Microbotryum lychnidis-dioicae (strain p1A1 Lamole / MvSl-1064) (Anther smut fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Microbotryomycetes; Microbotryales; Microbotryaceae; Microbotryum; Microbotryum lychnidis-dioicae

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7656 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5GZG1|U5GZG1_USTV1 Uncharacterized protein OS=Microbotryum lychnidis-dioicae (strain p1A1 Lamole / MvSl-1064) OX=683840 GN=MVLG_00563 PE=3 SV=1
MM1 pKa = 7.55ILLALCLSVLYY12 pKa = 10.73GIDD15 pKa = 3.8DD16 pKa = 4.53PGVDD20 pKa = 3.47TVNKK24 pKa = 9.76LFEE27 pKa = 4.02EE28 pKa = 4.19ARR30 pKa = 11.84FAEE33 pKa = 4.43EE34 pKa = 4.01VVPPEE39 pKa = 3.97TTAEE43 pKa = 4.12SGSDD47 pKa = 3.32SSASYY52 pKa = 10.74YY53 pKa = 10.14PSSTNTNSSFKK64 pKa = 9.97TGSSQGDD71 pKa = 3.92TDD73 pKa = 5.21HH74 pKa = 7.05MDD76 pKa = 3.48LVV78 pKa = 4.02

Molecular weight: 8.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5HE39|U5HE39_USTV1 Uncharacterized protein OS=Microbotryum lychnidis-dioicae (strain p1A1 Lamole / MvSl-1064) OX=683840 GN=MVLG_05388 PE=4 SV=1
MM1 pKa = 7.8ASLLRR6 pKa = 11.84NVFSLTSRR14 pKa = 11.84TRR16 pKa = 11.84FTSARR21 pKa = 11.84SSPSSSTIPTVWRR34 pKa = 11.84SPSTSSISSSTPSAASSSLLSRR56 pKa = 11.84PFSSTPLSRR65 pKa = 11.84GLVRR69 pKa = 11.84KK70 pKa = 6.57PTKK73 pKa = 10.13IKK75 pKa = 10.95LKK77 pKa = 5.73THH79 pKa = 6.31KK80 pKa = 10.25GAAKK84 pKa = 9.84RR85 pKa = 11.84WFAIANGNFKK95 pKa = 10.46RR96 pKa = 11.84SQAGKK101 pKa = 8.26VHH103 pKa = 6.63LNGVLSPTRR112 pKa = 11.84LNRR115 pKa = 11.84LGKK118 pKa = 9.18PAFARR123 pKa = 11.84PIEE126 pKa = 4.29KK127 pKa = 10.26KK128 pKa = 9.95KK129 pKa = 10.6LRR131 pKa = 11.84RR132 pKa = 11.84LMPYY136 pKa = 10.27AA137 pKa = 4.46

Molecular weight: 14.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7354

302

7656

4103797

21

4834

536.0

58.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.523 ± 0.028

1.034 ± 0.011

5.449 ± 0.02

5.97 ± 0.028

3.37 ± 0.016

6.929 ± 0.023

2.404 ± 0.014

4.236 ± 0.016

4.503 ± 0.024

9.127 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.928 ± 0.008

3.111 ± 0.014

6.533 ± 0.03

3.756 ± 0.02

6.376 ± 0.021

9.708 ± 0.039

6.411 ± 0.02

6.12 ± 0.019

1.237 ± 0.009

2.276 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski