Pteropus vampyrus (Large flying fox)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Chiroptera; Megachiroptera;

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34802 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P3S107|A0A6P3S107_PTEVA bromodomain-containing protein 1-like OS=Pteropus vampyrus OX=132908 GN=LOC105311740 PE=4 SV=1
LL1 pKa = 6.28KK2 pKa = 9.62TLYY5 pKa = 10.01TCFTTVLLVSLPQIFQAHH23 pKa = 5.73EE24 pKa = 3.98KK25 pKa = 9.96AVSGLSVVLRR35 pKa = 11.84PPFVDD40 pKa = 5.25FLASGAQSIPNDD52 pKa = 3.48SVAGGEE58 pKa = 4.63GIHH61 pKa = 6.78SEE63 pKa = 4.27EE64 pKa = 3.95EE65 pKa = 3.84AFAVDD70 pKa = 5.89DD71 pKa = 4.32EE72 pKa = 5.77DD73 pKa = 6.26SDD75 pKa = 5.42GEE77 pKa = 4.49LNTWEE82 pKa = 4.22LSEE85 pKa = 4.89GVSSCPPKK93 pKa = 10.5EE94 pKa = 3.81QAADD98 pKa = 3.96LFNEE102 pKa = 4.26DD103 pKa = 3.11WDD105 pKa = 4.81LEE107 pKa = 4.38LKK109 pKa = 10.49ADD111 pKa = 3.94QGNPYY116 pKa = 10.37DD117 pKa = 4.73ADD119 pKa = 5.16DD120 pKa = 3.86IQGCISQEE128 pKa = 3.88VKK130 pKa = 10.33PWVCCAPQGDD140 pKa = 4.66MIYY143 pKa = 10.59DD144 pKa = 4.29PSWHH148 pKa = 6.9HH149 pKa = 6.75PPPLIPHH156 pKa = 5.6YY157 pKa = 10.76SKK159 pKa = 10.49MVFEE163 pKa = 5.05TGQFDD168 pKa = 4.21DD169 pKa = 6.01AEE171 pKa = 4.35DD172 pKa = 3.31

Molecular weight: 18.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P3RBJ1|A0A6P3RBJ1_PTEVA prolargin OS=Pteropus vampyrus OX=132908 GN=PRELP PE=4 SV=1
MM1 pKa = 7.57SSHH4 pKa = 5.15KK5 pKa = 8.91TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19489

15313

34802

21249595

31

34170

610.6

67.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.966 ± 0.014

2.16 ± 0.01

4.809 ± 0.008

7.231 ± 0.015

3.501 ± 0.009

6.426 ± 0.017

2.622 ± 0.007

4.247 ± 0.012

5.805 ± 0.016

9.843 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.006

3.603 ± 0.01

6.441 ± 0.019

4.942 ± 0.014

5.736 ± 0.013

8.635 ± 0.017

5.301 ± 0.011

5.905 ± 0.012

1.153 ± 0.005

2.536 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski