Stenotrophomonas phage IME15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teseptimavirus; Stenotrophomonas virus IME15

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4PB10|K4PB10_9CAUD DNA ligase OS=Stenotrophomonas phage IME15 OX=1239382 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.17YY9 pKa = 10.19EE10 pKa = 4.4LLAATVEE17 pKa = 4.25AFNEE21 pKa = 4.55RR22 pKa = 11.84IQEE25 pKa = 4.18DD26 pKa = 4.42QLTEE30 pKa = 3.62HH31 pKa = 7.12HH32 pKa = 7.54DD33 pKa = 3.64YY34 pKa = 11.27HH35 pKa = 7.1DD36 pKa = 4.33ALHH39 pKa = 6.06EE40 pKa = 4.37VVDD43 pKa = 4.2RR44 pKa = 11.84MVPHH48 pKa = 6.58YY49 pKa = 9.18YY50 pKa = 9.65WEE52 pKa = 4.46IFTVMAADD60 pKa = 5.31GIDD63 pKa = 3.74VEE65 pKa = 5.01FDD67 pKa = 3.56DD68 pKa = 5.72AGLMPDD74 pKa = 3.53TKK76 pKa = 10.9DD77 pKa = 3.13VTRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 8.84EE88 pKa = 4.09ALYY91 pKa = 10.88NDD93 pKa = 3.88VPSDD97 pKa = 4.56SGIEE101 pKa = 3.85WYY103 pKa = 10.08EE104 pKa = 4.05GEE106 pKa = 4.18EE107 pKa = 4.11EE108 pKa = 4.42EE109 pKa = 5.43EE110 pKa = 4.37PEE112 pKa = 5.32EE113 pKa = 4.16PCQWYY118 pKa = 10.22VINDD122 pKa = 3.85EE123 pKa = 4.14EE124 pKa = 4.72SGPHH128 pKa = 4.4VVAYY132 pKa = 10.35YY133 pKa = 10.82DD134 pKa = 3.55SLEE137 pKa = 4.16AAIEE141 pKa = 4.15AANEE145 pKa = 4.13RR146 pKa = 11.84YY147 pKa = 7.68NTLGKK152 pKa = 10.02LCHH155 pKa = 6.99VEE157 pKa = 4.89DD158 pKa = 4.24DD159 pKa = 3.85TSSEE163 pKa = 3.97VLYY166 pKa = 11.19NPVPTDD172 pKa = 3.29EE173 pKa = 4.31

Molecular weight: 20.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4NZV3|K4NZV3_9CAUD Endonuclease OS=Stenotrophomonas phage IME15 OX=1239382 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.5FKK4 pKa = 10.88LHH6 pKa = 4.51YY7 pKa = 10.24HH8 pKa = 6.53KK9 pKa = 10.59LLKK12 pKa = 10.54HH13 pKa = 4.65FTVRR17 pKa = 11.84RR18 pKa = 11.84SDD20 pKa = 2.85TRR22 pKa = 11.84AIVFTTEE29 pKa = 3.23RR30 pKa = 11.84RR31 pKa = 11.84FVVPLIGQTVEE42 pKa = 3.85LAPNVHH48 pKa = 6.67VIITRR53 pKa = 11.84GKK55 pKa = 9.42FQEE58 pKa = 4.33ATNKK62 pKa = 7.83TRR64 pKa = 11.84PVLNVAVTRR73 pKa = 11.84FPFVKK78 pKa = 10.45LLIEE82 pKa = 4.11RR83 pKa = 11.84VKK85 pKa = 10.86EE86 pKa = 3.9VLKK89 pKa = 11.11

Molecular weight: 10.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

11611

40

1320

258.0

28.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.93 ± 0.383

0.947 ± 0.154

6.089 ± 0.258

7.596 ± 0.372

3.686 ± 0.189

7.381 ± 0.363

1.852 ± 0.19

4.857 ± 0.193

6.347 ± 0.3

8.061 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.911 ± 0.173

4.332 ± 0.244

3.583 ± 0.169

3.945 ± 0.341

5.874 ± 0.222

5.753 ± 0.263

5.443 ± 0.237

6.606 ± 0.295

1.593 ± 0.161

3.212 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski