Pelagibacter sp. (strain IMCC9063)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; Pelagibacteraceae; Candidatus Pelagibacter; unclassified Candidatus Pelagibacter

Average proteome isoelectric point is 7.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1447 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2I0V2|F2I0V2_PELSM Inosine-5'-monophosphate dehydrogenase OS=Pelagibacter sp. (strain IMCC9063) OX=1002672 GN=guaB PE=3 SV=1
MM1 pKa = 7.63PKK3 pKa = 8.85VTYY6 pKa = 10.2IEE8 pKa = 4.44FNGTEE13 pKa = 3.79HH14 pKa = 5.9VVNVEE19 pKa = 3.73KK20 pKa = 10.84GLTIMEE26 pKa = 4.17GAVQNNIPGIDD37 pKa = 3.7ADD39 pKa = 4.42CGGSCACATCHH50 pKa = 6.79VYY52 pKa = 10.7VDD54 pKa = 4.76DD55 pKa = 4.82DD56 pKa = 4.03WVDD59 pKa = 3.67KK60 pKa = 11.29LPEE63 pKa = 3.83QSDD66 pKa = 3.96AEE68 pKa = 4.23KK69 pKa = 11.39DD70 pKa = 3.39MLDD73 pKa = 3.53FAFEE77 pKa = 4.35TKK79 pKa = 8.91STSRR83 pKa = 11.84LSCQLFLDD91 pKa = 4.22EE92 pKa = 5.7DD93 pKa = 4.47LDD95 pKa = 4.9GIAVSLPEE103 pKa = 4.04KK104 pKa = 10.62QGG106 pKa = 3.27

Molecular weight: 11.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2I3Z6|F2I3Z6_PELSM Uncharacterized protein OS=Pelagibacter sp. (strain IMCC9063) OX=1002672 GN=SAR11G3_00559 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.33KK10 pKa = 9.63VRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.46GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.49SGVKK29 pKa = 9.86VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.61GRR39 pKa = 11.84KK40 pKa = 9.11RR41 pKa = 11.84ISAA44 pKa = 3.72

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1447

0

1447

404044

37

1520

279.2

31.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.74 ± 0.067

1.012 ± 0.021

4.903 ± 0.055

5.784 ± 0.07

5.584 ± 0.073

6.154 ± 0.065

1.648 ± 0.027

9.369 ± 0.069

10.403 ± 0.12

9.564 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.162 ± 0.031

6.19 ± 0.074

3.193 ± 0.035

2.893 ± 0.034

3.174 ± 0.044

7.316 ± 0.054

4.9 ± 0.043

5.783 ± 0.054

0.89 ± 0.023

3.339 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski