Eubacterium sp. 14-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; unclassified Eubacterium

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3773 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0J1P8|S0J1P8_9FIRM Glyco_trans_2-like domain-containing protein OS=Eubacterium sp. 14-2 OX=1235790 GN=C805_03404 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.37KK3 pKa = 10.2KK4 pKa = 10.48LLAALLCVAMSASMLAGCGNNSGGNNSANAGDD36 pKa = 4.64DD37 pKa = 3.7KK38 pKa = 11.75QEE40 pKa = 4.31TGDD43 pKa = 4.46DD44 pKa = 3.72AAGDD48 pKa = 3.89DD49 pKa = 5.15AEE51 pKa = 6.14AGDD54 pKa = 4.44DD55 pKa = 4.87AEE57 pKa = 6.21AGDD60 pKa = 4.43DD61 pKa = 3.94AAAGDD66 pKa = 4.57EE67 pKa = 4.14NTLTVWCWDD76 pKa = 3.48PKK78 pKa = 9.91FNIYY82 pKa = 11.02AMEE85 pKa = 4.21EE86 pKa = 3.89AGKK89 pKa = 10.27LYY91 pKa = 10.58QADD94 pKa = 3.47HH95 pKa = 7.26PEE97 pKa = 3.88FNINVVEE104 pKa = 4.43TPWDD108 pKa = 4.73DD109 pKa = 3.35IQKK112 pKa = 10.79KK113 pKa = 8.07LTTAATGGALDD124 pKa = 4.0TLPDD128 pKa = 3.33IMLVQDD134 pKa = 3.7NAFQKK139 pKa = 10.82NVISFPEE146 pKa = 4.33AFTDD150 pKa = 3.9LTDD153 pKa = 3.59SGVDD157 pKa = 3.74FSQFGEE163 pKa = 4.06AKK165 pKa = 8.02TAYY168 pKa = 10.1SVVEE172 pKa = 4.01GKK174 pKa = 10.46NYY176 pKa = 10.08GVPFDD181 pKa = 4.06NGTVVACYY189 pKa = 8.94RR190 pKa = 11.84TDD192 pKa = 3.32VLEE195 pKa = 4.31EE196 pKa = 4.04AGFTVDD202 pKa = 5.89DD203 pKa = 4.27FTDD206 pKa = 3.75ITWDD210 pKa = 3.34EE211 pKa = 4.09YY212 pKa = 11.31LEE214 pKa = 4.3KK215 pKa = 10.96GKK217 pKa = 10.78VVLEE221 pKa = 4.15KK222 pKa = 10.13TGQPLLSCQSTEE234 pKa = 3.74SDD236 pKa = 4.16VIMMMLQSCGASLFDD251 pKa = 6.17DD252 pKa = 4.01EE253 pKa = 5.94GNPNMVGNDD262 pKa = 3.35SLKK265 pKa = 10.83KK266 pKa = 10.77VMEE269 pKa = 4.7TYY271 pKa = 9.98QQLVEE276 pKa = 4.19TGVCKK281 pKa = 10.46LVNSWDD287 pKa = 4.1EE288 pKa = 4.08YY289 pKa = 10.34VQTLTTGTVAGTINGCWIMASIQSAEE315 pKa = 4.11DD316 pKa = 3.54QSGKK320 pKa = 9.23WALTNMPSLVGVEE333 pKa = 4.18GATNYY338 pKa = 10.69SNNGGSSWAITSNCKK353 pKa = 10.19NMEE356 pKa = 4.14LAKK359 pKa = 10.78DD360 pKa = 3.76FLKK363 pKa = 10.13STFAGSVEE371 pKa = 4.42LYY373 pKa = 10.98EE374 pKa = 5.11NILPSSGALATYY386 pKa = 10.28LPAGDD391 pKa = 3.8SDD393 pKa = 4.89VYY395 pKa = 11.35AEE397 pKa = 4.09PSEE400 pKa = 4.89FYY402 pKa = 11.11GGDD405 pKa = 3.22AVFKK409 pKa = 10.69KK410 pKa = 8.7ITEE413 pKa = 4.27YY414 pKa = 11.16ASKK417 pKa = 10.7CPSNNTGVYY426 pKa = 9.86YY427 pKa = 10.68YY428 pKa = 9.61EE429 pKa = 4.5ARR431 pKa = 11.84DD432 pKa = 4.04AIATAIQNVVGGSDD446 pKa = 3.1IDD448 pKa = 4.05SEE450 pKa = 4.98LEE452 pKa = 3.78IAQGTVEE459 pKa = 5.08GLMEE463 pKa = 3.89QQ464 pKa = 3.7

Molecular weight: 49.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0JJ38|S0JJ38_9FIRM HTH cro/C1-type domain-containing protein OS=Eubacterium sp. 14-2 OX=1235790 GN=C805_01104 PE=4 SV=1
MM1 pKa = 7.84AGMVLRR7 pKa = 11.84KK8 pKa = 8.44TGVVLLTILKK18 pKa = 9.45WILQAALVALKK29 pKa = 10.49LVLGIAKK36 pKa = 10.15LFLLLLSLVMRR47 pKa = 11.84IVLTMIGVSVGRR59 pKa = 11.84RR60 pKa = 3.23

Molecular weight: 6.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3773

0

3773

1163219

26

3240

308.3

34.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.176 ± 0.045

1.515 ± 0.019

5.234 ± 0.026

8.517 ± 0.047

4.072 ± 0.025

7.143 ± 0.039

1.729 ± 0.019

6.969 ± 0.035

6.621 ± 0.039

8.965 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.146 ± 0.022

4.273 ± 0.029

3.25 ± 0.026

3.668 ± 0.029

4.962 ± 0.039

5.874 ± 0.037

5.21 ± 0.038

6.673 ± 0.032

0.93 ± 0.014

4.073 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski