Aspergillus sclerotialis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Polypaecilum

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11306 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A2Z7I2|A0A3A2Z7I2_9EURO Cysteinyl-tRNA synthetase OS=Aspergillus sclerotialis OX=2070753 GN=PHISCL_08600 PE=4 SV=1
LL1 pKa = 7.95YY2 pKa = 10.56NITDD6 pKa = 3.5GDD8 pKa = 3.71KK9 pKa = 10.98SVAGNEE15 pKa = 3.94LGIVQSLGDD24 pKa = 3.8VYY26 pKa = 11.21SQEE29 pKa = 4.89DD30 pKa = 3.41FDD32 pKa = 6.21LFFTSVAPQIPTGTHH47 pKa = 6.47PNLKK51 pKa = 10.33AIGDD55 pKa = 3.95IVDD58 pKa = 3.56VPTTDD63 pKa = 3.15VSKK66 pKa = 11.06SGSEE70 pKa = 3.84SSLDD74 pKa = 3.57FEE76 pKa = 4.47VAYY79 pKa = 9.23PIIWPQNTVLFQTEE93 pKa = 4.53SEE95 pKa = 4.23SHH97 pKa = 7.15DD98 pKa = 3.14NHH100 pKa = 6.64TYY102 pKa = 10.8GGFVNNLLEE111 pKa = 5.34AIDD114 pKa = 4.75GSYY117 pKa = 11.16CSDD120 pKa = 3.36TDD122 pKa = 4.33DD123 pKa = 4.53EE124 pKa = 4.79GGKK127 pKa = 10.86GKK129 pKa = 9.35DD130 pKa = 2.79QCGIYY135 pKa = 10.35KK136 pKa = 7.31PTNVISISS144 pKa = 3.35

Molecular weight: 15.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A2Z6I9|A0A3A2Z6I9_9EURO DNA repair protein Dds20 (Fragment) OS=Aspergillus sclerotialis OX=2070753 GN=PHISCL_09360 PE=4 SV=1
WW1 pKa = 8.04SMLVMLTKK9 pKa = 10.3TRR11 pKa = 11.84IWMILRR17 pKa = 11.84IGWVGTLRR25 pKa = 11.84IRR27 pKa = 11.84VMMTRR32 pKa = 11.84MRR34 pKa = 11.84VTRR37 pKa = 11.84TVRR40 pKa = 11.84AIVTARR46 pKa = 11.84SPVRR50 pKa = 11.84MRR52 pKa = 11.84RR53 pKa = 11.84IRR55 pKa = 11.84SPSLGPRR62 pKa = 11.84GSVLHH67 pKa = 6.19PRR69 pKa = 11.84RR70 pKa = 11.84NPGRR74 pKa = 11.84KK75 pKa = 9.39DD76 pKa = 3.15LVLRR80 pKa = 11.84LSMKK84 pKa = 10.58LKK86 pKa = 8.98AQEE89 pKa = 4.62RR90 pKa = 11.84RR91 pKa = 11.84ASWRR95 pKa = 3.36

Molecular weight: 11.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11306

0

11306

4755854

49

6861

420.6

46.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.966 ± 0.019

1.22 ± 0.008

5.766 ± 0.018

6.297 ± 0.024

3.797 ± 0.014

6.821 ± 0.021

2.376 ± 0.01

5.075 ± 0.015

4.905 ± 0.018

8.869 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.216 ± 0.008

3.92 ± 0.012

6.079 ± 0.027

3.984 ± 0.015

6.131 ± 0.02

8.441 ± 0.028

5.779 ± 0.016

6.09 ± 0.017

1.426 ± 0.008

2.843 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski