Mycobacterium phage Rajelicia

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411C3Z5|A0A411C3Z5_9CAUD HNH endonuclease OS=Mycobacterium phage Rajelicia OX=2502444 GN=2 PE=4 SV=1
MM1 pKa = 7.61GFTLRR6 pKa = 11.84LFGIPVLSLEE16 pKa = 4.13ITGDD20 pKa = 3.64GSAEE24 pKa = 4.06EE25 pKa = 4.65YY26 pKa = 10.37ISLTGGSFEE35 pKa = 4.98LAPEE39 pKa = 4.19EE40 pKa = 3.94PDD42 pKa = 3.33YY43 pKa = 11.68DD44 pKa = 3.65EE45 pKa = 5.6EE46 pKa = 4.97YY47 pKa = 10.93YY48 pKa = 10.93EE49 pKa = 4.28EE50 pKa = 4.6DD51 pKa = 3.1RR52 pKa = 11.84SGFGFGVSS60 pKa = 3.28

Molecular weight: 6.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411C423|A0A411C423_9CAUD Tail assembly chaperone OS=Mycobacterium phage Rajelicia OX=2502444 GN=23 PE=4 SV=1
MM1 pKa = 8.09WIPCLCWAHH10 pKa = 7.06ADD12 pKa = 3.88TRR14 pKa = 11.84PSAAVSYY21 pKa = 10.32KK22 pKa = 10.81LNAFACLGCGTKK34 pKa = 10.62GSAISLLMKK43 pKa = 10.27RR44 pKa = 11.84EE45 pKa = 4.21EE46 pKa = 4.39VSHH49 pKa = 6.64HH50 pKa = 6.46RR51 pKa = 11.84ALEE54 pKa = 4.02LAQEE58 pKa = 4.42LSPGSIEE65 pKa = 4.19AVSPGPARR73 pKa = 11.84LRR75 pKa = 11.84RR76 pKa = 11.84GGVSGNPRR84 pKa = 11.84ANQSSHH90 pKa = 5.73LRR92 pKa = 11.84RR93 pKa = 11.84SEE95 pKa = 3.95QVSPRR100 pKa = 11.84LRR102 pKa = 11.84SGAAARR108 pKa = 11.84AA109 pKa = 3.6

Molecular weight: 11.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

16583

27

1107

186.3

20.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.022 ± 0.416

0.663 ± 0.103

6.531 ± 0.179

6.73 ± 0.29

3.202 ± 0.161

8.666 ± 0.562

2.074 ± 0.173

4.818 ± 0.211

4.263 ± 0.267

8.569 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.008 ± 0.122

3.22 ± 0.153

5.548 ± 0.241

3.1 ± 0.146

6.211 ± 0.428

5.94 ± 0.259

6.386 ± 0.287

7.067 ± 0.185

2.014 ± 0.104

2.967 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski