Amino acid dipepetide frequency for Thermosulfuriphilus ammonigenes

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.498AlaAla: 7.498 ± 0.129	1.071AlaCys: 1.071 ± 0.052	3.282AlaAsp: 3.282 ± 0.07	6.055AlaGlu: 6.055 ± 0.129	3.534AlaPhe: 3.534 ± 0.075	6.424AlaGly: 6.424 ± 0.109	1.556AlaHis: 1.556 ± 0.058	5.531AlaIle: 5.531 ± 0.094	4.161AlaLys: 4.161 ± 0.082	11.331AlaLeu: 11.331 ± 0.156	1.844AlaMet: 1.844 ± 0.057	1.83AlaAsn: 1.83 ± 0.053	3.21AlaPro: 3.21 ± 0.067	3.358AlaGln: 3.358 ± 0.068	6.919AlaArg: 6.919 ± 0.111	4.454AlaSer: 4.454 ± 0.078	3.746AlaThr: 3.746 ± 0.08	5.723AlaVal: 5.723 ± 0.106	0.985AlaTrp: 0.985 ± 0.039	2.575AlaTyr: 2.575 ± 0.066	0.0AlaXaa: 0.0 ± 0.0
Cys	0.724CysAla: 0.724 ± 0.033	0.184CysCys: 0.184 ± 0.018	0.379CysAsp: 0.379 ± 0.024	0.614CysGlu: 0.614 ± 0.03	0.486CysPhe: 0.486 ± 0.029	1.215CysGly: 1.215 ± 0.052	0.708CysHis: 0.708 ± 0.072	0.443CysIle: 0.443 ± 0.027	0.283CysLys: 0.283 ± 0.018	1.489CysLeu: 1.489 ± 0.055	0.135CysMet: 0.135 ± 0.015	0.251CysAsn: 0.251 ± 0.02	1.04CysPro: 1.04 ± 0.05	0.595CysGln: 0.595 ± 0.031	0.891CysArg: 0.891 ± 0.034	0.555CysSer: 0.555 ± 0.03	0.317CysThr: 0.317 ± 0.024	0.572CysVal: 0.572 ± 0.032	0.124CysTrp: 0.124 ± 0.015	0.339CysTyr: 0.339 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	2.15AspAla: 2.15 ± 0.056	0.534AspCys: 0.534 ± 0.032	1.808AspAsp: 1.808 ± 0.061	3.166AspGlu: 3.166 ± 0.071	2.503AspPhe: 2.503 ± 0.06	2.896AspGly: 2.896 ± 0.077	1.031AspHis: 1.031 ± 0.037	3.232AspIle: 3.232 ± 0.071	1.888AspLys: 1.888 ± 0.064	7.071AspLeu: 7.071 ± 0.1	0.776AspMet: 0.776 ± 0.037	1.005AspAsn: 1.005 ± 0.04	3.08AspPro: 3.08 ± 0.063	1.554AspGln: 1.554 ± 0.052	3.065AspArg: 3.065 ± 0.068	1.951AspSer: 1.951 ± 0.059	1.598AspThr: 1.598 ± 0.048	2.575AspVal: 2.575 ± 0.057	0.676AspTrp: 0.676 ± 0.033	1.633AspTyr: 1.633 ± 0.049	0.0AspXaa: 0.0 ± 0.0
Glu	9.018GluAla: 9.018 ± 0.142	0.48GluCys: 0.48 ± 0.024	3.76GluAsp: 3.76 ± 0.082	8.167GluGlu: 8.167 ± 0.126	2.307GluPhe: 2.307 ± 0.058	5.706GluGly: 5.706 ± 0.108	1.147GluHis: 1.147 ± 0.043	6.603GluIle: 6.603 ± 0.11	5.029GluLys: 5.029 ± 0.108	7.622GluLeu: 7.622 ± 0.126	1.691GluMet: 1.691 ± 0.043	1.974GluAsn: 1.974 ± 0.05	2.578GluPro: 2.578 ± 0.058	1.557GluGln: 1.557 ± 0.055	5.482GluArg: 5.482 ± 0.1	2.832GluSer: 2.832 ± 0.065	3.749GluThr: 3.749 ± 0.074	6.224GluVal: 6.224 ± 0.114	0.518GluTrp: 0.518 ± 0.031	1.598GluTyr: 1.598 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	2.761PheAla: 2.761 ± 0.078	0.652PheCys: 0.652 ± 0.032	2.074PheAsp: 2.074 ± 0.064	2.725PheGlu: 2.725 ± 0.069	2.431PhePhe: 2.431 ± 0.071	3.131PheGly: 3.131 ± 0.07	0.774PheHis: 0.774 ± 0.031	2.628PheIle: 2.628 ± 0.068	1.954PheLys: 1.954 ± 0.054	5.457PheLeu: 5.457 ± 0.108	0.881PheMet: 0.881 ± 0.042	1.166PheAsn: 1.166 ± 0.037	2.099PhePro: 2.099 ± 0.054	1.116PheGln: 1.116 ± 0.043	2.927PheArg: 2.927 ± 0.066	3.011PheSer: 3.011 ± 0.076	1.746PheThr: 1.746 ± 0.049	2.899PheVal: 2.899 ± 0.072	0.711PheTrp: 0.711 ± 0.034	1.625PheTyr: 1.625 ± 0.052	0.0PheXaa: 0.0 ± 0.0
Gly	5.862GlyAla: 5.862 ± 0.109	1.011GlyCys: 1.011 ± 0.043	3.13GlyAsp: 3.13 ± 0.072	5.207GlyGlu: 5.207 ± 0.084	3.262GlyPhe: 3.262 ± 0.073	5.55GlyGly: 5.55 ± 0.125	1.792GlyHis: 1.792 ± 0.058	4.699GlyIle: 4.699 ± 0.086	3.736GlyLys: 3.736 ± 0.081	10.219GlyLeu: 10.219 ± 0.138	1.394GlyMet: 1.394 ± 0.042	1.594GlyAsn: 1.594 ± 0.052	3.508GlyPro: 3.508 ± 0.077	3.135GlyGln: 3.135 ± 0.073	6.024GlyArg: 6.024 ± 0.093	3.428GlySer: 3.428 ± 0.077	3.134GlyThr: 3.134 ± 0.068	5.303GlyVal: 5.303 ± 0.087	0.866GlyTrp: 0.866 ± 0.042	2.585GlyTyr: 2.585 ± 0.076	0.0GlyXaa: 0.0 ± 0.0
His	1.0HisAla: 1.0 ± 0.039	0.258HisCys: 0.258 ± 0.019	0.715HisAsp: 0.715 ± 0.034	1.052HisGlu: 1.052 ± 0.042	1.029HisPhe: 1.029 ± 0.044	1.389HisGly: 1.389 ± 0.054	0.581HisHis: 0.581 ± 0.031	1.071HisIle: 1.071 ± 0.045	0.669HisLys: 0.669 ± 0.032	3.092HisLeu: 3.092 ± 0.062	0.332HisMet: 0.332 ± 0.022	0.535HisAsn: 0.535 ± 0.032	1.48HisPro: 1.48 ± 0.052	0.864HisGln: 0.864 ± 0.035	1.571HisArg: 1.571 ± 0.045	0.914HisSer: 0.914 ± 0.035	0.729HisThr: 0.729 ± 0.034	0.966HisVal: 0.966 ± 0.031	0.253HisTrp: 0.253 ± 0.02	0.752HisTyr: 0.752 ± 0.038	0.0HisXaa: 0.0 ± 0.0
Ile	4.415IleAla: 4.415 ± 0.087	0.825IleCys: 0.825 ± 0.04	3.344IleAsp: 3.344 ± 0.069	4.311IleGlu: 4.311 ± 0.084	3.162IlePhe: 3.162 ± 0.069	3.971IleGly: 3.971 ± 0.088	1.228IleHis: 1.228 ± 0.046	4.464IleIle: 4.464 ± 0.091	3.985IleLys: 3.985 ± 0.08	7.568IleLeu: 7.568 ± 0.12	1.135IleMet: 1.135 ± 0.04	2.106IleAsn: 2.106 ± 0.057	3.448IlePro: 3.448 ± 0.069	1.778IleGln: 1.778 ± 0.044	4.606IleArg: 4.606 ± 0.075	4.147IleSer: 4.147 ± 0.076	2.961IleThr: 2.961 ± 0.066	4.208IleVal: 4.208 ± 0.095	0.7IleTrp: 0.7 ± 0.031	2.106IleTyr: 2.106 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	5.02LysAla: 5.02 ± 0.098	0.367LysCys: 0.367 ± 0.026	2.952LysAsp: 2.952 ± 0.063	6.006LysGlu: 6.006 ± 0.11	1.337LysPhe: 1.337 ± 0.054	4.516LysGly: 4.516 ± 0.076	0.698LysHis: 0.698 ± 0.032	4.109LysIle: 4.109 ± 0.084	3.932LysLys: 3.932 ± 0.1	4.202LysLeu: 4.202 ± 0.084	1.223LysMet: 1.223 ± 0.04	1.499LysAsn: 1.499 ± 0.051	1.906LysPro: 1.906 ± 0.049	1.09LysGln: 1.09 ± 0.043	3.838LysArg: 3.838 ± 0.073	2.008LysSer: 2.008 ± 0.058	2.814LysThr: 2.814 ± 0.063	4.166LysVal: 4.166 ± 0.09	0.389LysTrp: 0.389 ± 0.025	1.191LysTyr: 1.191 ± 0.04	0.0LysXaa: 0.0 ± 0.0
Leu	12.267LeuAla: 12.267 ± 0.169	1.218LeuCys: 1.218 ± 0.043	5.233LeuAsp: 5.233 ± 0.085	9.75LeuGlu: 9.75 ± 0.143	4.971LeuPhe: 4.971 ± 0.094	8.969LeuGly: 8.969 ± 0.143	1.806LeuHis: 1.806 ± 0.055	6.922LeuIle: 6.922 ± 0.113	8.707LeuLys: 8.707 ± 0.115	12.556LeuLeu: 12.556 ± 0.183	2.054LeuMet: 2.054 ± 0.052	2.972LeuAsn: 2.972 ± 0.066	6.058LeuPro: 6.058 ± 0.084	2.723LeuGln: 2.723 ± 0.067	7.915LeuArg: 7.915 ± 0.11	8.189LeuSer: 8.189 ± 0.111	5.127LeuThr: 5.127 ± 0.084	8.207LeuVal: 8.207 ± 0.113	1.487LeuTrp: 1.487 ± 0.055	3.09LeuTyr: 3.09 ± 0.071	0.0LeuXaa: 0.0 ± 0.0
Met	2.642MetAla: 2.642 ± 0.064	0.166MetCys: 0.166 ± 0.016	0.89MetAsp: 0.89 ± 0.039	1.468MetGlu: 1.468 ± 0.045	0.532MetPhe: 0.532 ± 0.029	1.649MetGly: 1.649 ± 0.054	0.207MetHis: 0.207 ± 0.019	1.23MetIle: 1.23 ± 0.044	1.233MetLys: 1.233 ± 0.04	1.526MetLeu: 1.526 ± 0.047	0.438MetMet: 0.438 ± 0.03	0.536MetAsn: 0.536 ± 0.028	0.933MetPro: 0.933 ± 0.038	0.331MetGln: 0.331 ± 0.021	1.108MetArg: 1.108 ± 0.038	0.914MetSer: 0.914 ± 0.039	0.914MetThr: 0.914 ± 0.037	1.602MetVal: 1.602 ± 0.056	0.172MetTrp: 0.172 ± 0.017	0.32MetTyr: 0.32 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	1.553AsnAla: 1.553 ± 0.056	0.327AsnCys: 0.327 ± 0.029	0.943AsnAsp: 0.943 ± 0.042	1.237AsnGlu: 1.237 ± 0.044	1.212AsnPhe: 1.212 ± 0.044	1.339AsnGly: 1.339 ± 0.05	0.521AsnHis: 0.521 ± 0.028	1.879AsnIle: 1.879 ± 0.051	1.156AsnLys: 1.156 ± 0.048	3.688AsnLeu: 3.688 ± 0.08	0.463AsnMet: 0.463 ± 0.026	0.705AsnAsn: 0.705 ± 0.039	1.941AsnPro: 1.941 ± 0.059	1.099AsnGln: 1.099 ± 0.045	1.739AsnArg: 1.739 ± 0.054	1.043AsnSer: 1.043 ± 0.041	0.956AsnThr: 0.956 ± 0.037	1.622AsnVal: 1.622 ± 0.043	0.359AsnTrp: 0.359 ± 0.021	0.863AsnTyr: 0.863 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	3.576ProAla: 3.576 ± 0.074	0.473ProCys: 0.473 ± 0.026	2.572ProAsp: 2.572 ± 0.057	5.523ProGlu: 5.523 ± 0.078	2.321ProPhe: 2.321 ± 0.064	4.246ProGly: 4.246 ± 0.085	1.102ProHis: 1.102 ± 0.044	2.374ProIle: 2.374 ± 0.065	2.2ProLys: 2.2 ± 0.052	5.8ProLeu: 5.8 ± 0.1	0.777ProMet: 0.777 ± 0.032	1.06ProAsn: 1.06 ± 0.039	3.172ProPro: 3.172 ± 0.066	2.181ProGln: 2.181 ± 0.054	3.334ProArg: 3.334 ± 0.069	2.789ProSer: 2.789 ± 0.062	1.902ProThr: 1.902 ± 0.051	3.725ProVal: 3.725 ± 0.071	0.712ProTrp: 0.712 ± 0.033	1.689ProTyr: 1.689 ± 0.056	0.0ProXaa: 0.0 ± 0.0
Gln	3.684GlnAla: 3.684 ± 0.075	0.297GlnCys: 0.297 ± 0.022	1.384GlnAsp: 1.384 ± 0.048	3.159GlnGlu: 3.159 ± 0.069	0.84GlnPhe: 0.84 ± 0.04	2.88GlnGly: 2.88 ± 0.072	0.417GlnHis: 0.417 ± 0.022	2.283GlnIle: 2.283 ± 0.054	2.35GlnLys: 2.35 ± 0.061	2.633GlnLeu: 2.633 ± 0.064	0.764GlnMet: 0.764 ± 0.033	0.752GlnAsn: 0.752 ± 0.031	1.178GlnPro: 1.178 ± 0.039	0.876GlnGln: 0.876 ± 0.038	2.51GlnArg: 2.51 ± 0.067	1.277GlnSer: 1.277 ± 0.042	1.582GlnThr: 1.582 ± 0.057	2.602GlnVal: 2.602 ± 0.061	0.293GlnTrp: 0.293 ± 0.02	0.617GlnTyr: 0.617 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	5.834ArgAla: 5.834 ± 0.094	0.819ArgCys: 0.819 ± 0.044	2.9ArgAsp: 2.9 ± 0.062	6.26ArgGlu: 6.26 ± 0.112	3.144ArgPhe: 3.144 ± 0.073	4.879ArgGly: 4.879 ± 0.087	1.556ArgHis: 1.556 ± 0.044	4.409ArgIle: 4.409 ± 0.089	3.16ArgLys: 3.16 ± 0.076	10.547ArgLeu: 10.547 ± 0.147	1.233ArgMet: 1.233 ± 0.043	1.482ArgAsn: 1.482 ± 0.052	4.054ArgPro: 4.054 ± 0.074	2.989ArgGln: 2.989 ± 0.082	6.287ArgArg: 6.287 ± 0.123	3.075ArgSer: 3.075 ± 0.062	2.26ArgThr: 2.26 ± 0.064	4.647ArgVal: 4.647 ± 0.092	0.871ArgTrp: 0.871 ± 0.038	2.44ArgTyr: 2.44 ± 0.056	0.0ArgXaa: 0.0 ± 0.0
Ser	3.552SerAla: 3.552 ± 0.068	0.68SerCys: 0.68 ± 0.038	1.961SerAsp: 1.961 ± 0.057	3.238SerGlu: 3.238 ± 0.075	2.617SerPhe: 2.617 ± 0.071	4.705SerGly: 4.705 ± 0.098	1.054SerHis: 1.054 ± 0.04	2.54SerIle: 2.54 ± 0.065	1.919SerLys: 1.919 ± 0.063	7.675SerLeu: 7.675 ± 0.12	0.963SerMet: 0.963 ± 0.037	1.088SerAsn: 1.088 ± 0.039	3.764SerPro: 3.764 ± 0.066	2.306SerGln: 2.306 ± 0.065	4.105SerArg: 4.105 ± 0.073	3.027SerSer: 3.027 ± 0.079	1.929SerThr: 1.929 ± 0.054	2.993SerVal: 2.993 ± 0.06	0.791SerTrp: 0.791 ± 0.034	1.526SerTyr: 1.526 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	3.515ThrAla: 3.515 ± 0.067	0.538ThrCys: 0.538 ± 0.031	1.775ThrAsp: 1.775 ± 0.049	2.51ThrGlu: 2.51 ± 0.07	1.882ThrPhe: 1.882 ± 0.052	3.988ThrGly: 3.988 ± 0.089	0.753ThrHis: 0.753 ± 0.036	2.985ThrIle: 2.985 ± 0.064	1.95ThrLys: 1.95 ± 0.054	4.803ThrLeu: 4.803 ± 0.09	0.857ThrMet: 0.857 ± 0.033	1.195ThrAsn: 1.195 ± 0.04	2.431ThrPro: 2.431 ± 0.065	1.099ThrGln: 1.099 ± 0.039	2.413ThrArg: 2.413 ± 0.056	2.316ThrSer: 2.316 ± 0.06	2.164ThrThr: 2.164 ± 0.062	3.125ThrVal: 3.125 ± 0.072	0.465ThrTrp: 0.465 ± 0.025	1.443ThrTyr: 1.443 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	7.098ValAla: 7.098 ± 0.098	0.97ValCys: 0.97 ± 0.044	3.265ValAsp: 3.265 ± 0.065	4.833ValGlu: 4.833 ± 0.094	3.291ValPhe: 3.291 ± 0.074	4.779ValGly: 4.779 ± 0.081	1.294ValHis: 1.294 ± 0.043	4.909ValIle: 4.909 ± 0.086	3.46ValLys: 3.46 ± 0.08	7.161ValLeu: 7.161 ± 0.109	1.206ValMet: 1.206 ± 0.044	1.83ValAsn: 1.83 ± 0.051	3.248ValPro: 3.248 ± 0.073	1.612ValGln: 1.612 ± 0.044	4.66ValArg: 4.66 ± 0.092	4.473ValSer: 4.473 ± 0.08	3.076ValThr: 3.076 ± 0.06	5.989ValVal: 5.989 ± 0.115	0.662ValTrp: 0.662 ± 0.032	1.882ValTyr: 1.882 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	1.081TrpAla: 1.081 ± 0.042	0.118TrpCys: 0.118 ± 0.012	0.481TrpAsp: 0.481 ± 0.025	0.85TrpGlu: 0.85 ± 0.035	0.504TrpPhe: 0.504 ± 0.028	0.97TrpGly: 0.97 ± 0.039	0.246TrpHis: 0.246 ± 0.019	0.629TrpIle: 0.629 ± 0.033	0.429TrpLys: 0.429 ± 0.026	1.436TrpLeu: 1.436 ± 0.048	0.218TrpMet: 0.218 ± 0.018	0.279TrpAsn: 0.279 ± 0.018	0.712TrpPro: 0.712 ± 0.032	0.552TrpGln: 0.552 ± 0.03	0.962TrpArg: 0.962 ± 0.037	0.541TrpSer: 0.541 ± 0.027	0.404TrpThr: 0.404 ± 0.024	0.715TrpVal: 0.715 ± 0.028	0.198TrpTrp: 0.198 ± 0.018	0.325TrpTyr: 0.325 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.837TyrAla: 1.837 ± 0.057	0.356TyrCys: 0.356 ± 0.025	1.344TyrAsp: 1.344 ± 0.042	1.909TyrGlu: 1.909 ± 0.057	1.401TyrPhe: 1.401 ± 0.045	2.237TyrGly: 2.237 ± 0.061	0.78TyrHis: 0.78 ± 0.032	1.525TyrIle: 1.525 ± 0.052	1.061TyrLys: 1.061 ± 0.04	4.343TyrLeu: 4.343 ± 0.081	0.424TyrMet: 0.424 ± 0.027	0.787TyrAsn: 0.787 ± 0.04	1.687TyrPro: 1.687 ± 0.047	1.563TyrGln: 1.563 ± 0.04	2.493TyrArg: 2.493 ± 0.064	1.491TyrSer: 1.491 ± 0.054	1.102TyrThr: 1.102 ± 0.037	1.837TyrVal: 1.837 ± 0.056	0.421TyrTrp: 0.421 ± 0.027	1.129TyrTyr: 1.129 ± 0.051	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2168 proteins (710334 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---