Thermosulfuriphilus ammonigenes

Taxonomy: cellular organisms; Bacteria; Thermodesulfobacteria; Thermodesulfobacteria; Thermodesulfobacteriales; Thermodesulfobacteriaceae; Thermosulfuriphilus

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2168 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G7PW66|A0A6G7PW66_9BACT TldD/PmbA family protein OS=Thermosulfuriphilus ammonigenes OX=1936021 GN=G4V39_06325 PE=3 SV=1
MM1 pKa = 7.75KK2 pKa = 10.26IFFDD6 pKa = 4.12PDD8 pKa = 2.86IPEE11 pKa = 4.54NIRR14 pKa = 11.84DD15 pKa = 4.17EE16 pKa = 4.14IASLIKK22 pKa = 10.32EE23 pKa = 4.44QITSPCKK30 pKa = 10.15CGCDD34 pKa = 3.47EE35 pKa = 5.27IYY37 pKa = 11.41VSMTDD42 pKa = 3.38NILDD46 pKa = 3.92VKK48 pKa = 10.94CYY50 pKa = 10.73DD51 pKa = 3.54CGEE54 pKa = 4.4SFFEE58 pKa = 4.32VALEE62 pKa = 4.22TEE64 pKa = 4.48EE65 pKa = 4.37GG66 pKa = 3.54

Molecular weight: 7.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G7PXL2|A0A6G7PXL2_9BACT Dicarboxylate/amino acid:cation symporter OS=Thermosulfuriphilus ammonigenes OX=1936021 GN=G4V39_09170 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 9.6RR3 pKa = 11.84TFQPHH8 pKa = 4.39NLKK11 pKa = 10.41RR12 pKa = 11.84KK13 pKa = 7.03RR14 pKa = 11.84THH16 pKa = 6.05GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.32TRR25 pKa = 11.84GGRR28 pKa = 11.84RR29 pKa = 11.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2168

0

2168

710334

37

1741

327.6

36.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.509 ± 0.057

1.134 ± 0.029

4.419 ± 0.033

7.804 ± 0.06

4.277 ± 0.038

7.544 ± 0.049

1.925 ± 0.023

6.447 ± 0.041

5.326 ± 0.054

12.022 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.881 ± 0.024

2.465 ± 0.032

5.153 ± 0.042

3.238 ± 0.031

7.285 ± 0.055

5.243 ± 0.036

4.221 ± 0.031

6.97 ± 0.046

1.165 ± 0.021

2.973 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski