Sewage-associated circular DNA virus-24

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UI46|A0A0B4UI46_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-24 OX=1592091 PE=4 SV=1
MM1 pKa = 7.37SRR3 pKa = 11.84SQHH6 pKa = 6.28LYY8 pKa = 8.63WIGTISIQSFPTWNPEE24 pKa = 3.85EE25 pKa = 4.73YY26 pKa = 10.37FSQTDD31 pKa = 3.3DD32 pKa = 3.43LQYY35 pKa = 10.81IRR37 pKa = 11.84GQKK40 pKa = 10.06EE41 pKa = 3.54EE42 pKa = 4.63GEE44 pKa = 4.63GGFQHH49 pKa = 6.14WQVLMVFSKK58 pKa = 10.57KK59 pKa = 9.9KK60 pKa = 7.89RR61 pKa = 11.84LSWITSRR68 pKa = 11.84TPGHH72 pKa = 6.59WEE74 pKa = 3.55PTRR77 pKa = 11.84SVAADD82 pKa = 3.12QYY84 pKa = 9.42VWKK87 pKa = 10.34EE88 pKa = 3.62EE89 pKa = 3.89TAIPNSRR96 pKa = 11.84FEE98 pKa = 4.01FGEE101 pKa = 3.54RR102 pKa = 11.84RR103 pKa = 11.84IRR105 pKa = 11.84RR106 pKa = 11.84NSKK109 pKa = 8.41TDD111 pKa = 3.1WAIVLQAARR120 pKa = 11.84EE121 pKa = 3.88RR122 pKa = 11.84RR123 pKa = 11.84LGDD126 pKa = 3.18IPEE129 pKa = 5.29DD130 pKa = 3.22IVVRR134 pKa = 11.84YY135 pKa = 8.61YY136 pKa = 11.7GNIIRR141 pKa = 11.84IGSDD145 pKa = 2.54NLRR148 pKa = 11.84PIEE151 pKa = 3.9QVRR154 pKa = 11.84TCKK157 pKa = 10.57VFWGLTGTGKK167 pKa = 9.99SRR169 pKa = 11.84RR170 pKa = 11.84AFEE173 pKa = 4.05EE174 pKa = 4.02AGMEE178 pKa = 4.33CYY180 pKa = 10.33VKK182 pKa = 10.73NPRR185 pKa = 11.84TKK187 pKa = 9.31WWDD190 pKa = 3.39GYY192 pKa = 11.16AGQKK196 pKa = 10.03NVVIDD201 pKa = 3.85EE202 pKa = 4.21FRR204 pKa = 11.84GGIDD208 pKa = 3.41VAYY211 pKa = 10.7LLLWLDD217 pKa = 3.75RR218 pKa = 11.84YY219 pKa = 10.53KK220 pKa = 11.24VIVEE224 pKa = 4.19KK225 pKa = 10.93KK226 pKa = 10.39GGALCLEE233 pKa = 4.85ALQFWITSNISPKK246 pKa = 10.36DD247 pKa = 3.47WYY249 pKa = 10.23PEE251 pKa = 4.25LDD253 pKa = 4.78DD254 pKa = 3.99EE255 pKa = 4.8TQGALRR261 pKa = 11.84RR262 pKa = 11.84RR263 pKa = 11.84LTEE266 pKa = 3.82VVHH269 pKa = 6.58FDD271 pKa = 3.46VAFEE275 pKa = 4.0

Molecular weight: 32.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UI46|A0A0B4UI46_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-24 OX=1592091 PE=4 SV=1
MM1 pKa = 7.97GYY3 pKa = 10.29RR4 pKa = 11.84SFVRR8 pKa = 11.84KK9 pKa = 9.1GARR12 pKa = 11.84GLKK15 pKa = 10.0RR16 pKa = 11.84GASRR20 pKa = 11.84YY21 pKa = 9.55RR22 pKa = 11.84SYY24 pKa = 11.45QSGARR29 pKa = 11.84THH31 pKa = 5.74KK32 pKa = 9.24TDD34 pKa = 4.04RR35 pKa = 11.84NSDD38 pKa = 3.38HH39 pKa = 7.02PAVKK43 pKa = 10.23GFRR46 pKa = 11.84TIAGMSRR53 pKa = 11.84KK54 pKa = 9.41ARR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84EE59 pKa = 3.68EE60 pKa = 3.7LGKK63 pKa = 10.66RR64 pKa = 11.84KK65 pKa = 9.49AHH67 pKa = 6.34LSKK70 pKa = 10.8AGQVKK75 pKa = 8.19NTTEE79 pKa = 4.06GVEE82 pKa = 4.38GAVHH86 pKa = 7.34NDD88 pKa = 3.59LQCLNVNFALGHH100 pKa = 5.97AKK102 pKa = 9.81PPKK105 pKa = 10.57GLGKK109 pKa = 9.49WNYY112 pKa = 7.3KK113 pKa = 9.94HH114 pKa = 6.81EE115 pKa = 4.49YY116 pKa = 8.98GVCLFADD123 pKa = 4.69LPMKK127 pKa = 10.48TGVQGQASLWTTCGVQNFIGAPTANNNSFEE157 pKa = 4.21WSGNPFDD164 pKa = 5.49LNPYY168 pKa = 9.67QYY170 pKa = 7.84TTGGVIQAASTDD182 pKa = 3.72KK183 pKa = 11.37NHH185 pKa = 6.44IQTDD189 pKa = 3.93VVRR192 pKa = 11.84MNSIKK197 pKa = 11.11GEE199 pKa = 4.18MNFTNLTVEE208 pKa = 4.25TNRR211 pKa = 11.84LDD213 pKa = 3.85VYY215 pKa = 10.85FMICSSNHH223 pKa = 5.65NLTVTQAWDD232 pKa = 3.78TILTSEE238 pKa = 5.32ALNQSAGSAATIVNGAVVAGYY259 pKa = 9.88VNPSSPFSGGSINSYY274 pKa = 10.41GEE276 pKa = 4.21EE277 pKa = 3.68FTKK280 pKa = 10.74YY281 pKa = 8.69YY282 pKa = 10.78RR283 pKa = 11.84SMRR286 pKa = 11.84KK287 pKa = 8.03FWKK290 pKa = 9.71IKK292 pKa = 10.42KK293 pKa = 9.99KK294 pKa = 9.62LTLCLSSGQTKK305 pKa = 9.91RR306 pKa = 11.84LRR308 pKa = 11.84YY309 pKa = 9.23NIPINKK315 pKa = 8.73VLHH318 pKa = 6.04KK319 pKa = 10.37EE320 pKa = 3.75VLIAMNSTGIQKK332 pKa = 10.12VKK334 pKa = 10.31GLTMEE339 pKa = 4.47CFVISRR345 pKa = 11.84GSPVKK350 pKa = 10.97VFDD353 pKa = 3.74TAGTTMSPALLEE365 pKa = 4.12YY366 pKa = 10.85GFTNLAQYY374 pKa = 9.88QFQACKK380 pKa = 10.11GAAEE384 pKa = 3.81RR385 pKa = 11.84LSFNRR390 pKa = 11.84QFEE393 pKa = 4.53GFVQEE398 pKa = 4.22ATAGASEE405 pKa = 4.34AFLNGADD412 pKa = 4.29IIDD415 pKa = 3.97SAVIATDD422 pKa = 3.16VLTTVAAAMVV432 pKa = 3.61

Molecular weight: 47.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

707

275

432

353.5

39.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.779 ± 1.732

1.414 ± 0.208

3.96 ± 0.962

5.799 ± 1.652

4.526 ± 0.105

8.487 ± 0.548

1.839 ± 0.247

5.233 ± 1.079

6.223 ± 0.495

6.648 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.98 ± 0.573

5.233 ± 1.497

3.112 ± 0.338

4.385 ± 0.221

6.789 ± 1.483

6.648 ± 0.769

6.931 ± 0.951

6.789 ± 0.157

2.546 ± 1.405

3.678 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski