Amino acid dipepetide frequency for Stenotrophomonas phage IME15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.939AlaAla: 10.939 ± 1.328
0.947AlaCys: 0.947 ± 0.296
5.857AlaAsp: 5.857 ± 0.644
7.063AlaGlu: 7.063 ± 0.643
3.187AlaPhe: 3.187 ± 0.499
7.752AlaGly: 7.752 ± 0.851
1.55AlaHis: 1.55 ± 0.23
5.34AlaIle: 5.34 ± 0.739
6.632AlaLys: 6.632 ± 0.602
8.527AlaLeu: 8.527 ± 0.907
3.359AlaMet: 3.359 ± 0.579
4.393AlaAsn: 4.393 ± 0.647
2.67AlaPro: 2.67 ± 0.528
3.79AlaGln: 3.79 ± 0.805
6.288AlaArg: 6.288 ± 0.771
5.512AlaSer: 5.512 ± 0.723
4.307AlaThr: 4.307 ± 0.588
5.857AlaVal: 5.857 ± 0.924
1.895AlaTrp: 1.895 ± 0.46
3.445AlaTyr: 3.445 ± 0.519
0.0AlaXaa: 0.0 ± 0.0
Cys
0.689CysAla: 0.689 ± 0.276
0.086CysCys: 0.086 ± 0.088
0.689CysAsp: 0.689 ± 0.369
0.345CysGlu: 0.345 ± 0.168
0.431CysPhe: 0.431 ± 0.238
0.431CysGly: 0.431 ± 0.236
0.517CysHis: 0.517 ± 0.232
0.172CysIle: 0.172 ± 0.131
0.517CysLys: 0.517 ± 0.234
0.431CysLeu: 0.431 ± 0.214
0.258CysMet: 0.258 ± 0.15
0.345CysAsn: 0.345 ± 0.183
0.345CysPro: 0.345 ± 0.185
0.603CysGln: 0.603 ± 0.173
0.603CysArg: 0.603 ± 0.255
0.861CysSer: 0.861 ± 0.296
0.258CysThr: 0.258 ± 0.15
0.861CysVal: 0.861 ± 0.264
0.431CysTrp: 0.431 ± 0.228
0.603CysTyr: 0.603 ± 0.225
0.0CysXaa: 0.0 ± 0.0
Asp
5.685AspAla: 5.685 ± 0.696
0.603AspCys: 0.603 ± 0.257
4.307AspAsp: 4.307 ± 0.717
4.823AspGlu: 4.823 ± 0.491
3.101AspPhe: 3.101 ± 0.425
5.599AspGly: 5.599 ± 0.59
0.861AspHis: 0.861 ± 0.264
3.101AspIle: 3.101 ± 0.443
3.618AspLys: 3.618 ± 0.492
3.359AspLeu: 3.359 ± 0.627
1.723AspMet: 1.723 ± 0.308
1.378AspAsn: 1.378 ± 0.323
3.015AspPro: 3.015 ± 0.57
1.809AspGln: 1.809 ± 0.424
2.67AspArg: 2.67 ± 0.57
4.048AspSer: 4.048 ± 0.529
4.307AspThr: 4.307 ± 0.581
4.393AspVal: 4.393 ± 0.633
0.689AspTrp: 0.689 ± 0.308
1.809AspTyr: 1.809 ± 0.369
0.0AspXaa: 0.0 ± 0.0
Glu
7.924GluAla: 7.924 ± 0.985
0.431GluCys: 0.431 ± 0.221
4.393GluAsp: 4.393 ± 0.646
6.632GluGlu: 6.632 ± 1.013
2.584GluPhe: 2.584 ± 0.504
4.651GluGly: 4.651 ± 0.671
1.378GluHis: 1.378 ± 0.38
3.531GluIle: 3.531 ± 0.402
3.618GluLys: 3.618 ± 0.659
7.838GluLeu: 7.838 ± 1.015
2.153GluMet: 2.153 ± 0.352
2.842GluAsn: 2.842 ± 0.396
2.153GluPro: 2.153 ± 0.459
4.048GluGln: 4.048 ± 0.726
4.737GluArg: 4.737 ± 0.556
4.479GluSer: 4.479 ± 0.486
3.618GluThr: 3.618 ± 0.458
5.34GluVal: 5.34 ± 0.613
0.861GluTrp: 0.861 ± 0.296
2.756GluTyr: 2.756 ± 0.452
0.0GluXaa: 0.0 ± 0.0
Phe
2.67PheAla: 2.67 ± 0.353
0.517PheCys: 0.517 ± 0.211
2.756PheAsp: 2.756 ± 0.516
2.756PheGlu: 2.756 ± 0.532
1.12PhePhe: 1.12 ± 0.263
3.273PheGly: 3.273 ± 0.48
0.603PheHis: 0.603 ± 0.277
1.55PheIle: 1.55 ± 0.473
2.239PheLys: 2.239 ± 0.394
3.101PheLeu: 3.101 ± 0.527
1.378PheMet: 1.378 ± 0.238
2.153PheAsn: 2.153 ± 0.469
1.637PhePro: 1.637 ± 0.387
1.378PheGln: 1.378 ± 0.392
1.895PheArg: 1.895 ± 0.346
1.895PheSer: 1.895 ± 0.456
2.842PheThr: 2.842 ± 0.52
1.809PheVal: 1.809 ± 0.353
0.431PheTrp: 0.431 ± 0.164
0.861PheTyr: 0.861 ± 0.272
0.0PheXaa: 0.0 ± 0.0
Gly
7.149GlyAla: 7.149 ± 0.874
1.034GlyCys: 1.034 ± 0.369
5.082GlyAsp: 5.082 ± 0.677
5.168GlyGlu: 5.168 ± 0.725
3.618GlyPhe: 3.618 ± 0.457
6.029GlyGly: 6.029 ± 0.723
1.723GlyHis: 1.723 ± 0.317
4.651GlyIle: 4.651 ± 0.565
5.254GlyLys: 5.254 ± 0.735
5.512GlyLeu: 5.512 ± 0.857
1.55GlyMet: 1.55 ± 0.41
2.756GlyAsn: 2.756 ± 0.445
1.12GlyPro: 1.12 ± 0.308
3.273GlyGln: 3.273 ± 0.434
4.134GlyArg: 4.134 ± 0.546
3.618GlySer: 3.618 ± 0.63
3.359GlyThr: 3.359 ± 0.546
4.393GlyVal: 4.393 ± 0.693
1.637GlyTrp: 1.637 ± 0.389
2.756GlyTyr: 2.756 ± 0.348
0.0GlyXaa: 0.0 ± 0.0
His
1.55HisAla: 1.55 ± 0.329
0.258HisCys: 0.258 ± 0.155
1.206HisAsp: 1.206 ± 0.294
1.464HisGlu: 1.464 ± 0.415
0.947HisPhe: 0.947 ± 0.258
1.292HisGly: 1.292 ± 0.317
0.689HisHis: 0.689 ± 0.224
1.378HisIle: 1.378 ± 0.367
1.292HisLys: 1.292 ± 0.344
1.637HisLeu: 1.637 ± 0.514
0.603HisMet: 0.603 ± 0.195
0.689HisAsn: 0.689 ± 0.301
0.258HisPro: 0.258 ± 0.136
0.345HisGln: 0.345 ± 0.179
0.947HisArg: 0.947 ± 0.226
0.861HisSer: 0.861 ± 0.262
0.431HisThr: 0.431 ± 0.183
1.378HisVal: 1.378 ± 0.298
0.431HisTrp: 0.431 ± 0.167
0.861HisTyr: 0.861 ± 0.255
0.0HisXaa: 0.0 ± 0.0
Ile
3.79IleAla: 3.79 ± 0.621
0.345IleCys: 0.345 ± 0.136
3.445IleAsp: 3.445 ± 0.429
3.962IleGlu: 3.962 ± 0.571
1.12IlePhe: 1.12 ± 0.25
3.187IleGly: 3.187 ± 0.511
1.12IleHis: 1.12 ± 0.415
2.326IleIle: 2.326 ± 0.552
2.842IleLys: 2.842 ± 0.456
3.273IleLeu: 3.273 ± 0.495
1.723IleMet: 1.723 ± 0.426
2.326IleAsn: 2.326 ± 0.498
2.584IlePro: 2.584 ± 0.359
2.067IleGln: 2.067 ± 0.477
3.445IleArg: 3.445 ± 0.496
3.273IleSer: 3.273 ± 0.476
2.67IleThr: 2.67 ± 0.466
2.929IleVal: 2.929 ± 0.461
0.517IleTrp: 0.517 ± 0.218
1.637IleTyr: 1.637 ± 0.39
0.0IleXaa: 0.0 ± 0.0
Lys
7.321LysAla: 7.321 ± 0.871
0.603LysCys: 0.603 ± 0.238
3.015LysAsp: 3.015 ± 0.35
4.393LysGlu: 4.393 ± 0.665
2.326LysPhe: 2.326 ± 0.422
5.254LysGly: 5.254 ± 0.633
1.723LysHis: 1.723 ± 0.328
2.326LysIle: 2.326 ± 0.329
3.962LysLys: 3.962 ± 0.802
4.996LysLeu: 4.996 ± 0.769
1.637LysMet: 1.637 ± 0.343
2.584LysAsn: 2.584 ± 0.358
2.929LysPro: 2.929 ± 0.529
2.412LysGln: 2.412 ± 0.396
3.531LysArg: 3.531 ± 0.617
3.101LysSer: 3.101 ± 0.599
3.101LysThr: 3.101 ± 0.419
4.823LysVal: 4.823 ± 0.541
1.034LysTrp: 1.034 ± 0.215
2.412LysTyr: 2.412 ± 0.36
0.0LysXaa: 0.0 ± 0.0
Leu
8.958LeuAla: 8.958 ± 0.939
0.345LeuCys: 0.345 ± 0.187
4.737LeuAsp: 4.737 ± 0.602
6.115LeuGlu: 6.115 ± 0.75
1.895LeuPhe: 1.895 ± 0.44
5.168LeuGly: 5.168 ± 0.946
0.775LeuHis: 0.775 ± 0.245
3.531LeuIle: 3.531 ± 0.588
5.943LeuLys: 5.943 ± 0.799
5.082LeuLeu: 5.082 ± 0.719
2.412LeuMet: 2.412 ± 0.429
4.22LeuAsn: 4.22 ± 0.607
3.445LeuPro: 3.445 ± 0.46
3.962LeuGln: 3.962 ± 0.5
5.857LeuArg: 5.857 ± 0.606
4.048LeuSer: 4.048 ± 0.389
5.082LeuThr: 5.082 ± 0.622
4.996LeuVal: 4.996 ± 0.481
1.206LeuTrp: 1.206 ± 0.426
2.842LeuTyr: 2.842 ± 0.466
0.0LeuXaa: 0.0 ± 0.0
Met
3.445MetAla: 3.445 ± 0.359
0.258MetCys: 0.258 ± 0.151
1.55MetAsp: 1.55 ± 0.309
1.981MetGlu: 1.981 ± 0.432
1.206MetPhe: 1.206 ± 0.321
2.153MetGly: 2.153 ± 0.391
0.431MetHis: 0.431 ± 0.14
1.034MetIle: 1.034 ± 0.243
1.55MetLys: 1.55 ± 0.444
2.842MetLeu: 2.842 ± 0.433
0.775MetMet: 0.775 ± 0.327
1.378MetAsn: 1.378 ± 0.363
1.292MetPro: 1.292 ± 0.321
1.12MetGln: 1.12 ± 0.365
1.378MetArg: 1.378 ± 0.258
1.637MetSer: 1.637 ± 0.385
2.067MetThr: 2.067 ± 0.341
2.153MetVal: 2.153 ± 0.348
0.258MetTrp: 0.258 ± 0.167
0.603MetTyr: 0.603 ± 0.224
0.0MetXaa: 0.0 ± 0.0
Asn
4.134AsnAla: 4.134 ± 0.557
0.603AsnCys: 0.603 ± 0.266
2.153AsnAsp: 2.153 ± 0.349
3.273AsnGlu: 3.273 ± 0.614
1.464AsnPhe: 1.464 ± 0.326
3.704AsnGly: 3.704 ± 0.652
0.689AsnHis: 0.689 ± 0.253
1.895AsnIle: 1.895 ± 0.427
2.584AsnLys: 2.584 ± 0.427
3.962AsnLeu: 3.962 ± 0.619
1.464AsnMet: 1.464 ± 0.353
1.637AsnAsn: 1.637 ± 0.434
2.412AsnPro: 2.412 ± 0.343
1.55AsnGln: 1.55 ± 0.355
2.584AsnArg: 2.584 ± 0.608
2.498AsnSer: 2.498 ± 0.545
1.981AsnThr: 1.981 ± 0.34
2.756AsnVal: 2.756 ± 0.467
0.517AsnTrp: 0.517 ± 0.179
1.464AsnTyr: 1.464 ± 0.37
0.0AsnXaa: 0.0 ± 0.0
Pro
3.015ProAla: 3.015 ± 0.371
0.517ProCys: 0.517 ± 0.192
2.842ProAsp: 2.842 ± 0.445
4.307ProGlu: 4.307 ± 0.622
1.292ProPhe: 1.292 ± 0.37
0.345ProGly: 0.345 ± 0.189
0.861ProHis: 0.861 ± 0.325
1.378ProIle: 1.378 ± 0.325
2.498ProLys: 2.498 ± 0.438
2.842ProLeu: 2.842 ± 0.466
0.947ProMet: 0.947 ± 0.266
2.067ProAsn: 2.067 ± 0.369
0.603ProPro: 0.603 ± 0.207
1.034ProGln: 1.034 ± 0.329
1.292ProArg: 1.292 ± 0.397
2.326ProSer: 2.326 ± 0.475
2.842ProThr: 2.842 ± 0.481
2.584ProVal: 2.584 ± 0.326
0.689ProTrp: 0.689 ± 0.175
1.55ProTyr: 1.55 ± 0.365
0.0ProXaa: 0.0 ± 0.0
Gln
4.91GlnAla: 4.91 ± 0.726
0.172GlnCys: 0.172 ± 0.119
1.895GlnAsp: 1.895 ± 0.326
3.187GlnGlu: 3.187 ± 0.557
2.412GlnPhe: 2.412 ± 0.369
2.929GlnGly: 2.929 ± 0.481
0.689GlnHis: 0.689 ± 0.242
2.239GlnIle: 2.239 ± 0.52
1.809GlnLys: 1.809 ± 0.442
3.876GlnLeu: 3.876 ± 0.48
1.55GlnMet: 1.55 ± 0.365
0.861GlnAsn: 0.861 ± 0.238
0.947GlnPro: 0.947 ± 0.247
1.637GlnGln: 1.637 ± 0.308
2.67GlnArg: 2.67 ± 0.591
1.981GlnSer: 1.981 ± 0.342
1.637GlnThr: 1.637 ± 0.508
2.326GlnVal: 2.326 ± 0.449
1.034GlnTrp: 1.034 ± 0.301
0.603GlnTyr: 0.603 ± 0.318
0.0GlnXaa: 0.0 ± 0.0
Arg
6.115ArgAla: 6.115 ± 0.777
1.034ArgCys: 1.034 ± 0.311
3.704ArgAsp: 3.704 ± 0.556
4.565ArgGlu: 4.565 ± 0.564
2.412ArgPhe: 2.412 ± 0.57
3.79ArgGly: 3.79 ± 0.506
1.034ArgHis: 1.034 ± 0.352
2.67ArgIle: 2.67 ± 0.46
4.651ArgLys: 4.651 ± 0.767
4.737ArgLeu: 4.737 ± 0.484
1.723ArgMet: 1.723 ± 0.426
2.842ArgAsn: 2.842 ± 0.527
1.981ArgPro: 1.981 ± 0.303
2.153ArgGln: 2.153 ± 0.428
2.842ArgArg: 2.842 ± 0.419
4.737ArgSer: 4.737 ± 0.762
2.412ArgThr: 2.412 ± 0.351
3.101ArgVal: 3.101 ± 0.334
0.775ArgTrp: 0.775 ± 0.331
1.464ArgTyr: 1.464 ± 0.37
0.0ArgXaa: 0.0 ± 0.0
Ser
5.943SerAla: 5.943 ± 0.665
0.258SerCys: 0.258 ± 0.167
3.876SerAsp: 3.876 ± 0.548
3.187SerGlu: 3.187 ± 0.391
2.584SerPhe: 2.584 ± 0.293
5.34SerGly: 5.34 ± 0.591
1.12SerHis: 1.12 ± 0.275
2.239SerIle: 2.239 ± 0.414
3.531SerLys: 3.531 ± 0.561
3.876SerLeu: 3.876 ± 0.54
1.292SerMet: 1.292 ± 0.356
2.67SerAsn: 2.67 ± 0.421
1.809SerPro: 1.809 ± 0.407
2.326SerGln: 2.326 ± 0.404
3.876SerArg: 3.876 ± 0.62
2.929SerSer: 2.929 ± 0.507
3.79SerThr: 3.79 ± 0.637
3.79SerVal: 3.79 ± 0.515
1.034SerTrp: 1.034 ± 0.245
2.067SerTyr: 2.067 ± 0.528
0.0SerXaa: 0.0 ± 0.0
Thr
4.651ThrAla: 4.651 ± 0.707
0.431ThrCys: 0.431 ± 0.197
3.273ThrAsp: 3.273 ± 0.457
4.22ThrGlu: 4.22 ± 0.593
1.723ThrPhe: 1.723 ± 0.39
5.512ThrGly: 5.512 ± 0.814
0.861ThrHis: 0.861 ± 0.224
3.101ThrIle: 3.101 ± 0.552
4.22ThrLys: 4.22 ± 0.553
5.426ThrLeu: 5.426 ± 0.686
1.206ThrMet: 1.206 ± 0.305
2.067ThrAsn: 2.067 ± 0.384
2.756ThrPro: 2.756 ± 0.486
1.809ThrGln: 1.809 ± 0.316
2.239ThrArg: 2.239 ± 0.495
2.842ThrSer: 2.842 ± 0.489
2.498ThrThr: 2.498 ± 0.435
3.876ThrVal: 3.876 ± 0.771
0.431ThrTrp: 0.431 ± 0.178
1.292ThrTyr: 1.292 ± 0.269
0.0ThrXaa: 0.0 ± 0.0
Val
6.029ValAla: 6.029 ± 0.732
0.517ValCys: 0.517 ± 0.225
3.273ValAsp: 3.273 ± 0.735
4.996ValGlu: 4.996 ± 0.861
2.326ValPhe: 2.326 ± 0.541
4.22ValGly: 4.22 ± 0.713
0.947ValHis: 0.947 ± 0.329
3.962ValIle: 3.962 ± 0.532
3.79ValLys: 3.79 ± 0.638
5.685ValLeu: 5.685 ± 0.66
1.981ValMet: 1.981 ± 0.353
2.842ValAsn: 2.842 ± 0.52
2.67ValPro: 2.67 ± 0.52
2.067ValGln: 2.067 ± 0.373
4.393ValArg: 4.393 ± 0.44
4.479ValSer: 4.479 ± 0.527
4.22ValThr: 4.22 ± 0.548
4.823ValVal: 4.823 ± 0.682
0.947ValTrp: 0.947 ± 0.212
1.895ValTyr: 1.895 ± 0.44
0.0ValXaa: 0.0 ± 0.0
Trp
0.947TrpAla: 0.947 ± 0.267
0.345TrpCys: 0.345 ± 0.171
0.689TrpAsp: 0.689 ± 0.258
0.947TrpGlu: 0.947 ± 0.229
0.517TrpPhe: 0.517 ± 0.226
1.12TrpGly: 1.12 ± 0.339
0.431TrpHis: 0.431 ± 0.251
0.775TrpIle: 0.775 ± 0.32
1.378TrpLys: 1.378 ± 0.323
1.292TrpLeu: 1.292 ± 0.311
0.517TrpMet: 0.517 ± 0.18
1.292TrpAsn: 1.292 ± 0.361
0.172TrpPro: 0.172 ± 0.103
0.775TrpGln: 0.775 ± 0.26
0.689TrpArg: 0.689 ± 0.251
1.034TrpSer: 1.034 ± 0.442
1.034TrpThr: 1.034 ± 0.237
1.292TrpVal: 1.292 ± 0.394
0.345TrpTrp: 0.345 ± 0.206
0.345TrpTyr: 0.345 ± 0.232
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.445TyrAla: 3.445 ± 0.534
0.172TyrCys: 0.172 ± 0.114
2.153TyrAsp: 2.153 ± 0.554
2.584TyrGlu: 2.584 ± 0.456
0.603TyrPhe: 0.603 ± 0.266
2.067TyrGly: 2.067 ± 0.477
0.517TyrHis: 0.517 ± 0.202
1.378TyrIle: 1.378 ± 0.44
1.55TyrLys: 1.55 ± 0.36
2.326TyrLeu: 2.326 ± 0.433
0.775TyrMet: 0.775 ± 0.226
1.981TyrAsn: 1.981 ± 0.442
1.034TyrPro: 1.034 ± 0.262
1.292TyrGln: 1.292 ± 0.369
2.67TyrArg: 2.67 ± 0.506
1.378TyrSer: 1.378 ± 0.419
1.981TyrThr: 1.981 ± 0.327
2.584TyrVal: 2.584 ± 0.575
0.775TyrTrp: 0.775 ± 0.254
0.861TyrTyr: 0.861 ± 0.371
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (11611 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski