Amino acid dipepetide frequency for Gracilibacillus kekensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.492AlaAla: 4.492 ± 0.081
0.561AlaCys: 0.561 ± 0.023
3.361AlaAsp: 3.361 ± 0.059
4.49AlaGlu: 4.49 ± 0.074
3.047AlaPhe: 3.047 ± 0.054
4.566AlaGly: 4.566 ± 0.071
1.121AlaHis: 1.121 ± 0.033
6.058AlaIle: 6.058 ± 0.083
4.331AlaLys: 4.331 ± 0.074
6.412AlaLeu: 6.412 ± 0.089
1.8AlaMet: 1.8 ± 0.044
3.015AlaAsn: 3.015 ± 0.051
1.834AlaPro: 1.834 ± 0.041
2.102AlaGln: 2.102 ± 0.045
2.181AlaArg: 2.181 ± 0.044
3.967AlaSer: 3.967 ± 0.062
3.661AlaThr: 3.661 ± 0.062
4.618AlaVal: 4.618 ± 0.079
0.615AlaTrp: 0.615 ± 0.026
2.253AlaTyr: 2.253 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
0.331CysAla: 0.331 ± 0.017
0.06CysCys: 0.06 ± 0.008
0.347CysAsp: 0.347 ± 0.02
0.376CysGlu: 0.376 ± 0.018
0.289CysPhe: 0.289 ± 0.017
0.555CysGly: 0.555 ± 0.024
0.174CysHis: 0.174 ± 0.014
0.481CysIle: 0.481 ± 0.021
0.304CysLys: 0.304 ± 0.018
0.591CysLeu: 0.591 ± 0.024
0.135CysMet: 0.135 ± 0.012
0.31CysAsn: 0.31 ± 0.018
0.275CysPro: 0.275 ± 0.015
0.232CysGln: 0.232 ± 0.014
0.229CysArg: 0.229 ± 0.013
0.389CysSer: 0.389 ± 0.02
0.303CysThr: 0.303 ± 0.018
0.321CysVal: 0.321 ± 0.015
0.064CysTrp: 0.064 ± 0.008
0.238CysTyr: 0.238 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.295AspAla: 3.295 ± 0.062
0.301AspCys: 0.301 ± 0.017
2.972AspAsp: 2.972 ± 0.061
4.449AspGlu: 4.449 ± 0.078
2.6AspPhe: 2.6 ± 0.048
3.625AspGly: 3.625 ± 0.075
1.341AspHis: 1.341 ± 0.042
4.677AspIle: 4.677 ± 0.067
3.527AspLys: 3.527 ± 0.062
5.058AspLeu: 5.058 ± 0.068
1.4AspMet: 1.4 ± 0.034
2.524AspAsn: 2.524 ± 0.054
2.184AspPro: 2.184 ± 0.04
2.936AspGln: 2.936 ± 0.06
2.368AspArg: 2.368 ± 0.047
2.861AspSer: 2.861 ± 0.059
2.816AspThr: 2.816 ± 0.049
3.8AspVal: 3.8 ± 0.056
0.776AspTrp: 0.776 ± 0.029
2.396AspTyr: 2.396 ± 0.043
0.0AspXaa: 0.0 ± 0.0
Glu
5.27GluAla: 5.27 ± 0.072
0.315GluCys: 0.315 ± 0.018
4.482GluAsp: 4.482 ± 0.081
7.808GluGlu: 7.808 ± 0.119
2.437GluPhe: 2.437 ± 0.051
4.203GluGly: 4.203 ± 0.072
1.505GluHis: 1.505 ± 0.032
5.91GluIle: 5.91 ± 0.079
6.518GluLys: 6.518 ± 0.104
6.783GluLeu: 6.783 ± 0.088
2.381GluMet: 2.381 ± 0.05
4.345GluAsn: 4.345 ± 0.072
2.032GluPro: 2.032 ± 0.049
3.836GluGln: 3.836 ± 0.066
3.059GluArg: 3.059 ± 0.054
3.713GluSer: 3.713 ± 0.067
4.116GluThr: 4.116 ± 0.069
5.289GluVal: 5.289 ± 0.081
0.972GluTrp: 0.972 ± 0.029
2.387GluTyr: 2.387 ± 0.059
0.0GluXaa: 0.0 ± 0.0
Phe
2.852PheAla: 2.852 ± 0.062
0.301PheCys: 0.301 ± 0.018
2.59PheAsp: 2.59 ± 0.054
2.967PheGlu: 2.967 ± 0.047
2.404PhePhe: 2.404 ± 0.063
3.05PheGly: 3.05 ± 0.062
1.066PheHis: 1.066 ± 0.031
4.105PheIle: 4.105 ± 0.077
2.282PheLys: 2.282 ± 0.049
4.669PheLeu: 4.669 ± 0.085
1.145PheMet: 1.145 ± 0.031
1.948PheAsn: 1.948 ± 0.047
1.61PhePro: 1.61 ± 0.04
1.844PheGln: 1.844 ± 0.046
1.628PheArg: 1.628 ± 0.037
3.273PheSer: 3.273 ± 0.057
2.655PheThr: 2.655 ± 0.059
3.132PheVal: 3.132 ± 0.06
0.515PheTrp: 0.515 ± 0.022
1.839PheTyr: 1.839 ± 0.04
0.0PheXaa: 0.0 ± 0.0
Gly
4.193GlyAla: 4.193 ± 0.075
0.496GlyCys: 0.496 ± 0.021
3.289GlyAsp: 3.289 ± 0.06
4.518GlyGlu: 4.518 ± 0.073
3.228GlyPhe: 3.228 ± 0.062
4.266GlyGly: 4.266 ± 0.087
1.162GlyHis: 1.162 ± 0.031
5.537GlyIle: 5.537 ± 0.077
4.325GlyLys: 4.325 ± 0.069
6.063GlyLeu: 6.063 ± 0.083
1.878GlyMet: 1.878 ± 0.043
2.916GlyAsn: 2.916 ± 0.063
1.576GlyPro: 1.576 ± 0.042
2.05GlyGln: 2.05 ± 0.044
2.17GlyArg: 2.17 ± 0.049
3.807GlySer: 3.807 ± 0.061
3.716GlyThr: 3.716 ± 0.074
4.718GlyVal: 4.718 ± 0.068
0.778GlyTrp: 0.778 ± 0.029
2.765GlyTyr: 2.765 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
1.293HisAla: 1.293 ± 0.034
0.176HisCys: 0.176 ± 0.013
1.127HisAsp: 1.127 ± 0.034
1.257HisGlu: 1.257 ± 0.036
1.193HisPhe: 1.193 ± 0.033
1.285HisGly: 1.285 ± 0.035
0.706HisHis: 0.706 ± 0.027
1.64HisIle: 1.64 ± 0.042
1.027HisLys: 1.027 ± 0.03
2.209HisLeu: 2.209 ± 0.047
0.468HisMet: 0.468 ± 0.021
0.875HisAsn: 0.875 ± 0.024
1.133HisPro: 1.133 ± 0.038
1.139HisGln: 1.139 ± 0.035
0.839HisArg: 0.839 ± 0.025
1.156HisSer: 1.156 ± 0.028
1.074HisThr: 1.074 ± 0.028
1.367HisVal: 1.367 ± 0.034
0.232HisTrp: 0.232 ± 0.013
0.994HisTyr: 0.994 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.052IleAla: 6.052 ± 0.089
0.605IleCys: 0.605 ± 0.026
5.007IleAsp: 5.007 ± 0.071
6.239IleGlu: 6.239 ± 0.084
3.699IlePhe: 3.699 ± 0.077
5.991IleGly: 5.991 ± 0.08
1.831IleHis: 1.831 ± 0.044
6.752IleIle: 6.752 ± 0.096
4.834IleLys: 4.834 ± 0.076
7.363IleLeu: 7.363 ± 0.106
1.85IleMet: 1.85 ± 0.042
3.826IleAsn: 3.826 ± 0.063
3.402IlePro: 3.402 ± 0.062
3.138IleGln: 3.138 ± 0.048
2.957IleArg: 2.957 ± 0.054
5.353IleSer: 5.353 ± 0.08
4.859IleThr: 4.859 ± 0.061
5.579IleVal: 5.579 ± 0.081
0.769IleTrp: 0.769 ± 0.03
2.766IleTyr: 2.766 ± 0.044
0.0IleXaa: 0.0 ± 0.0
Lys
4.219LysAla: 4.219 ± 0.061
0.28LysCys: 0.28 ± 0.019
3.91LysAsp: 3.91 ± 0.066
6.703LysGlu: 6.703 ± 0.09
1.815LysPhe: 1.815 ± 0.04
4.036LysGly: 4.036 ± 0.067
1.426LysHis: 1.426 ± 0.04
4.627LysIle: 4.627 ± 0.066
5.735LysLys: 5.735 ± 0.088
5.463LysLeu: 5.463 ± 0.078
2.069LysMet: 2.069 ± 0.044
3.425LysAsn: 3.425 ± 0.063
1.979LysPro: 1.979 ± 0.043
3.522LysGln: 3.522 ± 0.068
2.998LysArg: 2.998 ± 0.054
3.471LysSer: 3.471 ± 0.053
3.319LysThr: 3.319 ± 0.056
4.517LysVal: 4.517 ± 0.062
0.93LysTrp: 0.93 ± 0.027
2.22LysTyr: 2.22 ± 0.041
0.0LysXaa: 0.0 ± 0.0
Leu
6.749LeuAla: 6.749 ± 0.08
0.534LeuCys: 0.534 ± 0.021
5.205LeuAsp: 5.205 ± 0.068
6.927LeuGlu: 6.927 ± 0.081
4.911LeuPhe: 4.911 ± 0.084
5.512LeuGly: 5.512 ± 0.076
1.91LeuHis: 1.91 ± 0.044
7.728LeuIle: 7.728 ± 0.122
6.099LeuLys: 6.099 ± 0.093
9.89LeuLeu: 9.89 ± 0.147
2.398LeuMet: 2.398 ± 0.048
4.328LeuAsn: 4.328 ± 0.062
3.782LeuPro: 3.782 ± 0.059
3.723LeuGln: 3.723 ± 0.056
3.232LeuArg: 3.232 ± 0.056
6.57LeuSer: 6.57 ± 0.085
5.59LeuThr: 5.59 ± 0.073
5.943LeuVal: 5.943 ± 0.073
0.895LeuTrp: 0.895 ± 0.027
3.118LeuTyr: 3.118 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
1.74MetAla: 1.74 ± 0.038
0.114MetCys: 0.114 ± 0.01
1.532MetAsp: 1.532 ± 0.039
2.15MetGlu: 2.15 ± 0.046
1.107MetPhe: 1.107 ± 0.036
1.47MetGly: 1.47 ± 0.036
0.457MetHis: 0.457 ± 0.02
2.241MetIle: 2.241 ± 0.051
2.261MetLys: 2.261 ± 0.039
2.502MetLeu: 2.502 ± 0.048
0.824MetMet: 0.824 ± 0.031
1.511MetAsn: 1.511 ± 0.034
0.959MetPro: 0.959 ± 0.03
0.993MetGln: 0.993 ± 0.034
1.018MetArg: 1.018 ± 0.031
1.562MetSer: 1.562 ± 0.028
1.589MetThr: 1.589 ± 0.033
1.792MetVal: 1.792 ± 0.04
0.199MetTrp: 0.199 ± 0.013
0.797MetTyr: 0.797 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.78AsnAla: 2.78 ± 0.05
0.25AsnCys: 0.25 ± 0.014
2.796AsnAsp: 2.796 ± 0.051
3.789AsnGlu: 3.789 ± 0.06
1.889AsnPhe: 1.889 ± 0.04
3.358AsnGly: 3.358 ± 0.063
1.197AsnHis: 1.197 ± 0.03
3.881AsnIle: 3.881 ± 0.064
3.368AsnLys: 3.368 ± 0.058
3.908AsnLeu: 3.908 ± 0.058
1.267AsnMet: 1.267 ± 0.034
2.622AsnAsn: 2.622 ± 0.055
2.081AsnPro: 2.081 ± 0.045
2.734AsnGln: 2.734 ± 0.053
2.084AsnArg: 2.084 ± 0.047
2.468AsnSer: 2.468 ± 0.053
2.423AsnThr: 2.423 ± 0.047
3.201AsnVal: 3.201 ± 0.06
0.701AsnTrp: 0.701 ± 0.028
2.035AsnTyr: 2.035 ± 0.051
0.0AsnXaa: 0.0 ± 0.0
Pro
2.019ProAla: 2.019 ± 0.05
0.184ProCys: 0.184 ± 0.012
2.04ProAsp: 2.04 ± 0.046
2.945ProGlu: 2.945 ± 0.061
1.894ProPhe: 1.894 ± 0.041
2.074ProGly: 2.074 ± 0.047
0.739ProHis: 0.739 ± 0.028
2.974ProIle: 2.974 ± 0.051
1.983ProLys: 1.983 ± 0.046
3.269ProLeu: 3.269 ± 0.056
0.754ProMet: 0.754 ± 0.023
1.876ProAsn: 1.876 ± 0.047
0.871ProPro: 0.871 ± 0.034
1.111ProGln: 1.111 ± 0.035
0.926ProArg: 0.926 ± 0.031
2.241ProSer: 2.241 ± 0.046
2.059ProThr: 2.059 ± 0.04
2.59ProVal: 2.59 ± 0.047
0.399ProTrp: 0.399 ± 0.018
1.382ProTyr: 1.382 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.905GlnAla: 2.905 ± 0.064
0.162GlnCys: 0.162 ± 0.013
2.031GlnAsp: 2.031 ± 0.043
3.409GlnGlu: 3.409 ± 0.063
1.815GlnPhe: 1.815 ± 0.041
2.217GlnGly: 2.217 ± 0.038
0.917GlnHis: 0.917 ± 0.034
3.08GlnIle: 3.08 ± 0.049
2.997GlnLys: 2.997 ± 0.058
4.747GlnLeu: 4.747 ± 0.071
1.25GlnMet: 1.25 ± 0.037
1.955GlnAsn: 1.955 ± 0.048
1.354GlnPro: 1.354 ± 0.038
2.437GlnGln: 2.437 ± 0.066
1.466GlnArg: 1.466 ± 0.041
2.567GlnSer: 2.567 ± 0.055
2.346GlnThr: 2.346 ± 0.048
2.658GlnVal: 2.658 ± 0.048
0.477GlnTrp: 0.477 ± 0.021
1.548GlnTyr: 1.548 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
2.099ArgAla: 2.099 ± 0.045
0.2ArgCys: 0.2 ± 0.013
2.03ArgAsp: 2.03 ± 0.053
2.878ArgGlu: 2.878 ± 0.061
1.787ArgPhe: 1.787 ± 0.042
2.025ArgGly: 2.025 ± 0.045
0.772ArgHis: 0.772 ± 0.026
3.025ArgIle: 3.025 ± 0.057
2.914ArgLys: 2.914 ± 0.06
3.584ArgLeu: 3.584 ± 0.063
1.179ArgMet: 1.179 ± 0.031
1.89ArgAsn: 1.89 ± 0.038
1.127ArgPro: 1.127 ± 0.035
1.462ArgGln: 1.462 ± 0.036
1.449ArgArg: 1.449 ± 0.038
2.067ArgSer: 2.067 ± 0.044
1.866ArgThr: 1.866 ± 0.041
2.425ArgVal: 2.425 ± 0.047
0.398ArgTrp: 0.398 ± 0.02
1.538ArgTyr: 1.538 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
3.383SerAla: 3.383 ± 0.06
0.34SerCys: 0.34 ± 0.017
3.315SerAsp: 3.315 ± 0.062
4.231SerGlu: 4.231 ± 0.068
3.331SerPhe: 3.331 ± 0.061
4.101SerGly: 4.101 ± 0.065
1.214SerHis: 1.214 ± 0.037
5.453SerIle: 5.453 ± 0.071
3.693SerLys: 3.693 ± 0.059
5.897SerLeu: 5.897 ± 0.08
1.631SerMet: 1.631 ± 0.036
3.042SerAsn: 3.042 ± 0.057
1.928SerPro: 1.928 ± 0.036
2.133SerGln: 2.133 ± 0.045
2.016SerArg: 2.016 ± 0.039
3.898SerSer: 3.898 ± 0.081
3.343SerThr: 3.343 ± 0.062
4.089SerVal: 4.089 ± 0.077
0.658SerTrp: 0.658 ± 0.027
2.478SerTyr: 2.478 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
3.554ThrAla: 3.554 ± 0.059
0.328ThrCys: 0.328 ± 0.019
3.133ThrAsp: 3.133 ± 0.06
3.843ThrGlu: 3.843 ± 0.068
2.768ThrPhe: 2.768 ± 0.057
3.818ThrGly: 3.818 ± 0.062
1.032ThrHis: 1.032 ± 0.03
5.182ThrIle: 5.182 ± 0.068
3.451ThrLys: 3.451 ± 0.062
5.27ThrLeu: 5.27 ± 0.073
1.333ThrMet: 1.333 ± 0.034
2.947ThrAsn: 2.947 ± 0.058
2.095ThrPro: 2.095 ± 0.044
1.561ThrGln: 1.561 ± 0.039
1.661ThrArg: 1.661 ± 0.035
3.511ThrSer: 3.511 ± 0.061
3.214ThrThr: 3.214 ± 0.065
4.081ThrVal: 4.081 ± 0.069
0.601ThrTrp: 0.601 ± 0.025
2.152ThrTyr: 2.152 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
4.609ValAla: 4.609 ± 0.075
0.469ValCys: 0.469 ± 0.023
3.952ValAsp: 3.952 ± 0.07
5.264ValGlu: 5.264 ± 0.078
3.149ValPhe: 3.149 ± 0.059
4.23ValGly: 4.23 ± 0.064
1.321ValHis: 1.321 ± 0.035
5.876ValIle: 5.876 ± 0.084
4.272ValLys: 4.272 ± 0.059
6.347ValLeu: 6.347 ± 0.08
1.8ValMet: 1.8 ± 0.042
3.199ValAsn: 3.199 ± 0.06
2.356ValPro: 2.356 ± 0.048
2.339ValGln: 2.339 ± 0.045
2.328ValArg: 2.328 ± 0.05
4.479ValSer: 4.479 ± 0.063
4.128ValThr: 4.128 ± 0.063
4.767ValVal: 4.767 ± 0.067
0.646ValTrp: 0.646 ± 0.026
2.31ValTyr: 2.31 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.572TrpAla: 0.572 ± 0.025
0.07TrpCys: 0.07 ± 0.008
0.618TrpAsp: 0.618 ± 0.023
0.772TrpGlu: 0.772 ± 0.03
0.526TrpPhe: 0.526 ± 0.022
0.648TrpGly: 0.648 ± 0.024
0.262TrpHis: 0.262 ± 0.016
0.937TrpIle: 0.937 ± 0.032
0.759TrpLys: 0.759 ± 0.027
1.272TrpLeu: 1.272 ± 0.031
0.391TrpMet: 0.391 ± 0.017
0.642TrpAsn: 0.642 ± 0.023
0.291TrpPro: 0.291 ± 0.018
0.561TrpGln: 0.561 ± 0.02
0.439TrpArg: 0.439 ± 0.021
0.66TrpSer: 0.66 ± 0.028
0.593TrpThr: 0.593 ± 0.024
0.65TrpVal: 0.65 ± 0.026
0.174TrpTrp: 0.174 ± 0.013
0.426TrpTyr: 0.426 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.079TyrAla: 2.079 ± 0.047
0.257TyrCys: 0.257 ± 0.015
2.171TyrAsp: 2.171 ± 0.069
2.346TyrGlu: 2.346 ± 0.047
1.985TyrPhe: 1.985 ± 0.045
2.422TyrGly: 2.422 ± 0.053
1.038TyrHis: 1.038 ± 0.034
2.709TyrIle: 2.709 ± 0.057
1.985TyrLys: 1.985 ± 0.048
3.747TyrLeu: 3.747 ± 0.067
0.863TyrMet: 0.863 ± 0.028
1.635TyrAsn: 1.635 ± 0.043
1.462TyrPro: 1.462 ± 0.036
2.486TyrGln: 2.486 ± 0.054
1.668TyrArg: 1.668 ± 0.047
2.16TyrSer: 2.16 ± 0.045
1.866TyrThr: 1.866 ± 0.049
2.303TyrVal: 2.303 ± 0.049
0.461TyrTrp: 0.461 ± 0.018
1.659TyrTyr: 1.659 ± 0.044
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3690 proteins (1118330 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski