Bombyx mori (Silk moth)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura;

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14787 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9JEV5|H9JEV5_BOMMO Uncharacterized protein OS=Bombyx mori OX=7091 GN=101736352 PE=3 SV=1
MM1 pKa = 7.81AEE3 pKa = 3.71TMAYY7 pKa = 8.95EE8 pKa = 4.14PAMDD12 pKa = 4.84DD13 pKa = 3.29EE14 pKa = 5.28VYY16 pKa = 10.64DD17 pKa = 3.96YY18 pKa = 8.97DD19 pKa = 3.8TTKK22 pKa = 10.25IPEE25 pKa = 4.25IVTPDD30 pKa = 3.33DD31 pKa = 3.89MNEE34 pKa = 3.73SGYY37 pKa = 10.28QEE39 pKa = 4.77GSEE42 pKa = 3.91QSYY45 pKa = 11.04GDD47 pKa = 3.46RR48 pKa = 11.84RR49 pKa = 11.84DD50 pKa = 3.53SKK52 pKa = 11.15EE53 pKa = 3.92GFGGEE58 pKa = 4.25GDD60 pKa = 3.66SDD62 pKa = 4.04SGVDD66 pKa = 4.17GVSSGCSTADD76 pKa = 3.25DD77 pKa = 4.43CPVVSINDD85 pKa = 3.14VVAYY89 pKa = 9.58YY90 pKa = 10.61DD91 pKa = 3.43HH92 pKa = 6.65IRR94 pKa = 11.84PRR96 pKa = 11.84RR97 pKa = 11.84RR98 pKa = 11.84HH99 pKa = 4.78VPQLSFYY106 pKa = 9.38TVDD109 pKa = 3.28IEE111 pKa = 5.27RR112 pKa = 11.84MTLEE116 pKa = 5.64AIAQMYY122 pKa = 7.45EE123 pKa = 4.11CGVIVHH129 pKa = 6.33IQIQNTDD136 pKa = 3.35TEE138 pKa = 4.37VRR140 pKa = 11.84LFDD143 pKa = 3.5TT144 pKa = 5.01

Molecular weight: 16.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9J224|H9J224_BOMMO Protein kinase domain-containing protein OS=Bombyx mori OX=7091 PE=4 SV=1
MM1 pKa = 7.59EE2 pKa = 6.12DD3 pKa = 3.23EE4 pKa = 5.31AGLLLVLLRR13 pKa = 11.84LVLLRR18 pKa = 11.84LVVLRR23 pKa = 11.84LVLLRR28 pKa = 11.84LVVLRR33 pKa = 11.84LVLLRR38 pKa = 11.84LVVLRR43 pKa = 11.84LVLLRR48 pKa = 11.84LVVLRR53 pKa = 11.84LVLLRR58 pKa = 11.84LVLLRR63 pKa = 11.84LVLLRR68 pKa = 11.84LVLLRR73 pKa = 11.84LVLLRR78 pKa = 11.84LVLLRR83 pKa = 11.84LVLLRR88 pKa = 11.84LVLLRR93 pKa = 11.84LVLLRR98 pKa = 11.84LVLLRR103 pKa = 4.54

Molecular weight: 12.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14773

14

14787

5961797

9

18753

403.2

45.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.136 ± 0.041

1.991 ± 0.047

5.627 ± 0.017

6.72 ± 0.025

3.523 ± 0.018

5.712 ± 0.05

2.53 ± 0.013

5.328 ± 0.02

6.327 ± 0.032

9.051 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.21 ± 0.011

4.776 ± 0.024

5.397 ± 0.03

3.867 ± 0.02

5.731 ± 0.025

7.528 ± 0.025

5.766 ± 0.025

6.431 ± 0.017

1.115 ± 0.009

3.232 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski