Hafnia phage vB_HpaM_yong1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D2LFR6|A0A7D2LFR6_9CAUD Uncharacterized protein OS=Hafnia phage vB_HpaM_yong1 OX=2719181 PE=4 SV=1
MM1 pKa = 7.6GFPSPAADD9 pKa = 3.41YY10 pKa = 10.68VEE12 pKa = 5.1KK13 pKa = 10.37PISLDD18 pKa = 3.29EE19 pKa = 4.06QFIKK23 pKa = 10.73HH24 pKa = 5.78PAATYY29 pKa = 10.09FMKK32 pKa = 10.84CPDD35 pKa = 3.73YY36 pKa = 10.62CQSAGVLKK44 pKa = 10.72DD45 pKa = 3.41ALLVIDD51 pKa = 3.74SLKK54 pKa = 10.75RR55 pKa = 11.84PVHH58 pKa = 5.68GSVVVAEE65 pKa = 4.35VCGEE69 pKa = 3.93FVLRR73 pKa = 11.84RR74 pKa = 11.84LLTMPVPCLAKK85 pKa = 10.58LEE87 pKa = 4.64NYY89 pKa = 10.75DD90 pKa = 4.59DD91 pKa = 3.73VTFTDD96 pKa = 3.96EE97 pKa = 4.04EE98 pKa = 4.7TGFEE102 pKa = 3.88IFGVVTYY109 pKa = 9.56VVNDD113 pKa = 3.38MSMSEE118 pKa = 4.51FDD120 pKa = 5.98DD121 pKa = 4.34NPCMM125 pKa = 4.97

Molecular weight: 13.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D2HGU2|A0A7D2HGU2_9CAUD Uncharacterized protein OS=Hafnia phage vB_HpaM_yong1 OX=2719181 PE=4 SV=1
MM1 pKa = 7.83ASRR4 pKa = 11.84GVIDD8 pKa = 5.3LYY10 pKa = 11.22SSGMSIPQVSSITGLPRR27 pKa = 11.84STVRR31 pKa = 11.84NQCKK35 pKa = 9.78KK36 pKa = 10.81AGILRR41 pKa = 11.84SRR43 pKa = 11.84ADD45 pKa = 4.11GIRR48 pKa = 11.84KK49 pKa = 9.01AVEE52 pKa = 3.65EE53 pKa = 4.42GRR55 pKa = 11.84LGSNKK60 pKa = 9.08GKK62 pKa = 10.37KK63 pKa = 9.76RR64 pKa = 11.84IFSEE68 pKa = 3.93VWKK71 pKa = 10.64RR72 pKa = 11.84NISAGKK78 pKa = 9.7LKK80 pKa = 10.63HH81 pKa = 6.42ADD83 pKa = 3.32EE84 pKa = 4.36NARR87 pKa = 11.84GYY89 pKa = 10.69RR90 pKa = 11.84ITSTGYY96 pKa = 9.88KK97 pKa = 9.23EE98 pKa = 4.31FTRR101 pKa = 11.84GEE103 pKa = 3.9HH104 pKa = 6.37KK105 pKa = 10.6GRR107 pKa = 11.84SEE109 pKa = 3.92HH110 pKa = 4.97VVIMEE115 pKa = 4.25SIIGRR120 pKa = 11.84RR121 pKa = 11.84IEE123 pKa = 4.25RR124 pKa = 11.84NEE126 pKa = 3.97HH127 pKa = 4.28VHH129 pKa = 6.76HH130 pKa = 7.4KK131 pKa = 10.59DD132 pKa = 3.1RR133 pKa = 11.84NKK135 pKa = 10.96LNNHH139 pKa = 6.64PSNLQLLTISEE150 pKa = 4.38HH151 pKa = 6.06AALHH155 pKa = 5.78RR156 pKa = 11.84KK157 pKa = 8.72EE158 pKa = 4.59DD159 pKa = 3.46AAAGITRR166 pKa = 11.84RR167 pKa = 11.84RR168 pKa = 11.84NKK170 pKa = 10.44NGTWSS175 pKa = 3.2

Molecular weight: 19.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

13251

37

662

174.4

19.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.958 ± 0.312

1.019 ± 0.116

6.083 ± 0.214

6.181 ± 0.301

3.381 ± 0.163

7.343 ± 0.293

1.57 ± 0.135

6.362 ± 0.294

6.233 ± 0.311

7.811 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.875 ± 0.183

4.943 ± 0.223

4.045 ± 0.226

4.166 ± 0.208

5.411 ± 0.287

6.528 ± 0.212

5.969 ± 0.319

6.407 ± 0.235

1.728 ± 0.148

2.988 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski