Streptococcus phage 11865

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|E8ZDQ8|E8ZDQ8_9CAUD Phage protein OS=Streptococcus phage 11865 OX=870473 PE=4 SV=1
MM1 pKa = 7.92KK2 pKa = 10.19EE3 pKa = 3.58ILEE6 pKa = 4.43EE7 pKa = 3.94NLEE10 pKa = 4.11ASHH13 pKa = 7.68DD14 pKa = 4.04YY15 pKa = 10.94TSVLVVSLDD24 pKa = 3.24KK25 pKa = 11.36DD26 pKa = 3.74GEE28 pKa = 4.37INLGYY33 pKa = 10.32SWDD36 pKa = 3.9SSLQALGMLDD46 pKa = 3.33VAKK49 pKa = 10.77NYY51 pKa = 9.72ILNVINN57 pKa = 4.36

Molecular weight:
6.37 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|E8ZDQ1|E8ZDQ1_9CAUD Phage protein OS=Streptococcus phage 11865 OX=870473 PE=4 SV=1
MM1 pKa = 7.49QGILRR6 pKa = 11.84EE7 pKa = 4.05KK8 pKa = 9.96GQGIAEE14 pKa = 4.08RR15 pKa = 11.84AGEE18 pKa = 4.24GFEE21 pKa = 4.43LTVSPGQKK29 pKa = 9.27RR30 pKa = 11.84ANAKK34 pKa = 10.11ISTTDD39 pKa = 3.02IKK41 pKa = 11.66SMARR45 pKa = 11.84NKK47 pKa = 9.84KK48 pKa = 9.99HH49 pKa = 6.79NILLKK54 pKa = 10.84AMRR57 pKa = 4.66

Molecular weight:
6.28 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

9971

38

1421

207.7

23.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.73 ± 0.418

0.652 ± 0.111

5.897 ± 0.282

7.632 ± 0.509

4.313 ± 0.254

7.101 ± 0.474

1.524 ± 0.165

6.92 ± 0.382

8.093 ± 0.433

8.194 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.197

5.596 ± 0.331

2.708 ± 0.286

3.961 ± 0.18

4.684 ± 0.322

6.429 ± 0.373

5.626 ± 0.306

6.027 ± 0.283

1.494 ± 0.177

3.951 ± 0.245

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski