Amino acid dipepetide frequency for Eubacterium sp. 14-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.327AlaAla: 7.327 ± 0.118	1.033AlaCys: 1.033 ± 0.033	4.268AlaAsp: 4.268 ± 0.072	6.247AlaGlu: 6.247 ± 0.083	2.848AlaPhe: 2.848 ± 0.056	6.55AlaGly: 6.55 ± 0.095	1.007AlaHis: 1.007 ± 0.028	4.186AlaIle: 4.186 ± 0.057	3.919AlaLys: 3.919 ± 0.07	6.25AlaLeu: 6.25 ± 0.08	2.272AlaMet: 2.272 ± 0.048	2.234AlaAsn: 2.234 ± 0.05	1.858AlaPro: 1.858 ± 0.047	2.251AlaGln: 2.251 ± 0.049	3.45AlaArg: 3.45 ± 0.054	3.835AlaSer: 3.835 ± 0.075	2.687AlaThr: 2.687 ± 0.064	6.393AlaVal: 6.393 ± 0.086	0.649AlaTrp: 0.649 ± 0.023	2.499AlaTyr: 2.499 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.952CysAla: 0.952 ± 0.031	0.297CysCys: 0.297 ± 0.017	0.715CysAsp: 0.715 ± 0.025	0.957CysGlu: 0.957 ± 0.027	0.675CysPhe: 0.675 ± 0.024	1.494CysGly: 1.494 ± 0.036	0.322CysHis: 0.322 ± 0.018	1.06CysIle: 1.06 ± 0.034	0.731CysLys: 0.731 ± 0.026	1.244CysLeu: 1.244 ± 0.033	0.535CysMet: 0.535 ± 0.023	0.55CysAsn: 0.55 ± 0.022	0.646CysPro: 0.646 ± 0.027	0.504CysGln: 0.504 ± 0.023	0.971CysArg: 0.971 ± 0.029	1.022CysSer: 1.022 ± 0.033	0.731CysThr: 0.731 ± 0.024	0.995CysVal: 0.995 ± 0.03	0.131CysTrp: 0.131 ± 0.011	0.622CysTyr: 0.622 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	3.422AspAla: 3.422 ± 0.065	0.871AspCys: 0.871 ± 0.03	2.407AspAsp: 2.407 ± 0.057	4.09AspGlu: 4.09 ± 0.065	2.611AspPhe: 2.611 ± 0.046	4.137AspGly: 4.137 ± 0.073	0.76AspHis: 0.76 ± 0.026	4.406AspIle: 4.406 ± 0.067	3.04AspLys: 3.04 ± 0.061	4.28AspLeu: 4.28 ± 0.066	1.875AspMet: 1.875 ± 0.041	2.086AspAsn: 2.086 ± 0.046	1.344AspPro: 1.344 ± 0.036	1.156AspGln: 1.156 ± 0.033	2.772AspArg: 2.772 ± 0.048	3.219AspSer: 3.219 ± 0.061	3.015AspThr: 3.015 ± 0.052	3.525AspVal: 3.525 ± 0.058	0.569AspTrp: 0.569 ± 0.025	2.761AspTyr: 2.761 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	5.658GluAla: 5.658 ± 0.082	0.877GluCys: 0.877 ± 0.028	4.475GluAsp: 4.475 ± 0.066	8.977GluGlu: 8.977 ± 0.123	3.001GluPhe: 3.001 ± 0.053	4.913GluGly: 4.913 ± 0.083	1.695GluHis: 1.695 ± 0.046	6.141GluIle: 6.141 ± 0.085	7.573GluLys: 7.573 ± 0.087	7.669GluLeu: 7.669 ± 0.091	2.557GluMet: 2.557 ± 0.046	4.528GluAsn: 4.528 ± 0.069	2.297GluPro: 2.297 ± 0.068	4.032GluGln: 4.032 ± 0.084	3.897GluArg: 3.897 ± 0.067	3.863GluSer: 3.863 ± 0.065	4.319GluThr: 4.319 ± 0.064	4.484GluVal: 4.484 ± 0.067	0.742GluTrp: 0.742 ± 0.026	3.473GluTyr: 3.473 ± 0.057	0.0GluXaa: 0.0 ± 0.0
Phe	2.86PheAla: 2.86 ± 0.052	0.857PheCys: 0.857 ± 0.027	2.297PheAsp: 2.297 ± 0.048	2.768PheGlu: 2.768 ± 0.051	1.823PhePhe: 1.823 ± 0.047	2.885PheGly: 2.885 ± 0.055	0.93PheHis: 0.93 ± 0.03	2.454PheIle: 2.454 ± 0.051	1.666PheLys: 1.666 ± 0.042	4.265PheLeu: 4.265 ± 0.076	1.166PheMet: 1.166 ± 0.032	1.351PheAsn: 1.351 ± 0.039	1.419PhePro: 1.419 ± 0.038	1.721PheGln: 1.721 ± 0.038	2.058PheArg: 2.058 ± 0.047	3.06PheSer: 3.06 ± 0.057	2.246PheThr: 2.246 ± 0.043	2.674PheVal: 2.674 ± 0.057	0.42PheTrp: 0.42 ± 0.02	1.803PheTyr: 1.803 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	4.961GlyAla: 4.961 ± 0.073	1.253GlyCys: 1.253 ± 0.031	3.31GlyAsp: 3.31 ± 0.062	5.355GlyGlu: 5.355 ± 0.077	3.08GlyPhe: 3.08 ± 0.049	4.655GlyGly: 4.655 ± 0.087	1.175GlyHis: 1.175 ± 0.035	5.989GlyIle: 5.989 ± 0.075	5.959GlyLys: 5.959 ± 0.076	5.61GlyLeu: 5.61 ± 0.08	2.68GlyMet: 2.68 ± 0.048	3.414GlyAsn: 3.414 ± 0.055	1.225GlyPro: 1.225 ± 0.036	2.333GlyGln: 2.333 ± 0.049	3.539GlyArg: 3.539 ± 0.062	3.984GlySer: 3.984 ± 0.069	4.379GlyThr: 4.379 ± 0.075	4.583GlyVal: 4.583 ± 0.072	0.713GlyTrp: 0.713 ± 0.026	3.234GlyTyr: 3.234 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	1.048HisAla: 1.048 ± 0.029	0.295HisCys: 0.295 ± 0.015	0.83HisAsp: 0.83 ± 0.026	1.101HisGlu: 1.101 ± 0.038	0.753HisPhe: 0.753 ± 0.026	1.303HisGly: 1.303 ± 0.036	0.403HisHis: 0.403 ± 0.024	1.419HisIle: 1.419 ± 0.039	0.916HisLys: 0.916 ± 0.03	1.535HisLeu: 1.535 ± 0.037	0.606HisMet: 0.606 ± 0.024	0.699HisAsn: 0.699 ± 0.026	0.888HisPro: 0.888 ± 0.028	0.576HisGln: 0.576 ± 0.021	0.95HisArg: 0.95 ± 0.029	1.022HisSer: 1.022 ± 0.025	0.986HisThr: 0.986 ± 0.028	1.068HisVal: 1.068 ± 0.03	0.163HisTrp: 0.163 ± 0.01	0.73HisTyr: 0.73 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.038IleAla: 5.038 ± 0.063	1.326IleCys: 1.326 ± 0.034	3.361IleAsp: 3.361 ± 0.06	4.384IleGlu: 4.384 ± 0.076	2.884IlePhe: 2.884 ± 0.056	4.452IleGly: 4.452 ± 0.071	1.321IleHis: 1.321 ± 0.036	4.632IleIle: 4.632 ± 0.083	3.587IleLys: 3.587 ± 0.065	7.294IleLeu: 7.294 ± 0.093	1.936IleMet: 1.936 ± 0.044	2.804IleAsn: 2.804 ± 0.052	3.0IlePro: 3.0 ± 0.056	2.538IleGln: 2.538 ± 0.042	4.12IleArg: 4.12 ± 0.068	5.247IleSer: 5.247 ± 0.069	4.169IleThr: 4.169 ± 0.061	4.261IleVal: 4.261 ± 0.066	0.621IleTrp: 0.621 ± 0.022	2.717IleTyr: 2.717 ± 0.057	0.0IleXaa: 0.0 ± 0.0
Lys	4.555LysAla: 4.555 ± 0.069	0.686LysCys: 0.686 ± 0.029	3.548LysAsp: 3.548 ± 0.067	6.622LysGlu: 6.622 ± 0.087	1.965LysPhe: 1.965 ± 0.042	4.107LysGly: 4.107 ± 0.064	0.992LysHis: 0.992 ± 0.026	4.897LysIle: 4.897 ± 0.069	5.891LysLys: 5.891 ± 0.094	5.222LysLeu: 5.222 ± 0.072	2.031LysMet: 2.031 ± 0.042	3.532LysAsn: 3.532 ± 0.069	1.972LysPro: 1.972 ± 0.043	2.615LysGln: 2.615 ± 0.05	3.054LysArg: 3.054 ± 0.059	3.533LysSer: 3.533 ± 0.061	3.644LysThr: 3.644 ± 0.065	3.989LysVal: 3.989 ± 0.069	0.59LysTrp: 0.59 ± 0.024	2.759LysTyr: 2.759 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	6.787LeuAla: 6.787 ± 0.091	1.472LeuCys: 1.472 ± 0.038	4.776LeuAsp: 4.776 ± 0.063	7.595LeuGlu: 7.595 ± 0.093	3.741LeuPhe: 3.741 ± 0.077	5.474LeuGly: 5.474 ± 0.079	1.463LeuHis: 1.463 ± 0.036	5.551LeuIle: 5.551 ± 0.088	6.47LeuLys: 6.47 ± 0.083	8.797LeuLeu: 8.797 ± 0.117	2.603LeuMet: 2.603 ± 0.052	3.918LeuAsn: 3.918 ± 0.064	3.499LeuPro: 3.499 ± 0.064	3.093LeuGln: 3.093 ± 0.051	3.897LeuArg: 3.897 ± 0.066	6.222LeuSer: 6.222 ± 0.079	4.91LeuThr: 4.91 ± 0.065	5.199LeuVal: 5.199 ± 0.074	0.805LeuTrp: 0.805 ± 0.031	3.373LeuTyr: 3.373 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	2.515MetAla: 2.515 ± 0.053	0.34MetCys: 0.34 ± 0.018	1.898MetAsp: 1.898 ± 0.041	3.403MetGlu: 3.403 ± 0.061	1.027MetPhe: 1.027 ± 0.033	2.211MetGly: 2.211 ± 0.047	0.406MetHis: 0.406 ± 0.019	1.978MetIle: 1.978 ± 0.046	2.601MetLys: 2.601 ± 0.045	2.654MetLeu: 2.654 ± 0.053	0.93MetMet: 0.93 ± 0.029	1.481MetAsn: 1.481 ± 0.031	1.124MetPro: 1.124 ± 0.031	1.126MetGln: 1.126 ± 0.032	1.289MetArg: 1.289 ± 0.037	1.656MetSer: 1.656 ± 0.039	1.634MetThr: 1.634 ± 0.042	2.047MetVal: 2.047 ± 0.049	0.186MetTrp: 0.186 ± 0.013	0.953MetTyr: 0.953 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	2.824AsnAla: 2.824 ± 0.054	0.665AsnCys: 0.665 ± 0.027	1.902AsnAsp: 1.902 ± 0.043	2.695AsnGlu: 2.695 ± 0.056	1.63AsnPhe: 1.63 ± 0.037	3.453AsnGly: 3.453 ± 0.059	0.885AsnHis: 0.885 ± 0.028	3.382AsnIle: 3.382 ± 0.058	2.179AsnLys: 2.179 ± 0.049	3.997AsnLeu: 3.997 ± 0.067	1.394AsnMet: 1.394 ± 0.032	1.809AsnAsn: 1.809 ± 0.048	1.97AsnPro: 1.97 ± 0.039	1.687AsnGln: 1.687 ± 0.042	2.573AsnArg: 2.573 ± 0.052	2.386AsnSer: 2.386 ± 0.05	2.342AsnThr: 2.342 ± 0.05	2.754AsnVal: 2.754 ± 0.044	0.375AsnTrp: 0.375 ± 0.019	1.829AsnTyr: 1.829 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	2.468ProAla: 2.468 ± 0.05	0.475ProCys: 0.475 ± 0.021	2.299ProAsp: 2.299 ± 0.045	4.042ProGlu: 4.042 ± 0.08	1.512ProPhe: 1.512 ± 0.036	2.603ProGly: 2.603 ± 0.052	0.521ProHis: 0.521 ± 0.022	1.666ProIle: 1.666 ± 0.042	1.739ProLys: 1.739 ± 0.036	2.629ProLeu: 2.629 ± 0.051	0.891ProMet: 0.891 ± 0.031	1.057ProAsn: 1.057 ± 0.031	0.836ProPro: 0.836 ± 0.03	1.119ProGln: 1.119 ± 0.034	1.093ProArg: 1.093 ± 0.033	1.523ProSer: 1.523 ± 0.036	1.214ProThr: 1.214 ± 0.031	3.22ProVal: 3.22 ± 0.055	0.342ProTrp: 0.342 ± 0.018	1.247ProTyr: 1.247 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	2.869GlnAla: 2.869 ± 0.049	0.401GlnCys: 0.401 ± 0.022	1.682GlnAsp: 1.682 ± 0.041	4.072GlnGlu: 4.072 ± 0.075	1.286GlnPhe: 1.286 ± 0.035	2.429GlnGly: 2.429 ± 0.051	0.49GlnHis: 0.49 ± 0.019	2.681GlnIle: 2.681 ± 0.05	3.006GlnLys: 3.006 ± 0.056	2.766GlnLeu: 2.766 ± 0.054	1.389GlnMet: 1.389 ± 0.039	1.827GlnAsn: 1.827 ± 0.037	1.021GlnPro: 1.021 ± 0.033	1.613GlnGln: 1.613 ± 0.043	1.503GlnArg: 1.503 ± 0.039	1.674GlnSer: 1.674 ± 0.04	1.836GlnThr: 1.836 ± 0.047	2.407GlnVal: 2.407 ± 0.042	0.32GlnTrp: 0.32 ± 0.017	1.407GlnTyr: 1.407 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.776ArgAla: 2.776 ± 0.039	0.619ArgCys: 0.619 ± 0.024	2.301ArgAsp: 2.301 ± 0.049	4.971ArgGlu: 4.971 ± 0.076	2.014ArgPhe: 2.014 ± 0.046	2.711ArgGly: 2.711 ± 0.053	0.844ArgHis: 0.844 ± 0.03	3.828ArgIle: 3.828 ± 0.062	4.415ArgLys: 4.415 ± 0.067	4.28ArgLeu: 4.28 ± 0.065	1.767ArgMet: 1.767 ± 0.039	2.568ArgAsn: 2.568 ± 0.05	1.472ArgPro: 1.472 ± 0.04	2.248ArgGln: 2.248 ± 0.05	2.749ArgArg: 2.749 ± 0.058	2.354ArgSer: 2.354 ± 0.046	2.464ArgThr: 2.464 ± 0.048	2.707ArgVal: 2.707 ± 0.055	0.462ArgTrp: 0.462 ± 0.02	2.067ArgTyr: 2.067 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	4.227SerAla: 4.227 ± 0.071	0.91SerCys: 0.91 ± 0.025	3.08SerAsp: 3.08 ± 0.052	4.408SerGlu: 4.408 ± 0.066	2.668SerPhe: 2.668 ± 0.053	5.415SerGly: 5.415 ± 0.086	1.103SerHis: 1.103 ± 0.034	3.773SerIle: 3.773 ± 0.063	2.711SerLys: 2.711 ± 0.052	5.474SerLeu: 5.474 ± 0.078	1.933SerMet: 1.933 ± 0.045	2.049SerAsn: 2.049 ± 0.048	1.884SerPro: 1.884 ± 0.039	2.16SerGln: 2.16 ± 0.045	3.302SerArg: 3.302 ± 0.052	3.681SerSer: 3.681 ± 0.069	2.51SerThr: 2.51 ± 0.054	4.463SerVal: 4.463 ± 0.063	0.611SerTrp: 0.611 ± 0.025	2.374SerTyr: 2.374 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	4.254ThrAla: 4.254 ± 0.077	0.698ThrCys: 0.698 ± 0.026	3.093ThrAsp: 3.093 ± 0.055	4.636ThrGlu: 4.636 ± 0.07	2.027ThrPhe: 2.027 ± 0.046	5.196ThrGly: 5.196 ± 0.093	0.83ThrHis: 0.83 ± 0.025	3.47ThrIle: 3.47 ± 0.059	2.484ThrLys: 2.484 ± 0.053	4.485ThrLeu: 4.485 ± 0.056	1.449ThrMet: 1.449 ± 0.039	1.724ThrAsn: 1.724 ± 0.042	1.975ThrPro: 1.975 ± 0.04	1.414ThrGln: 1.414 ± 0.033	2.294ThrArg: 2.294 ± 0.04	2.786ThrSer: 2.786 ± 0.048	2.378ThrThr: 2.378 ± 0.059	4.528ThrVal: 4.528 ± 0.078	0.477ThrTrp: 0.477 ± 0.02	1.902ThrTyr: 1.902 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	4.188ValAla: 4.188 ± 0.069	1.248ValCys: 1.248 ± 0.035	3.247ValAsp: 3.247 ± 0.054	4.874ValGlu: 4.874 ± 0.08	2.919ValPhe: 2.919 ± 0.048	3.784ValGly: 3.784 ± 0.067	1.044ValHis: 1.044 ± 0.027	4.904ValIle: 4.904 ± 0.068	4.457ValLys: 4.457 ± 0.063	6.588ValLeu: 6.588 ± 0.08	1.984ValMet: 1.984 ± 0.039	2.805ValAsn: 2.805 ± 0.048	2.446ValPro: 2.446 ± 0.051	2.201ValGln: 2.201 ± 0.046	3.287ValArg: 3.287 ± 0.055	4.893ValSer: 4.893 ± 0.068	4.054ValThr: 4.054 ± 0.072	4.493ValVal: 4.493 ± 0.075	0.659ValTrp: 0.659 ± 0.022	2.654ValTyr: 2.654 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.528TrpAla: 0.528 ± 0.023	0.158TrpCys: 0.158 ± 0.013	0.508TrpAsp: 0.508 ± 0.021	0.808TrpGlu: 0.808 ± 0.025	0.407TrpPhe: 0.407 ± 0.022	0.598TrpGly: 0.598 ± 0.025	0.181TrpHis: 0.181 ± 0.012	0.621TrpIle: 0.621 ± 0.023	0.838TrpLys: 0.838 ± 0.03	0.879TrpLeu: 0.879 ± 0.029	0.333TrpMet: 0.333 ± 0.018	0.566TrpAsn: 0.566 ± 0.025	0.187TrpPro: 0.187 ± 0.013	0.454TrpGln: 0.454 ± 0.022	0.361TrpArg: 0.361 ± 0.021	0.497TrpSer: 0.497 ± 0.024	0.396TrpThr: 0.396 ± 0.02	0.485TrpVal: 0.485 ± 0.023	0.096TrpTrp: 0.096 ± 0.01	0.398TrpTyr: 0.398 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.509TyrAla: 2.509 ± 0.048	0.67TyrCys: 0.67 ± 0.029	2.345TyrAsp: 2.345 ± 0.051	3.161TyrGlu: 3.161 ± 0.058	1.852TyrPhe: 1.852 ± 0.039	3.06TyrGly: 3.06 ± 0.053	0.92TyrHis: 0.92 ± 0.031	2.655TyrIle: 2.655 ± 0.057	2.023TyrLys: 2.023 ± 0.05	3.732TyrLeu: 3.732 ± 0.054	1.129TyrMet: 1.129 ± 0.029	1.727TyrAsn: 1.727 ± 0.041	1.433TyrPro: 1.433 ± 0.033	1.839TyrGln: 1.839 ± 0.04	2.459TyrArg: 2.459 ± 0.051	2.283TyrSer: 2.283 ± 0.039	2.184TyrThr: 2.184 ± 0.053	2.454TyrVal: 2.454 ± 0.04	0.368TyrTrp: 0.368 ± 0.019	1.925TyrTyr: 1.925 ± 0.056	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3773 proteins (1163219 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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