Amino acid dipepetide frequency for Pelagibacter sp. (strain IMCC9063)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.351AlaAla: 4.351 ± 0.153
0.673AlaCys: 0.673 ± 0.045
2.74AlaAsp: 2.74 ± 0.089
3.166AlaGlu: 3.166 ± 0.121
2.75AlaPhe: 2.75 ± 0.083
4.203AlaGly: 4.203 ± 0.123
0.943AlaHis: 0.943 ± 0.052
5.329AlaIle: 5.329 ± 0.132
5.457AlaLys: 5.457 ± 0.144
5.457AlaLeu: 5.457 ± 0.144
1.512AlaMet: 1.512 ± 0.069
2.893AlaAsn: 2.893 ± 0.084
1.629AlaPro: 1.629 ± 0.071
1.589AlaGln: 1.589 ± 0.062
1.96AlaArg: 1.96 ± 0.07
4.059AlaSer: 4.059 ± 0.112
2.871AlaThr: 2.871 ± 0.082
3.485AlaVal: 3.485 ± 0.111
0.53AlaTrp: 0.53 ± 0.037
1.807AlaTyr: 1.807 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
0.614CysAla: 0.614 ± 0.048
0.158CysCys: 0.158 ± 0.022
0.559CysAsp: 0.559 ± 0.04
0.544CysGlu: 0.544 ± 0.036
0.606CysPhe: 0.606 ± 0.042
0.839CysGly: 0.839 ± 0.047
0.213CysHis: 0.213 ± 0.023
0.881CysIle: 0.881 ± 0.044
0.898CysLys: 0.898 ± 0.043
0.918CysLeu: 0.918 ± 0.051
0.183CysMet: 0.183 ± 0.02
0.535CysAsn: 0.535 ± 0.035
0.379CysPro: 0.379 ± 0.031
0.235CysGln: 0.235 ± 0.026
0.275CysArg: 0.275 ± 0.026
0.799CysSer: 0.799 ± 0.046
0.47CysThr: 0.47 ± 0.039
0.621CysVal: 0.621 ± 0.041
0.079CysTrp: 0.079 ± 0.015
0.314CysTyr: 0.314 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
2.463AspAla: 2.463 ± 0.08
0.52AspCys: 0.52 ± 0.036
2.136AspAsp: 2.136 ± 0.08
3.121AspGlu: 3.121 ± 0.094
2.925AspPhe: 2.925 ± 0.095
2.935AspGly: 2.935 ± 0.099
0.995AspHis: 0.995 ± 0.059
4.47AspIle: 4.47 ± 0.114
4.579AspLys: 4.579 ± 0.129
5.227AspLeu: 5.227 ± 0.115
0.965AspMet: 0.965 ± 0.052
2.591AspAsn: 2.591 ± 0.073
1.814AspPro: 1.814 ± 0.066
1.747AspGln: 1.747 ± 0.057
1.718AspArg: 1.718 ± 0.06
3.131AspSer: 3.131 ± 0.094
2.227AspThr: 2.227 ± 0.089
2.98AspVal: 2.98 ± 0.097
0.604AspTrp: 0.604 ± 0.041
1.879AspTyr: 1.879 ± 0.079
0.0AspXaa: 0.0 ± 0.0
Glu
3.19GluAla: 3.19 ± 0.097
0.49GluCys: 0.49 ± 0.036
2.901GluAsp: 2.901 ± 0.084
4.034GluGlu: 4.034 ± 0.134
2.505GluPhe: 2.505 ± 0.077
2.95GluGly: 2.95 ± 0.099
0.95GluHis: 0.95 ± 0.051
6.19GluIle: 6.19 ± 0.13
6.89GluLys: 6.89 ± 0.164
5.108GluLeu: 5.108 ± 0.136
1.359GluMet: 1.359 ± 0.059
3.901GluAsn: 3.901 ± 0.11
1.445GluPro: 1.445 ± 0.067
1.965GluGln: 1.965 ± 0.075
2.034GluArg: 2.034 ± 0.071
3.282GluSer: 3.282 ± 0.094
2.789GluThr: 2.789 ± 0.084
3.574GluVal: 3.574 ± 0.101
0.54GluTrp: 0.54 ± 0.04
1.737GluTyr: 1.737 ± 0.058
0.0GluXaa: 0.0 ± 0.0
Phe
3.079PheAla: 3.079 ± 0.099
0.676PheCys: 0.676 ± 0.039
2.856PheAsp: 2.856 ± 0.082
2.871PheGlu: 2.871 ± 0.089
4.16PhePhe: 4.16 ± 0.149
3.626PheGly: 3.626 ± 0.105
0.841PheHis: 0.841 ± 0.05
4.631PheIle: 4.631 ± 0.129
4.841PheLys: 4.841 ± 0.125
6.338PheLeu: 6.338 ± 0.18
0.943PheMet: 0.943 ± 0.054
3.23PheAsn: 3.23 ± 0.103
1.507PhePro: 1.507 ± 0.061
1.505PheGln: 1.505 ± 0.06
1.544PheArg: 1.544 ± 0.055
4.537PheSer: 4.537 ± 0.135
2.517PheThr: 2.517 ± 0.072
3.304PheVal: 3.304 ± 0.109
0.562PheTrp: 0.562 ± 0.037
2.267PheTyr: 2.267 ± 0.093
0.0PheXaa: 0.0 ± 0.0
Gly
3.876GlyAla: 3.876 ± 0.116
0.752GlyCys: 0.752 ± 0.043
2.866GlyAsp: 2.866 ± 0.082
2.99GlyGlu: 2.99 ± 0.088
3.47GlyPhe: 3.47 ± 0.091
4.487GlyGly: 4.487 ± 0.141
1.161GlyHis: 1.161 ± 0.056
5.636GlyIle: 5.636 ± 0.147
5.44GlyLys: 5.44 ± 0.11
5.712GlyLeu: 5.712 ± 0.145
1.614GlyMet: 1.614 ± 0.076
2.777GlyAsn: 2.777 ± 0.086
1.794GlyPro: 1.794 ± 0.067
1.668GlyGln: 1.668 ± 0.067
2.052GlyArg: 2.052 ± 0.073
4.537GlySer: 4.537 ± 0.108
3.638GlyThr: 3.638 ± 0.104
4.012GlyVal: 4.012 ± 0.108
0.725GlyTrp: 0.725 ± 0.042
2.329GlyTyr: 2.329 ± 0.077
0.0GlyXaa: 0.0 ± 0.0
His
0.985HisAla: 0.985 ± 0.051
0.25HisCys: 0.25 ± 0.023
0.851HisAsp: 0.851 ± 0.053
0.866HisGlu: 0.866 ± 0.053
0.874HisPhe: 0.874 ± 0.047
1.203HisGly: 1.203 ± 0.05
0.374HisHis: 0.374 ± 0.033
1.277HisIle: 1.277 ± 0.061
1.406HisLys: 1.406 ± 0.075
1.527HisLeu: 1.527 ± 0.052
0.376HisMet: 0.376 ± 0.028
0.874HisAsn: 0.874 ± 0.047
0.864HisPro: 0.864 ± 0.048
0.634HisGln: 0.634 ± 0.038
0.609HisArg: 0.609 ± 0.043
1.091HisSer: 1.091 ± 0.049
0.757HisThr: 0.757 ± 0.047
0.854HisVal: 0.854 ± 0.046
0.178HisTrp: 0.178 ± 0.024
0.626HisTyr: 0.626 ± 0.044
0.0HisXaa: 0.0 ± 0.0
Ile
5.668IleAla: 5.668 ± 0.141
1.069IleCys: 1.069 ± 0.057
4.915IleAsp: 4.915 ± 0.121
5.529IleGlu: 5.529 ± 0.102
5.769IlePhe: 5.769 ± 0.159
5.519IleGly: 5.519 ± 0.138
1.416IleHis: 1.416 ± 0.061
8.039IleIle: 8.039 ± 0.182
9.546IleLys: 9.546 ± 0.193
9.049IleLeu: 9.049 ± 0.167
1.72IleMet: 1.72 ± 0.073
5.841IleAsn: 5.841 ± 0.155
3.22IlePro: 3.22 ± 0.094
2.666IleGln: 2.666 ± 0.086
2.653IleArg: 2.653 ± 0.086
7.4IleSer: 7.4 ± 0.17
4.482IleThr: 4.482 ± 0.11
5.472IleVal: 5.472 ± 0.12
0.634IleTrp: 0.634 ± 0.037
3.084IleTyr: 3.084 ± 0.093
0.0IleXaa: 0.0 ± 0.0
Lys
4.336LysAla: 4.336 ± 0.127
0.643LysCys: 0.643 ± 0.038
5.348LysAsp: 5.348 ± 0.103
6.868LysGlu: 6.868 ± 0.143
4.876LysPhe: 4.876 ± 0.124
4.443LysGly: 4.443 ± 0.106
1.403LysHis: 1.403 ± 0.062
11.662LysIle: 11.662 ± 0.229
15.595LysLys: 15.595 ± 0.367
8.19LysLeu: 8.19 ± 0.162
2.183LysMet: 2.183 ± 0.069
9.581LysAsn: 9.581 ± 0.193
2.609LysPro: 2.609 ± 0.085
2.713LysGln: 2.713 ± 0.097
3.027LysArg: 3.027 ± 0.089
6.252LysSer: 6.252 ± 0.138
4.975LysThr: 4.975 ± 0.12
5.18LysVal: 5.18 ± 0.127
0.646LysTrp: 0.646 ± 0.042
3.505LysTyr: 3.505 ± 0.101
0.0LysXaa: 0.0 ± 0.0
Leu
5.947LeuAla: 5.947 ± 0.142
0.869LeuCys: 0.869 ± 0.052
4.745LeuAsp: 4.745 ± 0.121
5.722LeuGlu: 5.722 ± 0.135
5.16LeuPhe: 5.16 ± 0.138
5.987LeuGly: 5.987 ± 0.14
1.393LeuHis: 1.393 ± 0.058
8.818LeuIle: 8.818 ± 0.191
9.796LeuLys: 9.796 ± 0.183
8.702LeuLeu: 8.702 ± 0.194
2.168LeuMet: 2.168 ± 0.075
6.044LeuAsn: 6.044 ± 0.147
3.272LeuPro: 3.272 ± 0.114
2.544LeuGln: 2.544 ± 0.077
3.133LeuArg: 3.133 ± 0.1
7.794LeuSer: 7.794 ± 0.16
4.819LeuThr: 4.819 ± 0.115
5.507LeuVal: 5.507 ± 0.139
0.72LeuTrp: 0.72 ± 0.045
2.495LeuTyr: 2.495 ± 0.086
0.0LeuXaa: 0.0 ± 0.0
Met
1.453MetAla: 1.453 ± 0.06
0.21MetCys: 0.21 ± 0.023
1.03MetAsp: 1.03 ± 0.048
1.074MetGlu: 1.074 ± 0.057
0.886MetPhe: 0.886 ± 0.056
1.619MetGly: 1.619 ± 0.077
0.381MetHis: 0.381 ± 0.037
1.834MetIle: 1.834 ± 0.074
2.126MetLys: 2.126 ± 0.076
1.908MetLeu: 1.908 ± 0.071
0.53MetMet: 0.53 ± 0.04
1.416MetAsn: 1.416 ± 0.058
0.988MetPro: 0.988 ± 0.05
0.646MetGln: 0.646 ± 0.041
0.767MetArg: 0.767 ± 0.047
1.671MetSer: 1.671 ± 0.065
1.138MetThr: 1.138 ± 0.054
1.213MetVal: 1.213 ± 0.059
0.181MetTrp: 0.181 ± 0.019
0.547MetTyr: 0.547 ± 0.039
0.0MetXaa: 0.0 ± 0.0
Asn
2.774AsnAla: 2.774 ± 0.084
0.641AsnCys: 0.641 ± 0.043
2.542AsnAsp: 2.542 ± 0.077
3.19AsnGlu: 3.19 ± 0.11
4.284AsnPhe: 4.284 ± 0.125
2.651AsnGly: 2.651 ± 0.097
1.012AsnHis: 1.012 ± 0.05
6.46AsnIle: 6.46 ± 0.158
7.071AsnLys: 7.071 ± 0.174
6.673AsnLeu: 6.673 ± 0.151
1.233AsnMet: 1.233 ± 0.057
4.24AsnAsn: 4.24 ± 0.128
2.331AsnPro: 2.331 ± 0.075
1.973AsnGln: 1.973 ± 0.075
1.631AsnArg: 1.631 ± 0.068
4.267AsnSer: 4.267 ± 0.11
2.784AsnThr: 2.784 ± 0.084
3.047AsnVal: 3.047 ± 0.087
0.53AsnTrp: 0.53 ± 0.042
2.572AsnTyr: 2.572 ± 0.089
0.0AsnXaa: 0.0 ± 0.0
Pro
1.75ProAla: 1.75 ± 0.086
0.309ProCys: 0.309 ± 0.026
1.802ProAsp: 1.802 ± 0.072
2.223ProGlu: 2.223 ± 0.08
1.631ProPhe: 1.631 ± 0.07
2.24ProGly: 2.24 ± 0.079
0.592ProHis: 0.592 ± 0.036
2.953ProIle: 2.953 ± 0.081
2.836ProLys: 2.836 ± 0.091
2.866ProLeu: 2.866 ± 0.091
0.648ProMet: 0.648 ± 0.04
1.812ProAsn: 1.812 ± 0.069
0.859ProPro: 0.859 ± 0.049
0.844ProGln: 0.844 ± 0.042
1.02ProArg: 1.02 ± 0.047
2.453ProSer: 2.453 ± 0.083
1.713ProThr: 1.713 ± 0.067
2.005ProVal: 2.005 ± 0.079
0.364ProTrp: 0.364 ± 0.032
1.017ProTyr: 1.017 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
1.685GlnAla: 1.685 ± 0.074
0.223GlnCys: 0.223 ± 0.021
1.431GlnAsp: 1.431 ± 0.058
1.97GlnGlu: 1.97 ± 0.08
1.384GlnPhe: 1.384 ± 0.05
1.683GlnGly: 1.683 ± 0.075
0.45GlnHis: 0.45 ± 0.038
2.906GlnIle: 2.906 ± 0.073
3.252GlnLys: 3.252 ± 0.085
2.666GlnLeu: 2.666 ± 0.082
0.629GlnMet: 0.629 ± 0.037
1.864GlnAsn: 1.864 ± 0.078
0.822GlnPro: 0.822 ± 0.046
0.839GlnGln: 0.839 ± 0.047
1.02GlnArg: 1.02 ± 0.052
2.101GlnSer: 2.101 ± 0.084
1.317GlnThr: 1.317 ± 0.056
1.586GlnVal: 1.586 ± 0.061
0.243GlnTrp: 0.243 ± 0.028
0.856GlnTyr: 0.856 ± 0.049
0.0GlnXaa: 0.0 ± 0.0
Arg
1.943ArgAla: 1.943 ± 0.084
0.299ArgCys: 0.299 ± 0.025
1.539ArgAsp: 1.539 ± 0.061
1.911ArgGlu: 1.911 ± 0.072
1.671ArgPhe: 1.671 ± 0.07
2.015ArgGly: 2.015 ± 0.074
0.505ArgHis: 0.505 ± 0.036
2.784ArgIle: 2.784 ± 0.085
3.024ArgLys: 3.024 ± 0.093
2.888ArgLeu: 2.888 ± 0.084
0.725ArgMet: 0.725 ± 0.039
1.869ArgAsn: 1.869 ± 0.067
1.079ArgPro: 1.079 ± 0.059
0.948ArgGln: 0.948 ± 0.044
1.213ArgArg: 1.213 ± 0.055
2.403ArgSer: 2.403 ± 0.068
1.542ArgThr: 1.542 ± 0.069
2.057ArgVal: 2.057 ± 0.07
0.299ArgTrp: 0.299 ± 0.031
1.03ArgTyr: 1.03 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
3.977SerAla: 3.977 ± 0.108
0.686SerCys: 0.686 ± 0.042
3.272SerAsp: 3.272 ± 0.09
3.898SerGlu: 3.898 ± 0.111
4.43SerPhe: 4.43 ± 0.125
4.829SerGly: 4.829 ± 0.114
1.188SerHis: 1.188 ± 0.052
6.979SerIle: 6.979 ± 0.165
7.814SerLys: 7.814 ± 0.145
7.373SerLeu: 7.373 ± 0.137
1.673SerMet: 1.673 ± 0.066
4.284SerAsn: 4.284 ± 0.121
1.893SerPro: 1.893 ± 0.072
2.086SerGln: 2.086 ± 0.076
2.262SerArg: 2.262 ± 0.079
5.888SerSer: 5.888 ± 0.141
3.341SerThr: 3.341 ± 0.084
4.002SerVal: 4.002 ± 0.097
0.624SerTrp: 0.624 ± 0.043
2.658SerTyr: 2.658 ± 0.082
0.0SerXaa: 0.0 ± 0.0
Thr
3.069ThrAla: 3.069 ± 0.108
0.52ThrCys: 0.52 ± 0.042
2.354ThrAsp: 2.354 ± 0.083
2.329ThrGlu: 2.329 ± 0.072
2.586ThrPhe: 2.586 ± 0.074
3.495ThrGly: 3.495 ± 0.096
0.881ThrHis: 0.881 ± 0.043
4.715ThrIle: 4.715 ± 0.124
4.641ThrLys: 4.641 ± 0.132
4.65ThrLeu: 4.65 ± 0.112
0.98ThrMet: 0.98 ± 0.05
2.839ThrAsn: 2.839 ± 0.089
1.933ThrPro: 1.933 ± 0.078
1.25ThrGln: 1.25 ± 0.05
1.624ThrArg: 1.624 ± 0.072
3.661ThrSer: 3.661 ± 0.103
2.675ThrThr: 2.675 ± 0.091
2.883ThrVal: 2.883 ± 0.104
0.374ThrTrp: 0.374 ± 0.032
1.544ThrTyr: 1.544 ± 0.055
0.0ThrXaa: 0.0 ± 0.0
Val
3.925ValAla: 3.925 ± 0.121
0.639ValCys: 0.639 ± 0.04
2.982ValAsp: 2.982 ± 0.084
3.193ValGlu: 3.193 ± 0.107
3.104ValPhe: 3.104 ± 0.108
4.118ValGly: 4.118 ± 0.114
0.98ValHis: 0.98 ± 0.048
4.967ValIle: 4.967 ± 0.119
4.878ValLys: 4.878 ± 0.115
5.576ValLeu: 5.576 ± 0.133
1.369ValMet: 1.369 ± 0.059
3.017ValAsn: 3.017 ± 0.076
2.002ValPro: 2.002 ± 0.074
1.577ValGln: 1.577 ± 0.063
1.735ValArg: 1.735 ± 0.077
4.428ValSer: 4.428 ± 0.118
3.19ValThr: 3.19 ± 0.104
3.841ValVal: 3.841 ± 0.124
0.599ValTrp: 0.599 ± 0.039
1.705ValTyr: 1.705 ± 0.076
0.0ValXaa: 0.0 ± 0.0
Trp
0.49TrpAla: 0.49 ± 0.032
0.121TrpCys: 0.121 ± 0.016
0.478TrpAsp: 0.478 ± 0.037
0.391TrpGlu: 0.391 ± 0.033
0.53TrpPhe: 0.53 ± 0.035
0.592TrpGly: 0.592 ± 0.04
0.225TrpHis: 0.225 ± 0.024
0.77TrpIle: 0.77 ± 0.048
0.834TrpLys: 0.834 ± 0.046
0.832TrpLeu: 0.832 ± 0.057
0.223TrpMet: 0.223 ± 0.023
0.512TrpAsn: 0.512 ± 0.034
0.29TrpPro: 0.29 ± 0.024
0.252TrpGln: 0.252 ± 0.029
0.401TrpArg: 0.401 ± 0.035
0.718TrpSer: 0.718 ± 0.046
0.389TrpThr: 0.389 ± 0.032
0.488TrpVal: 0.488 ± 0.033
0.104TrpTrp: 0.104 ± 0.015
0.267TrpTyr: 0.267 ± 0.03
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.827TyrAla: 1.827 ± 0.068
0.374TyrCys: 0.374 ± 0.031
1.681TyrAsp: 1.681 ± 0.065
1.945TyrGlu: 1.945 ± 0.076
2.237TyrPhe: 2.237 ± 0.082
2.104TyrGly: 2.104 ± 0.074
0.572TyrHis: 0.572 ± 0.037
2.391TyrIle: 2.391 ± 0.07
3.109TyrLys: 3.109 ± 0.107
3.975TyrLeu: 3.975 ± 0.125
0.582TyrMet: 0.582 ± 0.038
1.787TyrAsn: 1.787 ± 0.069
1.205TyrPro: 1.205 ± 0.055
1.245TyrGln: 1.245 ± 0.062
1.067TyrArg: 1.067 ± 0.044
2.688TyrSer: 2.688 ± 0.085
1.366TyrThr: 1.366 ± 0.061
1.715TyrVal: 1.715 ± 0.06
0.371TyrTrp: 0.371 ± 0.03
1.151TyrTyr: 1.151 ± 0.06
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1447 proteins (404044 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski