Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Francisellaceae; Francisella; Francisella tularensis; Francisella tularensis subsp. tularensis

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1528 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5NGS2|Q5NGS2_FRATT Hypothetical lipoprotein OS=Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) OX=177416 GN=FTT_0760c PE=4 SV=1
MM1 pKa = 7.55TEE3 pKa = 3.52INQNYY8 pKa = 10.18LYY10 pKa = 9.9TDD12 pKa = 3.32SNLWVEE18 pKa = 4.41VKK20 pKa = 11.02GNIARR25 pKa = 11.84VGIDD29 pKa = 4.98DD30 pKa = 3.91YY31 pKa = 11.95SQDD34 pKa = 3.35EE35 pKa = 4.49FGEE38 pKa = 4.18IVYY41 pKa = 10.64VDD43 pKa = 4.09LPKK46 pKa = 10.73LEE48 pKa = 4.01QEE50 pKa = 4.28YY51 pKa = 11.14AKK53 pKa = 10.69DD54 pKa = 4.12DD55 pKa = 4.71EE56 pKa = 4.59ICVIEE61 pKa = 4.26SVKK64 pKa = 9.81TASDD68 pKa = 3.26IYY70 pKa = 10.29TPLSGKK76 pKa = 7.53VVKK79 pKa = 10.54INQQLLDD86 pKa = 3.84NPKK89 pKa = 10.32LINEE93 pKa = 4.12SCYY96 pKa = 10.99DD97 pKa = 3.71KK98 pKa = 11.37GWLFEE103 pKa = 4.45IEE105 pKa = 4.3IADD108 pKa = 4.08LDD110 pKa = 4.12EE111 pKa = 5.33LKK113 pKa = 11.12SLINPQDD120 pKa = 3.56YY121 pKa = 10.57QSYY124 pKa = 10.24VSS126 pKa = 3.85

Molecular weight: 14.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5NI75|Q5NI75_FRATT ABC transporter ATP-binding protein OS=Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) OX=177416 GN=FTT_0208c PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 6.05GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.53TLSGRR28 pKa = 11.84KK29 pKa = 8.83VIRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.44GRR39 pKa = 11.84AKK41 pKa = 10.44LAAA44 pKa = 4.52

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1528

0

1528

474277

32

1422

310.4

34.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.042 ± 0.059

1.097 ± 0.016

5.579 ± 0.053

5.54 ± 0.069

4.911 ± 0.069

5.807 ± 0.061

1.752 ± 0.028

9.294 ± 0.076

7.92 ± 0.061

9.688 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.03

5.811 ± 0.065

3.063 ± 0.031

3.774 ± 0.043

3.297 ± 0.045

6.672 ± 0.047

5.112 ± 0.035

6.342 ± 0.052

0.874 ± 0.018

4.097 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski