Amino acid dipepetide frequency for Lactococcus fujiensis JCM 16395

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.851AlaAla: 5.851 ± 0.133
0.417AlaCys: 0.417 ± 0.024
4.052AlaAsp: 4.052 ± 0.082
4.696AlaGlu: 4.696 ± 0.101
3.336AlaPhe: 3.336 ± 0.083
5.297AlaGly: 5.297 ± 0.108
1.382AlaHis: 1.382 ± 0.052
5.858AlaIle: 5.858 ± 0.109
5.041AlaLys: 5.041 ± 0.126
7.278AlaLeu: 7.278 ± 0.133
2.022AlaMet: 2.022 ± 0.071
3.501AlaAsn: 3.501 ± 0.104
2.18AlaPro: 2.18 ± 0.064
3.188AlaGln: 3.188 ± 0.104
2.75AlaArg: 2.75 ± 0.07
4.619AlaSer: 4.619 ± 0.095
4.647AlaThr: 4.647 ± 0.106
4.986AlaVal: 4.986 ± 0.092
0.693AlaTrp: 0.693 ± 0.037
2.518AlaTyr: 2.518 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
0.3CysAla: 0.3 ± 0.026
0.05CysCys: 0.05 ± 0.012
0.264CysAsp: 0.264 ± 0.024
0.306CysGlu: 0.306 ± 0.026
0.271CysPhe: 0.271 ± 0.024
0.503CysGly: 0.503 ± 0.033
0.128CysHis: 0.128 ± 0.015
0.278CysIle: 0.278 ± 0.022
0.189CysLys: 0.189 ± 0.019
0.456CysLeu: 0.456 ± 0.028
0.106CysMet: 0.106 ± 0.014
0.18CysAsn: 0.18 ± 0.017
0.189CysPro: 0.189 ± 0.018
0.195CysGln: 0.195 ± 0.02
0.145CysArg: 0.145 ± 0.017
0.313CysSer: 0.313 ± 0.024
0.214CysThr: 0.214 ± 0.019
0.244CysVal: 0.244 ± 0.019
0.049CysTrp: 0.049 ± 0.01
0.162CysTyr: 0.162 ± 0.018
0.002CysXaa: 0.002 ± 0.002
Asp
3.543AspAla: 3.543 ± 0.073
0.273AspCys: 0.273 ± 0.019
2.814AspAsp: 2.814 ± 0.079
4.764AspGlu: 4.764 ± 0.111
3.297AspPhe: 3.297 ± 0.078
3.742AspGly: 3.742 ± 0.092
1.02AspHis: 1.02 ± 0.046
3.646AspIle: 3.646 ± 0.082
3.794AspLys: 3.794 ± 0.087
5.644AspLeu: 5.644 ± 0.109
1.257AspMet: 1.257 ± 0.044
2.54AspAsn: 2.54 ± 0.069
1.633AspPro: 1.633 ± 0.049
1.959AspGln: 1.959 ± 0.065
1.907AspArg: 1.907 ± 0.061
3.0AspSer: 3.0 ± 0.08
2.681AspThr: 2.681 ± 0.076
3.641AspVal: 3.641 ± 0.084
0.682AspTrp: 0.682 ± 0.034
2.468AspTyr: 2.468 ± 0.068
0.0AspXaa: 0.0 ± 0.0
Glu
4.577GluAla: 4.577 ± 0.114
0.227GluCys: 0.227 ± 0.023
3.122GluAsp: 3.122 ± 0.079
4.632GluGlu: 4.632 ± 0.12
2.92GluPhe: 2.92 ± 0.091
3.367GluGly: 3.367 ± 0.081
1.288GluHis: 1.288 ± 0.043
6.041GluIle: 6.041 ± 0.117
6.169GluLys: 6.169 ± 0.129
6.745GluLeu: 6.745 ± 0.141
2.028GluMet: 2.028 ± 0.062
4.572GluAsn: 4.572 ± 0.103
1.599GluPro: 1.599 ± 0.058
2.629GluGln: 2.629 ± 0.078
2.737GluArg: 2.737 ± 0.079
3.393GluSer: 3.393 ± 0.088
3.498GluThr: 3.498 ± 0.085
4.407GluVal: 4.407 ± 0.091
0.55GluTrp: 0.55 ± 0.034
1.948GluTyr: 1.948 ± 0.063
0.0GluXaa: 0.0 ± 0.0
Phe
3.646PheAla: 3.646 ± 0.075
0.239PheCys: 0.239 ± 0.021
3.415PheAsp: 3.415 ± 0.088
3.356PheGlu: 3.356 ± 0.081
2.368PhePhe: 2.368 ± 0.076
3.56PheGly: 3.56 ± 0.088
0.847PheHis: 0.847 ± 0.04
3.664PheIle: 3.664 ± 0.088
2.718PheLys: 2.718 ± 0.067
4.336PheLeu: 4.336 ± 0.108
1.219PheMet: 1.219 ± 0.05
2.456PheAsn: 2.456 ± 0.069
1.621PhePro: 1.621 ± 0.052
1.515PheGln: 1.515 ± 0.053
1.463PheArg: 1.463 ± 0.053
3.493PheSer: 3.493 ± 0.09
2.697PheThr: 2.697 ± 0.077
3.579PheVal: 3.579 ± 0.086
0.577PheTrp: 0.577 ± 0.032
1.897PheTyr: 1.897 ± 0.059
0.0PheXaa: 0.0 ± 0.0
Gly
4.807GlyAla: 4.807 ± 0.099
0.316GlyCys: 0.316 ± 0.024
3.227GlyAsp: 3.227 ± 0.083
3.774GlyGlu: 3.774 ± 0.082
3.567GlyPhe: 3.567 ± 0.084
4.282GlyGly: 4.282 ± 0.116
1.278GlyHis: 1.278 ± 0.038
5.56GlyIle: 5.56 ± 0.094
4.737GlyLys: 4.737 ± 0.103
6.711GlyLeu: 6.711 ± 0.12
1.845GlyMet: 1.845 ± 0.057
3.058GlyAsn: 3.058 ± 0.083
1.387GlyPro: 1.387 ± 0.057
2.769GlyGln: 2.769 ± 0.077
2.548GlyArg: 2.548 ± 0.066
3.969GlySer: 3.969 ± 0.1
4.307GlyThr: 4.307 ± 0.134
4.56GlyVal: 4.56 ± 0.091
0.791GlyTrp: 0.791 ± 0.04
2.58GlyTyr: 2.58 ± 0.07
0.0GlyXaa: 0.0 ± 0.0
His
1.264HisAla: 1.264 ± 0.047
0.123HisCys: 0.123 ± 0.014
1.062HisAsp: 1.062 ± 0.046
1.254HisGlu: 1.254 ± 0.048
1.158HisPhe: 1.158 ± 0.042
1.215HisGly: 1.215 ± 0.047
0.539HisHis: 0.539 ± 0.03
1.296HisIle: 1.296 ± 0.05
0.907HisLys: 0.907 ± 0.036
2.04HisLeu: 2.04 ± 0.061
0.385HisMet: 0.385 ± 0.025
0.683HisAsn: 0.683 ± 0.036
0.858HisPro: 0.858 ± 0.038
0.89HisGln: 0.89 ± 0.041
0.771HisArg: 0.771 ± 0.042
0.998HisSer: 0.998 ± 0.046
0.936HisThr: 0.936 ± 0.041
1.224HisVal: 1.224 ± 0.045
0.224HisTrp: 0.224 ± 0.019
0.828HisTyr: 0.828 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
6.08IleAla: 6.08 ± 0.118
0.481IleCys: 0.481 ± 0.033
4.546IleAsp: 4.546 ± 0.083
5.39IleGlu: 5.39 ± 0.13
3.759IlePhe: 3.759 ± 0.091
5.304IleGly: 5.304 ± 0.114
1.343IleHis: 1.343 ± 0.045
5.965IleIle: 5.965 ± 0.12
5.053IleLys: 5.053 ± 0.105
7.512IleLeu: 7.512 ± 0.157
1.867IleMet: 1.867 ± 0.062
3.957IleAsn: 3.957 ± 0.081
3.003IlePro: 3.003 ± 0.077
2.617IleGln: 2.617 ± 0.073
2.673IleArg: 2.673 ± 0.08
5.588IleSer: 5.588 ± 0.114
4.445IleThr: 4.445 ± 0.107
5.225IleVal: 5.225 ± 0.102
0.65IleTrp: 0.65 ± 0.036
2.671IleTyr: 2.671 ± 0.07
0.0IleXaa: 0.0 ± 0.0
Lys
5.317LysAla: 5.317 ± 0.109
0.209LysCys: 0.209 ± 0.018
3.308LysAsp: 3.308 ± 0.084
4.782LysGlu: 4.782 ± 0.095
2.853LysPhe: 2.853 ± 0.08
3.701LysGly: 3.701 ± 0.088
1.156LysHis: 1.156 ± 0.045
6.083LysIle: 6.083 ± 0.112
6.114LysLys: 6.114 ± 0.114
5.954LysLeu: 5.954 ± 0.107
2.636LysMet: 2.636 ± 0.073
4.494LysAsn: 4.494 ± 0.086
1.924LysPro: 1.924 ± 0.052
2.397LysGln: 2.397 ± 0.062
2.873LysArg: 2.873 ± 0.075
4.156LysSer: 4.156 ± 0.097
4.279LysThr: 4.279 ± 0.085
4.543LysVal: 4.543 ± 0.095
0.655LysTrp: 0.655 ± 0.032
2.365LysTyr: 2.365 ± 0.065
0.0LysXaa: 0.0 ± 0.0
Leu
7.755LeuAla: 7.755 ± 0.13
0.399LeuCys: 0.399 ± 0.03
5.395LeuAsp: 5.395 ± 0.107
5.885LeuGlu: 5.885 ± 0.129
4.614LeuPhe: 4.614 ± 0.108
6.241LeuGly: 6.241 ± 0.113
1.549LeuHis: 1.549 ± 0.046
7.751LeuIle: 7.751 ± 0.137
6.358LeuLys: 6.358 ± 0.104
9.196LeuLeu: 9.196 ± 0.2
2.56LeuMet: 2.56 ± 0.072
4.93LeuAsn: 4.93 ± 0.089
3.794LeuPro: 3.794 ± 0.081
3.264LeuGln: 3.264 ± 0.081
3.447LeuArg: 3.447 ± 0.086
7.341LeuSer: 7.341 ± 0.118
6.3LeuThr: 6.3 ± 0.103
6.129LeuVal: 6.129 ± 0.095
0.794LeuTrp: 0.794 ± 0.038
2.824LeuTyr: 2.824 ± 0.08
0.002LeuXaa: 0.002 ± 0.001
Met
1.998MetAla: 1.998 ± 0.053
0.093MetCys: 0.093 ± 0.013
1.372MetAsp: 1.372 ± 0.049
1.498MetGlu: 1.498 ± 0.048
0.953MetPhe: 0.953 ± 0.042
1.742MetGly: 1.742 ± 0.059
0.433MetHis: 0.433 ± 0.029
2.097MetIle: 2.097 ± 0.067
2.251MetLys: 2.251 ± 0.059
2.277MetLeu: 2.277 ± 0.064
0.875MetMet: 0.875 ± 0.04
1.624MetAsn: 1.624 ± 0.054
1.015MetPro: 1.015 ± 0.044
0.857MetGln: 0.857 ± 0.043
1.047MetArg: 1.047 ± 0.04
1.85MetSer: 1.85 ± 0.059
2.116MetThr: 2.116 ± 0.062
1.675MetVal: 1.675 ± 0.058
0.183MetTrp: 0.183 ± 0.015
0.65MetTyr: 0.65 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.644AsnAla: 3.644 ± 0.091
0.258AsnCys: 0.258 ± 0.022
2.697AsnAsp: 2.697 ± 0.07
3.464AsnGlu: 3.464 ± 0.1
2.649AsnPhe: 2.649 ± 0.068
3.821AsnGly: 3.821 ± 0.103
1.136AsnHis: 1.136 ± 0.049
3.725AsnIle: 3.725 ± 0.087
3.318AsnLys: 3.318 ± 0.083
5.099AsnLeu: 5.099 ± 0.094
1.355AsnMet: 1.355 ± 0.048
2.469AsnAsn: 2.469 ± 0.09
2.37AsnPro: 2.37 ± 0.061
2.577AsnGln: 2.577 ± 0.072
1.912AsnArg: 1.912 ± 0.054
3.353AsnSer: 3.353 ± 0.103
2.545AsnThr: 2.545 ± 0.068
3.336AsnVal: 3.336 ± 0.075
0.672AsnTrp: 0.672 ± 0.037
2.017AsnTyr: 2.017 ± 0.066
0.0AsnXaa: 0.0 ± 0.0
Pro
2.267ProAla: 2.267 ± 0.073
0.126ProCys: 0.126 ± 0.015
1.872ProAsp: 1.872 ± 0.055
2.824ProGlu: 2.824 ± 0.064
1.783ProPhe: 1.783 ± 0.057
1.715ProGly: 1.715 ± 0.056
0.658ProHis: 0.658 ± 0.037
2.456ProIle: 2.456 ± 0.07
2.212ProLys: 2.212 ± 0.068
3.154ProLeu: 3.154 ± 0.082
0.789ProMet: 0.789 ± 0.031
1.747ProAsn: 1.747 ± 0.05
0.579ProPro: 0.579 ± 0.03
1.192ProGln: 1.192 ± 0.045
0.995ProArg: 0.995 ± 0.036
2.158ProSer: 2.158 ± 0.073
2.163ProThr: 2.163 ± 0.078
2.338ProVal: 2.338 ± 0.065
0.34ProTrp: 0.34 ± 0.027
1.276ProTyr: 1.276 ± 0.049
0.0ProXaa: 0.0 ± 0.0
Gln
3.126GlnAla: 3.126 ± 0.075
0.116GlnCys: 0.116 ± 0.014
1.678GlnAsp: 1.678 ± 0.054
2.452GlnGlu: 2.452 ± 0.072
1.803GlnPhe: 1.803 ± 0.06
2.143GlnGly: 2.143 ± 0.065
0.611GlnHis: 0.611 ± 0.036
3.299GlnIle: 3.299 ± 0.083
3.026GlnLys: 3.026 ± 0.078
3.73GlnLeu: 3.73 ± 0.089
1.145GlnMet: 1.145 ± 0.042
2.267GlnAsn: 2.267 ± 0.062
1.113GlnPro: 1.113 ± 0.045
1.326GlnGln: 1.326 ± 0.043
1.333GlnArg: 1.333 ± 0.043
2.187GlnSer: 2.187 ± 0.067
2.309GlnThr: 2.309 ± 0.062
2.596GlnVal: 2.596 ± 0.063
0.348GlnTrp: 0.348 ± 0.027
1.256GlnTyr: 1.256 ± 0.048
0.0GlnXaa: 0.0 ± 0.0
Arg
2.543ArgAla: 2.543 ± 0.07
0.125ArgCys: 0.125 ± 0.014
2.05ArgAsp: 2.05 ± 0.066
2.759ArgGlu: 2.759 ± 0.079
1.843ArgPhe: 1.843 ± 0.061
2.217ArgGly: 2.217 ± 0.069
0.781ArgHis: 0.781 ± 0.035
2.86ArgIle: 2.86 ± 0.068
2.818ArgLys: 2.818 ± 0.085
3.71ArgLeu: 3.71 ± 0.087
1.015ArgMet: 1.015 ± 0.045
1.811ArgAsn: 1.811 ± 0.056
1.222ArgPro: 1.222 ± 0.049
1.542ArgGln: 1.542 ± 0.058
1.771ArgArg: 1.771 ± 0.056
1.946ArgSer: 1.946 ± 0.063
1.818ArgThr: 1.818 ± 0.064
2.405ArgVal: 2.405 ± 0.068
0.291ArgTrp: 0.291 ± 0.023
1.382ArgTyr: 1.382 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
4.503SerAla: 4.503 ± 0.097
0.274SerCys: 0.274 ± 0.023
3.686SerAsp: 3.686 ± 0.075
4.163SerGlu: 4.163 ± 0.084
3.064SerPhe: 3.064 ± 0.079
5.063SerGly: 5.063 ± 0.122
1.197SerHis: 1.197 ± 0.043
4.838SerIle: 4.838 ± 0.097
4.311SerLys: 4.311 ± 0.092
6.043SerLeu: 6.043 ± 0.12
1.422SerMet: 1.422 ± 0.043
3.427SerAsn: 3.427 ± 0.088
1.959SerPro: 1.959 ± 0.059
2.547SerGln: 2.547 ± 0.07
2.249SerArg: 2.249 ± 0.069
4.836SerSer: 4.836 ± 0.161
4.033SerThr: 4.033 ± 0.11
4.173SerVal: 4.173 ± 0.08
0.636SerTrp: 0.636 ± 0.034
2.314SerTyr: 2.314 ± 0.07
0.0SerXaa: 0.0 ± 0.0
Thr
4.501ThrAla: 4.501 ± 0.11
0.249ThrCys: 0.249 ± 0.023
3.429ThrAsp: 3.429 ± 0.072
3.343ThrGlu: 3.343 ± 0.07
2.92ThrPhe: 2.92 ± 0.077
4.602ThrGly: 4.602 ± 0.113
1.131ThrHis: 1.131 ± 0.042
4.659ThrIle: 4.659 ± 0.101
3.686ThrLys: 3.686 ± 0.083
5.43ThrLeu: 5.43 ± 0.099
1.299ThrMet: 1.299 ± 0.044
2.951ThrAsn: 2.951 ± 0.079
2.264ThrPro: 2.264 ± 0.073
1.974ThrGln: 1.974 ± 0.057
1.889ThrArg: 1.889 ± 0.052
4.095ThrSer: 4.095 ± 0.106
3.851ThrThr: 3.851 ± 0.13
4.336ThrVal: 4.336 ± 0.11
0.598ThrTrp: 0.598 ± 0.034
2.262ThrTyr: 2.262 ± 0.084
0.0ThrXaa: 0.0 ± 0.0
Val
5.498ValAla: 5.498 ± 0.118
0.325ValCys: 0.325 ± 0.025
3.661ValAsp: 3.661 ± 0.088
4.301ValGlu: 4.301 ± 0.1
2.924ValPhe: 2.924 ± 0.088
4.607ValGly: 4.607 ± 0.097
1.146ValHis: 1.146 ± 0.042
5.142ValIle: 5.142 ± 0.104
4.56ValLys: 4.56 ± 0.095
6.422ValLeu: 6.422 ± 0.12
1.761ValMet: 1.761 ± 0.053
3.4ValAsn: 3.4 ± 0.09
2.331ValPro: 2.331 ± 0.06
2.176ValGln: 2.176 ± 0.06
2.434ValArg: 2.434 ± 0.072
4.516ValSer: 4.516 ± 0.092
4.134ValThr: 4.134 ± 0.081
4.631ValVal: 4.631 ± 0.103
0.518ValTrp: 0.518 ± 0.03
2.121ValTyr: 2.121 ± 0.066
0.0ValXaa: 0.0 ± 0.0
Trp
0.603TrpAla: 0.603 ± 0.034
0.061TrpCys: 0.061 ± 0.01
0.53TrpAsp: 0.53 ± 0.031
0.544TrpGlu: 0.544 ± 0.034
0.492TrpPhe: 0.492 ± 0.033
0.655TrpGly: 0.655 ± 0.034
0.2TrpHis: 0.2 ± 0.02
0.707TrpIle: 0.707 ± 0.037
0.635TrpLys: 0.635 ± 0.04
1.124TrpLeu: 1.124 ± 0.048
0.258TrpMet: 0.258 ± 0.024
0.596TrpAsn: 0.596 ± 0.037
0.221TrpPro: 0.221 ± 0.02
0.5TrpGln: 0.5 ± 0.029
0.404TrpArg: 0.404 ± 0.026
0.665TrpSer: 0.665 ± 0.038
0.581TrpThr: 0.581 ± 0.038
0.529TrpVal: 0.529 ± 0.03
0.133TrpTrp: 0.133 ± 0.014
0.375TrpTyr: 0.375 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.491TyrAla: 2.491 ± 0.069
0.182TyrCys: 0.182 ± 0.018
2.124TyrAsp: 2.124 ± 0.063
2.262TyrGlu: 2.262 ± 0.065
2.092TyrPhe: 2.092 ± 0.056
2.294TyrGly: 2.294 ± 0.058
0.835TyrHis: 0.835 ± 0.042
2.205TyrIle: 2.205 ± 0.068
1.905TyrLys: 1.905 ± 0.061
3.666TyrLeu: 3.666 ± 0.088
0.757TyrMet: 0.757 ± 0.035
1.751TyrAsn: 1.751 ± 0.061
1.384TyrPro: 1.384 ± 0.05
1.749TyrGln: 1.749 ± 0.06
1.56TyrArg: 1.56 ± 0.061
2.262TyrSer: 2.262 ± 0.058
1.916TyrThr: 1.916 ± 0.068
2.054TyrVal: 2.054 ± 0.067
0.424TyrTrp: 0.424 ± 0.027
1.547TyrTyr: 1.547 ± 0.055
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.002XaaGly: 0.002 ± 0.002
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.002XaaMet: 0.002 ± 0.001
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2024 proteins (594089 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski