Amino acid dipepetide frequency for Mycobacterium phage Rajelicia

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.845AlaAla: 12.845 ± 1.157
0.724AlaCys: 0.724 ± 0.207
7.056AlaAsp: 7.056 ± 0.695
6.091AlaGlu: 6.091 ± 0.679
3.437AlaPhe: 3.437 ± 0.469
7.599AlaGly: 7.599 ± 0.753
1.628AlaHis: 1.628 ± 0.31
4.463AlaIle: 4.463 ± 0.547
4.101AlaLys: 4.101 ± 0.541
8.986AlaLeu: 8.986 ± 0.797
2.533AlaMet: 2.533 ± 0.514
2.834AlaAsn: 2.834 ± 0.387
4.825AlaPro: 4.825 ± 0.677
2.895AlaGln: 2.895 ± 0.472
6.091AlaArg: 6.091 ± 0.583
5.608AlaSer: 5.608 ± 0.634
5.91AlaThr: 5.91 ± 0.559
7.84AlaVal: 7.84 ± 0.7
1.749AlaTrp: 1.749 ± 0.326
2.955AlaTyr: 2.955 ± 0.402
0.0AlaXaa: 0.0 ± 0.0
Cys
0.663CysAla: 0.663 ± 0.222
0.0CysCys: 0.0 ± 0.0
0.362CysAsp: 0.362 ± 0.123
0.724CysGlu: 0.724 ± 0.193
0.121CysPhe: 0.121 ± 0.082
0.724CysGly: 0.724 ± 0.218
0.121CysHis: 0.121 ± 0.089
0.181CysIle: 0.181 ± 0.105
0.362CysLys: 0.362 ± 0.16
0.482CysLeu: 0.482 ± 0.199
0.181CysMet: 0.181 ± 0.099
0.302CysAsn: 0.302 ± 0.137
0.362CysPro: 0.362 ± 0.139
0.181CysGln: 0.181 ± 0.124
0.603CysArg: 0.603 ± 0.185
0.302CysSer: 0.302 ± 0.123
0.362CysThr: 0.362 ± 0.143
0.241CysVal: 0.241 ± 0.108
0.241CysTrp: 0.241 ± 0.093
0.121CysTyr: 0.121 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
6.634AspAla: 6.634 ± 0.576
0.663AspCys: 0.663 ± 0.188
4.463AspAsp: 4.463 ± 0.54
3.98AspGlu: 3.98 ± 0.535
2.714AspPhe: 2.714 ± 0.366
5.85AspGly: 5.85 ± 0.664
1.086AspHis: 1.086 ± 0.254
3.015AspIle: 3.015 ± 0.448
2.593AspLys: 2.593 ± 0.391
6.935AspLeu: 6.935 ± 0.676
1.086AspMet: 1.086 ± 0.218
1.749AspAsn: 1.749 ± 0.318
4.764AspPro: 4.764 ± 0.627
1.508AspGln: 1.508 ± 0.294
3.257AspArg: 3.257 ± 0.316
3.136AspSer: 3.136 ± 0.447
3.98AspThr: 3.98 ± 0.438
4.101AspVal: 4.101 ± 0.498
1.689AspTrp: 1.689 ± 0.301
2.111AspTyr: 2.111 ± 0.285
0.0AspXaa: 0.0 ± 0.0
Glu
6.332GluAla: 6.332 ± 0.704
0.302GluCys: 0.302 ± 0.144
4.945GluAsp: 4.945 ± 0.593
5.066GluGlu: 5.066 ± 0.578
2.352GluPhe: 2.352 ± 0.434
3.86GluGly: 3.86 ± 0.447
1.689GluHis: 1.689 ± 0.314
3.437GluIle: 3.437 ± 0.412
3.136GluLys: 3.136 ± 0.44
7.297GluLeu: 7.297 ± 0.599
1.809GluMet: 1.809 ± 0.288
2.05GluAsn: 2.05 ± 0.422
2.533GluPro: 2.533 ± 0.401
2.473GluGln: 2.473 ± 0.372
3.257GluArg: 3.257 ± 0.489
3.498GluSer: 3.498 ± 0.422
3.799GluThr: 3.799 ± 0.547
5.428GluVal: 5.428 ± 0.602
1.508GluTrp: 1.508 ± 0.299
2.533GluTyr: 2.533 ± 0.418
0.0GluXaa: 0.0 ± 0.0
Phe
2.231PheAla: 2.231 ± 0.33
0.241PheCys: 0.241 ± 0.133
2.895PheAsp: 2.895 ± 0.364
2.473PheGlu: 2.473 ± 0.378
0.663PhePhe: 0.663 ± 0.18
3.377PheGly: 3.377 ± 0.405
0.844PheHis: 0.844 ± 0.243
1.628PheIle: 1.628 ± 0.319
1.327PheLys: 1.327 ± 0.296
2.473PheLeu: 2.473 ± 0.37
0.663PheMet: 0.663 ± 0.173
1.025PheAsn: 1.025 ± 0.238
1.749PhePro: 1.749 ± 0.31
1.025PheGln: 1.025 ± 0.223
1.628PheArg: 1.628 ± 0.361
1.809PheSer: 1.809 ± 0.268
2.231PheThr: 2.231 ± 0.307
1.93PheVal: 1.93 ± 0.31
0.603PheTrp: 0.603 ± 0.193
1.206PheTyr: 1.206 ± 0.233
0.0PheXaa: 0.0 ± 0.0
Gly
7.9GlyAla: 7.9 ± 1.138
0.724GlyCys: 0.724 ± 0.245
5.97GlyAsp: 5.97 ± 0.559
4.523GlyGlu: 4.523 ± 0.453
3.076GlyPhe: 3.076 ± 0.449
9.347GlyGly: 9.347 ± 2.608
1.809GlyHis: 1.809 ± 0.321
4.402GlyIle: 4.402 ± 0.536
3.558GlyLys: 3.558 ± 0.472
7.659GlyLeu: 7.659 ± 0.783
1.568GlyMet: 1.568 ± 0.321
3.498GlyAsn: 3.498 ± 0.471
3.618GlyPro: 3.618 ± 0.484
2.231GlyGln: 2.231 ± 0.275
5.066GlyArg: 5.066 ± 0.578
5.548GlySer: 5.548 ± 0.68
5.428GlyThr: 5.428 ± 0.621
5.488GlyVal: 5.488 ± 0.59
2.352GlyTrp: 2.352 ± 0.372
2.895GlyTyr: 2.895 ± 0.356
0.0GlyXaa: 0.0 ± 0.0
His
1.689HisAla: 1.689 ± 0.33
0.121HisCys: 0.121 ± 0.075
1.146HisAsp: 1.146 ± 0.234
1.568HisGlu: 1.568 ± 0.353
0.603HisPhe: 0.603 ± 0.161
1.568HisGly: 1.568 ± 0.326
0.724HisHis: 0.724 ± 0.222
0.965HisIle: 0.965 ± 0.186
0.965HisLys: 0.965 ± 0.317
1.568HisLeu: 1.568 ± 0.338
0.06HisMet: 0.06 ± 0.058
0.241HisAsn: 0.241 ± 0.118
1.447HisPro: 1.447 ± 0.288
1.146HisGln: 1.146 ± 0.244
1.809HisArg: 1.809 ± 0.326
0.844HisSer: 0.844 ± 0.2
1.327HisThr: 1.327 ± 0.319
1.809HisVal: 1.809 ± 0.304
0.482HisTrp: 0.482 ± 0.16
0.663HisTyr: 0.663 ± 0.242
0.0HisXaa: 0.0 ± 0.0
Ile
5.91IleAla: 5.91 ± 0.6
0.302IleCys: 0.302 ± 0.129
3.257IleAsp: 3.257 ± 0.386
3.618IleGlu: 3.618 ± 0.476
0.905IlePhe: 0.905 ± 0.25
3.98IleGly: 3.98 ± 0.381
0.905IleHis: 0.905 ± 0.187
1.568IleIle: 1.568 ± 0.273
1.447IleLys: 1.447 ± 0.301
3.618IleLeu: 3.618 ± 0.444
0.844IleMet: 0.844 ± 0.227
1.749IleAsn: 1.749 ± 0.328
2.955IlePro: 2.955 ± 0.425
1.266IleGln: 1.266 ± 0.291
3.437IleArg: 3.437 ± 0.43
3.98IleSer: 3.98 ± 0.668
3.317IleThr: 3.317 ± 0.41
2.714IleVal: 2.714 ± 0.481
0.663IleTrp: 0.663 ± 0.173
1.749IleTyr: 1.749 ± 0.312
0.0IleXaa: 0.0 ± 0.0
Lys
4.041LysAla: 4.041 ± 0.465
0.241LysCys: 0.241 ± 0.11
2.292LysAsp: 2.292 ± 0.404
2.05LysGlu: 2.05 ± 0.326
1.387LysPhe: 1.387 ± 0.262
2.895LysGly: 2.895 ± 0.402
1.266LysHis: 1.266 ± 0.291
2.473LysIle: 2.473 ± 0.421
1.809LysLys: 1.809 ± 0.412
3.739LysLeu: 3.739 ± 0.511
1.025LysMet: 1.025 ± 0.237
1.628LysAsn: 1.628 ± 0.318
2.774LysPro: 2.774 ± 0.438
1.508LysGln: 1.508 ± 0.298
3.015LysArg: 3.015 ± 0.479
2.473LysSer: 2.473 ± 0.382
2.714LysThr: 2.714 ± 0.403
3.317LysVal: 3.317 ± 0.411
0.844LysTrp: 0.844 ± 0.218
1.146LysTyr: 1.146 ± 0.276
0.0LysXaa: 0.0 ± 0.0
Leu
9.167LeuAla: 9.167 ± 0.819
0.482LeuCys: 0.482 ± 0.203
6.091LeuAsp: 6.091 ± 0.517
5.97LeuGlu: 5.97 ± 0.669
2.111LeuPhe: 2.111 ± 0.32
7.297LeuGly: 7.297 ± 0.667
1.447LeuHis: 1.447 ± 0.331
4.161LeuIle: 4.161 ± 0.528
4.101LeuLys: 4.101 ± 0.442
6.031LeuLeu: 6.031 ± 0.561
1.689LeuMet: 1.689 ± 0.272
3.196LeuAsn: 3.196 ± 0.37
5.729LeuPro: 5.729 ± 0.543
2.292LeuGln: 2.292 ± 0.363
6.091LeuArg: 6.091 ± 0.498
5.548LeuSer: 5.548 ± 0.596
6.151LeuThr: 6.151 ± 0.456
4.704LeuVal: 4.704 ± 0.562
0.965LeuTrp: 0.965 ± 0.291
2.473LeuTyr: 2.473 ± 0.406
0.0LeuXaa: 0.0 ± 0.0
Met
2.05MetAla: 2.05 ± 0.311
0.0MetCys: 0.0 ± 0.0
0.965MetAsp: 0.965 ± 0.224
1.327MetGlu: 1.327 ± 0.335
0.663MetPhe: 0.663 ± 0.182
1.387MetGly: 1.387 ± 0.28
0.543MetHis: 0.543 ± 0.264
0.603MetIle: 0.603 ± 0.195
1.146MetLys: 1.146 ± 0.271
1.086MetLeu: 1.086 ± 0.285
0.06MetMet: 0.06 ± 0.062
0.844MetAsn: 0.844 ± 0.243
1.086MetPro: 1.086 ± 0.254
0.362MetGln: 0.362 ± 0.127
1.628MetArg: 1.628 ± 0.321
2.111MetSer: 2.111 ± 0.381
2.352MetThr: 2.352 ± 0.325
1.025MetVal: 1.025 ± 0.313
0.482MetTrp: 0.482 ± 0.128
0.362MetTyr: 0.362 ± 0.143
0.0MetXaa: 0.0 ± 0.0
Asn
3.196AsnAla: 3.196 ± 0.447
0.0AsnCys: 0.0 ± 0.0
1.93AsnAsp: 1.93 ± 0.347
2.111AsnGlu: 2.111 ± 0.379
1.086AsnPhe: 1.086 ± 0.284
3.257AsnGly: 3.257 ± 0.387
0.663AsnHis: 0.663 ± 0.2
1.749AsnIle: 1.749 ± 0.314
0.543AsnLys: 0.543 ± 0.191
2.292AsnLeu: 2.292 ± 0.275
0.663AsnMet: 0.663 ± 0.173
0.844AsnAsn: 0.844 ± 0.214
2.533AsnPro: 2.533 ± 0.385
1.086AsnGln: 1.086 ± 0.225
1.508AsnArg: 1.508 ± 0.354
1.568AsnSer: 1.568 ± 0.355
2.05AsnThr: 2.05 ± 0.401
2.834AsnVal: 2.834 ± 0.437
0.905AsnTrp: 0.905 ± 0.207
1.387AsnTyr: 1.387 ± 0.231
0.0AsnXaa: 0.0 ± 0.0
Pro
5.186ProAla: 5.186 ± 0.501
0.603ProCys: 0.603 ± 0.215
4.463ProAsp: 4.463 ± 0.457
4.161ProGlu: 4.161 ± 0.51
2.231ProPhe: 2.231 ± 0.332
5.789ProGly: 5.789 ± 0.731
0.905ProHis: 0.905 ± 0.212
2.231ProIle: 2.231 ± 0.317
2.111ProLys: 2.111 ± 0.309
4.583ProLeu: 4.583 ± 0.521
0.784ProMet: 0.784 ± 0.233
1.568ProAsn: 1.568 ± 0.333
3.015ProPro: 3.015 ± 0.517
1.508ProGln: 1.508 ± 0.334
2.955ProArg: 2.955 ± 0.457
3.317ProSer: 3.317 ± 0.476
4.041ProThr: 4.041 ± 0.468
3.618ProVal: 3.618 ± 0.432
0.784ProTrp: 0.784 ± 0.233
1.628ProTyr: 1.628 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
3.558GlnAla: 3.558 ± 0.516
0.121GlnCys: 0.121 ± 0.085
1.387GlnAsp: 1.387 ± 0.401
1.869GlnGlu: 1.869 ± 0.309
0.965GlnPhe: 0.965 ± 0.21
2.774GlnGly: 2.774 ± 0.315
0.724GlnHis: 0.724 ± 0.184
2.231GlnIle: 2.231 ± 0.421
1.086GlnLys: 1.086 ± 0.238
3.498GlnLeu: 3.498 ± 0.44
0.784GlnMet: 0.784 ± 0.212
0.482GlnAsn: 0.482 ± 0.175
1.869GlnPro: 1.869 ± 0.41
1.447GlnGln: 1.447 ± 0.285
1.628GlnArg: 1.628 ± 0.312
1.689GlnSer: 1.689 ± 0.308
1.387GlnThr: 1.387 ± 0.304
2.171GlnVal: 2.171 ± 0.34
0.724GlnTrp: 0.724 ± 0.156
0.603GlnTyr: 0.603 ± 0.154
0.0GlnXaa: 0.0 ± 0.0
Arg
5.367ArgAla: 5.367 ± 0.657
0.724ArgCys: 0.724 ± 0.216
3.136ArgAsp: 3.136 ± 0.331
4.764ArgGlu: 4.764 ± 0.655
1.869ArgPhe: 1.869 ± 0.352
4.644ArgGly: 4.644 ± 0.521
0.844ArgHis: 0.844 ± 0.199
2.774ArgIle: 2.774 ± 0.418
3.739ArgLys: 3.739 ± 0.648
5.85ArgLeu: 5.85 ± 0.641
1.689ArgMet: 1.689 ± 0.29
2.352ArgAsn: 2.352 ± 0.425
2.593ArgPro: 2.593 ± 0.377
2.171ArgGln: 2.171 ± 0.352
4.885ArgArg: 4.885 ± 0.6
3.618ArgSer: 3.618 ± 0.436
3.015ArgThr: 3.015 ± 0.458
5.126ArgVal: 5.126 ± 0.53
1.387ArgTrp: 1.387 ± 0.3
1.568ArgTyr: 1.568 ± 0.25
0.0ArgXaa: 0.0 ± 0.0
Ser
5.91SerAla: 5.91 ± 0.765
0.422SerCys: 0.422 ± 0.171
3.076SerAsp: 3.076 ± 0.397
3.92SerGlu: 3.92 ± 0.546
2.292SerPhe: 2.292 ± 0.404
6.272SerGly: 6.272 ± 0.682
1.508SerHis: 1.508 ± 0.308
2.895SerIle: 2.895 ± 0.431
3.015SerLys: 3.015 ± 0.387
5.126SerLeu: 5.126 ± 0.501
1.447SerMet: 1.447 ± 0.278
2.352SerAsn: 2.352 ± 0.382
3.377SerPro: 3.377 ± 0.463
1.93SerGln: 1.93 ± 0.332
2.955SerArg: 2.955 ± 0.436
3.317SerSer: 3.317 ± 0.627
2.774SerThr: 2.774 ± 0.472
3.92SerVal: 3.92 ± 0.547
1.387SerTrp: 1.387 ± 0.27
1.508SerTyr: 1.508 ± 0.275
0.0SerXaa: 0.0 ± 0.0
Thr
6.392ThrAla: 6.392 ± 0.687
0.181ThrCys: 0.181 ± 0.102
4.221ThrAsp: 4.221 ± 0.567
4.342ThrGlu: 4.342 ± 0.511
1.869ThrPhe: 1.869 ± 0.35
6.091ThrGly: 6.091 ± 0.585
1.266ThrHis: 1.266 ± 0.304
2.774ThrIle: 2.774 ± 0.572
2.593ThrLys: 2.593 ± 0.372
5.669ThrLeu: 5.669 ± 0.609
1.086ThrMet: 1.086 ± 0.251
1.568ThrAsn: 1.568 ± 0.294
3.679ThrPro: 3.679 ± 0.544
1.869ThrGln: 1.869 ± 0.351
3.679ThrArg: 3.679 ± 0.469
3.437ThrSer: 3.437 ± 0.551
4.644ThrThr: 4.644 ± 0.582
5.488ThrVal: 5.488 ± 0.652
1.086ThrTrp: 1.086 ± 0.229
1.93ThrTyr: 1.93 ± 0.313
0.0ThrXaa: 0.0 ± 0.0
Val
6.634ValAla: 6.634 ± 0.698
0.362ValCys: 0.362 ± 0.151
5.066ValAsp: 5.066 ± 0.492
5.367ValGlu: 5.367 ± 0.56
2.352ValPhe: 2.352 ± 0.357
5.186ValGly: 5.186 ± 0.693
1.568ValHis: 1.568 ± 0.302
3.679ValIle: 3.679 ± 0.47
3.136ValLys: 3.136 ± 0.463
4.644ValLeu: 4.644 ± 0.472
1.206ValMet: 1.206 ± 0.371
2.352ValAsn: 2.352 ± 0.346
3.98ValPro: 3.98 ± 0.464
2.111ValGln: 2.111 ± 0.384
4.704ValArg: 4.704 ± 0.57
5.005ValSer: 5.005 ± 0.497
4.945ValThr: 4.945 ± 0.594
5.066ValVal: 5.066 ± 0.536
1.146ValTrp: 1.146 ± 0.307
2.171ValTyr: 2.171 ± 0.348
0.0ValXaa: 0.0 ± 0.0
Trp
1.869TrpAla: 1.869 ± 0.373
0.241TrpCys: 0.241 ± 0.102
1.447TrpAsp: 1.447 ± 0.278
1.025TrpGlu: 1.025 ± 0.244
0.844TrpPhe: 0.844 ± 0.196
1.628TrpGly: 1.628 ± 0.282
0.482TrpHis: 0.482 ± 0.172
1.266TrpIle: 1.266 ± 0.249
0.422TrpLys: 0.422 ± 0.147
1.689TrpLeu: 1.689 ± 0.278
0.422TrpMet: 0.422 ± 0.139
0.543TrpAsn: 0.543 ± 0.197
1.086TrpPro: 1.086 ± 0.237
0.965TrpGln: 0.965 ± 0.209
1.146TrpArg: 1.146 ± 0.269
0.965TrpSer: 0.965 ± 0.238
1.387TrpThr: 1.387 ± 0.342
1.93TrpVal: 1.93 ± 0.301
0.543TrpTrp: 0.543 ± 0.195
0.241TrpTyr: 0.241 ± 0.115
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.653TyrAla: 2.653 ± 0.419
0.181TyrCys: 0.181 ± 0.101
1.146TyrAsp: 1.146 ± 0.264
2.352TyrGlu: 2.352 ± 0.345
0.482TyrPhe: 0.482 ± 0.139
3.136TyrGly: 3.136 ± 0.416
0.724TyrHis: 0.724 ± 0.208
1.689TyrIle: 1.689 ± 0.311
1.447TyrLys: 1.447 ± 0.307
2.473TyrLeu: 2.473 ± 0.365
0.422TyrMet: 0.422 ± 0.122
1.025TyrAsn: 1.025 ± 0.284
1.508TyrPro: 1.508 ± 0.313
1.025TyrGln: 1.025 ± 0.211
2.774TyrArg: 2.774 ± 0.409
1.628TyrSer: 1.628 ± 0.282
2.05TyrThr: 2.05 ± 0.376
1.93TyrVal: 1.93 ± 0.274
0.603TyrTrp: 0.603 ± 0.188
0.422TyrTyr: 0.422 ± 0.13
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (16583 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski