779PheIle: 2.779 ± 0.231
1.829PheLys: 1.829 ± 0.229
3.227PheLeu: 3.227 ± 0.307
0.863PheMet: 0.863 ± 0.154
2.382PheAsn: 2.382 ± 0.228
1.812PhePro: 1.812 ± 0.235
1.484PheGln: 1.484 ± 0.13
1.778PheArg: 1.778 ± 0.177
3.365PheSer: 3.365 ± 0.251
3.072PheThr: 3.072 ± 0.299
2.951PheVal: 2.951 ± 0.25
0.362PheTrp: 0.362 ± 0.084
1.743PheTyr: 1.743 ± 0.124
0.0PheXaa: 0.0 ± 0.0
Gly
6.127GlyAla: 6.127 ± 0.607
0.759GlyCys: 0.759 ± 0.142
4.763GlyAsp: 4.763 ± 0.386
4.142GlyGlu: 4.142 ± 0.318
3.055GlyPhe: 3.055 ± 0.229
8.681GlyGly: 8.681 ± 1.261
0.846GlyHis: 0.846 ± 0.132
4.884GlyIle: 4.884 ± 0.413
3.849GlyLys: 3.849 ± 0.361
4.746GlyLeu: 4.746 ± 0.36
1.76GlyMet: 1.76 ± 0.258
4.487GlyAsn: 4.487 ± 0.336
2.209GlyPro: 2.209 ± 0.257
2.9GlyGln: 2.9 ± 0.221
3.107GlyArg: 3.107 ± 0.244
6.662GlySer: 6.662 ± 0.542
7.18GlyThr: 7.18 ± 0.814
4.902GlyVal: 4.902 ± 0.297
1.139GlyTrp: 1.139 ± 0.184
3.365GlyTyr: 3.365 ± 0.353
0.0GlyXaa: 0.0 ± 0.0
His
0.828HisAla: 0.828 ± 0.158
0.19HisCys: 0.19 ± 0.071
1.156HisAsp: 1.156 ± 0.17
0.863HisGlu: 0.863 ± 0.136
0.673HisPhe: 0.673 ± 0.129
1.07HisGly: 1.07 ± 0.146
0.242HisHis: 0.242 ± 0.07
0.846HisIle: 0.846 ± 0.129
0.777HisLys: 0.777 ± 0.135
1.07HisLeu: 1.07 ± 0.157
0.38HisMet: 0.38 ± 0.091
0.725HisAsn: 0.725 ± 0.155
0.88HisPro: 0.88 ± 0.13
0.466HisGln: 0.466 ± 0.083
0.449HisArg: 0.449 ± 0.106
0.915HisSer: 0.915 ± 0.117
1.053HisThr: 1.053 ± 0.154
0.673HisVal: 0.673 ± 0.108
0.207HisTrp: 0.207 ± 0.056
0.897HisTyr: 0.897 ± 0.167
0.0HisXaa: 0.0 ± 0.0
Ile
4.108IleAla: 4.108 ± 0.298
0.535IleCys: 0.535 ± 0.112
5.143IleAsp: 5.143 ± 0.322
4.574IleGlu: 4.574 ± 0.283
2.416IlePhe: 2.416 ± 0.197
4.436IleGly: 4.436 ± 0.267
0.777IleHis: 0.777 ± 0.137
3.797IleIle: 3.797 ± 0.345
3.676IleLys: 3.676 ± 0.327
4.436IleLeu: 4.436 ± 0.324
1.363IleMet: 1.363 ± 0.205
3.521IleAsn: 3.521 ± 0.273
2.744IlePro: 2.744 ± 0.282
2.744IleGln: 2.744 ± 0.251
2.503IleArg: 2.503 ± 0.228
4.487IleSer: 4.487 ± 0.342
5.368IleThr: 5.368 ± 0.622
3.728IleVal: 3.728 ± 0.317
0.604IleTrp: 0.604 ± 0.118
2.295IleTyr: 2.295 ± 0.22
0.0IleXaa: 0.0 ± 0.0
Lys
3.245LysAla: 3.245 ± 0.39
0.673LysCys: 0.673 ± 0.134
3.383LysAsp: 3.383 ± 0.325
3.952LysGlu: 3.952 ± 0.453
2.813LysPhe: 2.813 ± 0.233
3.245LysGly: 3.245 ± 0.408
0.777LysHis: 0.777 ± 0.143
4.125LysIle: 4.125 ± 0.398
4.487LysLys: 4.487 ± 0.591
4.09LysLeu: 4.09 ± 0.346
1.467LysMet: 1.467 ± 0.202
2.882LysAsn: 2.882 ± 0.28
2.071LysPro: 2.071 ± 0.251
2.399LysGln: 2.399 ± 0.301
2.434LysArg: 2.434 ± 0.278
3.78LysSer: 3.78 ± 0.369
3.089LysThr: 3.089 ± 0.251
4.073LysVal: 4.073 ± 0.285
0.777LysTrp: 0.777 ± 0.128
2.692LysTyr: 2.692 ± 0.353
0.0LysXaa: 0.0 ± 0.0
Leu
4.85LeuAla: 4.85 ± 0.313
0.759LeuCys: 0.759 ± 0.163
5.885LeuAsp: 5.885 ± 0.293
4.021LeuGlu: 4.021 ± 0.343
3.02LeuPhe: 3.02 ± 0.231
4.902LeuGly: 4.902 ± 0.335
1.381LeuHis: 1.381 ± 0.188
3.762LeuIle: 3.762 ± 0.272
4.263LeuLys: 4.263 ± 0.416
4.781LeuLeu: 4.781 ± 0.362
1.467LeuMet: 1.467 ± 0.163
4.263LeuAsn: 4.263 ± 0.271
2.813LeuPro: 2.813 ± 0.3
3.193LeuGln: 3.193 ± 0.268
3.365LeuArg: 3.365 ± 0.261
4.746LeuSer: 4.746 ± 0.291
5.229LeuThr: 5.229 ± 0.495
4.125LeuVal: 4.125 ± 0.269
0.742LeuTrp: 0.742 ± 0.132
2.848LeuTyr: 2.848 ± 0.295
0.0LeuXaa: 0.0 ± 0.0
Met
1.64MetAla: 1.64 ± 0.207
0.086MetCys: 0.086 ± 0.04
1.191MetAsp: 1.191 ± 0.173
1.467MetGlu: 1.467 ± 0.188
0.777MetPhe: 0.777 ± 0.16
1.294MetGly: 1.294 ± 0.162
0.414MetHis: 0.414 ± 0.111
1.122MetIle: 1.122 ± 0.144
1.76MetLys: 1.76 ± 0.282
1.605MetLeu: 1.605 ± 0.217
0.742MetMet: 0.742 ± 0.133
1.415MetAsn: 1.415 ± 0.2
1.053MetPro: 1.053 ± 0.129
0.621MetGln: 0.621 ± 0.121
1.018MetArg: 1.018 ± 0.165
1.691MetSer: 1.691 ± 0.248
1.536MetThr: 1.536 ± 0.224
1.346MetVal: 1.346 ± 0.172
0.328MetTrp: 0.328 ± 0.081
0.777MetTyr: 0.777 ± 0.129
0.0MetXaa: 0.0 ± 0.0
Asn
3.901AsnAla: 3.901 ± 0.344
0.552AsnCys: 0.552 ± 0.092
3.055AsnAsp: 3.055 ± 0.256
2.848AsnGlu: 2.848 ± 0.28
2.727AsnPhe: 2.727 ± 0.239
4.522AsnGly: 4.522 ± 0.378
0.863AsnHis: 0.863 ± 0.123
4.004AsnIle: 4.004 ± 0.273
2.969AsnLys: 2.969 ± 0.27
4.746AsnLeu: 4.746 ± 0.454
0.915AsnMet: 0.915 ± 0.182
3.279AsnAsn: 3.279 ± 0.281
2.951AsnPro: 2.951 ± 0.213
2.295AsnGln: 2.295 ± 0.203
2.796AsnArg: 2.796 ± 0.301
4.056AsnSer: 4.056 ± 0.343
3.607AsnThr: 3.607 ± 0.362
3.797AsnVal: 3.797 ± 0.33
0.759AsnTrp: 0.759 ± 0.109
2.623AsnTyr: 2.623 ± 0.219
0.0AsnXaa: 0.0 ± 0.0
Pro
2.727ProAla: 2.727 ± 0.247
0.414ProCys: 0.414 ± 0.13
2.848ProAsp: 2.848 ± 0.276
2.813ProGlu: 2.813 ± 0.249
1.778ProPhe: 1.778 ± 0.178
3.227ProGly: 3.227 ± 0.291
0.742ProHis: 0.742 ± 0.11
2.175ProIle: 2.175 ± 0.288
2.106ProLys: 2.106 ± 0.294
2.226ProLeu: 2.226 ± 0.187
0.587ProMet: 0.587 ± 0.113
2.054ProAsn: 2.054 ± 0.165
1.933ProPro: 1.933 ± 0.222
1.657ProGln: 1.657 ± 0.148
1.795ProArg: 1.795 ± 0.186
3.003ProSer: 3.003 ± 0.25
3.124ProThr: 3.124 ± 0.271
2.9ProVal: 2.9 ± 0.23
0.466ProTrp: 0.466 ± 0.105
1.674ProTyr: 1.674 ± 0.171
0.0ProXaa: 0.0 ± 0.0
Gln
2.019GlnAla: 2.019 ± 0.166
0.276GlnCys: 0.276 ± 0.064
2.14GlnAsp: 2.14 ± 0.245
2.52GlnGlu: 2.52 ± 0.253
1.864GlnPhe: 1.864 ± 0.193
2.503GlnGly: 2.503 ± 0.245
0.535GlnHis: 0.535 ± 0.094
3.176GlnIle: 3.176 ± 0.252
2.347GlnLys: 2.347 ± 0.382
2.951GlnLeu: 2.951 ± 0.263
0.967GlnMet: 0.967 ± 0.158
2.192GlnAsn: 2.192 ± 0.169
1.191GlnPro: 1.191 ± 0.16
1.881GlnGln: 1.881 ± 0.239
1.778GlnArg: 1.778 ± 0.158
2.641GlnSer: 2.641 ± 0.199
2.451GlnThr: 2.451 ± 0.227
2.623GlnVal: 2.623 ± 0.171
0.535GlnTrp: 0.535 ± 0.099
2.037GlnTyr: 2.037 ± 0.19
0.0GlnXaa: 0.0 ± 0.0
Arg
2.744ArgAla: 2.744 ± 0.2
0.293ArgCys: 0.293 ± 0.074
2.451ArgAsp: 2.451 ± 0.268
2.606ArgGlu: 2.606 ± 0.34
1.847ArgPhe: 1.847 ± 0.143
3.055ArgGly: 3.055 ± 0.211
0.759ArgHis: 0.759 ± 0.118
2.641ArgIle: 2.641 ± 0.204
2.9ArgLys: 2.9 ± 0.371
3.141ArgLeu: 3.141 ± 0.251
1.036ArgMet: 1.036 ± 0.15
2.261ArgAsn: 2.261 ± 0.217
1.605ArgPro: 1.605 ± 0.206
1.502ArgGln: 1.502 ± 0.167
2.295ArgArg: 2.295 ± 0.334
2.641ArgSer: 2.641 ± 0.237
2.589ArgThr: 2.589 ± 0.202
3.314ArgVal: 3.314 ± 0.255
0.552ArgTrp: 0.552 ± 0.104
2.14ArgTyr: 2.14 ± 0.217
0.0ArgXaa: 0.0 ± 0.0
Ser
5.195SerAla: 5.195 ± 0.447
0.397SerCys: 0.397 ± 0.095
4.453SerAsp: 4.453 ± 0.312
3.745SerGlu: 3.745 ± 0.303
3.486SerPhe: 3.486 ± 0.27
7.197SerGly: 7.197 ± 0.655
0.915SerHis: 0.915 ± 0.139
4.522SerIle: 4.522 ± 0.326
4.004SerLys: 4.004 ± 0.34
4.66SerLeu: 4.66 ± 0.27
1.467SerMet: 1.467 ± 0.178
4.487SerAsn: 4.487 ± 0.294
2.434SerPro: 2.434 ± 0.234
2.399SerGln: 2.399 ± 0.211
2.554SerArg: 2.554 ± 0.193
5.73SerSer: 5.73 ± 0.616
5.368SerThr: 5.368 ± 0.476
4.746SerVal: 4.746 ± 0.295
0.759SerTrp: 0.759 ± 0.111
3.072SerTyr: 3.072 ± 0.245
0.0SerXaa: 0.0 ± 0.0
Thr
5.575ThrAla: 5.575 ± 0.575
0.57ThrCys: 0.57 ± 0.1
4.608ThrAsp: 4.608 ± 0.385
3.745ThrGlu: 3.745 ± 0.204
2.934ThrPhe: 2.934 ± 0.269
7.111ThrGly: 7.111 ± 0.65
0.794ThrHis: 0.794 ± 0.104
5.247ThrIle: 5.247 ± 0.561
3.4ThrLys: 3.4 ± 0.289
5.851ThrLeu: 5.851 ± 0.439
1.07ThrMet: 1.07 ± 0.154
4.505ThrAsn: 4.505 ± 0.467
2.934ThrPro: 2.934 ± 0.236
2.572ThrGln: 2.572 ± 0.212
2.589ThrArg: 2.589 ± 0.225
5.281ThrSer: 5.281 ± 0.529
6.489ThrThr: 6.489 ± 0.842
5.195ThrVal: 5.195 ± 0.655
0.828ThrTrp: 0.828 ± 0.131
2.675ThrTyr: 2.675 ± 0.237
0.0ThrXaa: 0.0 ± 0.0
Val
4.47ValAla: 4.47 ± 0.335
0.501ValCys: 0.501 ± 0.097
5.385ValAsp: 5.385 ± 0.582
4.436ValGlu: 4.436 ± 0.267
2.226ValPhe: 2.226 ± 0.182
4.677ValGly: 4.677 ± 0.262
0.708ValHis: 0.708 ± 0.148
3.831ValIle: 3.831 ± 0.36
3.348ValLys: 3.348 ± 0.246
4.367ValLeu: 4.367 ± 0.287
1.312ValMet: 1.312 ± 0.187
3.607ValAsn: 3.607 ± 0.376
3.365ValPro: 3.365 ± 0.299
2.658ValGln: 2.658 ± 0.217
2.744ValArg: 2.744 ± 0.249
5.022ValSer: 5.022 ± 0.398
5.747ValThr: 5.747 ± 0.566
4.47ValVal: 4.47 ± 0.255
0.656ValTrp: 0.656 ± 0.122
2.623ValTyr: 2.623 ± 0.186
0.0ValXaa: 0.0 ± 0.0
Trp
0.673TrpAla: 0.673 ± 0.101
0.155TrpCys: 0.155 ± 0.058
0.708TrpAsp: 0.708 ± 0.096
0.863TrpGlu: 0.863 ± 0.148
0.57TrpPhe: 0.57 ± 0.121
0.777TrpGly: 0.777 ± 0.123
0.311TrpHis: 0.311 ± 0.079
0.69TrpIle: 0.69 ± 0.146
0.863TrpLys: 0.863 ± 0.192
0.673TrpLeu: 0.673 ± 0.124
0.293TrpMet: 0.293 ± 0.07
0.932TrpAsn: 0.932 ± 0.136
0.242TrpPro: 0.242 ± 0.072
0.552TrpGln: 0.552 ± 0.122
0.518TrpArg: 0.518 ± 0.084
1.018TrpSer: 1.018 ± 0.141
0.794TrpThr: 0.794 ± 0.119
0.673TrpVal: 0.673 ± 0.119
0.104TrpTrp: 0.104 ± 0.046
0.38TrpTyr: 0.38 ± 0.08
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.485TyrAla: 2.485 ± 0.197
0.483TyrCys: 0.483 ± 0.09
3.728TyrAsp: 3.728 ± 0.337
2.744TyrGlu: 2.744 ± 0.262
1.64TyrPhe: 1.64 ± 0.164
2.71TyrGly: 2.71 ± 0.186
0.604TyrHis: 0.604 ± 0.101
2.744TyrIle: 2.744 ± 0.266
2.468TyrLys: 2.468 ± 0.22
3.02TyrLeu: 3.02 ± 0.263
0.828TyrMet: 0.828 ± 0.147
2.934TyrAsn: 2.934 ± 0.257
1.674TyrPro: 1.674 ± 0.195
1.571TyrGln: 1.571 ± 0.156
2.019TyrArg: 2.019 ± 0.201
2.727TyrSer: 2.727 ± 0.228
2.675TyrThr: 2.675 ± 0.363
2.986TyrVal: 2.986 ± 0.229
0.483TyrTrp: 0.483 ± 0.104
2.088TyrTyr: 2.088 ± 0.197
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 216 proteins (57942 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski