Nitrosospira lacus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosospira

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2855 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6SLW1|A0A1W6SLW1_9PROT Tetratricopeptide repeat protein OS=Nitrosospira lacus OX=1288494 GN=EBAPG3_002895 PE=3 SV=1
MM1 pKa = 7.95ALMITDD7 pKa = 3.73EE8 pKa = 5.22CINCDD13 pKa = 3.27VCEE16 pKa = 4.27PEE18 pKa = 4.91CPNDD22 pKa = 4.45AISQGEE28 pKa = 4.54EE29 pKa = 4.0IYY31 pKa = 11.08VIAADD36 pKa = 4.68LCTEE40 pKa = 4.78CIGHH44 pKa = 6.59HH45 pKa = 5.57EE46 pKa = 4.25VSQCVEE52 pKa = 4.13VCPVDD57 pKa = 5.57CIILNPDD64 pKa = 2.89IVEE67 pKa = 4.4TEE69 pKa = 3.96AQLHH73 pKa = 5.44EE74 pKa = 5.15KK75 pKa = 10.38YY76 pKa = 10.36LALGNVV82 pKa = 3.53

Molecular weight: 8.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6ST75|A0A1W6ST75_9PROT Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Nitrosospira lacus OX=1288494 GN=gatA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.3TTGGAAVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.98GRR39 pKa = 11.84VRR41 pKa = 11.84LGVV44 pKa = 3.2

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2855

0

2855

913826

22

3724

320.1

35.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.222 ± 0.055

0.938 ± 0.017

5.236 ± 0.03

5.921 ± 0.043

3.841 ± 0.029

7.844 ± 0.046

2.397 ± 0.024

5.948 ± 0.029

4.228 ± 0.043

10.481 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.597 ± 0.025

3.448 ± 0.029

4.826 ± 0.037

3.742 ± 0.03

6.385 ± 0.038

5.872 ± 0.038

5.209 ± 0.035

6.85 ± 0.035

1.303 ± 0.022

2.713 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski