Tobacco leaf curl Zimbabwe virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q77I78|Q77I78_9GEMI Capsid protein OS=Tobacco leaf curl Zimbabwe virus OX=223337 GN=V2 PE=3 SV=1
MM1 pKa = 7.6GNLISTCLFSSRR13 pKa = 11.84EE14 pKa = 3.86NSTARR19 pKa = 11.84ITDD22 pKa = 3.02SSTWYY27 pKa = 8.11PQPGQHH33 pKa = 6.78ISIQTFRR40 pKa = 11.84EE41 pKa = 4.11LNQAPTSSPTSTRR54 pKa = 11.84MEE56 pKa = 3.82THH58 pKa = 6.72LNGEE62 pKa = 4.35NSRR65 pKa = 11.84STDD68 pKa = 3.25EE69 pKa = 4.21VLEE72 pKa = 4.15EE73 pKa = 4.08AARR76 pKa = 11.84MLTTHH81 pKa = 7.31APTLL85 pKa = 3.78

Molecular weight: 9.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q91M88|Q91M88_9GEMI Replication-associated protein OS=Tobacco leaf curl Zimbabwe virus OX=223337 GN=C1 PE=3 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.44RR4 pKa = 11.84PGDD7 pKa = 3.95IIISTPVSKK16 pKa = 10.56VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.09SPYY27 pKa = 8.55TNRR30 pKa = 11.84AAVPIVQGISKK41 pKa = 9.76RR42 pKa = 11.84RR43 pKa = 11.84SWTYY47 pKa = 9.41RR48 pKa = 11.84PSYY51 pKa = 9.78RR52 pKa = 11.84KK53 pKa = 8.69PRR55 pKa = 11.84MYY57 pKa = 10.91RR58 pKa = 11.84MYY60 pKa = 10.44RR61 pKa = 11.84SPDD64 pKa = 3.21VPRR67 pKa = 11.84GCEE70 pKa = 4.4GPCKK74 pKa = 9.97IQSFDD79 pKa = 3.31QRR81 pKa = 11.84DD82 pKa = 4.17SVVHH86 pKa = 6.3TGTVRR91 pKa = 11.84CLSDD95 pKa = 3.12VTRR98 pKa = 11.84GGGLTHH104 pKa = 6.43RR105 pKa = 11.84TGKK108 pKa = 10.06RR109 pKa = 11.84FCIKK113 pKa = 10.23SIYY116 pKa = 9.86VLGKK120 pKa = 8.9IWMDD124 pKa = 3.44EE125 pKa = 3.92NVKK128 pKa = 10.47KK129 pKa = 10.93SNHH132 pKa = 5.83TNTCMFWVVRR142 pKa = 11.84DD143 pKa = 3.77RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 10.52GPSPQDD153 pKa = 3.16FGQVFNMFDD162 pKa = 3.99NEE164 pKa = 4.13PSTATVQNDD173 pKa = 2.6KK174 pKa = 10.71RR175 pKa = 11.84DD176 pKa = 3.49RR177 pKa = 11.84YY178 pKa = 9.21QVLRR182 pKa = 11.84RR183 pKa = 11.84FQVTVTGGPSGCKK196 pKa = 8.84EE197 pKa = 3.5AAIVKK202 pKa = 10.02RR203 pKa = 11.84FFRR206 pKa = 11.84LNHH209 pKa = 5.74HH210 pKa = 5.45VTYY213 pKa = 10.77NHH215 pKa = 6.09QEE217 pKa = 3.49AAKK220 pKa = 10.4YY221 pKa = 9.25EE222 pKa = 4.13NHH224 pKa = 6.46TEE226 pKa = 3.99NALLLYY232 pKa = 7.29MACTHH237 pKa = 7.07ASNPVYY243 pKa = 10.67ASLKK247 pKa = 8.7IRR249 pKa = 11.84MYY251 pKa = 10.75FYY253 pKa = 11.17DD254 pKa = 3.64SVSNN258 pKa = 3.9

Molecular weight: 29.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1087

85

359

181.2

20.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.336 ± 0.543

2.484 ± 0.423

5.152 ± 0.339

4.692 ± 0.884

4.508 ± 0.523

5.152 ± 0.491

4.508 ± 0.805

5.704 ± 0.873

5.612 ± 0.673

7.452 ± 1.192

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.656 ± 0.5

5.428 ± 0.506

6.072 ± 0.877

4.6 ± 0.41

6.808 ± 1.229

7.636 ± 1.605

6.44 ± 1.109

5.152 ± 1.185

1.564 ± 0.236

4.048 ± 0.521
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski