Thermoanaerobacterium phage THSA-485A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3VYV3|I3VYV3_9CAUD Uncharacterized protein OS=Thermoanaerobacterium phage THSA-485A OX=1126885 GN=Tsac_2845 PE=4 SV=1
MM1 pKa = 7.42TGYY4 pKa = 10.82LFQVDD9 pKa = 4.04TNGKK13 pKa = 9.22IIAGQAIEE21 pKa = 4.03NWVNGDD27 pKa = 3.63ANVWNAQEE35 pKa = 3.82RR36 pKa = 11.84SFYY39 pKa = 11.06NATTGQWTIYY49 pKa = 10.94DD50 pKa = 5.13PIQINAPATASINTAFNVAATLPTNSPDD78 pKa = 3.14TSVEE82 pKa = 3.76FNVIYY87 pKa = 10.43NGQAGTPIPVNVTNLQATQQFNFAQAGTYY116 pKa = 8.65TITVSSEE123 pKa = 3.7HH124 pKa = 6.7HH125 pKa = 6.14GSASAEE131 pKa = 4.2VTVSS135 pKa = 2.97

Molecular weight: 14.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3VYX8|I3VYX8_9CAUD Uncharacterized protein OS=Thermoanaerobacterium phage THSA-485A OX=1126885 GN=Tsac_2870 PE=4 SV=1
MM1 pKa = 7.69EE2 pKa = 6.11DD3 pKa = 2.66NWLALGIAILSPRR16 pKa = 11.84FVTPEE21 pKa = 3.6EE22 pKa = 4.35AFDD25 pKa = 4.12LLYY28 pKa = 10.3IEE30 pKa = 5.32QRR32 pKa = 11.84TTRR35 pKa = 11.84HH36 pKa = 5.54KK37 pKa = 9.88PAKK40 pKa = 9.8RR41 pKa = 11.84LNKK44 pKa = 9.32TINDD48 pKa = 3.66KK49 pKa = 11.07DD50 pKa = 3.96VEE52 pKa = 4.27DD53 pKa = 3.97MIKK56 pKa = 10.69LKK58 pKa = 10.84FEE60 pKa = 3.57QSYY63 pKa = 8.78TYY65 pKa = 10.99KK66 pKa = 10.56EE67 pKa = 3.49IAKK70 pKa = 9.9IYY72 pKa = 9.89GLSADD77 pKa = 3.57AVYY80 pKa = 10.9GRR82 pKa = 11.84IRR84 pKa = 11.84RR85 pKa = 11.84YY86 pKa = 8.83KK87 pKa = 9.75KK88 pKa = 10.33KK89 pKa = 10.48RR90 pKa = 11.84GGPDD94 pKa = 2.79GRR96 pKa = 11.84KK97 pKa = 9.32AA98 pKa = 3.21

Molecular weight: 11.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12847

38

892

225.4

25.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.967 ± 0.52

0.981 ± 0.134

5.924 ± 0.302

7.27 ± 0.489

3.573 ± 0.225

6.188 ± 0.358

1.417 ± 0.138

8.111 ± 0.265

8.095 ± 0.626

8.072 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.39 ± 0.139

5.97 ± 0.316

3.822 ± 0.246

4.717 ± 0.243

3.176 ± 0.218

5.846 ± 0.346

6.048 ± 0.512

6.064 ± 0.275

1.51 ± 0.134

3.861 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski